المؤلفون: Xing-chen Zhou, Long-hao Chen, Shuang Wu, Kai-zheng Wang, Zi-cheng Wei, Tao Li, Yuan-shen Huang, Zi-han Hua, Qiong Xia, Zhi-zhen Lv, Li-jiang Lv

المصدر: BMC Complementary Medicine and Therapies, Vol 24, Iss 1, Pp 1-8 (2024)

مصطلحات موضوعية: Lumbar disc herniation, Multimodal magnetic resonance imaging, Lever positioning manipulation, Analgesia mechanism, Brain, Other systems of medicine, RZ201-999

الوصف: Abstract Introduction The clinical symptoms of Lumbar Disc Herniation (LDH) can be effectively ameliorated through Lever Positioning Manipulation (LPM), which is closely linked to the brain's pain-regulating mechanisms. Magnetic Resonance Imaging (MRI) offers an objective and visual means to study how the brain orchestrates the characteristics of analgesic effects. From the perspective of multimodal MRI, we applied functional MRI (fMRI) and Magnetic Resonance Spectrum (MRS) techniques to comprehensively evaluate the characteristics of the effects of LPM on the brain region of LDH from the aspects of brain structure, brain function and brain metabolism. This multimodal MRI technique provides a biological basis for the clinical application of LPM in LDH. Methods and analysis A total of 60 LDH patients and 30 healthy controls, matched by gender, age, and years of education, will be enrolled in this study. The LDH patients will be divided into two groups (Group 1, n = 30; Group 2, n = 30) using a random number table method. Group 1 will receive LPM treatment once every two days, for a total of 12 times over 4 weeks. Group 2 will receive sham LPM treatment during the same period as Group 1. All 30 healthy controls will be divided into Group 3. Multimodal MRI will be performed on Group 1 and Group 2 at three time points (TPs): before LPM (TP1), after one LPM session (TP2), and after a full course of LPM treatment. The healthy controls (Group 3) will not undergo LPM and will be subject to only a single multimodal MRI scan. Participants in both Group 1 and Group 2 will be required to complete clinical questionnaires. These assessments will focus on pain intensity and functional disorders, using the Visual Analog Scale (VAS) and the Japanese Orthopaedic Association (JOA) scoring systems, respectively. Discussion The purpose of this study is to investigate the multimodal brain response characteristics of LDH patients after treatment with LPM, with the goal of providing a biological basis for clinical applications. Trial registration number https://clinicaltrials.gov/ct2/show/NCT05613179Test , identifier: NCT05613179.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2662-7671Test

الوصول الحر: https://doaj.org/article/db886a5b50c1437cbe7b3c131ebfd126Test

المؤلفون: Longhao Chen, Xingchen Zhou, Chao Yang, Hong Jiao Wu, Yu Tian, Shuangwei Hong, Huijie Hu, Kaizheng Wang, Shuang Wu, Zicheng Wei, Tao Li, Yuanshen Huang, Zihan Hua, Qiong Xia, Xiao Jie Chen, Zhizhen Lv, Lijiang Lv

المصدر: Pediatric Rheumatology Online Journal, Vol 22, Iss 1, Pp 1-11 (2024)

مصطلحات موضوعية: Immune cell, Juvenile idiopathic arthritis (JIA), Mendelian randomization, Gene-wide Association, Causation analysis, Pediatrics, RJ1-570, Diseases of the musculoskeletal system, RC925-935

الوصف: Abstract Background Juvenile idiopathic arthritis (JIA) is a type of chronic childhood arthritis with complex pathogenesis. Immunological studies have shown that JIA is an acquired self-inflammatory disease, involving a variety of immune cells, and it is also affected by genetic and environmental susceptibility. However, the precise causative relationship between the phenotype of immune cells and JIA remains unclear to date. The objective of our study is to approach this inquiry from a genetic perspective, employing a method of genetic association analysis to ascertain the causal relationship between immune phenotypes and the onset of JIA. Methods In this study, a two-sample Mendelian randomization (MR) analysis was used to select single nucleotide polymorphisms (SNPs) significantly associated with immune cells as instrumental variables to analyze the bidirectional causal relationship between 731 immune cells and JIA. There were four types of immune features (median fluorescence intensity (MFI), relative cellular (RC), absolute cellular (AC), and morphological parameters (MP)). Finally, the heterogeneity and horizontal reproducibility of the results were verified by sensitivity analysis, which ensured more robust results. Results We found that CD3 on CM CD8br was causally associated with JIA at the level of 0.05 significant difference (95% CI = 0.630 ~ 0.847, P = 3.33 × 10−5, PFDR = 0.024). At the significance level of 0.20, two immunophenotypes were causally associated with JIA, namely: HLA DR on CD14+ CD16- monocyte (95% CI = 0.633 ~ 0.884, P = 6.83 × 10–4, PFDR = 0.16) and HLA DR on CD14+ monocyte (95% CI = 0.627 ~ 0.882, P = 6.9 × 10−4, PFDR = 0.16). Conclusion Our study assessed the causal effect of immune cells on JIA from a genetic perspective. These findings emphasize the complex and important role of immune cells in the pathogenesis of JIA and lay a foundation for further study of the pathogenesis of JIA.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1546-0096Test

الوصول الحر: https://doaj.org/article/00583f9ebb6b4b619911b85ac53fd3e2Test

المؤلفون: Lu-Jun Liang, Yu Wang, Xiao Hua, Rujing Yuan, Qiong Xia, Rongtian Wang, Chuntong Li, Guo-Chao Chu, Lei Liu, Yi-Ming Li

المصدر: JACS Au, Vol 3, Iss 10, Pp 2873-2882 (2023)

مصطلحات موضوعية: Chemistry, QD1-999

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2691-3704Test

الوصول الحر: https://doaj.org/article/57bb6ce5668c4a5b829625fe1ec1215bTest

المؤلفون: I. Glenn Cohen, Boris Babic, Sara Gerke, Qiong Xia, Theodoros Evgeniou, Klaus Wertenbroch

المصدر: npj Digital Medicine, Vol 6, Iss 1, Pp 1-4 (2023)

مصطلحات موضوعية: Computer applications to medicine. Medical informatics, R858-859.7

الوصف: Abstract While the literature on putting a “human in the loop” in artificial intelligence (AI) and machine learning (ML) has grown significantly, limited attention has been paid to how human expertise ought to be combined with AI/ML judgments. This design question arises because of the ubiquity and quantity of algorithmic decisions being made today in the face of widespread public reluctance to forgo human expert judgment. To resolve this conflict, we propose that human expert judges be included via appeals processes for review of algorithmic decisions. Thus, the human intervenes only in a limited number of cases and only after an initial AI/ML judgment has been made. Based on an analogy with appellate processes in judiciary decision-making, we argue that this is, in many respects, a more efficient way to divide the labor between a human and a machine. Human reviewers can add more nuanced clinical, moral, or legal reasoning, and they can consider case-specific information that is not easily quantified and, as such, not available to the AI/ML at an initial stage. In doing so, the human can serve as a crucial error correction check on the AI/ML, while retaining much of the efficiency of AI/ML’s use in the decision-making process. In this paper, we develop these widely applicable arguments while focusing primarily on examples from the use of AI/ML in medicine, including organ allocation, fertility care, and hospital readmission.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2398-6352Test

الوصول الحر: https://doaj.org/article/906055af0f4a468886aee51d5fc8e093Test

المؤلفون: Jie Zhang, Qiong Xia, Qinghua Zhang

المصدر: Clinical and Experimental Obstetrics & Gynecology, Vol 51, Iss 6, p 140 (2024)

مصطلحات موضوعية: lncrna, magi2-as3, cervical cancer, methylation, Gynecology and obstetrics, RG1-991

الوصف: Background: Cervical cancer, a malignancy of gynecological origin, typically necessitates a therapeutic approach combining surgery and chemoradiotherapy as the primary intervention. However, the 5-year survival rate remains suboptimal, prompting researchers to explore novel strategies for the early diagnosis and treatment of cervical cancer. This study delves into the investigation of human papillomavirus (HPV)-mediated DNA methylation modifications within the promoter region of the long non-coding RNA MAGI2-AS3 during cervical cancer development. This paper is an experimental study, laboratory based, using cell lines. Methods: A lentivirus overexpression vector encoding HPV16 E6/E7 proteins was established for transfecting cervical epithelial cells. The methylation status of DNA in the MAGI2-AS3 promoter region was assessed using MassARRAY, and the MAGI2-AS3 gene expression was measured through quantitative real time polymerase chain reaction (qRT-PCR). Subsequently, the correlation between methylation levels and gene expression in cervical cancer was analyzed. Results: (1) Relative to the HPV-negative cervical cancer cell line C33A, MAGI2-AS3 expression significantly decreased in the HPV-positive cervical cancer cell line Siha. (2) The methylation rate of 16 CpG sites in the HPV-positive cervical cancer cell line Siha was notably higher compared to the HPV-negative cervical cancer cell line C33A. (3) To further substantiate the regulatory impact on DNA methylation and expression within the MAGI2-AS3 promoter region, we silenced the expression of HPV16 E6 in the HPV-positive cervical cancer cell line Siha using HPV16 E6 siRNA. The ensuing qRT-PCR analysis revealed a significant up-regulation of MAGI2-AS3 expression in the HPV16 E6 siRNA group when contrasted with the negative control group. MassARRAY analysis was employed to gauge the DNA methylation levels in the promoter region of the MAGI2-AS3 gene in HPV-positive cervical cancer cells (Siha) following HPV16 E6 silencing. The results demonstrated a significantly lower methylation rate at the CpG_29 site in the HPV16 E6 siRNA group compared to the HPV16 E6 siRNA group. Conclusions: The study establishes a close association between HPV infection and elevated methylation levels coupled with diminished expression of MAGI2-AS3. The influence of HPV infection on the malignant transformation of cervical epithelial cells is potentially mediated through the regulatory modulation of MAGI2-AS3 expression via DNA methylation.

وصف الملف: electronic resource

العلاقة: https://www.imrpress.com/journal/CEOG/51/6/10.31083/j.ceog5106140Test; https://doaj.org/toc/0390-6663Test

الوصول الحر: https://doaj.org/article/fcaf846cde5f434e967e3b7e9d96e600Test

المؤلفون: Chengkun Wang, Qiong Xia, Qianhe Zhang, Yuanhao Qu, Stephen Su, Jason K. W. Cheng, Nicholas W. Hughes, Le Cong

المصدر: Frontiers in Cell and Developmental Biology, Vol 9 (2022)

مصطلحات موضوعية: CRISPR, genome editing, Cas12a, RecT, single-stranded annealing protein, Biology (General), QH301-705.5

الوصف: The development of CRISPR-based gene-editing technologies has brought an unprecedented revolution in the field of genome engineering. Cas12a, a member of the Class 2 Type V CRISPR-associated endonuclease family distinct from Cas9, has been repurposed and developed into versatile gene-editing tools with distinct PAM recognition sites and multiplexed gene targeting capability. However, with current CRISPR/Cas12a technologies, it remains a challenge to perform efficient and precise genome editing of long sequences in mammalian cells. To address this limitation, we utilized phage recombination enzymes and developed an efficient CRISPR/Cas12a tool for multiplexed precision editing in mammalian cells. Through protein engineering, we were able to recruit phage recombination proteins to Cas12a to enhance its homology-directed repair efficiencies. Our phage-recombination-assisted Cas12a system achieved up to 3-fold improvements for kilobase-scale knock-ins in human cells without compromising the specificity of the enzyme. The performance of this system compares favorably against Cas9 references, the commonly used enzyme for gene-editing tasks, with improved specificity. Additionally, we demonstrated multi-target editing with similar improved activities thanks to the RNA-processing activity of the Cas12a system. This compact, multi-target editing tool has the potential to assist in understanding multi-gene interactions. In particular, it paves the way for a gene therapy method for human diseases that complements existing tools and is suitable for polygenic disorders and diseases requiring long-sequence corrections.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fcell.2021.719705/fullTest; https://doaj.org/toc/2296-634XTest

الوصول الحر: https://doaj.org/article/7971de916c8d4e2e84fb86afdfe85239Test

المؤلفون: Zhen Liu, Chao Cheng, Xiaojun Luo, Qiong Xia, Yejie Zhang, Xiaobing Long, Qingping Jiang, Weiyi Fang

المصدر: BMC Cancer, Vol 21, Iss 1, Pp 1-1 (2021)

مصطلحات موضوعية: Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1186/s12885-016-2277-2Test.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2407Test

الوصول الحر: https://doaj.org/article/c40246f045f84e5b9f0647e3c354ce70Test

المؤلفون: Qiong Xia, Marine Saux, Maharajah Ponnaiah, Françoise Gilard, François Perreau, Stéphanie Huguet, Sandrine Balzergue, Nicolas Langlade, Christophe Bailly, Patrice Meimoun, Françoise Corbineau, Hayat El-Maarouf-Bouteau

المصدر: International Journal of Molecular Sciences, Vol 19, Iss 8, p 2464 (2018)

مصطلحات موضوعية: seed, dormancy, hormones, transcriptomics, metabolomics, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: Dormancy is an adaptive trait that blocks seed germination until the environmental conditions become favorable for subsequent vegetative plant growth. Seed dormancy is defined as the inability to germinate in favorable conditions. Dormancy is alleviated during after-ripening, a dry storage period, during which dormant (D) seeds unable to germinate become non-dormant (ND), able to germinate in a wide range of environmental conditions. The treatment of dormant seeds with ethylene (D/ET) promotes seed germination, and abscisic acid (ABA) treatment reduces non-dormant (ND/ABA) seed germination in sunflowers (Helianthus annuus). Metabolomic and transcriptomic studies have been performed during imbibition to compare germinating seeds (ND and D/ET) and low-germinating seeds (D and ND/ABA). A PCA analysis of the metabolites content showed that imbibition did not trigger a significant change during the first hours (3 and 15 h). The metabolic changes associated with germination capacity occurred at 24 h and were related to hexoses, as their content was higher in ND and D/ET and was reduced by ABA treatment. At the transcriptional level, a large number of genes were altered oppositely in germinating, compared to the low-germinating seeds. The metabolomic and transcriptomic results were integrated in the interpretation of the processes involved in germination. Our results show that ethylene treatment triggers molecular changes comparable to that of after-ripening treatment, concerning sugar metabolism and ABA signaling inhibition.

وصف الملف: electronic resource

العلاقة: http://www.mdpi.com/1422-0067/19/8/2464Test; https://doaj.org/toc/1422-0067Test

الوصول الحر: https://doaj.org/article/7017aa0389384551996c24d3725fbdc4Test

المؤلفون: Lixin Tang, Qiong Xia, Xianpeng Wang

المصدر: ISIJ International. 2016, 56(11):2006

المؤلفون: Zhang, Tu-Yan, Xia, Qiong-Xia, Yang, Xiaoyong, Zhao, Zhuang, Sun, Jiandong, Zha, Xiang-Ping, Lu, Youyue

المساهمون: Chinese Academy of Sciences, National Natural Science Foundation of China

المصدر: Ore Geology Reviews ; volume 168, page 106016 ; ISSN 0169-1368

مصطلحات موضوعية: Economic Geology, Geochemistry and Petrology, Geology

الإتاحة: https://doi.org/10.1016/j.oregeorev.2024.106016Test
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