المؤلفون: Louwe, Pieter A., Scott, Charlotte L.

المصدر: Science Immunology ; volume 6, issue 64 ; ISSN 2470-9468

مصطلحات موضوعية: General Medicine, Immunology

الوصف: The transcription factor c-MAF regulates perivascular macrophage phenotypes across tissues, including white adipose tissue, where its loss from murine vasculature–associated macrophages leads to increased vascularization and protection from metabolic syndrome (see the related Research Article by Moura Silva et al . ).

الإتاحة: https://doi.org/10.1126/sciimmunol.abl5793Test

المؤلفون: Scott, Charlotte, Murray, Zangerle T. F. P., Beckham, Katherine S. H., Douce, Gillian, Mowat, Allan McI

المصدر: EUROPEAN JOURNAL OF IMMUNOLOGY ; ISSN: 0014-2980 ; ISSN: 1521-4141

مصطلحات موضوعية: Biology and Life Sciences, Medicine and Health Sciences, LYMPHOID-TISSUES, T-CELL, DENDRITIC CELLS, SIRP alpha, Intestine, Homeostasis, Development, GUT, MUTANT MICE, IN-VIVO, ESSENTIAL ROLES, ORAL TOLERANCE, DIFFERENTIATION, RETINOIC-ACID

الوصف: Signal regulatory protein alpha (SIRP alpha/CD172a) is a conserved transmembrane protein thought to play an inhibitory role in immune function by binding the ubiquitous ligand CD47. SIRP alpha expression has been used to identify dendritic cell subsets across species and here we examined its expression and function on intestinal DCs in mice. Normal mucosa contains four subsets of DCs based on their expression of CD103 and CD11b and three of these express SIRP alpha. However, loss of SIRP alpha signaling in mice leads to a selective reduction in the CD103(+)CD11b(+) subset of DCs in the small intestine, colon, and amongmigratory DCs in the mesenteric lymph node. In parallel, these mice have reduced numbers of T(H)17 cells in steady-state intestinal mucosa, and a defective T(H)17 response to Citrobacter infection. Identical results were obtained in CD47KO mice. DC precursors from SIRP alpha mutant mice had an enhanced ability to generate CD103(+)CD11b(+) DCs in vivo, but CD103(+)CD11b(+) DCs from mutant mice were more prone to die by apoptosis. These data show a previously unappreciated and crucial role for SIRP alpha in the homeostasis of CD103(+)CD11b(+) DCs in the intestine, as well as providing further evidence that this subset of DCs is critical for the development of mucosal T(H)17 responses.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/6848919Test; http://hdl.handle.net/1854/LU-6848919Test; http://dx.doi.org/10.1002/eji.201444859Test; https://biblio.ugent.be/publication/6848919/file/8716194Test

الإتاحة: https://doi.org/10.1002/eji.201444859Test
https://biblio.ugent.be/publication/6848919Test
http://hdl.handle.net/1854/LU-6848919Test
https://biblio.ugent.be/publication/6848919/file/8716194Test

المؤلفون: Sauter, Kristin A., Pridans, Clare, Sehgal, Anuj, Bain, Calum C., Scott, Charlotte, Moffat, Lindsey, Rojo, Rocio, Stutchfield, Ben M., Davies, Claire L., Donaldson, David S., Renault, Kathleen, McColl, Barry W., Mowat, Alan M., Serrels, Alan, Frame, Margaret C., Mabbott, Neil A., Hume, David A.

المصدر: PLOS ONE ; ISSN: 1932-6203

مصطلحات موضوعية: Biology and Life Sciences, Medicine and Health Sciences, COLONY-STIMULATING FACTOR, MONONUCLEAR PHAGOCYTE SYSTEM, ISOLATED, LYMPHOID FOLLICLES, INTESTINAL LAMINA PROPRIA, HEMATOPOIETIC STEM-CELLS, FACTOR-I CSF-1, FACTOR-1 RECEPTOR, T-CELLS, IMMUNOHISTOCHEMICAL, LOCALIZATION, DIFFERENTIAL TRANSCRIPTION

الوصف: The MacBlue transgenic mouse uses the Csf1r promoter and first intron to drive expression of gal4-VP16, which in turn drives a cointegrated gal4-responsive UAS-ECFP cassette. The Csf1r promoter region used contains a deletion of a 150 bp conserved region covering trophoblast and osteoclast-specific transcription start sites. In this study, we examined expression of the transgene in embryos and adult mice. In embryos, ECFP was expressed in the large majority of macrophages derived from the yolk sac, and as the liver became a major site of monocytopoiesis. In adults, ECFP was detected at high levels in both Ly6C(+) and Ly6C(-) monocytes and distinguished them from Ly6C(+), F4/80(+), CSF1R(+) immature myeloid cells in peripheral blood. ECFP was also detected in the large majority of microglia and Langerhans cells. However, expression was lost from the majority of tissue macrophages, including Kupffer cells in the liver and F4/80(+) macrophages of the lung, kidney, spleen and intestine. The small numbers of positive cells isolated from the liver resembled blood monocytes. In the gut, ECFP+ cells were identified primarily as classical dendritic cells or blood monocytes in disaggregated cell preparations. Immunohistochemistry showed large numbers of ECFP+ cells in the Peyer's patch and isolated lymphoid follicles. The MacBlue transgene was used to investigate the effect of treatment with CSF1-Fc, a form of the growth factor with longer half-life and efficacy. CSF1-Fc massively expanded both the immature myeloid cell (ECFP-) and Ly6C(+) monocyte populations, but had a smaller effect on Ly6C(-) monocytes. There were proportional increases in ECFP+ cells detected in lung and liver, consistent with monocyte infiltration, but no generation of ECFP+ Kupffer cells. In the gut, there was selective infiltration of large numbers of cells into the lamina propria and Peyer's patches. We discuss the use of the MacBlue transgene as a marker of monocyte/macrophage/dendritic cell differentiation.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/8716082Test; http://hdl.handle.net/1854/LU-8716082Test; http://dx.doi.org/10.1371/journal.pone.0105429Test; https://biblio.ugent.be/publication/8716082/file/8716083Test

الإتاحة: https://doi.org/10.1371/journal.pone.0105429Test
https://biblio.ugent.be/publication/8716082Test
http://hdl.handle.net/1854/LU-8716082Test
https://biblio.ugent.be/publication/8716082/file/8716083Test

المؤلفون: Mattei, Fabrizio, Sauter, Kristin A., Pridans, Clare, Sehgal, Anuj, Bain, Calum C., Scott, Charlotte, Moffat, Lindsey, Rojo, Rocío, Stutchfield, Ben M., Davies, Claire L., Donaldson, David S., Renault, Kathleen, McColl, Barry W., Mowat, Allan M., Serrels, Alan, Frame, Margaret C., Mabbott, Neil A., Hume, David A.

الوصف: The MacBlue transgenic mouse uses the Csf1r promoter and first intron to drive expression of gal4-VP16, which in turn drives a cointegrated gal4-responsive UAS-ECFP cassette. The Csf1r promoter region used contains a deletion of a 150 bp conserved region covering trophoblast and osteoclast-specific transcription start sites. In this study, we examined expression of the transgene in embryos and adult mice. In embryos, ECFP was expressed in the large majority of macrophages derived from the yolk sac, and as the liver became a major site of monocytopoiesis. In adults, ECFP was detected at high levels in both Ly6C+ and Ly6C- monocytes and distinguished them from Ly6C+, F4/80+, CSF1R+ immature myeloid cells in peripheral blood. ECFP was also detected in the large majority of microglia and Langerhans cells. However, expression was lost from the majority of tissue macrophages, including Kupffer cells in the liver and F4/80+ macrophages of the lung, kidney, spleen and intestine. The small numbers of positive cells isolated from the liver resembled blood monocytes. In the gut, ECFP+ cells were identified primarily as classical dendritic cells or blood monocytes in disaggregated cell preparations. Immunohistochemistry showed large numbers of ECFP+ cells in the Peyer's patch and isolated lymphoid follicles. The MacBlue transgene was used to investigate the effect of treatment with CSF1-Fc, a form of the growth factor with longer half-life and efficacy. CSF1-Fc massively expanded both the immature myeloid cell (ECFP−) and Ly6C+ monocyte populations, but had a smaller effect on Ly6C− monocytes. There were proportional increases in ECFP+ cells detected in lung and liver, consistent with monocyte infiltration, but no generation of ECFP+ Kupffer cells. In the gut, there was selective infiltration of large numbers of cells into the lamina propria and Peyer's patches. We discuss the use of the MacBlue transgene as a marker of monocyte/macrophage/dendritic cell differentiation.

وصف الملف: text

العلاقة: https://eprints.gla.ac.uk/97258/1/97258.pdfTest; Mattei, F. et al. (2014) The MacBlue binary transgene (csf1r-gal4VP16/UAS-ECFP) provides a novel marker for visualisation of subsets of monocytes, macrophages and dendritic cells and responsiveness to CSF1 administration. PLoS ONE , 9(8), e105429. (doi:10.1371/journal.pone.0105429 ) (PMID:25137049) (PMCID:PMC4138162)

الإتاحة: https://doi.org/10.1371/journal.pone.0105429Test
https://eprints.gla.ac.uk/97258Test/
https://eprints.gla.ac.uk/97258/1/97258.pdfTest

المؤلفون: Bain, Calum C, Bravo-Blas, Alberto, Scott, Charlotte L, Gomez Perdiguero, Elisa, Geissmann, Frederic, Henri, Sandrine, Malissen, Bernard, Osborne, Lisa C, Artis, David, Mowat, Allan McI

المصدر: Bain , C C , Bravo-Blas , A , Scott , C L , Gomez Perdiguero , E , Geissmann , F , Henri , S , Malissen , B , Osborne , L C , Artis , D & Mowat , A M 2014 , ' Constant replenishment from circulating monocytes maintains the macrophage pool in the intestine of adult mice ' , Nature Immunology , vol. 15 , no. 10 , pp. 929-937 . https://doi.org/10.1038/ni.2967Test

مصطلحات موضوعية: Animals, Newborn, Antigens, CD11b, Differentiation, Ly, Bone Marrow Transplantation, Cell Differentiation, Cell Proliferation, Flow Cytometry, Gene Expression, Intestinal Mucosa, Intestines, Macrophages, Mice, Inbred C57BL, Knockout, Transgenic, Models, Immunological, Monocytes, Parabiosis, Receptors, CCR2, Chemokine, Reverse Transcriptase Polymerase Chain Reaction, Time Factors

الوصف: The paradigm that macrophages that reside in steady-state tissues are derived from embryonic precursors has never been investigated in the intestine, which contains the largest pool of macrophages. Using fate-mapping models and monocytopenic mice, together with bone marrow chimera and parabiotic models, we found that embryonic precursor cells seeded the intestinal mucosa and demonstrated extensive in situ proliferation during the neonatal period. However, these cells did not persist in the intestine of adult mice. Instead, they were replaced around the time of weaning by the chemokine receptor CCR2-dependent influx of Ly6C(hi) monocytes that differentiated locally into mature, anti-inflammatory macrophages. This process was driven largely by the microbiota and had to be continued throughout adult life to maintain a normal intestinal macrophage pool.

وصف الملف: application/pdf

الإتاحة: https://doi.org/10.1038/ni.2967Test
https://kclpure.kcl.ac.uk/portal/en/publications/fddb010b-844e-4a9c-bdb1-2df52dc1c4c3Test
https://kclpure.kcl.ac.uk/ws/files/56792829/Constant_replenishment_from_circulating_BAIN_Oct_2014_GREEN_AAM.pdfTest

المؤلفون: Bain, Calum C, Bravo-Blas, Alberto, Scott, Charlotte L, Perdiguero, Elisa Gomez, Geissmann, Frederic, Henri, Sandrine, Malissen, Bernard, Osborne, Lisa C, Artis, David, Mowat, Allan McI

المصدر: Nature Immunology ; volume 15, issue 11, page 1090-1090 ; ISSN 1529-2908 1529-2916

مصطلحات موضوعية: Immunology, Immunology and Allergy

الإتاحة: https://doi.org/10.1038/ni1114-1090cTest
http://www.nature.com/articles/ni1114-1090c.pdfTest
http://www.nature.com/articles/ni1114-1090cTest

المؤلفون: Coventry, Peter, Panagioti, Maria, Scott, Charlotte, Blakemore, Amy

المصدر: International Journal of Chronic Obstructive Pulmonary Disease ; page 1289 ; ISSN 1178-2005

الإتاحة: https://doi.org/10.2147/copd.s72073Test
https://www.dovepress.com/getfile.php?fileID=22398Test

المؤلفون: Scott, Charlotte Angas

المصدر: Philosophical Transactions of the Royal Society of London. A, 1894 Jan 01. 185, 247-277.

الوصول الحر: https://www.jstor.org/stable/90671Test

المؤلفون: Scott, Charlotte Angas

المصدر: Annals of Mathematics, 1907 Apr 01. 8(3), 127-134.

الوصول الحر: https://www.jstor.org/stable/1967308Test

المؤلفون: Scott, Charlotte Angas

المصدر: Annals of Mathematics, 1900 Jan 01. 2(1/4), 64-72.

الوصول الحر: https://www.jstor.org/stable/2007183Test