المؤلفون: Rifici, Claudia, Attili, Anna-Rita, De Biase, Davide, Gonçalves Dos Santos, Roselane, Seyffert, Núbia, De Paula Castro, Thiago Luiz, Pereira Figueiredo, Henrique Cesar, Scaramozzino, Carmelo, Reale, Stefano, Paciello, Orlando, Cuteri, Vincenzo, Spier, Sharon Jane, Azevedo, Vasco, Mazzullo, Giuseppe

المصدر: Veterinary sciences. 7(2)

مصطلحات موضوعية: Corynebacterium pseudotuberculosis, Dietzia spp., Glutamicibacter creatinolyticus, bacterial myositis, horses, Veterinary Sciences

الوصف: Infectious causes of myositis are reported relatively uncommonly in horses. Among them, bacterial causes include Streptococcus equi subsp. zooepidemicus, Actinobacillus equuli, Fusobacterium spp. Staphylococcus spp, and Corynebacterium pseudotuberculosis. Infection can be spread to muscles via haematogenous or extension from skin lesions. Parasitic myositis has also been documented. In this report, a 12 year-old Italian Quarter Horse mare presented with diffuse subcutaneous nodules and masses ranging from 2 × 3 to 5 × 20 cm in size, and adherent to subcutis and muscles that were first macroscopically and cytologically diagnosed as pyogranulomas. Subsequently, histological, molecular, bacteriological, and biochemical investigations were performed. All the data obtained allowed to diagnose a severe and diffuse multibacterial granulomatous myositis caused by Corynebacterium pseudotuberculosis and Corynebacterium amycolatum. Following the therapy and an initial disappearance of most of the lesions together with a general improvement of the mare, the clinical condition deteriorated, and new nodules appeared. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and PCR techniques revealed the presence of bacteria as Glutamicibacter creatinolyticus and Dietzia spp. To the authors' knowledge, this case report represents the first description of multibacterial granulomatous myositis due to Corynebacterium pseudotuberculosis, Corynebacterium amycolatum, Glutamicibacter creatinolyticus, and Dietzia spp. in a horse reared in Italy.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/4ms9v9wdTest

المؤلفون: Santos, Roselane Gonçalves dos, Hurtado, Raquel, Rodrigues, Diego Lucas Neres, Lima, Alessandra, dos Anjos, William Ferreira, Rifici, Claudia, Attili, Anna Rita, Tiwari, Sandeep, Jaiswal, Arun Kumar, Spier, Sharon J., Mazzullo, Giuseppe, Morais-Rodrigues, Francielly, Gomide, Anne Cybelle Pinto, de Jesus, Luís Cláudio Lima, Aburjaile, Flavia Figueira, Brenig, Bertram, Cuteri, Vincenzo, Castro, Thiago Luiz de Paula, Seyffert, Núbia, Santos, Anderson, Góes-Neto, Aristóteles, de Jesus Sousa, Thiago, Azevedo, Vasco

المصدر: Research in Microbiology ; volume 174, issue 3, page 103998 ; ISSN 0923-2508

مصطلحات موضوعية: Molecular Biology, General Medicine, Microbiology

الإتاحة: https://doi.org/10.1016/j.resmic.2022.103998Test
https://api.elsevier.com/content/article/PII:S0923250822000791?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0923250822000791?httpAccept=text/plainTest

المؤلفون: Zappone, Viola, Rifici, Claudia, Marino, Matteo, Morici, Manuel, Giambrone, Giada, Spadola, Filippo

المساهمون: Zappone, Viola, Rifici, Claudia, Marino, Matteo, Morici, Manuel, Giambrone, Giada, Spadola, Filippo

العلاقة: volume:14; issue:6; firstpage:1; lastpage:8; numberofpages:8; journal:ANIMALS; https://hdl.handle.net/11570/3290128Test

الإتاحة: https://doi.org/10.3390/ani14060874Test
https://hdl.handle.net/11570/3290128Test

المؤلفون: Gonçalves dos Santos, Roselane, Castillo, Raquel Hurtado, Neres Rodrigues, Diego Lucas, Lima, Alessandra, Ferreira dos Anjos, William, Rifici, Claudia, Attili, Anna Rita, Tiwari, Sandeep, Jaiswal, Arun Kumar, Spier, Sharon J., Azevedo, Vasco

المساهمون: Gonçalves dos Santos, Roselane, Castillo, Raquel Hurtado, Neres Rodrigues, Diego Lucas, Lima, Alessandra, Ferreira dos Anjos, William, Rifici, Claudia, Attili, Anna Rita, Tiwari, Sandeep, Jaiswal, Arun Kumar, Spier, Sharon J., Azevedo, Vasco

الوصف: Dietzia strains are widely distributed in the environment, presenting an opportunistic role, and some species have undetermined taxonomic characteristics. Here, we propose the existence of errors in the classification of species in this genus using comparative genomics. We performed ANI, dDDH, pangenome and genomic plasticity analyses better to elucidate the phylogenomic relationships between Dietzia strains. For this, we used 55 genomes of Dietzia downloaded from public databases that were combined with a newly sequenced. Sequence analysis of a phylogenetic tree based on genome similarity comparisons and dDDH, ANI analyses supported grouping different Dietzia species into four distinct groups. The pangenome analysis corroborated the classification of these groups, supporting the idea that some species of Dietzia could be reassigned in a possible classification into three distinct species, each containing less variability than that found within the global pangenome of all strains. Additionally, analysis of genomic plasticity based on groups containing Dietzia strains found differences in the presence and absence of symbiotic Islands and pathogenic islands related to their isolation site. We propose that the comparison of pangenome subsets together with phylogenomic approaches can be used as an alternative for the classification and differentiation of new species of the genus Dietzia.

العلاقة: https://resolver.sub.uni-goettingen.de/purl?gro-2/117959Test; S0923250822000791

الإتاحة: https://doi.org/10.1016/j.resmic.2022.103998Test
https://resolver.sub.uni-goettingen.de/purl?gro-2/117959Test

المؤلفون: Gattuso, Debora Teresa, Polisca, Angela, Interlandi, Claudia Dina, Rizzo, Maria, Tabbì, Marco, Giudice, Elisabetta, Cristarella, Santo, Rifici, Claudia, Quartuccio, Marco, Zappone, Viola

المصدر: Frontiers in Veterinary Science; 2024, p01-08, 8p

مصطلحات موضوعية: LABORATORY dogs, DIETARY supplements, LEPIDIUM, DOGS, SEMEN analysis, SPERMATOZOA

مستخلص: Maca is a traditional Andean crop used as a nutraceutical for the fertilityenhancing properties that are linked with antioxidant activity. The aim of this study was to evaluate, for the first time, the potential beneficial effects of oral Maca (Lepidium meyenii or Lepidium peruvianum) supplementation in improving reproductive performance in male dogs. Forty-eight male dogs of different breeds were enrolled in the study, fed the same maintenance diet, and exposed to the same environmental conditions. The subjects were divided into four groups of 12 dogs each: Subfertile treatment group, Subfertile control group, Normofertile treatment group, and Normofertile control group. The dogs in the treatment groups received Lepidium meyenii in their diet in a capsule formulation [75mg/ kg Maca extract 10:1, thickening agent (hydroxypropyl methylcellulose), ground rice], while the control groups received placebo capsule (starch). For each subject included in the study, the spermiogram was analyzed at three time points of the sperm cycle: at day 0 (T0), day 31 (T31), and day 62 (T62). Dietary supplementation with Maca in subfertile subjects resulted in a significant increase in ejaculate volume and total sperm count compared to the control group. This increase was also observed in normofertile subjects in the treatment group. In addition, total and progressive motility as well as sperm morphology were significantly improved in the groups treated with Maca compared to the control groups. The results thus highlight, for the first time, the potential efficacy of supplementation with 75mg/ kg of Maca extract daily in improving semen quality in dogs. [ABSTRACT FROM AUTHOR]

: Copyright of Frontiers in Veterinary Science is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Attili, Annarita, Gonçalves dos Santos, Roselane, Hurtado Castillo, Raquel, Gabriel, Lucas, Profeta, Rodrigo, Rifici, Claudia, Mazzullo, Giuseppe, Spier Sharon, J., Pinto Gomide Anne, Cybelle, Brenig, Bertram, Gala-García, Alfonso, de Paula Castro Thiago, Luiz, Cuteri, Vincenzo, Azevedo, Vasco, Seyffert, Núbia

المساهمون: Attili, Annarita, Gonçalves dos Santos, Roselane, Hurtado Castillo, Raquel, Gabriel, Luca, Profeta, Rodrigo, Rifici, Claudia, Mazzullo, Giuseppe, Spier Sharon, J., Pinto Gomide Anne, Cybelle, Brenig, Bertram, Gala-García, Alfonso, de Paula Castro Thiago, Luiz, Cuteri, Vincenzo, Azevedo, Vasco, Seyffert, Núbia

الوصف: Glutamicibacter creatinolyticus (formerly Arthrobacter creatinolyticus) belonged to the family Microccaceae. It plays a significant role in many ecosystems: soil, water, air, cheese, plant, and it was associated to urinary tract infections and bacteremia in humans [1]. Recently, G. creatinolyticus LGCM 259 (LGCM-259) was isolated from diffuse subcutaneous nodules adherent to muscular tissues from a mare in Italy. Objectives Since the identification is only possible using 16S rRNA and MALDI- TOF sequence analyses11, this study was carried out to: -characterize LGCM-259 with a complete DNA sequence and annotation; -identify genes encoding virulence factors. Materials and methods By comparative analyses among four isolated species of different habitats, available in the NCBI database, chromosomal sequencing using Hiseq technology (Illumina, USA) was conducted. The genome of LGCM-259 was automatically annotated using PROKKA. A phylogenomic tree and the presence of virulence genes were generated by Phylogenomic Tree Tool in Pathosystems Resource Integration Center (version-3.5.17), and BLASTp against the Virulence Factor Database (VFDB), respectively. Results LGCM-259 strain was sequenced and assembled in a circular chromosome, which exhibits a length of 3.3 Mb, with a G+C content of 66.4%, and a total of 2882 CDSs, 4 clusters of rRNAS (5S, 16S, and 23S), and 61 tRNA genes, respectively. The locus tag LGCM259_1698, LGCM259_0905 and LGCM259_1698 may be involved in multiple drug resistance to Rifampin, Elfamycin, and Fluoroquinolone (rpoB, tufA, tufB). The genome also displayed copper tolerance genes (copZ, csoR_1, cutC, aniA, pcoC), resistance to heavy metals such as arsenic, cobalt-zinc-cadmium (cobT, cobS, cadA), and chrome composts which are serious environmental contaminants. Conclusion The LGCM 259 strain’s genome was first characterize and its chromosome sequence has been deposited in the NCBI database under accession number CP034412. It affects animals and carries important bacterial virulence factors ...

وصف الملف: ELETTRONICO

العلاقة: info:eu-repo/semantics/altIdentifier/isbn/9876543210; ispartofbook:Abstract Book of The 1st International Conference of the European College of Veterinary Microbiology; The 1st International Conference of the European College of Veterinary Microbiology; volume:1; firstpage:68; lastpage:68; numberofpages:1; http://hdl.handle.net/11581/434779Test; https://ebvs.eu/news/1st-international-conference-of-the-european-college-of-veterinary-microbiologyTest

الإتاحة: http://hdl.handle.net/11581/434779Test
https://ebvs.eu/news/1st-international-conference-of-the-european-college-of-veterinary-microbiologyTest

المؤلفون: Giambrone, Giada, Vullo, Cecilia, Puleio, Roberto, Rifici, Claudia, Di Giorgio, Stefania, Sfacteria, Alessandra

المصدر: Animals (2076-2615); Dec2023, Vol. 13 Issue 23, p3634, 14p

مصطلحات موضوعية: MACROPHAGES, PRUSSIAN blue, IRON, CARCINOMA, IRON metabolism, BREAST

مستخلص: Simple Summary: Macrophages play a key role in cancer. The aim of this study was to identify hemosiderin-laden macrophages (HLMs) and speculate their possible role in canine mammary carcinoma. HLMs, which were variously localized in cancer peritumoral and stromal areas, expressed CD204, an M2 macrophagic marker. In addition, they showed positivity for angiogenic and protumoral molecules, such as VEGF or TGF-α. Therefore, HLMs need to be considered for a possible role in cancer progression and survival in canine mammary tumors, protecting cells from hypoxia. Macrophages are among the main actors in cancer immunoediting, with several functions, including recycling iron and packaging it in hemosiderin. Even though TAMs are widely studied in breast cancer and canine mammary tumors, hemosiderin-laden macrophages (HLMs) have not received as much attention. Considering the growing interest in iron metabolism in cancer, this study aims to evaluate the presence of HLMs in canine mammary tumors. Fifty cases of canine mammary carcinomas presenting aggregates of pigmented macrophages were chosen. Prussian blue and Meguro staining were performed to assess the presence of iron. Immunohistochemistry was carried out to try to identify macrophagic phenotypes and hypothesize their role. Evaluation of the H&E sections showed that pigmented macrophages were variously localized in peritumoral and stromal areas. These pigmented cells were variably stained with Prussian blue and reacted strongly with DAB in the Meguro staining method, thus confirming the presence of iron within them. In their immunohistochemistry, the HLMs were negative for the MAC387 but positive for CD 204 and VEGF. Considering their positivity for CD 204, HLMs could be M2 macrophages that supply iron to both the neoplastic cells and the tumor inflammatory microenvironment, promoting angiogenesis and protecting cancer cells from hypoxia. [ABSTRACT FROM AUTHOR]

: Copyright of Animals (2076-2615) is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Attili, Anna-Rita, Iacoucci, Cristina, Serri, Evelina, Cuteri, Vincenzo, Cantalamessa, Andrea, Linardi, Martina, Rifici, Claudia, Mazzullo, Giuseppe, Rossi, Giacomo, Galosi, Livio, Tambella, Adolfo Maria

المساهمون: Università degli Studi di Camerino

المصدر: Frontiers in Veterinary Science ; volume 8 ; ISSN 2297-1769

مصطلحات موضوعية: General Veterinary

الوصف: This in vitro study was carried out to evaluate the potential antibacterial properties of canine non-transfusional hemo-components. Therapeutic formulations commonly used for regenerative medicine purposes (platelet-rich plasma, platelet gel, platelet lysate, fibrin glue), considering both leukocyte-rich and leukocyte-poor formulations, but also platelet-poor plasma and activating substances (thrombin, calcium gluconate), were tested to detect elements with potential antimicrobial properties. The antibacterial effect was tested on different bacterial strains ( Staphylococcus aureus subspecies aureus, Staphylococcus cohnii subspecies cohnii, Escherichia coli, Pseudomonas aeruginosa , and Klebsiella pneumoniae subspecies pneumoniae ) isolated from canine wounds and classified as susceptible, multidrug-, extensively, and pandrug-resistant bacteria toward a known panel of human and veterinary antibiotics. The evaluation was carried out by agar gel diffusion method (Kirby–Bauer) and micro-inhibition in broth using microplates and spectrophotometer reading. The study findings confirmed the hypothesized antibacterial properties of canine non-transfusional hemo-components. A more effective bacteriostatic effect was found against Gram-negative bacteria, drug-resistant too. The presence of leukocytes or platelets does not appear to be essential for the antibacterial effect. Further studies should be conducted to evaluate the exact mechanism of action of the antimicrobial activity. However, non-transfusional hemo-components could be a useful natural aid in controlling bacterial infections in dogs.

الإتاحة: https://doi.org/10.3389/fvets.2021.746809Test

المؤلفون: Napoli, Ettore, Sfacteria, Alessandra, Rifici, Claudia, Mazzullo, Giuseppe, Gaglio, Gabriella, Brianti, Emanuele

المساهمون: Napoli, Ettore, Sfacteria, Alessandra, Rifici, Claudia, Mazzullo, Giuseppe, Gaglio, Gabriella, Brianti, Emanuele

مصطلحات موضوعية: Aelurostrongylus abstrusu, cat lungworm, hemocyte, histology, host–parasite interaction, snail’s immune system

الوصف: Cornu aspersum, the land snail, is recognized as a suitable intermediate host of Aelurostrongylus abstrusus; however, there is little information both on larval development as well as on the intermediate host's immune system reaction to the parasite. The aim of the study was to assess the histological reaction of C. aspersum's immune system against A. abstrusus. Sixty-five snails were provided by a snail farm. Five of them were digested to assess the absence of natural parasitic infections. The remaining sixty were divided into five groups. Three groups of snails were infected with A. abstrusus using by-contact or injection methods; one group was injected only with saline solution and one group was left untreated as the control. The snails of group A were sacrificed and digested on study days 2, 10, and 18; snails of the other groups were collected and examined for histopathological analysis on study days 2, 10, and 18. On study day 2, in the infected snails, several free L1s were observed along with the absence of immune system reactions. On day 10, the L2s elicited an intense reaction in the internal layer of the muscular foot. On day 18, all L3s partially encapsulated by the snail's immune system were observed in the outermost part of the muscular foot, which is near and among the goblet cells. This last finding suggests that L3s could be shed with the snail's mucus and spread in the environment, representing an alternative route of transmission for this feline lungworm.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/37111428; volume:12; issue:4; firstpage:542; lastpage:551; numberofpages:10; journal:PATHOGENS; https://hdl.handle.net/11570/3258325Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85154598610

الإتاحة: https://doi.org/10.3390/pathogens12040542Test
https://hdl.handle.net/11570/3258325Test

المؤلفون: Quartuccio, Marco., Cristarella, Santo., Medica, Pietro., Fazio, Esterina., Mazzullo, Giuseppe., Rifici, Claudia., Liotta, Luigi., Satué Ambrojo, Katiuska.

المساهمون: UCH. Departamento de Medicina y Cirugía Animal, Producción Científica UCH 2020

مصطلحات موضوعية: Horse breeds - Jennies - Generative organs - Cytology, Estrus, Estro, Caballos - Razas - Jennies - Aparato genital - Citología

الوصف: Este artículo se encuentra disponible en la siguiente URL: https://www.mdpi.com/2076-2615/10/6/1062Test ; Este artículo pertenece al número especial "Reproduction, fertility and embryonic development of animals". ; Since in the mare and other animal species such as bitches and cats, the endometrial cell pattern varies depending on the phase of the estrous cycle, the aim of this study was to describe and quantify the endometrial cytological (EC) findings in cycling jennies. EC of eight nonpregnant jennies by cytobrush (CB) at diestrus (day 1 and day 14) and estrous (day 21) were evaluated. All slides were stained with Wright´s stain and microscopically examined at both 400 and 1000 magnification. Seven high-power fields (400 ) were assessed in each smear and the endometrial epithelial cells and neutrophils (PMNs) were counted. Endometrial epithelial cells were classified as intact, distorted or fragmented and, on the basis of the presence of dense groups, in monolayer or single clusters. Cytoplasmic characteristics, such as vacuolation or streaming and size, form, position of nuclear characteristics, including karyorrhexis, were recorded. Background aspect, as clear, proteinaceous, or debris, was also considered. In general, sampling by CB provided a yield of cells and clumped endometrial epithelial cells in many smears, being more abundant in estrus than early and late diestrus. Individual endometrial epithelial cells, during estrous, presented a columnar morphology, ciliated or not ciliated and basal nuclei. During diestrus phase, endometrial epithelial cells presented a more cuboidal ciliated or not ciliated morphology. Moderate amount of proteinacious material and red blood cells (RBC) was also observed. Non variation in the percentage of PMNs during diestrus was obtained, but lower and segmented PMNs in CB smears were shown in estrous. This study provides new insights on the physiological changes of endometrial epithelial cells in cycling jennies during the estrus cycle. The CB technique represents a suitable ...

وصف الملف: application/pdf

العلاقة: Animals, vol. 10, n. 6.; Quartuccio, M., Cristarella, S., Medica, P., Fazio, E., Mazzullo, G., Rifici, C. et al. (2020). Endometrial cytology during the different phases of the estrous cycle in Jennies: new evidences. Animals, vol. 10, i. 6 (19 jun.), art. 1062. DOI: https://doi.org/10.3390/ani10061062Test; 2076-2615 (Electrónico).; http://hdl.handle.net/10637/12497Test; https://doi.org/10.3390/ani10061062Test

الإتاحة: https://doi.org/10.3390/ani10061062Test
http://hdl.handle.net/10637/12497Test