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المؤلفون: Jeanine R. Jarnes, Caroline A. Hastings, Can H. Ficicioglu, Debra-Lynn Day-Salvatore, Roberto Giugliani, Julien Baruteau, Chester B. Whitley, Michal Inbar-Feigenberg, Geneviève Bernard, Fatih S. Ezgü, Yan G. Ni, Michelle Miller, Michael H. Gelb, Pruthvi Nagilla, Rose Johnstone, Patricia Elsasser, Elizabeth Cunningham, Victoria Ballard, Thomas F. Haws, Mark S. Forman, David A. Weinstein
المصدر: Molecular Genetics and Metabolism. 138:107169
مصطلحات موضوعية: Endocrinology, Endocrinology, Diabetes and Metabolism, Genetics, Molecular Biology, Biochemistry
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::2595e1d8f08ff57da247d7806faa3b67Test
https://doi.org/10.1016/j.ymgme.2022.107169Test -
2دورية أكاديمية
المؤلفون: Trairak Pisitkun, Rose Johnstone
المساهمون: The Pennsylvania State University CiteSeerX Archives
الوصف: A myriad of proteins and peptides can be identified in normal human urine. These are derived from a variety of sources including glomerular filtration of blood plasma, cell sloughing, apoptosis, proteolytic cleavage of cell sur-face glycosylphosphatidylinositol-linked proteins, and se-cretion of exosomes by epithelial cells. Mass spectrome-try-based approaches to urinary protein and peptide profiling can, in principle, reveal changes in excretion rates of specific proteins/peptides that can have predic-tive value in the clinical arena, e.g. in the early diagnosis of disease, in classification of disease with regard to likely therapeutic responses, in assessment of prognosis, and in monitoring response to therapy. These approaches have potential value, not only in diseases of the kidney and urinary tract but also in systemic diseases that are
وصف الملف: application/pdf
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المؤلفون: Rose, Johnstone
المصدر: Scientia canadensis. 27
مصطلحات موضوعية: Quebec, History, 19th Century, History, 20th Century, Biochemistry, Schools, Medical
الوصف: The Department of Biochemistry at McGill University was inaugurated close to a century after the Medical School was founded. The roots of the Department, however, can be found at the very beginning of the Medical School in 1829. Because several of the founding faculty members of the Medical School were educated in Edinburgh, McGill's early medical program bore the imprint of the Edinburgh school--particularly in the importance placed on instruction in chemistry and on basic research. This survey of the development of a university department is structured on the succession of department chairs, and describes how the Department's scientific, pedagogical, and administrative activities were influenced by the particular abilities and dispositions of the individuals who were at the helm. It explains how the growth of external research institutes influenced the Department's evolution, and cites some of the noteworthy contributions of its members.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::e779941aed59f2cd06a986883c89a4b0Test
https://pubmed.ncbi.nlm.nih.gov/16116702Test -
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المؤلفون: Fakhraddin, Naghibalhossaini, Francine, Nault, Uri, Saragovi, Haynu, Nedev, Rose, Johnstone
المصدر: Medical science monitor : international medical journal of experimental and clinical research. 8(11)
مصطلحات موضوعية: GABA Plasma Membrane Transport Proteins, DNA, Complementary, Saccharomyces cerevisiae Proteins, Time Factors, Molecular Sequence Data, Organic Anion Transporters, Saccharomyces cerevisiae, Fungal Proteins, Ethidium, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular, Codon, Electrophoresis, Agar Gel, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Membrane Proteins, Membrane Transport Proteins, Blotting, Northern, Intercalating Agents, Protein Structure, Tertiary, Mutation, DNA Transposable Elements, RNA, Carrier Proteins, Peptides
الوصف: The Saccharomyces cerevisiae strain, 22574d, lacks gamma amino butyric acid (GABA) transport activity and cannot grow on this amino acid as sole nitrogen source. Both transport of and growth on this amino acid are restored when the yeast is transformed with a form of mouse gamma actin containing an extended C-terminal sequence (M-g-A). The nature of the mutation in the transporter as well as the complementation mode are addressed.The cDNA sequence of the mutated transporter was achieved using reverse transcription, 3'-Race and cloning. For detection, Northern blotting and labeling with 32-P were used. The putative ability of the transporter to interact with gamma actin in vivo was examined by following the interaction in vitro of synthetic peptides corresponding to the C-termini of the gamma actin and the transporter.Up to codon 394 the mutated and native transporters are identical. At the 3' end, the mutant is by extended by 32 codons from the delta region of a Ty1 transposon, giving an open reading frame of 426 codons, and a predicted structure of 9 of the 11 transmembrane domains. Peptides corresponding to the carboxy terminal regions of the truncated transporter and the elongated species of gamma actin show the potential to interact in vitro.The mutated GABA transporter mRNA contains an insert from the delta region of a Ty1 transposon. This insertion allows expression of a transporter capable of interacting with elongated gamma actin to rehabilitate the transporter.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::2335bb916bf85b949f768087419e16ecTest
https://pubmed.ncbi.nlm.nih.gov/12444371Test
