المؤلفون: Kazuto Kobayashi, Naohiro Hozumi, Sachiko Yoshida, Yuki Kawaguchi

المصدر: Proceedings of Symposium on Ultrasonic Electronics. 2023, 44:24

المؤلفون: Daiki Yamanaka, Kazuto Kobayashi, Maki Shibata, Naohiro Hozumi, Sachiko Yoshida, Yuki kawaguchi

المصدر: Proceedings of Symposium on Ultrasonic Electronics. 2023, 44:113

المؤلفون: Kazuki Tamura, Kazuyo Ito, Sachiko Yoshida, Jonathan Mamou, Katsutoshi Miura, Seiji Yamamoto

المصدر: Frontiers in Physics, Vol 11 (2023)

مصطلحات موضوعية: speed of sound, chemical fixation, frozen section, brain, glioma, Physics, QC1-999

الوصف: The elasticity of biological tissues is one of the physical characteristics of tissues and has attracted attention as a clinical diagnostic parameter. The elasticity can be determined on the microscopic scale with speed of sound (SoS) measurements using acoustic microscopy. In SoS measurements, a thin-sliced section is attached to a glass slide in the same manner as a light microscopic specimen. There are two main methods for preparing thin sections: paraffin-embedding and frozen-section. The frozen-section method requires fewer processing steps from sectioning to measurement and is considered to reduce artifacts in the sample compared with the paraffin-embedding method. Both methods need fixatives to keep tissue structures. Many reports of measurements using frozen sections are focused on soft tissues with relatively high protein contents. In this study, we determined the SoS in thin sections of four types of organs (brain, heart, liver, and kidney) prepared using two different methods (paraffin-embedding and frozen-section) and four different chemical fixatives (formalin, Karnovsky fixative (KF) 0.5% and 2.0% glutaraldehyde, and ethanol). The SoS in heart and liver samples prepared using KF showed good agreement with reported values for raw samples. For samples fixed with KF, the SoS increased as the glutaraldehyde concentration increased from 0.5% to 2.0%. A brain tumor sample was processed with KF 0.5%, and the SoS in the tumor was significantly higher than that in the non-tumor area. The results confirmed that it is possible to measure the SoS in brain samples with low protein contents using appropriate fixatives.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fphy.2023.1060296/fullTest; https://doaj.org/toc/2296-424XTest

الوصول الحر: https://doaj.org/article/fcfce58d75fc4ad1ab2e5d63a0114b38Test

المؤلفون: Fatini Athirah Mohmad Fadzeli, Kazuto Kobayashi, Mai Murakami, Naohiro Hozumi, Sachiko Yoshida, Tomohiro Kawashima, Yoshinobu Murakami, Yuki Kawaguchi, Yuto Isobe

المصدر: Proceedings of Symposium on Ultrasonic Electronics. 2022, 43:131

المؤلفون: Naoto Saito, Takeshi Hatanaka, Sachi Nakano, Yoichi Hazama, Sachiko Yoshida, Yoko Hachisu, Yoshiki Tanaka, Teruo Yoshinaga, Kenji Kashiwabara, Norio Kubo, Yasuo Hosouchi, Hiroki Tojima, Satoru Kakizaki, Toshio Uraoka

المصدر: Clinical Case Reports, Vol 10, Iss 7, Pp n/a-n/a (2022)

مصطلحات موضوعية: anti‐programmed death ligand‐1, hepatic resection, immune checkpoint inhibitor, liver cancer, vascular endothelial growth factor, Medicine, Medicine (General), R5-920

الوصف: Abstract An 81‐year‐old man initially underwent right hepatic lobectomy for liver cancer and was pathologically diagnosed with combined hepatocellular and cholangiocarcinoma (CHC). At 13 months after resection, multiple lymph node metastases were observed. We started atezolizumab plus bevacizumab (Atez/Bev), achieving a 7.5‐month progression‐free survival. Atez/Bev might exhibit efficacy for CHC patients.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2050-0904Test

الوصول الحر: https://doaj.org/article/2cd1c635ef1d44faa870f54f525fdf8dTest

المؤلفون: Atsushi Naganuma, Hiroaki Okamoto, Kazumoto Murata, Kenji Kashiwabara, Masaharu Takahashi, Naoto Saito, Sachi Nakano, Sachiko Yoshida, Satoru Kakizaki, Shigeo Nagashima, Takeshi Hatanaka, Takuya Kaburagi, Teruo Yoshinaga, Toshio Uraoka, Tsutomu Nishizawa, Yoichi Hazama, Yoko Hachisu, Yoshiki Tanaka, Yuichi Yamazaki

المصدر: Internal Medicine. 2022, 61(10):1537

المؤلفون: Takeshi Hatanaka, Yutaka Yata, Naoto Saito, Sachi Nakano, Yuya Nakano, Yoichi Hazama, Sachiko Yoshida, Yoko Hachisu, Yoshiki Tanaka, Teruo Yoshinaga, Atsushi Naganuma, Satoru Kakizaki

المصدر: Cancers, Vol 15, Iss 4, p 1289 (2023)

مصطلحات موضوعية: overweight, hepatocellular carcinoma, radiofrequency ablation, microwave ablation, body mass index, technical success rate, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: This study aimed to investigate the effect of being overweight on the outcome of ablation therapy for patients with early-stage hepatocellular carcinoma (HCC). This retrospective study included 198 patients with HCC who underwent radiofrequency ablation or microwave ablation at Gunma Saiseikai Maebashi Hospital between April 2017 and December 2021. We divided the patients into two groups based on their body mass index (BMI): overweight (BMI ≥ 25 kg/m2, n = 74 (37.4%)) and non-overweight (BMI < 25 kg/m2, n = 124 (62.6%)). The technical success rates (TSRs) in the first session were 78.4% and 90.3% in overweight and non-overweight patients, respectively, with a significant difference (p = 0.03). Additional ablation therapy for residual tumors was required in 15 (20.3%) overweight and 11 (8.9%) non-overweight patients (p = 0.03), resulting in 95.9% and 99.2% TSRs at the final session, respectively, without a significant difference (p = 0.3). While local tumor progression and distant recurrence rates were not significantly different between the two groups, overall survival was better in overweight patients than in non-overweight patients (p < 0.001). Despite the potential adverse impact of being overweight on public health problems, the present findings showed the relationship between being overweight and improved survival. The negative aspects of being overweight might remain as minor technical issues in HCC patients receiving ablation therapy.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2072-6694/15/4/1289Test; https://doaj.org/toc/2072-6694Test

الوصول الحر: https://doaj.org/article/3792a2139b0149c2b71ae637433dd38bTest

المؤلفون: Sachiko Yoshida, Kazuo Asanoma, Hiroshi Yagi, Ichiro Onoyama, Emiko Hori, Yumiko Matsumura, Kaoru Okugawa, Hideaki Yahata, Kiyoko Kato

المصدر: BMC Cancer, Vol 21, Iss 1, Pp 1-12 (2021)

مصطلحات موضوعية: Cancer-associated fibroblasts, Endometrial neoplasms, Extracellular matrix, FN1, SPARC, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Abstract Background Matricellular glycoprotein, SPARC is a secreted molecule, that mediates the interaction between cells and extracellular matrix. SPARC functions as a regulator of matrix organization and modulates cell behavior. In various kinds of cancer, strong SPARC expression was observed in stromal tissues as well as in cancer epithelial cells. The function of SPARC in cancer cells is somewhat controversial and its impact on peritumoral stromal cells remains to be resolved. Methods We investigated the effects of SPARC expression in endometrial cancer cells on the surrounding stromal fibroblasts using in vitro co-culture system. Changes in characteristics of fibroblasts were examined by analysis of fibroblast-specific markers and in vitro contraction assay. Results SPARC induced AKT phosphorylation and epithelial-to-mesenchymal transition, consistent with previous reports. Cancer-associated fibroblasts of endometrial cancer expressed higher levels of mesenchymal- and fibroblast-associated factors and had a stronger contraction ability. Unexpectedly, cancer-associated fibroblasts expressed comparable levels of SPARC compared with fibroblasts from normal endometrium. However, co-culture of normal fibroblasts with SPARC-expressing Ishikawa cells resulted in activation of the fibroblasts. Immunodepletion of SPARC did not affect the activation of fibroblasts. Conclusions Our data indicated that SPARC activated fibroblasts only in the presence of fibronectin, which was abundantly secreted from SPARC-expressing endometrial cancer cells. These results suggested that a SPARC-fibronectin-mediated activation of fibroblasts might be involved in enhanced mobility and invasion of cancer cells.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2407Test

الوصول الحر: https://doaj.org/article/98385d5b97114b748933cb97685790d7Test

المؤلفون: Izumi Suzuki, Sachiko Yoshida, Kouichi Tabu, Soshi Kusunoki, Yumiko Matsumura, Hiroto Izumi, Kazuo Asanoma, Hiroshi Yagi, Ichiro Onoyama, Kenzo Sonoda, Kimitoshi Kohno, Tetsuya Taga, Atsuo Itakura, Satoru Takeda, Kiyoko Kato

المصدر: Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)

مصطلحات موضوعية: Medicine, Science

الوصف: Abstract Y-box binding protein 2 (YBX2) has been associated with the properties of both germ cells and cancer cells. We hypothesized that YBX2 might contribute to the characteristics of cancer stem cells (CSCs). In this study, we clarified the function of YBX2 in endometrial cancer stem cells. We established a human YBX2-expressing Ishikawa (IK) cell line (IK-YBX2 cells). We analyzed gene expression associated with stemness and isolated SP cells from IK-YBX2 cells. The SP population of IK-YBX2 cells, the expression of ALDH1 and serial sphere-forming capacity were associated with levels of YBX2 expression. IK-YBX2 cells were resistant to anti-cancer drugs. In gene expression analysis, a gene for cancer testis antigen, CT45, was generally overexpressed in IK-YBX2 cells. YBX2-mediated CT45 expression was associated with increased levels of self-renewal capacity and paclitaxel resistance. The level of CT45 expression was enhanced in high-grade and/or advanced stages of human endometrial cancer tissues. We conclude that expression of YBX2 is essential for the stem cell-like phenotype. CT45 contributes to stemness associated with YBX2 and might be related to the progression of endometrial cancer.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/2393a0674b334285888ea55f9ddad4b0Test

المؤلفون: Hideki Kumagai, Kazuto Kobayashi, Sachiko Yoshida, Koji Yokoyama, Norio Hirota, Takanori Yamagata

المصدر: Acoustics, Vol 3, Iss 1, Pp 3-10 (2020)

مصطلحات موضوعية: liver fibrosis, fatty liver, ultrasonics, tissue characterization, speed of sound, Physics, QC1-999

الوصف: Scanning acoustic microscopy reveals information on histology and acoustic impedance through tissues. The objective of the present study was to investigate whether acoustic impedance values in the liver over time reflect the progression of steatohepatitis through different grades and stages, and whether this approach can visualize histologic features of the disease. Mice were divided into two groups: a control group and a steatohepatitis group prepared by keeping the mice on a methionine and choline-deficient diet for 56 weeks. The hepatic lobe was excised for measurement of impedance and observation of microscopic structure using a commercially available scanning acoustic microscopy system with a central frequency of 320 MHz. Scanning acoustic microscopy revealed that acoustic impedance through liver tissue with steatohepatitis temporarily decreased with the degree of fat deposition and then increased in parallel with the progression of inflammation and fibrosis. However, the acoustic images obtained did not allow discrimination of detailed microstructures from those seen using light microscopy. In conclusion, estimation of acoustic impedance appears to have potential clinical applications, such as for monitoring or follow-up studies.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2624-599X/3/1/2Test; https://doaj.org/toc/2624-599XTest

الوصول الحر: https://doaj.org/article/8bc64a77e24d4e4f9610f6385bcfdb9eTest