المؤلفون: Rouzaire, Marion

مرشدي الرسالة: Clermont-Ferrand 1, Gallot, Denis

مصطلحات موضوعية: Membranes fœtales, Rétinoïdes, Structures de l'embryon, Fetal membranes, Retinoids, Embryonic structures

الوصف: La vitamine A et ses dérivés actifs (les rétinoïdes) sont des molécules essentielles à la vie et ce dès la période embryonnaire. Molécules de choix dans de nombreuses applications thérapeutiques, notamment dans les domaines de la dermatologie et de l’ophtalmologie, elles sont le plus couramment utilisées pour leurs propriétés pro-cicatrisantes. Malgré cette utilisation courante en clinique, les mécanismes cellulaires et moléculaires permettant aux rétinoïdes de promouvoir un processus de cicatrisation restent encore mal connus. Afin de mieux appréhender ces mécanismes, mais aussi afin d’élargir leur utilisation clinique à la rupture prématurée des membranes (RPM), je me suis intéressée aux propriétés pro-cicatrisantes de l’atRA (l’un des dérivés actifs de la vitamine A) sur les membranes fœtales. Alors que l’amnios et le chorion sont physiologiquement incapables d’initier un processus de cicatrisation suite à une lésion, nos résultats démontrent un effet positif de l’atRA sur la migration des cellules épithéliales amniotiques (amniocytes primaires), conduisant ainsi à une potentialisation de la cicatrisation de plus de 80% in vitro. Ce travail, complété par une analyse transcriptomique réalisée sur des membranes fœtales et des amniocytes primaires traités ou non à l’atRA, a permis d’identifier de nombreux gènes régulés par l’atRA au sein de l’amnios et des amniocytes. Parmi ces gènes, je me suis intéressée à un membre de la famille des lysyl oxydases, LOXL4, qui joue un rôle clé dans la dynamique de la matrice extracellulaire en régulant la réticulation du collagène. Une étude de régulation transcriptionnelle associée à de la promotologie ont tout d’abord permis de montrer que l’atRA induisait l’expression du gène LOXL4 de manière directe. Puis, l’inhibition de la protéine LOXL4 lors de tests de blessure (par le β-aminopropionitrile et par un siARN spécifique) nous a permis de démontrer que l’effet pro-cicatrisant de l’atRA était médié, au moins en partie, par la rétino-induction deLOXL4. Ces nouveaux éléments apportés par ce travail dans la compréhension des mécanismes moléculaires utilisés par l’atRA afin de promouvoir la cicatrisation permettent d’envisager son utilisation future dans la prise en charge clinique des ruptures prématurées.
Vitamin A and its active derivates (retinoids) are essential molecules for life from the embryonic development to the adulthood. Because of these numerous functions and especially because of their pro-healing properties, they are commonly used in clinic in dermatology and ophthalmology in particular. Despite this routine clinical use, the molecular and cellular mechanisms used by retinoids to promote a healing process remain unclear. To better understand these mechanisms, but also to expand its clinical use to the premature rupture of fetal membranes (PROM), we looked to pro-healing properties of atRA (active derivative of vitamin A) on the human fetal membranes. While amnion and chorion are unable to initiate a physiological process of healing after injury, our results demonstrate a positive effect of atRA on the migration of primary amniocytes, leading to an healing improvement of up to 80% in vitro. This work, completed by a transcriptomic analysis performed on fetal membranes and primary amniocytes treated or not with atRA, allowed the identification of many genes regulated by atRA within the amnion and the amniocytes. Among these genes, we looked to a member of the lysyl oxidase family, LOXL4, which plays a key role in the dynamic of the extracellular matrix by regulating collagen crosslinking. First, transcription and promotology experiments have shown that atRA strongly induced the expression of LOXL4 in a direct manner. Then, the inhibition of the LOXL4 protein in scratch assay experiments (using β-aminopropionitrile or a specific siRNA) allowed us to demonstrate that the pro-healing effect of atRA was mediated, at least in part, by the retinoid-induction of this gene. Besides providing new elements to understand how atRA exerts their pro-healing properties, this work proposes atRA as a promising candidate to improve the clinical management of premature rupture of the fetal membranes by promoting re-epithelialization of the amnion.

الإتاحة: http://www.theses.fr/2016CLF1MM18/documentTest

المؤلفون: Puissegur, Amandine, Accoceberry, Marie, Rouzaire, Marion, Pereira, Bruno, Herault, Marie, Bruhat, Clément, Delabaere, Amélie, Gallot, Denis

المساهمون: Service Obstétrique CHU Clermont-Ferrand, CHU Estaing Clermont-Ferrand, CHU Clermont-Ferrand-CHU Clermont-Ferrand, Centre d'Investigation Clinique CHU Clermont-Ferrand (CIC 1405), Institut National de la Santé et de la Recherche Médicale (INSERM)-Direction de la recherche clinique et de l’innovation CHU Clermont-Ferrand (DRCI), Unité de Biostatistiques CHU Clermont-Ferrand, Direction de la recherche clinique et de l’innovation CHU Clermont-Ferrand (DRCI), Institut Pascal (IP), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)

المصدر: ISSN: 2077-0383 ; Journal of Clinical Medicine ; https://hal.science/hal-04350514Test ; Journal of Clinical Medicine, 2023, 12 (8), pp.3036. ⟨10.3390/jcm12083036⟩.

مصطلحات موضوعية: perineal tear, lacerations, case–control studies, risk factors, [SDV.MHEP.GEO]Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie

الوصف: International audience ; We conducted a retrospective case–control study in patients presenting a perineal tear (second degree or higher) or episiotomy complicated by wound breakdown during maternity stay to identify risk factors associated with wound breakdown in early postpartum with a view to improving the quality of care. We collected ante- and intrapartum characteristics and outcomes at the postpartum visit. In all, 84 cases and 249 control subjects were included. In univariate analysis, primiparity, absence of history of vaginal delivery, a longer second phase of labour, instrumental delivery, and a higher degree of laceration emerged as risk factors for early perineal suture breakdown postpartum. Gestational diabetes, peripartum fever, streptococcus B, and suture technique did not emerge as risk factors for perineal breakdown. Multivariate analysis confirmed that instrumental delivery (OR = 2.18 [1.07; 4.41], p = 0.03) and a longer second phase of labour (OR = 1.72 [1.23; 2.42], p = 0.001) were risk factors for early perineal suture breakdown.

العلاقة: hal-04350514; https://hal.science/hal-04350514Test; https://hal.science/hal-04350514/documentTest; https://hal.science/hal-04350514/file/jcm-12-03036.pdfTest

الإتاحة: https://doi.org/10.3390/jcm12083036Test
https://hal.science/hal-04350514Test
https://hal.science/hal-04350514/documentTest
https://hal.science/hal-04350514/file/jcm-12-03036.pdfTest

المؤلفون: Pambet, Mathilde, Sirodot, Fanny, Pereira, Bruno, Cahierc, Romain, Delabaere, Amélie, Comptour, Aurélie, Rouzaire, Marion, Sapin, Vincent, Gallot, Denis

المساهمون: Centre d'Investigation Clinique CHU Clermont-Ferrand (CIC 1405), Institut National de la Santé et de la Recherche Médicale (INSERM)-Direction de la recherche clinique et de l’innovation CHU Clermont-Ferrand (DRCI), CHU Clermont-Ferrand-CHU Clermont-Ferrand, Unité de Biostatistiques CHU Clermont-Ferrand, Direction de la recherche clinique et de l’innovation CHU Clermont-Ferrand (DRCI), Institut Pascal (IP), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Service Obstétrique CHU Clermont-Ferrand, CHU Estaing Clermont-Ferrand, Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), Service de Biochimie et Génétique Moléculaire CHU Clermont-Ferrand, CHU Gabriel Montpied Clermont-Ferrand, CHU Clermont-Ferrand-CHU Clermont-Ferrand-CHU Estaing Clermont-Ferrand, CHU Clermont-Ferrand

المصدر: ISSN: 2077-0383 ; Journal of Clinical Medicine ; https://hal.science/hal-04350517Test ; Journal of Clinical Medicine, 2023, 12 (17), pp.5707. ⟨10.3390/jcm12175707⟩.

مصطلحات موضوعية: preterm labor, IGFBP-1, IL-6, fibronectin, vaginal biomarkers, [SDV.MHEP.GEO]Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity

الوصف: International audience ; We conducted a prospective double-blind study to compare two vaginal diagnostic methods in singleton pregnancies with threatened preterm labor (TPL) at the University Hospital of Clermont-Ferrand (France) from August 2018 to December 2020. Our main objective was to compare the diagnostic capacity at admission, in terms of positive predictive value (PPV) and negative predictive value (NPV), of Premaquick® (combined detection of IL-6/total IGFBP-1/native IGFBP-1) and QuikCheck fFN™ (fetal fibronectin) for delivery within 7 days in cases of TPL. We included 193 patients. Premaquick® had a sensitivity close to 89%, equivalent to QuikCheck fFN™, but a higher statistical specificity of 49.5% against 38.6% for QuikCheck fFN™. We found no superiority of Premaquick® over QuickCheck fFN™ in terms of PPV (6.6% vs. 7.9%), with NPV being equivalent in predicting childbirth within 7 days in cases of TPL (98.6% vs. 98.9%). Nevertheless, the combination of positive native and total IGFBP-1 and the combination of all three positive markers were associated with a higher PPV. Our results, though non-significant, support this combined multiple-biomarker approach to improve testing in terms of predictive values.

العلاقة: hal-04350517; https://hal.science/hal-04350517Test; https://hal.science/hal-04350517/documentTest; https://hal.science/hal-04350517/file/jcm-12-05707-v2.pdfTest

الإتاحة: https://doi.org/10.3390/jcm12175707Test
https://hal.science/hal-04350517Test
https://hal.science/hal-04350517/documentTest
https://hal.science/hal-04350517/file/jcm-12-05707-v2.pdfTest

المؤلفون: Cahierc, Romain, Debost-Legrand, Anne, Lane, Sophie, Rouzaire, Marion, Bonnin, Martine, Delabaere, Amélie, Gallot, Denis

المصدر: Gynécologie Obstétrique Fertilité & Sénologie ; volume 51, issue 10, page 448-454 ; ISSN 2468-7189

مصطلحات موضوعية: Obstetrics and Gynecology, Reproductive Medicine

الإتاحة: https://doi.org/10.1016/j.gofs.2023.08.001Test
https://api.elsevier.com/content/article/PII:S2468718923001629?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2468718923001629?httpAccept=text/plainTest

المؤلفون: Reitz, Aurélie, Rouzaire, Marion, Cahierc, Romain, Pereira, Bruno, Lemal, Richard, Garrouste, Cyril, Gallot, Denis

المصدر: Gynécologie Obstétrique Fertilité & Sénologie ; ISSN 2468-7189

مصطلحات موضوعية: Obstetrics and Gynecology, Reproductive Medicine

الإتاحة: https://doi.org/10.1016/j.gofs.2024.01.011Test
https://api.elsevier.com/content/article/PII:S2468718924000345?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2468718924000345?httpAccept=text/plainTest

المؤلفون: Belville, Corinne, Ponelle-Chachuat, Flora, Rouzaire, Marion, Gross, Christelle, Pereira, Bruno, Gallot, Denis, Sapin, Vincent, Blanchon, Loïc

المساهمون: Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), Imagerie Moléculaire et Stratégies Théranostiques (IMoST), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA), CHU Clermont-Ferrand, CHU Gabriel Montpied Clermont-Ferrand

المصدر: EISSN: 2050-084X ; eLife ; https://hal.science/hal-03630274Test ; eLife, 2022, 11, pp.e71521. ⟨10.7554/eLife.71521⟩

مصطلحات موضوعية: [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology

الوصف: International audience ; The integrity of human fetal membranes is crucial for harmonious fetal development throughout pregnancy. Their premature rupture is often the consequence of a physiological phenomenon that has been exacerbated. Beyond all the implied biological processes, inflammation is of primary importance and is qualified as ‘sterile’ at the end of pregnancy. In this study, complementary methylomic and transcriptomic strategies on amnion and choriodecidua explants obtained from the altered (cervix zone) and intact fetal membranes at term and before labour were used. By cross-analysing genome-wide studies strengthened by in vitro experiments, we deciphered how the expression of toll-like receptor 4 (TLR4), an actor in pathological fetal membrane rupture, is controlled. Indeed, it is differentially regulated in the altered zone and between both layers by a dual mechanism: (1) the methylation of TLR4 and miRNA promoters and (2) targeting by miRNA (let-7a-2 and miR-125b-1) acting on the 3’-UTR of TLR4. Consequently, this study demonstrates that fine regulation of TLR4 is required for sterile inflammation establishment at the end of pregnancy and that it may be dysregulated in the pathological premature rupture of membranes.

العلاقة: hal-03630274; https://hal.science/hal-03630274Test; https://hal.science/hal-03630274/documentTest; https://hal.science/hal-03630274/file/Belville%20et%20al_Elife.pdfTest; PUBMEDCENTRAL: PMC8816379

الإتاحة: https://doi.org/10.7554/eLife.71521Test
https://hal.science/hal-03630274Test
https://hal.science/hal-03630274/documentTest
https://hal.science/hal-03630274/file/Belville%20et%20al_Elife.pdfTest

المؤلفون: Devillard, Eric, Petillon, Fanny, Rouzaire, Marion, Pereira, Bruno, Accoceberry, Marie, Houlle, Céline, Dejou-Bouillet, Lydie, Bouchet, Pamela, Delabaere, Amélie, Gallot, Denis

المساهمون: Service de Gynécologie CHU Clermont-Ferrand, CHU Estaing Clermont-Ferrand, CHU Clermont-Ferrand-CHU Clermont-Ferrand, Service Obstétrique CHU Clermont-Ferrand, Centre Hospitalier de Vienne Lucien Hussel (CH Vienne), Unité de Biostatistiques CHU Clermont-Ferrand, Direction de la recherche clinique et de l’innovation CHU Clermont-Ferrand (DRCI), Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)

المصدر: ISSN: 2077-0383 ; Journal of Clinical Medicine ; https://hal.science/hal-03824651Test ; Journal of Clinical Medicine, 2022, 11 (6), pp.1525. ⟨10.3390/jcm11061525⟩.

مصطلحات موضوعية: cervical ripening balloon, labor induction, nulliparous, premature rupture of membranes, unfavorable cervix, [SDV.MHEP.GEO]Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics

الوصف: International audience ; Background: The aim of this study is to demonstrate that a double balloon catheter combined with oxytocin decreases time between induction of labor and delivery (TID) as compared to a vaginal dinoprostone insert in cases of premature rupture of membranes at term. Methods: This is a prospective, randomized, controlled trial including patient undergoing labor induction for PROM at term with an unfavorable cervix in Clermont-Ferrand university hospital. We compared the double balloon catheter over a period of 12 h with adjunction of oxytocin 6 h after catheter insertion versus dinoprostone vaginal insert. After device ablation, cervical ripening continued only with oxytocin. The main outcome was TID. Secondary outcomes concerned delivery mode, as well as maternal and fetal outcome, and were adjusted for parity. Results: 40 patients per group were randomized. Each group had similar baseline characteristics. The study failed to demonstrate reduced TID (16.2 versus 20.2 h, ES = 0.16 (−0.27 to 0.60), p = 0.12) in the catheter group versus dinoprostone except in nulliparous women (17.0 versus 26.5 h, ES = 0.62 (0.10 to 1.14), p = 0.006). The rate of vaginal delivery <24 h significantly increased with combined induction (88.5% versus 66.6%, p = 0.03). No statistical difference was observed concerning caesarean rate (12.5% versus 17.5%, p > 0.05), chorioamnionitis (0% versus 2.5%, p = 1), postpartum endometritis, or maternal or neonatal outcomes. Procedure-related pain and tolerance to devices were found to be similar for the two methods. Interpretation: The double balloon catheter combined with oxytocin is an alternative for cervical ripening in case of PROM at term, and may reduce TID in nulliparous women.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/35329852; hal-03824651; https://hal.science/hal-03824651Test; https://hal.science/hal-03824651/documentTest; https://hal.science/hal-03824651/file/jcm-11-01525.pdfTest; PUBMED: 35329852; PUBMEDCENTRAL: PMC8952372

الإتاحة: https://doi.org/10.3390/jcm11061525Test
https://hal.science/hal-03824651Test
https://hal.science/hal-03824651/documentTest
https://hal.science/hal-03824651/file/jcm-11-01525.pdfTest

المؤلفون: Guiguet-Auclair, Candy, Rouzaire, Marion, Debost-Legrand, Anne, Dissard, Sigrid, Rouille, Manon, Delabaere, Amélie, Gallot, Denis

المساهمون: Institut Pascal (IP), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), CHU Clermont-Ferrand, Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), Service Obstétrique CHU Clermont-Ferrand, CHU Estaing Clermont-Ferrand, CHU Clermont-Ferrand-CHU Clermont-Ferrand

المصدر: ISSN: 2077-0383 ; Journal of Clinical Medicine ; https://uca.hal.science/hal-03747509Test ; Journal of Clinical Medicine, 2022, 11 (14), pp.4217. ⟨10.3390/jcm11144217⟩.

مصطلحات موضوعية: EXIT, Induction of labor, patient-reported outcome measure, cross-cultural adaptation, psychometric properties, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, [SDV.MHEP.GEO]Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics

الوصف: International audience ; Background: In France, more than 20% of women require induction of labor (IOL), which can be psychologically and emotionally challenging for patients. It is important to assess how they feel about their IOL experiences. Our aim was to cross-culturally adapt and evaluate the psychometric properties of a French version of the EXIT to assess women’s experiences of IOL. Methods: The EXIT was cross-culturally adapted by conducting forward and backward translations following international guidelines. A cross-sectional study was conducted to assess the psychometric properties of the ten French EXIT items: data completeness, factor analysis, internal consistency, score distribution, floor and ceiling effects, inter-subscale correlations, convergent validity, and test–retest reliability. Results: The EXIT was successfully cross-culturally adapted to the French context andany IOL method. The results obtained from 163 patients requiring IOL showed good acceptability. Exploratory factor analysis resulted in a three-factor solution with subscales reflecting the experiential aspects of time taken to give birth, discomfort with IOL, and subsequent contractions. Good internal consistency (Cronbach’s alpha or Spearman correlation coefficients ranging from 0.55 to 0.84) and good test–retest reliability (intraclass correlation coefficients ranging from 0.66 to 0.85) for the three identified subscales were found. Conclusions: The ten-item French EXIT is a valid and reliable instrument for the self-assessment of women’s experiences of IOL in the three weeks following delivery for any method of IOL used. As a patient-reported outcome measure, it would allow the comparison of experiential outcomes across IOL studies in order to include women’s preferences in decisions regarding their care.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/35887980; hal-03747509; https://uca.hal.science/hal-03747509Test; https://uca.hal.science/hal-03747509/documentTest; https://uca.hal.science/hal-03747509/file/jcm-11-04217-v3.pdfTest; PUBMED: 35887980; PUBMEDCENTRAL: PMC9317795

الإتاحة: https://doi.org/10.3390/jcm11144217Test
https://uca.hal.science/hal-03747509Test
https://uca.hal.science/hal-03747509/documentTest
https://uca.hal.science/hal-03747509/file/jcm-11-04217-v3.pdfTest

المؤلفون: Rouzaire, Marion, Corvaisier, Marion, Roumeau, Virginie, Mulliez, Aurélien, Sendy, Feras, Delabaere, Amélie, Gallot, Denis

المساهمون: Service de Gynécologie CHU Clermont-Ferrand, CHU Estaing Clermont-Ferrand, CHU Clermont-Ferrand-CHU Clermont-Ferrand, Service Obstétrique CHU Clermont-Ferrand, Centre Hospitalier Emile Roux Le Puy-en-Velay (CH Le Puy-en-Velay), Unité de Biostatistiques CHU Clermont-Ferrand, Direction de la recherche clinique et de l’innovation CHU Clermont-Ferrand (DRCI), Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)

المصدر: ISSN: 2077-0383 ; Journal of Clinical Medicine ; https://hal.science/hal-03824709Test ; Journal of Clinical Medicine, 2021, 10 (1), pp.150. ⟨10.3390/jcm10010150⟩.

مصطلحات موضوعية: latency, outpatient, predictive factors, preterm premature rupture of membranes, [SDV.MHEP.GEO]Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics

الوصف: International audience ; Background: Preterm premature rupture of membranes (PPROM) is a complication responsible for a third of preterm births. Clinical management is initially hospital based, but homecare management is possible if patients are clinically stable 48 h after PPROM. This study set out to determine factors that are predictive of short latency (delivery ≤ 7 days) exceeding 48 h after PPROM, enabling estimation of the prevalence of maternal and neonatal complications and comparison of maternal and fetal outcomes between inpatient and outpatient management. Method: This was a monocentric retrospective study conducted between 1 January 2010 and 28 February 2017 on all patients experiencing PPROM at 24 to 34 weeks + 6 days and who gave birth after 48 h. Maternal, obstetric, fetal, and neonatal variables were included in the data collected. The primary endpoint was latency, defined as the number of days between rupture of membranes and delivery. Results: 170 consecutive patients were analyzed. Short latency could be predicted by the need for tocolysis, a cervical length less than 25 mm at admission and the existence of anamnios. Outpatient follow-up was not found to lead to increased maternal morbidity or neonatal mortality. Conclusion: Our study highlights predictive factors of short latency exceeding 48 h after PPROM. Knowledge of these factors may provide justification for outpatient monitoring of patients presenting with a long cervix, absence of need for tocolysis and persistence of amniotic fluid and, thus, no risk factors after 48 h of admission.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/33406795; hal-03824709; https://hal.science/hal-03824709Test; https://hal.science/hal-03824709/documentTest; https://hal.science/hal-03824709/file/jcm-10-00150-v2.pdfTest; PUBMED: 33406795; PUBMEDCENTRAL: PMC7796089

الإتاحة: https://doi.org/10.3390/jcm10010150Test
https://hal.science/hal-03824709Test
https://hal.science/hal-03824709/documentTest
https://hal.science/hal-03824709/file/jcm-10-00150-v2.pdfTest

المؤلفون: Pambet, Mathilde, Sirodot, Fanny, Pereira, Bruno, Cahierc, Romain, Delabaere, Amélie, Comptour, Aurélie, Rouzaire, Marion, Sapin, Vincent, Gallot, Denis

المصدر: Journal of Clinical Medicine; Sep2023, Vol. 12 Issue 17, p5707, 11p

مصطلحات موضوعية: PREMATURE labor, CHILDBIRTH, UNIVERSITY hospitals, FIBRONECTINS, LONGITUDINAL method

مصطلحات جغرافية: CLERMONT-Ferrand (France)

مستخلص: We conducted a prospective double-blind study to compare two vaginal diagnostic methods in singleton pregnancies with threatened preterm labor (TPL) at the University Hospital of Clermont-Ferrand (France) from August 2018 to December 2020. Our main objective was to compare the diagnostic capacity at admission, in terms of positive predictive value (PPV) and negative predictive value (NPV), of Premaquick^® (combined detection of IL-6/total IGFBP-1/native IGFBP-1) and QuikCheck fFN™ (fetal fibronectin) for delivery within 7 days in cases of TPL. We included 193 patients. Premaquick^® had a sensitivity close to 89%, equivalent to QuikCheck fFN™, but a higher statistical specificity of 49.5% against 38.6% for QuikCheck fFN™. We found no superiority of Premaquick^® over QuickCheck fFN™ in terms of PPV (6.6% vs. 7.9%), with NPV being equivalent in predicting childbirth within 7 days in cases of TPL (98.6% vs. 98.9%). Nevertheless, the combination of positive native and total IGFBP-1 and the combination of all three positive markers were associated with a higher PPV. Our results, though non-significant, support this combined multiple-biomarker approach to improve testing in terms of predictive values. [ABSTRACT FROM AUTHOR]

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