المؤلفون: Lateef, Adebola Azeez, Azeez, Abiodun Abeeb, Ren, Wenzi, Hamisu, Habu, Oke, Abiola, Asiegbu, Fred

المساهمون: Department of Forest Sciences, Doctoral Programme in Plant Sciences, Helsinki Institute of Sustainability Science (HELSUS), Viikki Plant Science Centre (ViPS), Frederick Asiegbu / Principal Investigator, Forest Ecology and Management

مصطلحات موضوعية: 11831 Plant biology, 415 Other agricultural sciences

الوصف: Peer reviewed

وصف الملف: application/pdf

العلاقة: Lateef , A A , Azeez , A A , Ren , W , Hamisu , H , Oke , A & Asiegbu , F 2024 , ' Bacterial biota associated with the invasive insect pest Tuta absoluta (Meyrick) ' , Scientific Reports , vol. 14 , 8268 , pp. 1-14 . https://doi.org/10.1038/s41598-024-58753-wTest; ORCID: /0000-0002-0510-7996/work/158611579; ORCID: /0000-0003-0223-7194/work/158618390; http://hdl.handle.net/10138/574945Test; 8a25301f-8ef6-4a66-b5bc-1098e4fdd98b

الإتاحة: http://hdl.handle.net/10138/574945Test

المؤلفون: Ugwu, Juliana Amaka, Ren, Wenzi, Asiegbu, Fred

المساهمون: Department of Forest Sciences, Helsinki Institute of Sustainability Science (HELSUS), Viikki Plant Science Centre (ViPS), Frederick Asiegbu / Principal Investigator, Forest Ecology and Management

مصطلحات موضوعية: LIFE, MICROBIOTA, MIDGUT, MOTH, PHYSIOLOGY, PLANT, POPULATIONS, TAXONOMY, TRADE-OFFS, WILD, bacteria, fall armyworm, lab-reared larvae, monocot, plant diets, 1181 Ecology, evolutionary biology

الوصف: Microbiome research is currently gaining tremendous interest on the impact of diets and environment to influence life traits of several hosts. Full understanding of the insect ecology and characterization of gut microbial community including their natural enemies is vital for development of novel insect pest management strategies. This study examined the gut bacterial communities of Fall armyworm (FAW) Spodoptera frugiperda reared in the laboratory with three monocot plant diets (Sugarcane [M1], maize [M2] and onion [M3]) using 16S rRNA gene sequencing. The study revealed variations in the structure and community of the gut bacterial biota of FAW larvae reared with three monocot plants. Diet sources greatly altered the structure of the gut bacterial community. Three alpha diversity indices (observed species richness Chao1 and ACE) showed significant differences among the various diet groups. The principal component analysis revealed a moderate distinction in bacterial communities of the FAW larvae reared with the three monocot plants. Higher numbers of operational taxonomic units (OTUs) were observed in sugarcane diet (M1). The bacterial communities of all the larvae groups were dominated by phylum Firmicutes. A redundancy analysis (RDA) indicated that neutral detergent fibre (NDF) (p < 0.0005) was the most significant factors influencing the community distribution of gut bacteria of FAW larvae. Functional Annotation of Prokaryotic Taxa (FAPROTAX) analysis predicted that chemoheterotrophy, fermentation, aerobic chemoheterotrophy and aromatic compound degradation were the prominent putative functions of FAW larvae gut bacteria communities. Chemoheterotrophy was the most dominant putative functions predicted and was higher in sugarcane diet. Furthermore, the complexity of the network structure was higher in M1 while the modularity was higher in maize diet. The different monocot diets had a considerable impact on bacterial composition in the gut of Fall armyworm larvae and their ecological and functional ...

وصف الملف: application/pdf

العلاقة: Ugwu , J A , Ren , W & Asiegbu , F 2022 , ' Monocot diet sources drive diversity of gut bacterial communities in Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae ' , Journal of Applied Entomology , vol. 146 , no. 8 , pp. 942-956 . https://doi.org/10.1111/jen.13022Test; ORCID: /0000-0003-0223-7194/work/117943406; b39a8518-de82-4084-ada4-0be1fbdd4341; http://hdl.handle.net/10138/357929Test; 000799492200001

الإتاحة: http://hdl.handle.net/10138/357929Test

المؤلفون: Ren, Wenzi, Penttilä, Reijo, Kasanen, Risto, Asiegbu, Fred

المساهمون: Department of Forest Sciences, Helsinki Institute of Sustainability Science (HELSUS), Viikki Plant Science Centre (ViPS), Frederick Asiegbu / Principal Investigator, Forest Ecology and Management, Doctoral Programme in Integrative Life Science, Doctoral Programme in Plant Sciences, Doctoral Programme in Sustainable Use of Renewable Natural Resources

مصطلحات موضوعية: 11832 Microbiology and virology

الوصف: Peer reviewed

وصف الملف: application/pdf

العلاقة: Ren , W , Penttilä , R , Kasanen , R & Asiegbu , F 2023 , ' Interkingdom and intrakingdom interactions in the microbiome of Heterobasidion fruiting body and associated decayed woody tissues ' , Applied and Environmental Microbiology . https://doi.org/10.1128/aem.01406-23Test; ORCID: /0000-0003-0223-7194/work/148362716; ORCID: /0000-0002-9948-2200/work/148363373; 902e0fe6-cd11-4e7e-8368-884ef730bc42; http://hdl.handle.net/10138/568015Test

الإتاحة: http://hdl.handle.net/10138/568015Test

المؤلفون: Ren, Wenzi, Penttilä, Reijo, Kasanen, Risto, Asiegbu, Fred

المساهمون: Department of Forest Sciences, Forest Ecology and Management, Helsinki Institute of Sustainability Science (HELSUS), Viikki Plant Science Centre (ViPS), Frederick Asiegbu / Principal Investigator

مصطلحات موضوعية: 4112 Forestry, 11832 Microbiology and virology, wood decay process, bacteria community, Heterobasidion, fruiting body, network analysis, NORWAY SPRUCE, PINUS-SYLVESTRIS, FUNGAL PATHOGEN, SCOTS PINE, SOIL, ROOT, PARVIPORUM, RESISTANCE, INFECTION, ANNOSUM

الوصف: The microbiome of Heterobasidion-induced wood decay of living trees has been previously studied; however, less is known about the bacteria biota of its perennial fruiting body and the adhering wood tissue. In this study, we investigated the bacteria biota of the Heterobasidion fruiting body and its adhering deadwood. Out of 7,462 operational taxonomic units (OTUs), about 5,918 OTUs were obtained from the fruiting body and 5,469 OTUs were obtained from the associated dead wood. Interestingly, an average of 52.6% of bacteria biota in the fruiting body was shared with the associated dead wood. The overall and unique OTUs had trends of decreasing from decay classes 1 to 3 but increasing in decay class 4. The fruiting body had the highest overall and unique OTUs number in the fourth decay class, whereas wood had the highest OTU in decay class 1. Sphingomonas spp. was significantly higher in the fruiting body, and phylum Firmicutes was more dominant in wood tissue. The FAPROTAX functional structure analysis revealed nutrition, energy, degradation, and plant-pathogen-related functions of the communities. Our results also showed that bacteria communities in both substrates experienced a process of a new community reconstruction through the various decay stages. The process was not synchronic in the two substrates, but the community structures and functions were well-differentiated in the final decay class. The bacteria community was highly dynamic; the microbiota activeness, community stability, and functions changed with the decay process. The third decay class was an important turning point for community restructuring. Host properties, environmental factors, and microbial interactions jointly influenced the final community structure. Bacteria community in the fruiting body attached to the living standing tree was suppressed compared with those associated with dead wood. Bacteria appear to spread from wood tissue of the standing living tree to the fruiting body, but after the tree is killed, bacteria moved from ...

وصف الملف: application/pdf

العلاقة: Ren , W , Penttilä , R , Kasanen , R & Asiegbu , F 2022 , ' Bacteria Community Inhabiting Heterobasidion Fruiting Body and Associated Wood of Different Decay Classes ' , Frontiers in Microbiology , vol. 13 , 864619 . https://doi.org/10.3389/fmicb.2022.864619Test; ORCID: /0000-0003-0223-7194/work/114298852; ORCID: /0000-0002-9948-2200/work/114299722; 25afa7f0-2cf0-4328-9772-27c3f1b5df1b; http://hdl.handle.net/10138/344396Test; 000796969900001

الإتاحة: http://hdl.handle.net/10138/344396Test

المؤلفون: Azeez, Abiodun Abeeb, Esiegbuya, Daniel Ofeoritse, Jaber, Emad, Ren, Wenzi, Lateef, Adebola Azeez, Ojieabu, Amarachi, Asiegbu, Fred O.

الوصف: Several economically important diseases of forest trees and agricultural crops in many parts of the world have been linked to the ascomycete fungal pathogen Thielaviopsis paradoxa. This study compared the growth rate of 41 isolates of T. paradoxa sourced from different hosts and two countries (Nigeria and Papua New Guinea (PNG)) under six temperature levels (22 °C, 25 °C, 30 °C, 32 °C, 34 °C and 35 °C). Phylogenetic relationships were obtained from the analysis of their nuclear ribosomal DNA internal transcribed sequence (ITS) data. While all the isolates from PNG and few from Nigeria grew optimally between 22 °C and 32 °C, the majority had their highest growth rate (2.9 cm/day) between 25 °C and 32 °C. Growth performances were generally low between 34 °C and 35 °C; no isolate from the sugar cane grew at these high temperatures. The oil palm isolate DA029 was the most resilient, with the highest growth rate (0.97 cm/day) at 35 °C. Phylogenetic analysis delineated five clusters: a very large clade which accommodates the majority (30 Nigerian and 3 PNG oil palm isolates) and four small clades containing two members each. To a large extent, the clustering pattern failed to address the temperature–isolate relationship observed. However, only the four small clades represent isolates with similar temperature tolerances. It is most likely that wider and robust analyses with more diverse isolates and genetic markers will provide better insight on thermal resilience of T. paradoxa. Additionally, future research to establish relationships between vegetative growth at different temperatures and of different pathogenicity and disease epidemiology merits being explored. The results might provide useful information for the formulation of effective management and control strategies against the pathogen, especially in this era of climate change.

وصف الملف: application/pdf

العلاقة: Azeez, A.A.; Esiegbuya, D.O.; Jaber, E.; Ren, W.; Lateef, A.A.; Ojieabu, A.; Asiegbu, F.O. Thermal Tolerance Data and Molecular Identification Are Useful for the Diagnosis, Control and Modeling of Diseases Caused by Thielaviopsis paradoxa. Pathogens 2023, 12, 727.; http://hdl.handle.net/10138/358125Test

الإتاحة: http://hdl.handle.net/10138/358125Test

المؤلفون: Hou, Yike, Tang, Zhe, Farheen, Jabeen, Saeed, Madiha, Luo, Lijia, Ren, Wenzi, Luo, Dandan, Mushtaq, Asim, Zhao, Ruibo, Ge, Jian, Yu, Zhangsen, Li, Yao, Iqbal, M. Zubair, Kong, Xiangdong

المصدر: Journal of Materials Science & Technology ; volume 148, page 123-137 ; ISSN 1005-0302

مصطلحات موضوعية: Materials Chemistry, Metals and Alloys, Polymers and Plastics, Mechanical Engineering, Mechanics of Materials, Ceramics and Composites

الإتاحة: https://doi.org/10.1016/j.jmst.2022.11.024Test
https://api.elsevier.com/content/article/PII:S1005030223000300?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S1005030223000300?httpAccept=text/plainTest

المؤلفون: Ren, Wenzi

المساهمون: Casero, Julio Javier Diez, Asiegbu, Fred, Wang, Kai, Helsingin yliopisto, maatalous-metsätieteellinen tiedekunta, Uusiutuvien luonnonvarojen kestävän käytön tohtoriohjelma, Helsingfors universitet, agrikultur-forstvetenskapliga fakulteten, Doktorandprogrammet i hållbart utnyttjande av förnybara naturresurser, University of Helsinki, Faculty of Agriculture and Forestry, Doctoral Programme in Sustainable Use of Renewable Natural Resources

مصطلحات موضوعية: molecular Forest Pathology

الوصف: Coniferous forests are crucial for the planet, with their worldwide distribution and enormous economic and ecological values. Despite their global spread and adaptability, conifers have faced several challenges due to biotic and abiotic environmental stressors. One of the major biotic pathogens is Heterobasidion annosum s. l., the causative agent of root and stem rot disease. The economic damage caused by Heterobasidion is estimated at €800 million in Europe annually. Over the years, the disease has been managed using silviculture, chemical, and biological approaches. Unfortunately, there is no 100% protection, and all the methods have defects or potential risks. Thus, new solutions need to be explored. Understanding and manipulation of the microbiome inhabiting the conifer host and the Heterobasidion pathosystem could provide new insight for troubleshooting and is considered to have huge potential. The aims of this study were to investigate the bacterial and fungal community assembly and structure in the Heterobasidion-conifer pathosystem and to highlight the key factors driving microbial community development. Two sets of materials were studied: the Heterobasidion fruiting body and its associated wood of four different decay classes as well as the associated forest soil. Heterobasidion-infected wood samples were collected from managed and unmanaged forest sites in southern Finland. In addition, microbiome of Norway spruce seedling needles with different levels of resistance to Heterobasidion infection were also studied. The results revealed that the bacterial and fungal communities inhabiting Heterobasidion fruiting bodies and associated decayed wood and soil materials were not influenced by the decay process. However, the communities were highly dynamic, and the abundance, functions, and ecological correlation networks of the operational taxonomic units (OTUs: clusters of sequencing reads that differ by less than 3% dissimilarity threshold) frequently changed during the decay process. The third decay class ...

وصف الملف: application/pdf

العلاقة: URN:ISBN:978-951-51-9696-5; 2024; Helsinki: Helsingin yliopisto, 2024, Dissertationes Universitatis Helsingiensis. 2954-2898; Dissertationes Universitatis Helsingiensis; URN:ISSN:2954-2952; URN:ISBN:978-951-51-9695-8; http://hdl.handle.net/10138/572515Test

الإتاحة: http://hdl.handle.net/10138/572515Test

المؤلفون: Ren, Wenzi

مصطلحات موضوعية: Armillaria, species identification, species distribution, DNA techniques, pairing tests

الوصف: Armillaria is a genus from the phylum Basidiomycota, which can cause Armillaria root rot disease. Both broadleaves and conifers are hosts for Armillaria. Some Armillaria species are important root and butt rot pathogens, causing mortality and yield reduction in forests. Others have more of a role as a saprophyte, helping to degrade woody substrate and therefore have some ecological importance. Knowing which species of Armillaria is present is important to determine any potential impact in forest regeneration. The aim of this thesis is to study the diversity and distribution of Armillaria species in the forest reserves at Dalby Söderskog and Norreskog in southern Sweden Two different methods were used in this study, one is based on the molecular techniques where the ITS region and EF-1ɑ region of samples’ DNA were amplified for sequencing. The amplified DNA sequences were queried in the Gene bank. The other method is based on the biological species concept which is realized by pairing tests. According to the results, Armillaria gallica, A. cepistipes and additional Armillaria sp. were identified in the study area and A. gallica is dominating. Armillaria gallica is distributed both in Söderskog and Norreskog, while A. cepistipes was only found in Söderskog. The morphology of rhizomorphs of Armillaria in cultures varied among isolate. Two genets were identified using traditional pairing tests and the genet sizes could be large enough to cover the whole study site. The Armillaria species identified in this thesis have an important role as saprophytes, decomposing dead organic matter, but can also behave as facultative parasites if trees are weakened by other stress factors. Several tree species are currently suffering from other biotic stressors (e.g. Hymenoscyphus fraxineus on ash, Dutch elm disease on elm, Phytophthora spp. on beech), which may make tees more susceptible to attack by Armillaria species in the future. However, given the typical lower virulence associated with the identified species at Dalby, any ...

العلاقة: https://stud.epsilon.slu.se/15532Test/

الإتاحة: https://stud.epsilon.slu.se/15532Test/

المؤلفون: Ren, Wenzi, Penttilä, Reijo, Kasanen, Risto, Asiegbu, Fred O.

المساهمون: 4100110710, Luonnonvarakeskus

مصطلحات موضوعية: Heterobasidion, interkingdom, intrakingdom, microbiome interaction, wood decay process

وصف الملف: false

العلاقة: Applied and Environmental Microbiology; 12; 89; e01406-23; https://jukuri.luke.fi/handle/10024/554466Test

الإتاحة: https://jukuri.luke.fi/handle/10024/554466Test