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1دورية أكاديمية
المؤلفون: Lee, Benjamin D, Neri, Uri, Roux, Simon, Wolf, Yuri I, Camargo, Antonio Pedro, Krupovic, Mart, Consortium, RNA Virus Discovery, Simmonds, Peter, Kyrpides, Nikos, Gophna, Uri, Dolja, Valerian V, Koonin, Eugene V
المصدر: Cell. 186(3)
مصطلحات موضوعية: Biological Sciences, RNA, Circular, Viroids, RNA, Catalytic, RNA, Viral, Ecosystem, Plant Diseases, RNA Virus Discovery Consortium, Medical and Health Sciences, Developmental Biology, Biological sciences, Biomedical and clinical sciences
الوصف: Viroids and viroid-like covalently closed circular (ccc) RNAs are minimal replicators that typically encode no proteins and hijack cellular enzymes for replication. The extent and diversity of viroid-like agents are poorly understood. We developed a computational pipeline to identify viroid-like cccRNAs and applied it to 5,131 metatranscriptomes and 1,344 plant transcriptomes. The search yielded 11,378 viroid-like cccRNAs spanning 4,409 species-level clusters, a 5-fold increase compared to the previously identified viroid-like elements. Within this diverse collection, we discovered numerous putative viroids, satellite RNAs, retrozymes, and ribozy-like viruses. Diverse ribozyme combinations and unusual ribozymes within the cccRNAs were identified. Self-cleaving ribozymes were identified in ambiviruses, some mito-like viruses and capsid-encoding satellite virus-like cccRNAs. The broad presence of viroid-like cccRNAs in diverse transcriptomes and ecosystems implies that their host range is far broader than currently known, and matches to CRISPR spacers suggest that some cccRNAs replicate in prokaryotes.
وصف الملف: application/pdf
الوصول الحر: https://escholarship.org/uc/item/9s10v33xTest
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2دورية أكاديمية
المؤلفون: Neri, Uri, Wolf, Yuri I, Roux, Simon, Camargo, Antonio Pedro, Lee, Benjamin, Kazlauskas, Darius, Chen, I Min, Ivanova, Natalia, Allen, Lisa Zeigler, Paez-Espino, David, Bryant, Donald A, Bhaya, Devaki, Consortium, RNA Virus Discovery, Narrowe, Adrienne B, Probst, Alexander J, Sczyrba, Alexander, Kohler, Annegret, Séguin, Armand, Shade, Ashley, Campbell, Barbara J, Lindahl, Björn D, Reese, Brandi Kiel, Roque, Breanna M, DeRito, Chris, Averill, Colin, Cullen, Daniel, Beck, David AC, Walsh, David A, Ward, David M, Wu, Dongying, Eloe-Fadrosh, Emiley, Brodie, Eoin L, Young, Erica B, Lilleskov, Erik A, Castillo, Federico J, Martin, Francis M, LeCleir, Gary R, Attwood, Graeme T, Cadillo-Quiroz, Hinsby, Simon, Holly M, Hewson, Ian, Grigoriev, Igor V, Tiedje, James M, Jansson, Janet K, Lee, Janey, VanderGheynst, Jean S, Dangl, Jeff, Bowman, Jeff S, Blanchard, Jeffrey L, Bowen, Jennifer L, Xu, Jiangbing, Banfield, Jillian F, Deming, Jody W, Kostka, Joel E, Gladden, John M, Rapp, Josephine Z, Sharpe, Joshua, McMahon, Katherine D, Treseder, Kathleen K, Bidle, Kay D, Wrighton, Kelly C, Thamatrakoln, Kimberlee, Nusslein, Klaus, Meredith, Laura K, Ramirez, Lucia, Buee, Marc, Huntemann, Marcel, Kalyuzhnaya, Marina G, Waldrop, Mark P, Sullivan, Matthew B, Schrenk, Matthew O, Hess, Matthias, Vega, Michael A, O’Malley, Michelle A, Medina, Monica, Gilbert, Naomi E, Delherbe, Nathalie, Mason, Olivia U, Dijkstra, Paul, Chuckran, Peter F, Baldrian, Petr, Constant, Philippe, Stepanauskas, Ramunas, Daly, Rebecca A, Lamendella, Regina, Gruninger, Robert J, McKay, Robert M, Hylander, Samuel, Lebeis, Sarah L, Esser, Sarah P, Acinas, Silvia G, Wilhelm, Steven S, Singer, Steven W, Tringe, Susannah S, Woyke, Tanja, Reddy, TBK, Bell, Terrence H, Mock, Thomas, McAllister, Tim, Thiel, Vera
المصدر: Cell. 185(21)
مصطلحات موضوعية: Microbiology, Biological Sciences, Bioinformatics and Computational Biology, Biotechnology, Prevention, Infectious Diseases, Genetics, Vaccine Related, 2.2 Factors relating to the physical environment, Aetiology, Infection, Bacteriophages, DNA-Directed RNA Polymerases, Genome, Viral, Phylogeny, RNA, RNA Viruses, RNA-Dependent RNA Polymerase, Virome, RNA Virus Discovery Consortium, Bactriophage, Functional protein annotation, Metatranscriptomics, RNA Virus, RNA dependent RNA polymerase, Viral Ecology, Virus, Virus - Host prediction, viral phylogeny, Medical and Health Sciences, Developmental Biology, Biological sciences, Biomedical and clinical sciences
الوصف: High-throughput RNA sequencing offers broad opportunities to explore the Earth RNA virome. Mining 5,150 diverse metatranscriptomes uncovered >2.5 million RNA virus contigs. Analysis of >330,000 RNA-dependent RNA polymerases (RdRPs) shows that this expansion corresponds to a 5-fold increase of the known RNA virus diversity. Gene content analysis revealed multiple protein domains previously not found in RNA viruses and implicated in virus-host interactions. Extended RdRP phylogeny supports the monophyly of the five established phyla and reveals two putative additional bacteriophage phyla and numerous putative additional classes and orders. The dramatically expanded phylum Lenarviricota, consisting of bacterial and related eukaryotic viruses, now accounts for a third of the RNA virome. Identification of CRISPR spacer matches and bacteriolytic proteins suggests that subsets of picobirnaviruses and partitiviruses, previously associated with eukaryotes, infect prokaryotic hosts.
وصف الملف: application/pdf
الوصول الحر: https://escholarship.org/uc/item/6d60q36gTest