المؤلفون: Heiser Arteaga-Pautt, O. Elias Bru-Cordero, Dina Ricardo-Caldera, Lyda Espitia-Pérez, Paula Avilés-Vergara, Catalina Tovar-Acero, Lorena Castaño-Caraballo, Sandra Janeth Perdomo-Lara, Helvey Ramón Zetién-Arteaga, Valentina Behaine-Bravo, Sara Cecilia Soto-De León

المصدر: BMC Infectious Diseases, Vol 24, Iss 1, Pp 1-10 (2024)

مصطلحات موضوعية: High-risk human papillomavirus (HR-HPV), Cervical cancer (CC), Pap smear test, Population, Colombia, Infectious and parasitic diseases, RC109-216

الوصف: SUMMARY Background Cervical cancer (CC) is a significant global public health concern, particularly in developing countries such as Colombia. The main risk factor involves high-risk HPV types (HR-HPV) infection, coupled with population-specific variables. The Caribbean region in Colombia lacks research on HR-HPV-type frequencies. Therefore, this study aims to establish the prevalence of type-specific HR-HPV and its association with sociodemographic factors among women undergoing cervical cytology screening. Methods A cross-sectional study involving voluntary women who provided informed consent and completed a questionnaire capturing sociodemographic, clinical, and sexual behavior information was conducted. All participants underwent cervical cytology and molecular analysis. Generic HPV detection employed three simultaneous PCRs (GP5+/6+, MY09/11, and PU1R/2 M), and positive samples were genotyped using the Optiplex HPV Genotyping kit. The analysis encompassed the 12 types of high-risk HPV (HR-HPV-16,-18,-31,-33,-35,-39,-45,-51,-52,-56,-58, and − 59). Frequencies were reported based on geographic subregions within the Córdoba department, and disparities were made between single and multiple infections. Sociodemographic and clinical variables were subjected to ordinal logistic regression, with statistical significance at a p-value

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2334Test

الوصول الحر: https://doaj.org/article/2c88aa58a6794bc4baeb0b10f2e946c3Test

المؤلفون: Martha Rebolledo, María Rosa Buenahora, Fabio Ancisar Aristizabal, David Díaz-Báez, Carlos M Ardila, Farith González-Martínez, Carlos Humberto Colegial, Efrain Alvarez, Sandra Janeth Perdomo-Lara, Jairo Bustillo, Alvaro Horta, Gloria Inés Lafaurie

المساهمون: Lafaurie, Gloria Ines [0000-0003-3986-0625], Perdomo Lara, Sandra Janneth [0000-0002-4429-3760], Díaz-Báez, David [0000-0001-8890-6250]

المصدر: Perdomo-Lara, Sandra Janeth ; Buenahora, María Rosa ; Alvarez Martínez, Efraín ; González Martínez, Farith ; Rebolledo Cobos, Martha ; Aristizábal Gutiérrez, Fabio Ancízar ; Colegial, Carlos Humberto ; Horta, Alvaro ; Bustillo Rojas, Jairo Alberto ; Díaz Báez, David ; Ardila Medina, Carlos Martín ; Lafaurie Villamil, Gloria Inés. Human papilloma virus genotypes in dysplasia and epithelial hyperplasia of oral cavity using the luminex xmap technology. A multicenter study. En: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, 25 1 2020: 1
RODERIC. Repositorio Institucional de la Universitat de Valéncia
instname
Repositorio U. El Bosque
Universidad El Bosque
instacron:Universidad El Bosque
Medicina Oral, Patología Oral y Cirugía Bucal
RODERIC: Repositorio Institucional de la Universitat de Valéncia

مصطلحات موضوعية: medicine.medical_specialty, Epithelial dysplasia, Genotype, Population, Gastroenterology, 03 medical and health sciences, 0302 clinical medicine, Epithelial hyperplasia, Internal medicine, Ensayo clínico, medicine, Humans, Papillomaviridae, education, General Dentistry, Oral Dysplasia, education.field_of_study, Oral Medicine and Pathology, Hyperplasia, biology, business.industry, Research, Papillomavirus Infections, Cancer, virus diseases, Electroforesis en gel bidimensional, 030206 dentistry, medicine.disease, biology.organism_classification, CIENCIAS MÉDICAS [UNESCO], female genital diseases and pregnancy complications, Enfermedades de la boca, Otorhinolaryngology, Dysplasia, UNESCO::CIENCIAS MÉDICAS, Surgery, HPV-genotypes, business

الوصف: Background Oral cancer associated with high risk (HPV-HR) human papilloma virus (HPV) has been increasing. HPV-HR has been associated with epithelial dysplasia, however, little information exists on its frequency in epithelial hyperplasia lesions. The aim of this study is to compare HPV genotypes in dysplastic and hyperplastic lesions of oral cavity. Material and Methods Two hundred and fifty oral lesions: 131 dysplasia and 119 hyperplasia from two regions of Colombia were evaluated. One hundred seventy-four coming from urban area and 104 from a high risk population to oral cancer from a rural area. HPV was identified by qPCR and Twenty-four HPVs genotypes were evaluated by Luminex® technology. Logistic regressions were performed to establish the associations between HPV infections with oral dysplasia. Results Twenty-eight percent (70/250) of the samples were positives for any HPV and HPV-HRs were more frequently than low risk HPVs. HPV-16 was the most detected genotype (16%) followed by HPV-31, 53, 18 and 45. HPV, HPV-HRs and HPV-16 were only associated with dysplasia in urban area; OR 3.28 (CI 95% 1.49-7.17), OR 7.94 (CI 95% 2.97-21.2) and OR 5.90 (CI 95% 2.05-17). Individuals in rural area showed more HPV and HPV-HRs infection in hyperplasic lesions than urban population. The majority of HPV+ lesions had multi-type of HPV (52/70) and the urban individuals showed more genotypes than rural population. Conclusions HPV-.HRs are frequently found in hyperplastic and dysplastic epithelial lesions. HPV-HRs and HPV-16 were associated with dysplasia in urban population. Rural high risk population and urban population differ in the frequency and variety of HPV genotypes. Key words:Human papilloma virus, epithelial dysplasia, epithelial hyperplasia, HPV-genotypes.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::faa075e5fbf2b82453c9d0bb23f20757Test
https://hdl.handle.net/10550/77072Test

المؤلفون: Carlos Corredor Pereira, Sandra Janeth Perdomo Lara, Jorge Arturo Rey Cubillos, Marcela Godoy Corredor, Ospino Benjamín

المصدر: Revista Med; Vol. 20 No. 1 (2012): january-june; 27-34
Revista Med; Vol. 20 Núm. 1 (2012): enero-junio; 27-34

مصطلحات موضوعية: células madre mesenquimales, colágeno tipo 1, osteocalcina, diferenciación osteogénica, Ocean Engineering, Safety, Risk, Reliability and Quality, mecanotransducción

الوصف: El papel de la estimulación mecánica en la diferenciación de las células madre mesenquimales humanas (CMMHs) es una alternativa terapéutica para aplicaciones en ingeniería tisular. Este estudio evaluó el efecto de cargas mecánicas sobre la diferenciación de las CMMHs, y los mecanismos celulares que intervienen en el proceso de mecanotransducción. Las CMMHs se sembraron en frascos de cultivo de 75cm2 y fueron expuestas a tensión uniaxial de deformación de 500, 1000, 1500 y 2000 micro strains (μH), con una intensidad de 9 ciclos/minuto por 3 horas durante 4 días consecutivos. Se evaluó la actividad transcripcional de los factores de transcripción Runx2 y Sox9 y de los genes de Osteocalcina (OC), Colágeno tipo 1 (Col-1) y Fosfatasa Alcalina (ALP). Después de la exposición al estímulo, los marcadores osteogénicos Col-1, OC, y ALP se expresaron temporalmente; y los factores de transcripción Runx2 y Sox9 disminuyeron la expresión con respecto a las células de grupo control (sin estímulo), sugiriendo que el estímulo mecánico indujo la diferenciación de las células CMMHs a linaje osteoblástico. La identificación de los genes que traducen los estímulos mecánicos en las CMMHs y modulan la diferenciación osteogénica, tienen proyección directa en medicina regenerativa a través del desarrollo y perfeccionamiento del enfoque de ingeniería de tejidos funcionales.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0f909c44b4224a876b228c74a9885aeTest
https://doi.org/10.18359/rmed.1208Test