المؤلفون: Vijay Kondreddy, Rajkumar Banerjee, B. L. A. Prabhavathi Devi, Kathirvel Muralidharan, Selvakumar Piramanayagam

المصدر: Cell Communication and Signaling, Vol 22, Iss 1, Pp 1-18 (2024)

مصطلحات موضوعية: LPCAT3, Lipid nanoparticles, MALT1, Cytokines, Eicosanoids, Osteoarthritis, Medicine, Cytology, QH573-671

الوصف: Abstract The proinflammatory cytokines and arachidonic acid (AA)-derived eicosanoids play a key role in cartilage degeneration in osteoarthritis (OA). The lysophosphatidylcholine acyltransferase 3 (LPCAT3) preferentially incorporates AA into the membranes. Our recent studies showed that MALT1 [mucosa-associated lymphoid tissue lymphoma translocation protein 1]) plays a crucial role in propagating inflammatory signaling triggered by IL-1β and other inflammatory mediators in endothelial cells. The present study shows that LPCAT3 expression was up-regulated in both human and mice articular cartilage of OA, and correlated with severity of OA. The IL-1β-induces cell death via upregulation of LPCAT3, MMP3, ADAMTS5, and eicosanoids via MALT1. Gene silencing or pharmacological inhibition of LPCAT3 or MALT1 in chondrocytes and human cartilage explants notably suppressed the IL-1β-induced cartilage catabolism through inhibition of expression of MMP3, ADAMTS5, and also secretion of cytokines and eicosanoids. Mechanistically, overexpression of MALT1 in chondrocytes significantly upregulated the expression of LPCAT3 along with MMP3 and ADAMTS5 via c-Myc. Inhibition of c-Myc suppressed the IL-1β-MALT1-dependent upregulation of LPCAT3, MMP3 and ADAMTS5. Consistent with the in vitro data, pharmacological inhibition of MALT1 or gene silencing of LPCAT3 using siRNA-lipid nanoparticles suppressed the synovial articular cartilage erosion, pro-inflammatory cytokines, and eicosanoids such as PGE2, LTB4, and attenuated osteoarthritis induced by the destabilization of the medial meniscus in mice. Overall, our data reveal a previously unrecognized role of the MALT1-LPCAT3 axis in osteoarthritis. Targeting the MALT1-LPCAT3 pathway with MALT1 inhibitors or siRNA-liposomes of LPCAT3 may become an effective strategy to treat OA by suppressing eicosanoids, matrix-degrading enzymes, and proinflammatory cytokines. Graphical Abstract

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1478-811XTest

الوصول الحر: https://doaj.org/article/52472aa7c2d5446e9b54ee1db13f2dc1Test

المؤلفون: Arpita Poddar, Rajkumar Banerjee, Ravi Shukla

المصدر: Frontiers in Bioengineering and Biotechnology, Vol 11 (2023)

مصطلحات موضوعية: gene therapy, non-viral vector, nanomaterial, nucleic acid (DNA and RNA), delivery system, nanoparticle, Biotechnology, TP248.13-248.65

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fbioe.2023.1304769/fullTest; https://doaj.org/toc/2296-4185Test

الوصول الحر: https://doaj.org/article/7f4b6a2a65874771844c208172a28bccTest

المؤلفون: Ajaz Ahmad, Basit Latief Jan, Mohammad Raish, Hari Krishna Reddy Rachamalla, Rajkumar Banerjee, Debabrata Mukhopadhyay, Khalid M. Alkharfy

المصدر: Saudi Pharmaceutical Journal, Vol 27, Iss 5, Pp 637-642 (2019)

مصطلحات موضوعية: Therapeutics. Pharmacology, RM1-950

الوصف: The genotoxic potential of glucocorticoid receptor (GR)-targeted liposomal formulations of the anticancer drug molecule ESC8 was studied in vivo. A methodical literature review discovered no previous studies on the genotoxicity of ESC8. Genotoxicity was assessed in both male and female mice by various assay systems, such as comet assay, chromosomal aberrations and micronuclei assay, which detect different abnormalities. Eleven groups of male mice and eleven groups of female mice, containing six animals per group, were used in the present study: group I served as vehicle control; group II received the positive control (cyclophosphamide 40 mg/kg; CYP); and animals in group III to XI received free drug (ESC8), DX liposome and drug-associated DX liposomal formulation (DXE), respectively, dissolved in 5% solution of glucose at a drug-dose of 1.83, 3.67 and 7.34 mg/kg, respectively. Same drug treatments were followed for the female mice groups. The obtained data revealed the safety of DXE, which did not show substantial genotoxic effects at different dose levels. In contrast, the positive control, CYP, exhibited highly substantial irregular cytogenetic variations in comparison with the control group in different assays. Keywords: Liposomal formulation (DXE), Comet assay, Micronuclei, Chromosomal aberrations, Cyclophosphamide

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S131901641930043XTest; https://doaj.org/toc/1319-0164Test

الوصول الحر: https://doaj.org/article/9e0388c4129a4e57986ed0706368e32fTest

المؤلفون: Priya Dharmalingam, Hari Krishna R. Rachamalla, Brijesh Lohchania, Bhanuprasad Bandlamudi, Saravanabhavan Thangavel, Mohankumar K. Murugesan, Rajkumar Banerjee, Arabinda Chaudhuri, Chandrashekhar Voshavar, Srujan Marepally

المصدر: ACS Omega, Vol 2, Iss 11, Pp 7892-7903 (2017)

مصطلحات موضوعية: Chemistry, QD1-999

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2470-1343Test

الوصول الحر: https://doaj.org/article/dca52085134a46539b2b0f0d9c75612cTest

المؤلفون: Santhosh Chandar Maddila, Chandrashekhar Voshavar, Porkizhi Arjunan, Rashmi Prakash Chowath, Hari Krishna Reddy Rachamalla, Balaji Balakrishnan, Poonkuzhali Balasubramanian, Rajkumar Banerjee, Srujan Marepally

المصدر: Molecules, Vol 26, Iss 15, p 4626 (2021)

مصطلحات موضوعية: cationic lipids, clathrin-mediated endocytosis, caveolae-mediated endocytosis, macropinocytosis, transfection, endothelial cells, Organic chemistry, QD241-441

الوصف: Delivering nucleic acids into the endothelium has great potential in treating vascular diseases. However, endothelial cells, which line the vasculature, are considered as sensitive in nature and hard to transfect. Low transfection efficacies in endothelial cells limit their potential therapeutic applications. Towards improving the transfection efficiency, we made an effort to understand the internalization of lipoplexes into the cells, which is the first and most critical step in nucleic acid transfections. In this study, we demonstrated that the transient modulation of caveolae/lipid rafts mediated endocytosis with the cholesterol-sequestrating agents, nystatin, filipin III, and siRNA against Cav-1, which significantly increased the transfection properties of cationic lipid-(2-hydroxy-N-methyl-N,N-bis(2-tetradecanamidoethyl)ethanaminium chloride), namely, amide liposomes in combination with 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) (AD Liposomes) in liver sinusoidal endothelial cells (SK-Hep1). In particular, nystatin was found to be highly effective with 2–3-fold enhanced transfection efficacy when compared with amide liposomes in combination with Cholesterol (AC), by switching lipoplex internalization predominantly through clathrin-mediated endocytosis and macropinocytosis.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1420-3049/26/15/4626Test; https://doaj.org/toc/1420-3049Test

الوصول الحر: https://doaj.org/article/5c00d46f065e4f6f99ad3e3920769b82Test

المؤلفون: Priya Dharmalingam, Hari Krishna R. Rachamalla, Brijesh Lohchania, Bhanuprasad Bandlamudi, Saravanabhavan Thangavel, Mohankumar K. Murugesan, Rajkumar Banerjee, Arabinda Chaudhuri, Chandrashekhar Voshavar, Srujan Marepally

المصدر: ACS Omega, Vol 5, Iss 34, Pp 21978-21978 (2020)

مصطلحات موضوعية: Chemistry, QD1-999

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2470-1343Test

الوصول الحر: https://doaj.org/article/e598ea004a474d578f82399a83bb249eTest

المؤلفون: Priyanka Sharma, Rajkumar Banerjee, Kumar Pranav Narayan

المصدر: Data in Brief, Vol 7, Iss , Pp 428-431 (2016)

مصطلحات موضوعية: Computer applications to medicine. Medical informatics, R858-859.7, Science (General), Q1-390

الوصف: A detailed description of steroid hormone ligand containing liposomes and their stability has been given. Liposomes were complexed with β-gal DNA and used to transfect cancer and non-cancer cells. The stability of the liposomes and lipoplexes were analysed using dynamic light scattering and DNA-binding gel images. The formulations were used to assess the delivery of anticancer gene, p53 in cancer cells. The dataset consists of DNA-binding gel images, transfection, cytotoxicity and reverse transcriptase PCR images. Keywords: Steroid hormone, Liposomes, Lipoplexes, Anti-cancer gene, Transfection, Cytotoxicity

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2352340916000093Test; https://doaj.org/toc/2352-3409Test

الوصول الحر: https://doaj.org/article/aaa7533ad38f4c19b21797474f4c73dbTest

المؤلفون: Pritha Agarwalla, Rajkumar Banerjee

المصدر: Molecular Therapy: Oncolytics, Vol 3, Iss C (2016)

مصطلحات موضوعية: Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Recent study has shown that N-end rule pathway, an ubiquitin dependent proteolytic system, counteracts cell death by degrading many antisurvival protein fragments like BCLxL, BRCA1, RIPK1, etc. Inhibition of the N-end rule pathway can lead to metabolic stabilization of proapoptotic protein fragments like RIPK1, thereby sensitizing cells to programmed cell death. Receptor interacting serine-threonine protein kinase-1 (RIPK1) is one of the upstream regulators of programmed necrosis known as necroptosis. Necroptosis is particularly gaining attention of cancer biologists as it provides an alternate therapeutic modality to kill cancer cells, which often evolve multiple strategies to circumvent growth inhibition by apoptosis. Utilizing the over expression of biotin receptor in cancer cells, herein, we report that coadministration of synthetic hetero-bivalent N-end rule inhibitor RFC11 and anticancer drug shikonin solubilized in a stable biotin receptor-targeted liposome exhibited significant synergistic antitumor effect in both subcutaneous and orthotopic mouse colon tumor model through induction of necroptosis with distinctive upregulation of RIPK1. Besides developing a newly targeted formulation for necroptosis induction, this report is the first in vivo evidence demonstrating that potent inhibition of N-end rule pathway can enhance therapeutic efficacy of conventional chemotherapeutics.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S237277051730013XTest; https://doaj.org/toc/2372-7705Test

الوصول الحر: https://doaj.org/article/9d0c91a5d9cd4779adf89270367ef19aTest

المؤلفون: Yarra Venkatesh (3278346), Venugopal Vangala (4502692), Rakesh Mengji (11938011), Amrita Chaudhuri (3827266), Sayantan Bhattacharya (2246920), Prasanta Kumar Datta (4760748), Rajkumar Banerjee (1408753), Avijit Jana (1419373), N. D. Pradeep Singh (7337156)

مصطلحات موضوعية: Medicine, Physiology, Pharmacology, Biotechnology, Cancer, Space Science, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, results also showed, orthotopic murine melanoma, known anticancer drug, increased anticancer activity, color change approach, cancerous b16f10 cells, b16f10 cells indicated, 6j mouse model, co could accelerate, time monitoring ability, photochemically active single, released one equivalent, based dual co, time monitoring, one equivalent, co uncaging, vivo <, tumor volume, significant regression, releasing platform, photon uncaging, photon excitation

الوصف: Herein, we report three new metal-free, photochemically active single, dual, and combinatorial CORMs (photoCORMs) based on a carbazole-fused 1,3-dioxol-2-one moiety which released one equivalent of CO, two equivalent of CO, and a combination of one equivalent of each CO and anticancer drug upon one- and two-photon excitation, respectively. The photoCORMs exhibited good cellular uptake and real-time monitoring ability of CO uncaging by a color change approach in cancerous B16F10 cells. Interestingly, the cytotoxicity assay on B16F10 cells indicated that the dual photoCORM has increased anticancer activity over the single and combinatorial photoCORMs upon irradiation. Our results also showed that CO could accelerate the effectiveness of the well-known anticancer drug (chlorambucil). Finally, the in vivo evaluation of the dual photoCORM on an established murine melanoma tumor (C57BL/6J mouse model) manifested a significant regression of tumor volume and led to significant improvement (>50%) in the overall survivability.

العلاقة: https://figshare.com/articles/journal_contribution/One-_and_Two-Photon_Uncaging_of_Carbon_Monoxide_CO_with_Real-Time_Monitoring_On-Demand_Carbazole-Based_Dual_CO-Releasing_Platform_to_Test_over_Single_and_Combinatorial_Approaches_for_the_Efficient_Regression_of_Orthotopic_Murine_Melanoma_i_/18280611Test

الإتاحة: https://doi.org/10.1021/acs.jmedchem.1c00750.s002Test

المؤلفون: Utsav Sen, Shanooja Shanavas, Apoorva H. Nagendra, Muhammad Nihad, Debajit Chaudhury, Hari K. Rachamalla, Rajkumar Banerjee, Sudheer Shenoy P, Bipasha Bose

المصدر: Cell Biology International. 47:742-753

مصطلحات موضوعية: Cell Biology, General Medicine

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::36712c99eda8675da532e82707024795Test
https://doi.org/10.1002/cbin.11978Test