المؤلفون: Hao, M., Franqui-Machin, R., Xu, H., Shaughnessy, J., Jr., Barlogie, B., Roodman, D., Quelle, D. E., Janz, S., Tomasson, M. H., Sanderson, R. D., Qiu, L., Frech, I., Tricot, G., Zhan, F.

المساهمون: Medicine, School of Medicine

المصدر: PMC

مصطلحات موضوعية: Osteoclasts, Multiple myeloma, Bone marrow -- Diseases

وصف الملف: application/pdf

العلاقة: Leukemia; Hao, M., Franqui-Machin, R., Xu, H., Shaughnessy, J., Barlogie, B., Roodman, D., … Zhan, F. (2017). NEK2 induces osteoclast differentiation and bone destruction via heparanase in multiple myeloma. Leukemia, 31(7), 1648–1650. http://doi.org/10.1038/leu.2017.115Test; https://hdl.handle.net/1805/15895Test

الإتاحة: https://doi.org/10.1038/leu.2017.115Test
https://hdl.handle.net/1805/15895Test

المؤلفون: Hao, M, Franqui-Machin, R, Xu, H, Shaughnessy, J, Barlogie, B, Roodman, D, Quelle, D E, Janz, S, Tomasson, M H, Sanderson, R D, Qiu, L, Frech, I, Tricot, G, Zhan, F

المصدر: Leukemia ; volume 31, issue 7, page 1648-1650 ; ISSN 0887-6924 1476-5551

مصطلحات موضوعية: Oncology, Cancer Research, Hematology

الإتاحة: https://doi.org/10.1038/leu.2017.115Test
http://www.nature.com/articles/leu2017115.pdfTest
http://www.nature.com/articles/leu2017115Test

المؤلفون: Jung, O, Trapp-Stamborski, V, Purushothaman, A, Jin, H, Wang, H, Sanderson, R D, Rapraeger, A C

المصدر: Oncogenesis ; volume 5, issue 2, page e202-e202 ; ISSN 2157-9024

مصطلحات موضوعية: Cancer Research, Molecular Biology

الوصف: Multiple myeloma arises when malignant plasma cells invade and form multiple tumors in the bone marrow. High levels of heparanase (HPSE) correlate with poor prognosis in myeloma patients. A likely target of the enzyme is the heparan sulfate (HS) proteoglycan syndecan-1 (Sdc1, CD138), which is highly expressed on myeloma cells and contributes to poor prognosis in this disease. We find that HPSE promotes an invasive phenotype mediated by the very late antigen-4 (VLA-4, or α4β1 integrin) in myeloma cells plated on either fibronectin (FN) or vascular endothelial cell adhesion molecule-1 (VCAM-1), ligands that are prevalent in the bone marrow. The phenotype depends on vascular endothelial cell growth factor receptor-2 (VEGFR2), which is aberrantly expressed in myeloma, and is characterized by a highly protrusive lamellipodium and cell invasion. HPSE-mediated trimming of the HS on Sdc1 and subsequent matrix metalloproteinase-9-mediated shedding of the syndecan exposes a juxtamembrane site in Sdc1 that binds VEGFR2 and VLA-4, thereby coupling VEGFR2 to the integrin. Shed Sdc1 can be mimicked by recombinant Sdc1 ectodomain or by a peptide based on its binding motif, which causes VLA-4 to re-orient from the lagging edge (uropod) to the leading edge of migrating cells, couple with and activate VEGFR2. Peptides (called 'synstatins') containing only the VLA-4 or VEGFR2 binding sites competitively inhibit invasion, as they block coupling of the receptors. This mechanism is also utilized by vascular endothelial cells, in which it is also activated by HPSE, during endothelial cell tube formation. Collectively, our findings reveal for the first time the mechanism through which HPSE modulates Sdc1 function to promote both tumor cell invasion and angiogenesis, thereby driving multiple myeloma progression. The inhibitory synstatins, or inhibitors of HPSE enzyme activity, are likely to show promise as therapeutics against myeloma extravasation and spread.

الإتاحة: https://doi.org/10.1038/oncsis.2016.5Test
https://www.nature.com/articles/oncsis20165.pdfTest
https://www.nature.com/articles/oncsis20165Test

المؤلفون: Bernfield, M., Sanderson, R. D.

المصدر: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences, 1990 Mar 01. 327(1239), 171-186.

الوصول الحر: https://www.jstor.org/stable/55302Test

المؤلفون: Martari, M, Sanderson, R D

المصدر: South African Journal of Chemistry; Vol 61 (2008); 47-52

مصطلحات موضوعية: Peptide aggregation, self-assembly, critical self-assembly concentration, fluorescence, pyrene

الوصف: The study of the self-assembly properties of peptides and proteins is important for the understanding of molecular recognition processes and for the rational design of functional biomaterials. Novel bolaamphiphilic peptides and peptide hybrids incorporating non-natural aminoacids were designed around a model lysine/leucine-rich peptide with the intention to study their selfassembly behaviour. Steady-state fluorescence measurements using pyrene as fluorescent probe were adapted to the determination of the critical self-assembly concentrations (CSACs) of these amphiphilic peptides and peptide hybrids. Different experimental conditions were studied. The morphology of the peptide aggregates was evaluated by scanning electron microscopy (SEM). Concentration andpHhave been revealed to play a key role in the control of the process. Peptides presented different three-dimensional supramolecular arrangements that were correlated with their aminoacid compositions (specifically considering the presence of tyrosine and proline) and CSAC values.Keywords: Peptide aggregation, self-assembly, critical self-assembly concentration, fluorescence, pyrenePDF and Supplementry file attached

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=78975075580c::bd74d6118c2573e26c3f461858bd820cTest
https://www.ajol.info/index.php/sajc/article/view/123088Test

المؤلفون: Zengeni, E., Hartmann, P. C., Sanderson, R. D., Mallon, P. E.

المصدر: Journal of Applied Polymer Science ; volume 119, issue 2, page 1060-1066 ; ISSN 0021-8995 1097-4628

الوصف: A series of poly(acrylonitrile‐co‐methyl acrylate) copolymers of differing copolymer compositions are synthesized through free radical emulsion copolymerisation of methyl acrylate (MA) and acrylonitrile (AN) and by variation of the monomer feed ratios. The copolymers are characterized by NMR, SEC, WAXD, DMA, and positron annihilation lifetime spectroscopy. Results show that, there is a linear decrease in the glass transition temperature ( T g ) of the copolymers and the MA content. There is also a progressive decrease in the crystallinity of the copolymers with increasing MA content. Positron results show a sigmoidal variation in the ortho positronium lifetime with an increasing MA content. The low MA content, semi‐crystalline copolymers show a positive deviation from the linear additive relationship between the o‐Ps lifetime and MA content, whereas the higher MA content amorphous copolymers show a negative deviation from the linear additive behavior. The o‐PS intensity shows a linear behavior with MA content with a slight deviation from the additive linear behavior for the very high‐content MA copolymer. These variations in the measured positron lifetime parameters are interpreted in terms of the copolymer morphology. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011

الإتاحة: https://doi.org/10.1002/app.32550Test

المؤلفون: Vosloo, J. J., van Zyl, A. J. P., Nicholson, T. M., Sanderson, R. D., Gilbert, R. G.

المساهمون: S. Z. D. Cheng

مصطلحات موضوعية: Polymer Science, viscoelastic properties, branching, RAFT, Fragmentation Chain Transfer, Transfer Radical Polymerization, Size-exclusion Chromatography, Light-scattering Detector, Linear Rheology, Graft-polymerization, Branched Polymers, Elution Behavior, Raft Process, Side-chains, 250503 Characterisation of Macromolecules, 291401 Polymers, C1, 670705 Plastic products (incl. construction materials)

الوصف: A range of comb polymers of poly(n-butyl methacrylate), where the degree of polymerization (DP) of both the backbone and branches was controlled using RAFT-mediated free-radical polymerization, was synthesized using the method of Vosloo et al. [Macromolecules 2004;37: 2371]. Individual architectural parameters (branch length, branch number and DP of the comb backbone) of these relatively monodisperse samples were systematically varied in order to study the impact of each structural parameter on the thermal and theological properties of the resulting comb polymers. Differential scanning calorimetry showed lower glass transition temperatures for the comb polymers compared to the original linear backbones. There were negligible differences in glass transition temperatures between comb polymers containing branches of different lengths, and between comb polymers containing backbones of narrow and of broad molecular weight distributions. These observations suggest that because the comb polymers are very tightly spaced, the branches act in the same way as long chain polyBMA. Viscoelastic properties of the comb polymers were investigated using dynamic mechanical analysis, using time-temperature superposition to extend the rheological data over a wide frequency range. Major differences in the viscoelastic responses of the original linear backbones and the comb polymers were observed, which were explained in terms of arm retraction/relaxation leading to tube dilation. All comb polymers showed viscoelastic responses that are characteristic of combs, but differences in responses due to changes in branch length and branch number were difficult to detect. This was mainly due to the relatively high number of branches, whose retractions occurred over a broad frequency range, and thus dominated the observed changes in moduli, thereby possibly masking subtle differences in responses. (c) 2006 Elsevier Ltd. All rights reserved.

العلاقة: orcid:0000-0001-6988-114X

الإتاحة: https://doi.org/10.1016/j.polymer.2006.11.024Test
https://espace.library.uq.edu.au/view/UQ:128392Test

المؤلفون: Bowes, A., Mcleary, J. B., Sanderson, R. D.

المصدر: Journal of Polymer Science Part A: Polymer Chemistry ; volume 45, issue 4, page 588-604 ; ISSN 0887-624X 1099-0518

الوصف: Two trithiocarbonate reversible addition fragmentation chain transfer (RAFT) agents are compared in miniemulsion polymerization of styrene and butyl acrylate and the formation of seeded emulsion block copolymers. The order of block synthesis and the number of block segments per polymer are discussed. The use of nonionic surfactants is examined and the type of surfactant in relation to the monomer used is found to have a significant affect on latex formation. Conditions are shown by which AB and ABA type block copolymers can be successfully prepared via a seeded RAFT‐mediated emulsion polymerization. © 2006 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 45: 588–604, 2007

الإتاحة: https://doi.org/10.1002/pola.21827Test

المؤلفون: Fleet, Reda, McLeary, J. B., Grumel, V., Weber, W. G., Matahwa, H., Sanderson, R. D.

المصدر: Macromolecular Symposia ; volume 255, issue 1, page 8-19 ; ISSN 1022-1360 1521-3900

الوصف: Summary: In this study five xanthate (Reversible Addition‐Fragmentation chain Transfer (RAFT)/Macromolecular Design through Interchange of Xanthates (MADIX)) agents were synthesized, namely monofunctional, difunctional, trifunctional and tetrafunctional species of the form SC(O‐Z)‐S‐R, with different leaving groups and different activating moieties some of which are completely novel. Polyvinyl acetates (PVAc) in the form of linear, three armed and four armed star shaped polymers were then successfully synthesized in reactions mediated by these xanthate RAFT/MADIX agents.

الإتاحة: https://doi.org/10.1002/masy.200750902Test

المؤلفون: Ramiah, V., Matahwa, H., Weber, W., McLeary, J. B., Sanderson, R. D.

المصدر: Macromolecular Symposia ; volume 255, issue 1, page 70-80 ; ISSN 1022-1360 1521-3900

الوصف: Summary: Interesting new CMC and phase separation data of carbohydrate‐based self‐assembling core‐shell nanoparticles which were synthesized via the Reversible Addition‐Fragmentation Transfer (RAFT) process. The macro‐RAFT agent, poly(3‐O‐ Methacryloyl‐1,2:5,6‐di‐O‐isopropylidene‐D‐glucofuranose) (PMAlpGlc), was prepared by RAFT polymerization of the glycomonomer with cumyl phenyl dithioacetate as the chain transfer agent. Chain extension with styrene and methyl acrylate afforded the diblock copolymers (PMAlpGlc‐b‐styrene and PMAlpGlc‐b‐methyl acrylate) having predetermined molecular weight and narrow molecular weight distributions. Acidolysis of these diblock copolymers were undertaken and confirmed by NMR. Core‐shell nanoparticles were observed by TEM.

الإتاحة: https://doi.org/10.1002/masy.200750908Test