المؤلفون: Haddad, Emna, Bottet, Benjamin, Thiebaut, Pierre-Alain, Morin, Samira, Dreyfus, Hélène, Vannier, Élise, Vincent, Colette, Marguet, Florent, Lamy, Aude, Sobol, Hagay, Baste, Jean-Marc, Guisier, Florian, Sabourin, Jean-Christophe, Piton, Nicolas

المصدر: JTO Clinical and Research Reports ; volume 5, issue 1, page 100595 ; ISSN 2666-3643

مصطلحات موضوعية: Pulmonary and Respiratory Medicine, Oncology

الإتاحة: https://doi.org/10.1016/j.jtocrr.2023.100595Test
https://api.elsevier.com/content/article/PII:S2666364323001388?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2666364323001388?httpAccept=text/plainTest

المؤلفون: Arhant, Gwenaëlle, Lachkar, Samy, Thiebaut, Pierre-Alain, Marguet, Florent, Lamy, Aude, Thiberville, Luc, Salaün, Mathieu, Guisier, Florian, Sabourin, Jean-Christophe, Piton, Nicolas

المساهمون: Boehringer Ingelheim France SAS, Boehringer Ingelheim

المصدر: JTO Clinical and Research Reports ; volume 5, issue 2, page 100596 ; ISSN 2666-3643

مصطلحات موضوعية: Pulmonary and Respiratory Medicine, Oncology

الإتاحة: https://doi.org/10.1016/j.jtocrr.2023.100596Test
https://api.elsevier.com/content/article/PII:S266636432300139X?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S266636432300139X?httpAccept=text/plainTest

المؤلفون: Mouaddine, Amal, Thiebaut, Pierre-Alain, Sabourin, Jean-Christophe

المصدر: Annales de Pathologie ; volume 44, issue 2, page 137-141 ; ISSN 0242-6498

مصطلحات موضوعية: Pathology and Forensic Medicine

الإتاحة: https://doi.org/10.1016/j.annpat.2023.11.001Test
https://api.elsevier.com/content/article/PII:S0242649823002274?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0242649823002274?httpAccept=text/plainTest

المؤلفون: Lachkar, Samy, Faur, Quentin, Marguet, Florent, Veresezan, Liana, Bubenheim, Michael, Salaün, Mathieu, Thiberville, Luc, Sabourin, Jean‐Christophe, Guisier, Florian, Piton, Nicolas

المصدر: Thoracic Cancer ; volume 14, issue 22, page 2149-2157 ; ISSN 1759-7706 1759-7714

الوصف: Background Endobronchial ultrasound‐guided (EBUS) transbronchial needle aspiration (TBNA) has significantly improved the diagnostic workup for intrathoracic lymphadenopathies. More recently, EBUS intranodal forceps biopsy (IFB) has been developed in an attempt to maximize diagnostic yield by providing additional tissue. In this study, we aimed to assess the improvement of diagnostic yield with EBUS‐TBNA combined with EBUS‐IFB, compared to EBUS‐TBNA alone. Methods Consecutive patients who had 19‐G EBUS‐TBNA and EBUS‐IFB from August 30, 2018, to September 28, 2021, were included. Four senior pathologists retrospectively analyzed, independently and blindly, first, only the EBUS‐TBNA samples (cell block), then, at least 1 month later, both samples from EBUS‐TBNA and from EBUS‐IFB together. Results Fifty patients were included in the study and 52 lymph nodes were analyzed. Diagnostic yield was 77% (40/52) for EBUS‐TBNA alone and 94% (49/52) when combined with EBUS‐IFB ( p = 0.023). Malignancy was diagnosed with EBUS‐TBNA combined with EBUS‐IFB in 25/26 cases (96%), versus 22/26 (85%) with EBUS‐TBNA alone ( p = 0.35); and 4/5 (80%) versus 2/5 (40%) for lymphoma specifically. Kappa interobserver agreement was 0.92 for EBUS‐IFB and 0.87 for EBUS‐TBNA alone. Nonmalignant condition was diagnosed with EBUS‐TBNA combined with EBUS‐IFB in 24/26 cases (92%), versus 18/26 (69%) for EBUS‐TBNA alone ( p = 0.07). Conclusion The use of EBUS‐IFB combined with 19‐G EBUS‐TBNA improves the mediastinal lymph node diagnostic yield However the benefit appears to be mainly restricted to nonmalignant histology.

الإتاحة: https://doi.org/10.1111/1759-7714.15000Test

المؤلفون: Neuzillet, Cindy, Artru, Pascal, Assenat, Eric, Edeline, Julien, Adhoute, Xavier, Sabourin, Jean-Christophe, Turpin, Anthony, Coriat, Romain, Malka, David

المساهمون: Servier

المصدر: Targeted Oncology ; volume 18, issue 1, page 51-76 ; ISSN 1776-2596 1776-260X

مصطلحات موضوعية: Pharmacology (medical), Cancer Research, Oncology

الإتاحة: https://doi.org/10.1007/s11523-022-00942-6Test

المؤلفون: Hofman, Véronique, Heeke, Simon, Bontoux, Christophe, Chalabreysse, Lara, Barritault, Marc, Bringuier, Pierre Paul, Fenouil, Tanguy, Benzerdjeb, Nazim, Begueret, Hugues, Merlio, Jean Philippe, Caumont, Charline, Piton, Nicolas, Sabourin, Jean-Christophe, Evrard, Solène, Syrykh, Charlotte, Vigier, Anna, Brousset, Pierre, Mazieres, Julien, Long-Mira, Elodie, Benzaquen, Jonathan, Boutros, Jacques, Allegra, Maryline, Tanga, Virginie, Lespinet-Fabre, Virginie, Salah, Myriam, Bonnetaud, Christelle, Bordone, Olivier, Lassalle, Sandra, Marquette, Charles-Hugo, Ilié, Marius, Hofman, Paul

المصدر: JTO Clinical and Research Reports ; volume 4, issue 2, page 100457 ; ISSN 2666-3643

مصطلحات موضوعية: Pulmonary and Respiratory Medicine, Oncology

الإتاحة: https://doi.org/10.1016/j.jtocrr.2022.100457Test
https://api.elsevier.com/content/article/PII:S2666364322001813?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2666364322001813?httpAccept=text/plainTest

المؤلفون: Mintet, Elodie, Lavigne, Jérémy, Paget, Vincent, Tarlet, Georges, Buard, Valérie, Guipaud, Olivier, Sabourin, Jean-Christophe, Iruela-Arispe, Maria-Luisa, Milliat, Fabien, François, Agnès

المصدر: Scientific reports. 7(1)

مصطلحات موضوعية: Cells, Cultured, Animals, Humans, Mice, Proctitis, Radiation Injuries, Repressor Proteins, Fluorescent Antibody Technique, Immunohistochemistry, Gene Deletion, Phenotype, Basic Helix-Loop-Helix Transcription Factors, Epithelial-Mesenchymal Transition, Human Umbilical Vein Endothelial Cells, Transcriptome, Biomarkers, Stem Cell Research, Prevention, Digestive Diseases, 2.1 Biological and endogenous factors, Biochemistry and Cell Biology, Other Physical Sciences

الوصف: The current study evaluated the role of Hey2 transcription factor in radiation-induced endothelial-to-mesenchymal transition (EndoMT) and its impact on radiation-induced tissue damage in mice. Phenotypic modifications of irradiated, Hey2 siRNA- and Hey2 vector plasmid-transfected human umbilical vein endothelial cells (HUVECs) resembling EndoMT were monitored by qPCR, immunocytochemistry and western blots. Subsequently, in mice, a Cre-LoxP strategy for inactivation of Hey2 specifically in the endothelium was used to study the biological consequences. Total body irradiation and radiation proctitis were monitored to investigate the impact of conditional Hey2 deletion on intestinal stem cells and microvascular compartment radiosensitivity, EndoMT and rectal damage severity. We found that EndoMT occurs in irradiated HUVECs with concomitant Hey2 mRNA and protein increase. While Hey2 silencing has no effect on radiation-induced EndoMT in vitro, Hey2 overexpression is sufficient to induce phenotypic conversion of endothelial cells. In mice, the conditional deletion of Hey2 reduces EndoMT frequency and the severity of rectal tissue damage. Our data indicate that the reduction in mucosal damage occurs through decline in stem/clonogenic epithelial cell loss mediated by microvascular protection. EndoMT is involved in radiation proctitis and this study demonstrates that a strategy based on the reduction of EndoMT mitigates intestinal tissue damage.

الوصول الحر: https://escholarship.org/uc/item/02s5d5kpTest

المؤلفون: Guérin, René, Menard, Anne-Lise, Angot, Emilie, Piton, Nicolas, Vera, Pierre, Schwarz, Lilian, Sabourin, Jean-Christophe, Laé, Marick, Thiébaut, Pierre-Alain

المصدر: Diagnostic Pathology; 4/20/2024, Vol. 19 Issue 1, p1-7, 7p

مصطلحات موضوعية: LITERATURE reviews, SARCOMA, MOLECULAR biology, TUMORS

مستخلص: Background: Alveolar soft part sarcoma is a rare tumour of soft tissues, mostly localized in muscles or deep soft tissues of the extremities. In rare occasions, this tumour develops in deep tissues of the abdomen or pelvis. Case presentation: In this case report, we described the case of a 46 year old man who developed a primary splenic alveolar soft part sarcoma. The tumour displayed typical morphological alveolar aspect, as well as immunohistochemical profile notably TFE3 nuclear staining. Detection of ASPSCR1 Exon 7::TFE3 Exon 6 fusion transcript in molecular biology and TFE3 rearrangement in FISH confirmed the diagnosis. Conclusion: We described the first case of primary splenic alveolar soft part sarcoma, which questions once again the cell of origin of this rare tumour. [ABSTRACT FROM AUTHOR]

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المؤلفون: Hofman, Véronique, Heeke, Simon, Bontoux, Christophe, Chalabreysse, Lara, Barritault, Marc, Bringuier, Pierre, Paul, Fenouil, Tanguy, Benzerdjeb, Nazim, Begueret, Hugues, Merlio, Jean, Philippe, Caumont, Charline, Piton, Nicolas, Sabourin, Jean-Christophe, Evrard, Solène, Syrykh, Charlotte, Vigier, Anna, Brousset, Pierre, Mazieres, Julien, Long-Mira, Elodie, Benzaquen, Jonathan, Boutros, Jacques, Allegra, Maryline, Tanga, Virginie, Lespinet-Fabre, Virginie, Salah, Myriam, Bonnetaud, Christelle, Bordone, Olivier, Lassalle, Sandra, Marquette, Charles-Hugo, Ilié, Marius, Hofman, Paul

المساهمون: Institut de Recherche sur le Cancer et le Vieillissement (IRCAN), Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UniCA), Hospices Civils de Lyon (HCL), Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard Lyon -Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Génomique et Médecine Personnalisée du Cancer et des Maladies Neuropsychiatriques (GPMCND), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)

المصدر: ISSN: 2666-3643.

مصطلحات موضوعية: Gene fusion, Non-small cell lung carcinoma, Next generation sequencing, RT-PCR, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract

الوصف: International audience ; Introduction: Gene fusion testing of ALK, ROS1, RET, NTRK, and MET exon 14 skipping mutations is guideline recommended in nonsquamous NSCLC (NS-NSCLC). Nevertheless, assessment is often hindered by the limited availability of tissue and prolonged next-generation sequencing (NGS) testing, which can protract the initiation of a targeted therapy. Therefore, the development of faster gene fusion assessment is critical for optimal clinical decision-making. Here, we compared two ultrafast gene fusion assays (UFGFAs) using NGS (Genexus, Oncomine Precision Assay, Thermo Fisher Scientific) and a multiplex reverse-transcriptase polymerase chain reaction (Idylla, GeneFusion Assay, Biocartis) approach at diagnosis in a retrospective series of 195 NS-NSCLC cases and five extrapulmonary tumors with a known NTRK fusion.Methods: A total of 195 NS-NSCLC cases (113 known gene fusions and 82 wild-type tumors) were included retrospectively. To validate the detection of a NTRK fusion, we added five NTRK-positive extrathoracic tumors. The diagnostic performance of the two UFGFAs and standard procedures was compared.Results: The accuracy was 92.3% and 93.1% for Idylla and Genexus, respectively. Both systems improved the sensitivity for detection by including a 5′-3′ imbalance analysis. Although detection of ROS1, MET exon 14 skipping, and RET was excellent with both systems, ALK fusion detection was reduced with sensitivities of 87% and 88%, respectively. Idylla had a limited sensitivity of 67% for NTRK fusions, in which only an imbalance assessment was used.Conclusions: UFGFA using NGS and reverse-transcriptase polymerase chain reaction approaches had an equal level of detection of gene fusion but with some technique-specific limitations. Nevertheless, UFGFA detection in routine clinical care is feasible with both systems allowing faster initiation of therapy and a broad degree of screening.

العلاقة: hal-04490508; https://hal.science/hal-04490508Test; https://hal.science/hal-04490508/documentTest; https://hal.science/hal-04490508/file/main%20%282%29.pdfTest

الإتاحة: https://doi.org/10.1016/j.jtocrr.2022.100457Test
https://hal.science/hal-04490508Test
https://hal.science/hal-04490508/documentTest
https://hal.science/hal-04490508/file/main%20%282%29.pdfTest

المؤلفون: Fortier, Alexa, Mokadem, Seif, Cornet, Emlyn, Cornu, Jean-Nicolas, Sabourin, Jean-Christophe, Pfister, Christian

المصدر: Urology Case Reports ; volume 43, page 102055 ; ISSN 2214-4420

مصطلحات موضوعية: Urology

الإتاحة: https://doi.org/10.1016/j.eucr.2022.102055Test
https://api.elsevier.com/content/article/PII:S2214442022000675?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2214442022000675?httpAccept=text/plainTest