المؤلفون: Linz Fitzpatrick, Scott, Charlotte

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::e6e2b6e9e0e3edcbe91f9e81441ed52fTest

المؤلفون: Gómez-Gaviro, María Victoria, Scott, Charlotte E., Sesay, Abdul K., Matheu, Ander, Booth, Sarah, Galichet, Christophe, Lovell-Badge, Robin

المصدر: Proceedings of the National Academy of Sciences of the United States of America, 2012 Jan . 109(4), 1317-1322.

الوصول الحر: https://www.jstor.org/stable/41477244Test

المؤلفون: Scott, Charlotte, Zheng, Fang, De Baetselier, Patrick, Martens, Liesbet, Saeys, Yvan, De Prijck, Sofie, Lippens, Saskia, Abels, Chloé, Schoonooghe, Steve, Raes, Geert, Devoogdt, Nick, Lambrecht, Bart, Beschin, Alain, Guilliams, Martin

المصدر: NATURE COMMUNICATIONS ; ISSN: 2041-1723

مصطلحات موضوعية: Medicine and Health Sciences, TISSUE-RESIDENT MACROPHAGES, HEMATOPOIETIC STEM-CELLS, CARDIAC MACROPHAGES, FETAL MONOCYTES, LIVER, INFLAMMATION, INTESTINE, MICROGLIA, IDENTITY, RECEPTOR

الوصف: Self-renewing tissue-resident macrophages are thought to be exclusively derived from embryonic progenitors. However, whether circulating monocytes can also give rise to such macrophages has not been formally investigated. Here we use a new model of diphtheria toxin-mediated depletion of liver-resident Kupffer cells to generate niche availability and show that circulating monocytes engraft in the liver, gradually adopt the transcriptional profile of their depleted counterparts and become long-lived self-renewing cells. Underlining the physiological relevance of our findings, circulating monocytes also contribute to the expanding pool of macrophages in the liver shortly after birth, when macrophage niches become available during normal organ growth. Thus, like embryonic precursors, monocytes can and do give rise to self-renewing tissue-resident macrophages if the niche is available to them.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/7102628Test; http://hdl.handle.net/1854/LU-7102628Test; http://dx.doi.org/10.1038/ncomms10321Test; https://biblio.ugent.be/publication/7102628/file/7102644Test

الإتاحة: https://doi.org/10.1038/ncomms10321Test
https://biblio.ugent.be/publication/7102628Test
http://hdl.handle.net/1854/LU-7102628Test
https://biblio.ugent.be/publication/7102628/file/7102644Test

المؤلفون: Bain, Calum C, Hawley, Catherine A, Garner, Hannah, Scott, Charlotte, Schridde, Anika, Steers, Nicholas J, Mack, Matthias, Joshi, Anagha, Guilliams, Martin, Mowat, Allan Mc I, Geissmann, Frederic, Jenkins, Stephen J

المصدر: NATURE COMMUNICATIONS ; ISSN: 2041-1723

مصطلحات موضوعية: Medicine and Health Sciences, DENDRITIC CELLS, PERITONEAL-MACROPHAGES, HEMATOPOIETIC STEM-CELLS, TISSUE-RESIDENT MACROPHAGES, CIRCULATING MONOCYTES, ALVEOLAR MACROPHAGES, CARDIAC MACROPHAGES, ACUTE-INFLAMMATION, FETAL MONOCYTES, STEADY-STATE

الوصف: Peritoneal macrophages are one of the most studied macrophage populations in the body, yet the composition, developmental origin and mechanisms governing the maintenance of this compartment are controversial. Here we show resident F4/80(hi)GATA6(+) macrophages are long-lived, undergo non-stochastic self-renewal and retain cells of embryonic origin for at least 4 months in mice. However, Ly6C(+) monocytes constitutively enter the peritoneal cavity in a CCR2-dependent manner, where they mature into short-lived F4/80(lo)MHCII(+) cells that act, in part, as precursors of F4/80(hi)GATA6(+) macrophages. Notably, monocyte-derived F4/80(hi) macrophages eventually displace the embryonic population with age in a process that is highly gender dependent and not due to proliferative exhaustion of the incumbent embryonic population, despite the greater proliferative activity of newly recruited cells. Furthermore, although monocyte-derived cells acquire key characteristics of the embryonic population, expression of Tim4 was impaired, leading to cumulative changes in the population with age.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/8038274Test; http://hdl.handle.net/1854/LU-8038274Test; http://dx.doi.org/10.1038/ncomms11852Test; https://biblio.ugent.be/publication/8038274/file/8038288Test

الإتاحة: https://doi.org/10.1038/ncomms11852Test
https://biblio.ugent.be/publication/8038274Test
http://hdl.handle.net/1854/LU-8038274Test
https://biblio.ugent.be/publication/8038274/file/8038288Test

المؤلفون: Laoui, Damya, Keirsse, Jiri, Morias, Yannick, Van Overmeire, Eva, Geeraerts, Xenia, Elkrim, Yvon, Kiss, Mate, Bolli, Evangelia, Lahmar, Qods, Sichien, Dorine, Serneels, Jens, Scott, Charlotte, Boon, Louis, De Baetselier, Patrick, Mazzone, Massimiliano, Guilliams, Martin, Van Ginderachter, Jo A.

المصدر: NATURE COMMUNICATIONS ; ISSN: 2041-1723

مصطلحات موضوعية: Biology and Life Sciences, Medicine and Health Sciences, CYTOKINE GM-CSF, T-CELLS, INTRATUMORAL DELIVERY, CANCER-IMMUNOTHERAPY, STEADY-STATE, IN-VIVO, RESPONSES, SUBSETS, MACROPHAGES, DIFFERENTIATION

الوصف: Various steady state and inflamed tissues have been shown to contain a heterogeneous DC population consisting of developmentally distinct subsets, including cDC1s, cDC2s and monocyte-derived DCs, displaying differential functional specializations. The identification of functionally distinct tumour-associated DC (TADC) subpopulations could prove essential for the understanding of basic TADC biology and for envisaging targeted immunotherapies. We demonstrate that multiple mouse tumours as well as human tumours harbour ontogenically discrete TADC subsets. Monocyte-derived TADCs are prominent in tumour antigen uptake, but lack strong T-cell stimulatory capacity due to NO-mediated immunosuppression. Pre-cDC-derived TADCs have lymph node migratory potential, whereby cDC1s efficiently activate CD8(+) Tcells and cDC2s induce Th17 cells. Mice vaccinated with cDC2s displayed a reduced tumour growth accompanied by a reprogramming of pro-tumoural TAMs and a reduction of MDSCs, while cDC1 vaccination strongly induces anti-tumour CTLs. Our data might prove important for therapeutic interventions targeted at specific TADC subsets or their precursors.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/8507540Test; http://hdl.handle.net/1854/LU-8507540Test; http://dx.doi.org/10.1038/ncomms13720Test; https://biblio.ugent.be/publication/8507540/file/8507542Test

الإتاحة: https://doi.org/10.1038/ncomms13720Test
https://biblio.ugent.be/publication/8507540Test
http://hdl.handle.net/1854/LU-8507540Test
https://biblio.ugent.be/publication/8507540/file/8507542Test

المؤلفون: Guilliams, Martin, Dutertre, Charles-Antoine, Scott, Charlotte L, McGovern, Naomi, Sichien, Dorine, Chakarov, Svetoslav, Van Gassen, Sofie, Chen, Jinmiao, Poidinger, Michael, De Prijck, Sofie, Tavernier, Simon J, Low, Ivy, Irac, Sergio Erdal, Mattar, Citra Nurfarah, Sumatoh, Hermi Rizal, Low, Gillian Hui Ling, Chung, Tam John Kit, Chan, Dedrick Kok Hong, Tan, Ker Kan, Hon, Tony Lim Kiat, Fossum, Even, Bogen, Bjarne, Choolani, Mahesh, Chan, Jerry Kok Yen, Larbi, Anis, Luche, Hervé, Henri, Sandrine, Saeys, Yvan, Newell, Evan William, Lambrecht, Bart N, Malissen, Bernard, Ginhoux, Florent

مصطلحات موضوعية: Animals, Cell Differentiation, Dendritic Cells, Flow Cytometry, Humans, Inflammation, Macaca, Mice, Inbred C57BL

الوصف: Dendritic cells (DCs) are professional antigen-presenting cells that hold great therapeutic potential. Multiple DC subsets have been described, and it remains challenging to align them across tissues and species to analyze their function in the absence of macrophage contamination. Here, we provide and validate a universal toolbox for the automated identification of DCs through unsupervised analysis of conventional flow cytometry and mass cytometry data obtained from multiple mouse, macaque, and human tissues. The use of a minimal set of lineage-imprinted markers was sufficient to subdivide DCs into conventional type 1 (cDC1s), conventional type 2 (cDC2s), and plasmacytoid DCs (pDCs) across tissues and species. This way, a large number of additional markers can still be used to further characterize the heterogeneity of DCs across tissues and during inflammation. This framework represents the way forward to a universal, high-throughput, and standardized analysis of DC populations from mutant mice and human patients.

وصف الملف: Print-Electronic; application/pdf

العلاقة: https://www.repository.cam.ac.uk/handle/1810/277631Test

الإتاحة: https://doi.org/10.17863/CAM.24960Test
https://www.repository.cam.ac.uk/handle/1810/277631Test

المؤلفون: Bain, Calum C., Hawley, Catherine A., Garner, Hannah, Scott, Charlotte L., Schridde, Anika, Steers, Nicholas J., Mack, Matthias, Joshi, Anagha, Guilliams, Martin, Mowat, Allan, Geissmann, Frederic, Jenkins, Stephen J.

الوصف: Peritoneal macrophages are one of the most studied macrophage populations in the body, yet the composition, developmental origin and mechanisms governing the maintenance of this compartment are controversial. Here we show resident F4/80hiGATA6+ macrophages are long-lived, undergo non-stochastic self-renewal and retain cells of embryonic origin for at least 4 months in mice. However, Ly6C+ monocytes constitutively enter the peritoneal cavity in a CCR2-dependent manner, where they mature into short-lived F4/80loMHCII+ cells that act, in part, as precursors of F4/80hiGATA6+ macrophages. Notably, monocyte-derived F4/80hi macrophages eventually displace the embryonic population with age in a process that is highly gender dependent and not due to proliferative exhaustion of the incumbent embryonic population, despite the greater proliferative activity of newly recruited cells. Furthermore, although monocyte-derived cells acquire key characteristics of the embryonic population, expression of Tim4 was impaired, leading to cumulative changes in the population with age.

وصف الملف: text

العلاقة: https://eprints.gla.ac.uk/121159/1/121159.pdfTest; Bain, C. C. et al. (2016) Long-lived self-renewing bone marrow-derived macrophages displace embryo-derived cells to inhabit adult serous cavities. Nature Communications , 7, ncomms1185. (doi:10.1038/ncomms11852 ) (PMID:27292029) (PMCID:PMC4910019)

الإتاحة: https://doi.org/10.1038/ncomms11852Test
https://eprints.gla.ac.uk/121159Test/
https://eprints.gla.ac.uk/121159/1/121159.pdfTest

المؤلفون: Scott, Charlotte, Soen, Bieke, Martens, Liesbet, Skrypek, Nicolas, Saelens, Wouter, Taminau, Joachim, Blancke, Gillian, Van Isterdael, Gert, Huylebroeck, Danny, Haigh, Jody, Saeys, Yvan, Guilliams, Martin, Lambrecht, Bart, Berx, Geert

المصدر: JOURNAL OF EXPERIMENTAL MEDICINE ; ISSN: 0022-1007

مصطلحات موضوعية: Medicine and Health Sciences, FACTOR-BINDING SITES, TERMINAL DIFFERENTIATION, INTESTINAL LAMINA PROPRIA, DENDRITIC CELL-DEVELOPMENT, CLONOGENIC PROGENITOR, SUBSET DEVELOPMENT, SIGNALING CONTROLS, BONE-MARROW, FACTOR E2-2, IN-VIVO

الوصف: Plasmacytoid dendritic cells (DCs [pDCs]) develop from pre-pDCs, whereas two lineages of conventional DCs (cDCs; cDC1s and cDC2s) develop from lineage-committed pre-cDCs. Several transcription factors (TFs) have been implicated in regulating the development of pDCs (E2-2 and Id2) and cDC1s (Irf8, Id2, and Batf3); however, those required for the early commitment of pre-cDCs toward the cDC2 lineage are unknown. Here, we identify the TF zinc finger E box-binding homeobox 2 (Zeb2) to play a crucial role in regulating DC development. Zeb2 was expressed from the pre-pDC and pre-cDC stage onward and highly expressed in mature pDCs and cDC2s. Mice conditionally lacking Zeb2 in CD11c(+) cells had a cell-intrinsic reduction in pDCs and cDC2s, coupled with an increase in cDC1s. Conversely, mice in which CD11c(+) cells overexpressed Zeb2 displayed a reduction in cDC1s. This was accompanied by altered expression of Id2, which was up-regulated in cDC2s and pDCs from conditional knock-out mice. Zeb2 chromatin immunoprecipitation analysis revealed Id2 to be a direct target of Zeb2. Thus, we conclude that Zeb2 regulates commitment to both the cDC2 and pDC lineages through repression of Id2.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/8055512Test; http://hdl.handle.net/1854/LU-8055512Test; http://dx.doi.org/10.1084/jem.20151715Test; https://biblio.ugent.be/publication/8055512/file/8055531Test

الإتاحة: https://doi.org/10.1084/jem.20151715Test
https://biblio.ugent.be/publication/8055512Test
http://hdl.handle.net/1854/LU-8055512Test
https://biblio.ugent.be/publication/8055512/file/8055531Test

المؤلفون: van de Laar, Lianne, Saelens, Wouter, De Prijck, Sofie, Martens, Liesbet, Scott, Charlotte L., Van Isterdael, Gert, Hoffmann, Eik, Beyaert, Rudi, Saeys, Yvan, Lambrecht, Bart N., Guilliams, Martin

المصدر: Immunity ; volume 44, issue 4, page 755-768 ; ISSN 1074-7613

مصطلحات موضوعية: Infectious Diseases, Immunology, Immunology and Allergy

الإتاحة: https://doi.org/10.1016/j.immuni.2016.02.017Test
https://api.elsevier.com/content/article/PII:S1074761316300553?httpAccept=text/plainTest
https://api.elsevier.com/content/article/PII:S1074761316300553?httpAccept=text/xmlTest

المؤلفون: Sichien, Dorine, Scott, Charlotte L., Martens, Liesbet, Vanderkerken, Matthias, Van Gassen, Sofie, Plantinga, Maud, Joeris, Thorsten, De Prijck, Sofie, Vanhoutte, Leen, Vanheerswynghels, Manon, Van Isterdael, Gert, Toussaint, Wendy, Madeira, Filipe Branco, Vergote, Karl, Agace, William W., Clausen, Björn E., Hammad, Hamida, Dalod, Marc, Saeys, Yvan, Lambrecht, Bart N., Guilliams, Martin

المصدر: Immunity ; volume 45, issue 3, page 626-640 ; ISSN 1074-7613

الإتاحة: https://doi.org/10.1016/j.immuni.2016.08.013Test
https://api.elsevier.com/content/article/PII:S1074761316303375?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S1074761316303375?httpAccept=text/plainTest