المؤلفون: Phababpha, S, Kukongviriyapan, U, Pakdeechote, P, Senggunprai, L, Kukongviriyapan, V, Settasatian, C, Tatsanavivat, P, Intharaphet, P, Senthong, V, Komanasin, N, Settasatian, N, Greenwald, SE

مصطلحات موضوعية: Metabolic syndrome, Pulse wave velocity, Arterial stiffness, Chromosome 9p21.3, Single nucleotide polymorphism rs1333049

العلاقة: CARDIOVASCULAR DIABETOLOGY; http://qmro.qmul.ac.uk/jspui/handle/123456789/4720Test; 123456789/4720; ARTN 93; http://qmro.qmul.ac.uk/xmlui/handle/123456789/10751Test

الإتاحة: https://doi.org/10.1186/1475-2840-12-93Test
http://qmro.qmul.ac.uk/xmlui/handle/123456789/10751Test

المؤلفون: Prawan, A, Butsri, S, Kukongviriyapan, V, Senggunprai, L, Kongpetch, S

المصدر: 65th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA 2017) ; Planta Medica International Open ; ISSN 2509-6656

الإتاحة: https://doi.org/10.1055/s-0037-1608140Test

المؤلفون: Senggunprai, L, Klungsaeng, S, Kukongviriyapan, V, Prawan, A

المصدر: 65th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA 2017) ; Planta Medica International Open ; ISSN 2509-6656

الإتاحة: https://doi.org/10.1055/s-0037-1608139Test

المؤلفون: Kongpetch, S., Puapairoj, A., Ong, C. K., Senggunprai, L., Prawan, A., Kukongviriyapan, U., Chan‐On, W., Siew, E. Y., Khuntikeo, N., Teh, B. T., Kukongviriyapan, V.

المساهمون: National Medical Research Council, Thailand Research Fund, Khon Kaen University

المصدر: Cell Proliferation ; volume 49, issue 1, page 90-101 ; ISSN 0960-7722 1365-2184

الوصف: Objective Haem oxygenase‐1 ( HO ‐1) plays important roles in cytoprotection and tumour growth. Cholangiocarcinoma ( CCA ) is a deadly malignancy with very poor prognosis. The role of HO ‐1 in tumour progression in CCA up to now has been relatively unexplored, thus, its possible therapeutic implications in CCA have been investigated here. Materials and methods HO ‐1 expression in tumour tissues from 50 CCA patients was determined by immunohistochemical analysis and its association with survival time was evaluated using the Kaplan–Meier method. Its role in CCA cells in vitro was evaluated by transwell and wound healing assays and suppression of HO ‐1 expression by si RNA . Effects of HO ‐1 inhibition on gemicitabine ( GEM )‐mediated tumour suppression was evaluated in nude mice xenografted with CCA cells. Results HO ‐1 expression was inversely associated with median overall survival time. Hazard ratio of patients with high HO ‐1 expression was 2.42 (95% CI : 1.16–5.08) with reference to low expression and HO ‐1 knock‐down expression inhibited transwell cell migration. Suppression of HO ‐1 by Zn‐protoporphyrin (Zn PP ) enhanced cytotoxicity to GEM in CCA cells, validated in CCA xenografts. Treatment with GEM and Zn PP almost completely arrested tumour growth, whereas treatment with only a single reagent, retarded it. Tumour inhibition was associated with reduction in expression of Ki‐67 and microvascular density, and enhanced p53 and p21 immunohistochemical staining. Conclusion High HO ‐1 expression was associated with poor prognosis of CCA . Synergistic role of HO ‐1 inhibition in chemotherapy of CCA is a promising insight for treatment of this tumour and warrants further investigation.

الإتاحة: https://doi.org/10.1111/cpr.12228Test

المؤلفون: Sompar, N, Kukongviriyapan, V, Kukongviriyapan, U, Senggunprai, L, Prawan, A

المصدر: Tropical Journal of Pharmaceutical Research; Vol 14, No 5 (2015); 769-776 ; 1596-9827 ; 1596-5996

مصطلحات موضوعية: Chang hepatocyte, Curcumin, Tetrahydrocurcumin, Cytoprotection, Doxorubicin, Cadmium

الوصف: Purpose: To investigate the cytoprotective effect of tetrahydrocurcumin, (THC) and curcumin (CUR) on cytotoxicity induced by doxorubicin and cadmium in Chang liver cells.Methods: Cytotoxicity was determined by sulforhodamine B assay. The expression of nuclear factorerythroid- 2-related factor 2 (Nrf2) Nrf2 regulated cytoprotecetive enzymes, glutamylcysteine ligase catalytic subunit (GCLC) and NADP (H): quinone oxidoreductase1 (NQO1) was determined by Western blot analysis. Nuclear translocation of Nrf-2 was analyzed by immunofluorescence method. The level of superoxide formation was assayed by chemiluminescence method.Results: Treatment with THC or CUR significantly induced GCLC and NQO1 expression and the nuclear translocation of Nrf2. Exposure to doxorubicin (DOX) or Cd for 24 h induced cell death of about 50 %. However, pre-treatment with THC or CUR (1 or 6 μM) for 24 h significantly increased cell survival to 80 or 90 %, respectively (p < 0.05). Similar pre-treatment with THC or CUR significantly protected against Cd-induced cell death by a level of 80 and 85 %, respectively (p < 0.05). The cytoprotective effect of these compounds was associated with suppressed DOX- and Cd-induced superoxide formation and induction of GCLC and NQO1 expression.Conclusions: THC mediates its effects by activation of Nrf2 and its regulated enzymes, GCLC and NQO1. Induction of GCLC, NQO1 protein expression and suppression of superoxide are associated with the cytoprotective effect.Keywords: Chang hepatocyte, Curcumin, Tetrahydrocurcumin, Cytoprotection, Doxorubicin, Cadmium

وصف الملف: application/pdf

العلاقة: http://www.ajol.info/index.php/tjpr/article/view/122907/112449Test; http://www.ajol.info/index.php/tjpr/article/view/122907Test

الإتاحة: https://doi.org/10.4314/tjpr.v14i5.4Test
http://www.ajol.info/index.php/tjpr/article/view/122907Test