المؤلفون: Andrea De Luca, Alessandro Ferraro, Chiara De Gregorio, Mariateresa Laborante, Marco Coassin, Roberto Sgrulletta, Antonio Di Zazzo

المصدر: Life, Vol 13, Iss 7, p 1425 (2023)

مصطلحات موضوعية: dry eye disease, diagnostic device, ocular surface, Science

الوصف: Background: Dry eye disease (DED) is a common and debilitating condition that affects millions of people worldwide. Despite its prevalence, the diagnosis and management of DED can be challenging, as the condition is multifactorial and symptoms can be nonspecific. In recent years, there have been significant advancements in diagnostic technology for DED, including the development of several new devices. Methods: A literature review of articles on the dry eye syndrome and innovative diagnostic devices was carried out to provide an overview of some of the current high-tech diagnostic tools for DED, specifically focusing on the TearLab Osmolarity System, DEvice Hygrometer, IDRA, Tearcheck, Keratograph 5M, Cornea Dome Lens Imaging System, I-PEN Osmolarity System, LipiView II interferometer, LacryDiag Ocular Surface Analyzer, Tearscope-Plus, and Cobra HD Camera. Conclusions: Despite the fact that consistent use of these tools in clinical settings could facilitate diagnosis, no diagnostic device can replace the TFOS algorithm.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2075-1729/13/7/1425Test; https://doaj.org/toc/2075-1729Test

الوصول الحر: https://doaj.org/article/a6aba74f3314471aa2fcce0844328eeaTest

المؤلفون: Daniele Gaudenzi, Tommaso Mori, Salvatore Crugliano, Antonella Grasso, Carlo Frontini, Antonella Carducci, Siddharth Yadav, Roberto Sgrulletta, Emiliano Schena, Marco Coassin, Antonio Di Zazzo

المصدر: Applied Sciences, Vol 12, Iss 3, p 1647 (2022)

مصطلحات موضوعية: dry eye disease, AS-OCT, ocular hygrometer, Technology, Engineering (General). Civil engineering (General), TA1-2040, Biology (General), QH301-705.5, Physics, QC1-999, Chemistry, QD1-999

الوصف: Dry eye disease (DED) is one of the conditions that most commonly leads patients to visit an ophthalmologist. Fast and accurate diagnosis relieves patient discomfort and spares them from long-term effects on the ocular surface. Many tests used in the diagnosis of DED may be considered subjective as they rely on an experienced observer for image interpretation, resulting in variations in diagnosis. On one hand, the non-contact nature of the anterior segment optical coherence tomography (AS-OCT) device and its rapid image acquisition enable the measurement of the tear meniscus parameter without reflex tearing. On the other hand, an ocular hygrometer allows a rapid, safe, but also efficient, analysis and is associated with low costs and the repeatability of the procedure.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2076-3417/12/3/1647Test; https://doaj.org/toc/2076-3417Test

الوصول الحر: https://doaj.org/article/d3b2c4b21717465282927ce2b0c03c04Test

المؤلفون: Marco Antonini, Daniele Gaudenzi, Sara Spelta, Giancarlo Sborgia, Maria Poddi, Alessandra Micera, Roberto Sgrulletta, Marco Coassin, Antonio Di Zazzo

المصدر: Journal of Clinical Medicine, Vol 10, Iss 14, p 3048 (2021)

مصطلحات موضوعية: para-inflammation, ocular surface, glycerophosphoinositol, urban syndrome, Medicine

الوصف: Background: Nowadays, the continuous increase in air pollution has significantly changed air quality, leading to the onset of the so-called urban syndrome (US), an allergic-like conjunctivitis triggered by pollutants. These patients are characterized by persistent dysregulation of ocular surface para-inflammation, causing chronic low-grade inflammation and ocular discomfort, with significant consequences for occupational health and job productivity prospects. This study aims to investigate the effects of topical glycerophosphoinositol (GPI) eye drops on the signs and symptoms of US. Methods: A multicenter prospective open interventional study was performed. Patients affected by US, enrolled from occupational medicine clinics, were treated with eye drops containing 0.001% GPI in 0.2% HA vehicle three times a day. Ocular surface disease index (OSDI), tear break-up time (T-BUT), Schirmer test, Oxford score, hyperemia and ocular surface symptoms were recorded at patient enrolment (T0), after 1 week (T1) and after 1 month (T2) of treatment. Results: A total of 113 consecutive patients (226 eyes) were included. OSDI score displayed a significant improvement after one week (T0: 39.9 ± 19, T1: 20.8 ± 17.9, T2: 18.4 ± 15.6, p < 0.0001); T-BUT (T0: 5.2 ± 2, T1: 7.7 ± 2.2, T2: 9.7 ± 1.8, p < 0.0001) and Schirmer Test (T0: 6.6 ± 2.4, T1: 9.7 ± 2.7, T2: 12.6 ± 2.6, p < 0.0001) progressively improved from T0 to T2. Conclusions: trice-daily topic instillation of 0.001% GPI in 0.02% HA vehicle resulted an effective and well tolerated treatment in US patients.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2077-0383/10/14/3048Test; https://doaj.org/toc/2077-0383Test

الوصول الحر: https://doaj.org/article/b9d1e20ddca14047ae1218bec2ed727fTest

المؤلفون: Alessandra Micera, Barbara Stampachiacchiere, Antonio Di Zazzo, Roberto Sgrulletta, Magdalena Cortes, Eduardo Maria Normando, Alessandro Lambiase, Stefano Bonini

المصدر: PLoS ONE, Vol 10, Iss 11, p e0142737 (2015)

مصطلحات موضوعية: Medicine, Science

الوصف: In a previous study, we reported the upregulation of Nerve Growth Factor (NGF) and trkANGFR expression in Ocular Cicatricial Pemphigoid (OCP), an inflammatory and remodeling eye disease. Herein, we hypothesize a potential NGF-driven mechanism on fibroblasts (FBs) during OCP remodeling events. To verify, human derived OCP-FBs were isolated and characterized either at baseline or after NGF exposure.Conjunctival biopsies were obtained from 7 patients having OCP and 6 control subjects (cataract surgery). Both conjunctivas and primary FB cultures were characterised for αSMA, NGF and trkANGFR/p75NTR expression. Subcultures were exposed to NGF and evaluated for αSMA, NGF, trkANGFR/p75NTR expression as well as TGFβ1/IL4 release. For analysis, early and advanced subgroups were defined according to clinical parameters.OCP-conjunctivas showed αSMA-expressing FBs and high NGF levels. Advanced OCP-FBs showed higher αSMA expression associated with higher p75NTR and lower trkANGFR expression, as compared to early counterparts. αSMA expression was in keeping with disease severity and correlated to p75NTR. NGF exposure did not affect trkANGFR levels in early OCP-FBs while decreased both αSMA/p75NTR expression and TGFβ1/IL4 release. These effects were not observed in advanced OCP-FBs.Taken together, these data are suggestive for a NGF/p75NTR task in the potential modulation of OCP fibrosis and encourages further studies to fully understand the underlying mechanism occurring in fibrosis. NGF/p75NTR might be viewed as a potential therapeutic target.

وصف الملف: electronic resource

العلاقة: http://europepmc.org/articles/PMC4646573?pdf=renderTest; https://doaj.org/toc/1932-6203Test

الوصول الحر: https://doaj.org/article/cdd4553ae6ff496db2d3c66c5e04a585Test

المؤلفون: Tommaso, Mori, Francesco, Cutrupi, Pier Luigi, Surico, Roberto, Sgrulletta, Antonio, Di Zazzo, Marco, Coassin

المصدر: RETINAL Cases & Brief Reports.

مصطلحات موضوعية: Ophthalmology, General Medicine

الوصف: To report three cases of massive pseudophakic macular edema occurring the day after uneventful cataract surgery and resolving in 24-72 hours.A 68-years woman affected by systemic lupus erythematosus and antiphospholipid syndrome displayed massive macular edema on OCT scan one day after uneventful cataract surgery. Routine postoperative topical eye drops (Chloramphenicol/Betamethasone QID and Bromfenac BID) were continued without additional medications. Three days later OCT showed a completely recovered, normal fovea. Two similar cases were documented. A 73-year-old man and a 53-year-old man underwent cataract surgery and started the mentioned topical postoperative therapy. Severe macular edema was diagnosed the day after surgery and resolved in 24 and 48 hours, respectively.Massive macular edema may occur immediately after uncomplicated cataract and then disappear within 1 to 3 days, without invasive therapies. This is a very significant event that may follow cataract surgery that was previously unreported.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a06248bd84474a4376aea0bb08164ec5Test
https://doi.org/10.1097/icb.0000000000001324Test

المؤلفون: Alessandra Micera, Antonio Di Zazzo, Graziana Esposito, Roberto Sgrulletta, Virginia L. Calder, Stefano Bonini

المصدر: BioMed Research International, Vol 2016 (2016)

مصطلحات موضوعية: Medicine

الوصف: Objective. Vernal keratoconjunctivitis (VKC) is a chronic recurrent bilateral inflammation of the conjunctiva associated with atopy. Several inflammatory and tissue remodeling factors contribute to VKC disease. The aim is to provide a chip-based protein analysis in tears from patients suffering from quiescent or active VKC. Methods. This study cohort included 16 consecutive patients with VKC and 10 controls. Participants were subjected to clinical assessment of ocular surface and tear sampling. Total protein quantification, total protein sketch, and protein array (sixty protein candidates) were evaluated. Results. An overall increased Fluorescent Intensity expression was observed in VKC arrays. Particularly, IL1β, IL15, IL21, Eotaxin2, TACE, MIP1α, MIP3α, NCAM1, ICAM2, βNGF, NT4, BDNF, βFGF, SCF, MMP1, and MMP2 were increased in quiescent VKC. Of those candidates, only IL1β, IL15, IL21, βNGF, SCF, MMP2, Eotaxin2, TACE, MIP1α, MIP3α, NCAM1, and ICAM2 were increased in both active and quiescent VKC. Finally, NT4, βFGF, and MMP1 were highly increased in active VKC. Conclusion. A distinct “protein tear-print” characterizes VKC activity, confirming some previously reported factors and highlighting some new candidates common to quiescent and active states. Those candidates expressed in quiescent VKC might be considered as predictive indicators of VKC reactivation and/or exacerbation out-of-season.

العلاقة: http://dx.doi.org/10.1155/2016/9672082Test; https://doaj.org/toc/2314-6133Test; https://doaj.org/toc/2314-6141Test; https://doaj.org/article/f3d87b18533348bd825ed3d3131cc1f6Test

الإتاحة: https://doi.org/10.1155/2016/9672082Test
https://doaj.org/article/f3d87b18533348bd825ed3d3131cc1f6Test

المؤلفون: Flavio Mantelli, Myrna S Santos, Tommasangelo Petitti, Roberto Sgrulletta, Magdalena Cortes, Ro Lambiase, Sergio Bonini, Stefano Bonini, Email Alerting

المساهمون: The Pennsylvania State University CiteSeerX Archives

المصدر: http://www.v2020la.org/pub/PUBLICATIONS_BY_TOPICS/VARIOUS/SystematicTest review.pdf.

مصطلحات موضوعية: Meta-analysis, VKC, vern

الوصف: Systematic review and meta-analysis of randomized clinical trials on topical treatments for vernal keratoconjunctivitis

وصف الملف: application/pdf

العلاقة: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.376.101Test; http://www.v2020la.org/pub/PUBLICATIONS_BY_TOPICS/VARIOUS/SystematicTest review.pdf

الإتاحة: http://www.v2020la.org/pub/PUBLICATIONS_BY_TOPICS/VARIOUS/SystematicTest review.pdf

المؤلفون: Ra Micera, Ro Lambiase, Barbara Stampachiacchiere, Roberto Sgrulletta, Eduardo Maria, Sergio Bonini, Stefano Bonini, Irccs-g. B. Bietti, Eye Foundation

المساهمون: The Pennsylvania State University CiteSeerX Archives

المصدر: ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/1a/59/Mol_Vis_2007_Jun_21_13_981-987.tar.gz

الوصف: Nerve growth factor has a modulatory role on human primary fibroblast cultures derived from vernal keratoconjunctivitisaffected conjunctiva

وصف الملف: application/zip

العلاقة: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.356.8422Test

المؤلفون: Marta Sacchetti, Alessandro Lambiase, Magdalena Cortes, Roberto Sgrulletta, Sergio Bonini, Daniela Merlo, Stefano Bonini

المصدر: Graefe's Archive of Clinical & Experimental Ophthalmology; Sep2005, Vol. 243 Issue 9, p870-876, 7p