المؤلفون: Qinhong Xu, Rutgerd Boelens, Gert Jan Veldwisch

المصدر: International Journal of the Commons, Vol 18, Iss 1, Pp 148–163-148–163 (2024)

مصطلحات موضوعية: high-efficiency irrigation, water saving, china, rural water governance, governmentality, Political institutions and public administration (General), JF20-2112

الوصف: In this paper, we investigate China’s vigorously promoted high-efficiency irrigation policies for farmland water conservation, deploying a governmentality framework. The paper explains how the modernist irrigation policies follow global discourses but seek to imbue these with new ambition and the meaning of ecological civilization. At the same time, the government aims to mold water users’ subjectivity in accordance with its development strategies. Following a local village case study, the paper further elucidates how, amidst the decline of commons’ local governance and water user responses, the state’s high-efficiency irrigation water governmentality project is adapted and negotiated. Local government bureaucracy actors and ordinary villagers challenge irrigation policies through local noncongruent institutions. Thereby, villagers’ pragmatic, non-aligned irrigation technologies and actions contradict state-assumed collective collaboration and government-aligned smooth operation.

وصف الملف: electronic resource

العلاقة: https://account.thecommonsjournal.org/index.php/up-j-ijc/article/view/1253Test; https://doaj.org/toc/1875-0281Test

الوصول الحر: https://doaj.org/article/11a3448f86be4e84968d7a8608ea3438Test

المؤلفون: Zijun Wang, Enmeng Li, Cancan Zhou, Bolun Qu, Tianli Shen, Jie Lian, Gan Li, Yiwei Ren, Yunhua Wu, Qinhong Xu, Guangbing Wei, Xuqi Li

المصدر: Journal of Investigative Surgery, Vol 36, Iss 1 (2023)

مصطلحات موضوعية: postoperative abdominal adhesions, optimized mouse models, visual observation, development process, Surgery, RD1-811

الوصف: Background: There is no clear description of the evolution of the progression of abdominal adhesions over time. Method: The optimized model was selected using different adhesion scoring systems. Then, this model was used to observe the progression of abdominal adhesions. Visualized observation of abdominal adhesion evolution was performed by laparoscopy and computed tomography. The inflammatory cell infiltration and collagen fibers in adhesion tissues at different times were evaluated by hematoxylin-eosin and picrosirius red staining. RNA sequencing was used to predict potential key targets of abdominal adhesions at different times. Results: The abdominal adhesion model showed the highest reproducibility when it was established using a circular tool and an electric brush. Based on this model, we found that the inflammatory response was activated early in the process of adhesion formation, peaking on day 3 and then gradually decreasing until stabilization on day 7. Collagen and fibronectin formed on day 1 and gradually increased until remaining stable on day 7. In addition, the characteristic changes in the adhesion zone from initial congestion, edema and fragile tissue to later dense and stable tissue could be vividly observed in live mice by laparoscopy and artificial pneumoperitoneum CT. The RNA sequencing results revealed that Hck on day 1, Ndufs3 and Ndufs8 on day 3 and Aif1 on day 7 might play key roles in abdominal adhesion formation. Conclusion: The construction of a standard process for describing the evolution of abdominal adhesions based on an optimized mouse model will help to facilitate subsequent adhesion-related studies.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/0894-1939Test; https://doaj.org/toc/1521-0553Test

الوصول الحر: https://doaj.org/article/e013d138f912438aa2a3809850576252Test

المؤلفون: Ganghua Yang, Zhengyang Lu, Fandi Meng, Yong Wan, Lei Zhang, Qinhong Xu, Zheng Wang

المصدر: Scientific Reports, Vol 12, Iss 1, Pp 1-10 (2022)

مصطلحات موضوعية: Medicine, Science

الوصف: Abstract MicroRNA-141(miR-141) has been reported to play vital roles in the regulation of carcinogenesis and cancer progression. However, the biological function of miR-141 in GBC has received less attention. The aim of this study was to estimate the potential value of the expression level of miR-141 as a diagnostic and prognostic blood-based biomarker in gallbladder cancer (GBC) patients. Meanwhile, to explore its biological role in GBC cells. RT-PCR was employed to confirm the expression of miR-141 in ten paired tissue samples (10 GBC tissues and 10 adjacent normal gallbladder tissues), GBC cell lines and peripheral blood specimens from 98 GBC patients and 60 healthy controls. MTT assay was used to evaluate the GBC cells proliferation and flow cytometry was used to detect the cell apoptosis. Receiver operating characteristic curve analysis and the area under the curve (AUC) were used to evaluate the value of miR-141 plasma levels for GBC diagnosis. Finally, clinicopathological and survival data of all GBC patients were collected and analyzed. Here, we confirmed that the expression of miR-141 were upregulated in primary gallbladder cancer cells and tissues compared with human gallbladder epithelial cells and adjacent normal tissues (P

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/202352a48b644a149ac866e7b141b8deTest

المؤلفون: Chao Cao, Feng Qiu, Chengcheng Lou, Lingling Fang, Fang Liu, Jingjing Zhong, Weijie Sun, Weiping Ding, Xiaopin Yu, Qinhong Xu, Ran Wang, Liemin Ruan, Qifa Song

المصدر: Respiratory Research, Vol 23, Iss 1, Pp 1-8 (2022)

مصطلحات موضوعية: SARS-CoV-2, Vaccine, Allergic diseases, Safety, Immunogenicity, Diseases of the respiratory system, RC705-779

الوصف: Abstract Background Considering the considerable prevalence of allergic disease in the general population, an urgent need exists for inactivated SARS-CoV-2 vaccines that can be safely administered to those subjects. Methods This retrospective cohort study including 1926 participants who received inactivated SARS-CoV-2 vaccines, compared their local and systemic reactions in 7 days after each dose of inactivated SARS-CoV-2 vaccine, and anti–SARS-CoV-2 IgG after vaccination in all participants. Results Pain at the injection site within seven days after the first injection was the most commonly reported local reaction, occurring in 31.0% of the patients with allergic disease and 18.9% in the control group, respectively (P

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1465-993XTest

الوصول الحر: https://doaj.org/article/6c7ca45aeceb4b94943bf14f0c1dfa10Test

المؤلفون: Tao Qin, Liang Cheng, Ying Xiao, Weikun Qian, Jie Li, Zheng Wu, Zheng Wang, Qinhong Xu, Wanxing Duan, Lucas Wong, Erxi Wu, Qingyong Ma, Jiguang Ma

المصدر: Frontiers in Oncology, Vol 10 (2020)

مصطلحات موضوعية: resveratrol, NAF-1, cancer stem cells, pancreatic cancer, progression, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Resveratrol is a natural polyphenolic compound with multiple biological effects, e.g., proliferation inhibition, anti-oxidation, and neuroprotection. Besides that, studies have shown that resveratrol inhibits tumor growth and migration, as well as epithelial–mesenchymal transition (EMT). However, its molecular mechanisms in tumor progression are not fully understood. Nutrient-deprivation autophagy factor-1 (NAF-1) is mainly found in the endoplasmic reticulum and mitochondrial outer membrane. It is an important genetic locus for regulating oxidative stress and autophagy. The molecular mechanism of NAF-1 in pancreatic cancer is currently unclear. The current study found that NAF-1 is expressed in pancreatic cancer tissue and correlated with the progression of pancreatic cancer. Furthermore, we found that NAF-1 inhibition significantly inhibits the stem cell characteristics and the invasion and migration abilities of pancreatic cancer cells. In a subcutaneous xenograft model of pancreatic cancer in nude mice, resveratrol inhibited the expression of NAF-1, thereby inhibiting tumor growth. Taken together, resveratrol could be an effective anti-tumor drug, and NAF-1 may be a rational therapeutic target.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/article/10.3389/fonc.2020.01038/fullTest; https://doaj.org/toc/2234-943XTest

الوصول الحر: https://doaj.org/article/bb92346a9a5b44fd8a3cdd3ad9c46c2eTest

المؤلفون: Ying Xiao, Tao Qin, Liankang Sun, Weikun Qian, Jie Li, Wanxing Duan, Jianjun Lei, Zheng Wang, Jiguang Ma, Xuqi Li, Qingyong Ma, Qinhong Xu

المصدر: Cell Transplantation, Vol 29 (2020)

مصطلحات موضوعية: Medicine

الوصف: Pancreatic cancer is characterized by a hypoxic tumor microenvironment, which is primarily caused by massive fibrosis with pancreatic stellate cells (PSCs) as a main component. Our previous studies have shown that resveratrol can significantly inhibit pancreatic cancer. However, whether resveratrol can inhibit hypoxia-induced cancer development remains unclear. The objective of this study was to explore whether PSCs and hypoxia synergistically mediate aggressiveness in pancreatic cancer and detect the potential pleiotropic protective effects of resveratrol on hypoxia-induced pancreatic cancer progression. Human PSCs were treated with vehicle or resveratrol under normoxic or hypoxic conditions (3% O 2 ), and PSC activation was assessed by immunofluorescence staining. SiRNA was used to silence hypoxia-inducible factor 1 (HIF-1) expression. The invasive capacity of Panc-1 and Mia Paca-2 cells cocultured with conditioned medium from PSCs was assessed by Transwell assays. To examine tumor formation kinetics, KPC (LSL-Kras G12D/+ , Trp53 fl/+ , and Pdx1-Cre) mice were sacrificed at different time points. To investigate the antitumor effects of resveratrol in vivo, 8-wk-old KPC mice were divided into two groups and treated daily with or without 50 mg/kg resveratrol. Our data indicate that hypoxia induces PSC activation via HIF-1 and that the interleukin 6, vascular endothelial growth factor A, and stromal cell-derived factor 1 derived from activated PSCs promote both invasion and the epithelial–mesenchymal transition and inhibit apoptosis in pancreatic cancer cells. However, resveratrol inhibits hypoxia-induced PSC activation, blocks the interplay between PSCs and pancreatic cancer cells, and suppresses the malignant progression of pancreatic cancer and stromal desmoplasia in a KPC mouse model. Our data highlight that activated PSCs and intratumoral hypoxia are essential targets for novel strategies to prevent tumor–microenvironment interactions. Furthermore, the polyphenolic compound resveratrol effectively ameliorates the malignant progression of pancreatic ductal adenocarcinoma.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1555-3892Test

الوصول الحر: https://doaj.org/article/aedb8b03726249de9c1b86135fb1c41bTest

المؤلفون: Zhengdong Jiang, Cancan Zhou, Liang Cheng, Bin Yan, Ke Chen, Xin Chen, Liang Zong, Jianjun Lei, Wanxing Duan, Qinhong Xu, Xuqi Li, Zheng Wang, Qingyong Ma, Jiguang Ma

المصدر: Journal of Experimental & Clinical Cancer Research, Vol 37, Iss 1, Pp 1-14 (2018)

مصطلحات موضوعية: YAP, Pancreatic cancer, Pancreatic stellate cells, Invasion, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Abstract Background Hippo/YAP pathway is known to be important for development, growth and organogenesis, and dysregulation of this pathway leads to tumor progression. We and others find that YAP is up-regulated in pancreatic ductal adenocarcinoma (PDAC) and associated with worse prognosis of patients. Activated pancreatic stellate cells (PSCs) forming the components of microenvironment that enhance pancreatic cancer cells (PCs) invasiveness and malignance. However, the role and mechanism of YAP in PDAC tumor-stromal interaction is largely unknown. Methods The expression of YAP in Pancreatic cancer cell lines and PDAC samples was examined by Western blot and IHC. The biological role of YAP on cancer cell proliferation, epithelial-mesenchymal transition (EMT) and invasion were evaluated by MTT, Quantitative real-time PCR analysis, Western blot analysis and invasion assay. The effect of YAP on PSC activation was evaluated by PC-PSC co-culture conditions and xenograft PDAC mouse model. Results Firstly, knockdown of YAP inhibits PDAC cell proliferation and invasion in vitro. In addition, YAP modulates the PC and PSC interaction via reducing the production of connective tissue growth factor (CTGF) from PCs, inhibits paracrine-mediated PSC activation under PC-PSC co-culture conditions and in turn disrupts TGF-β1-mediated tumor-stromal interactions. Lastly, inhibiting YAP expression prevents tumor growth and suppresses desmoplastic reaction in vivo. Conclusions These results demonstrate that YAP contributes to the proliferation and invasion of PC and the activation of PSC via tumor-stromal interactions and that targeting YAP may be a promising therapeutic strategy for PDAC treatment.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s13046-018-0740-4Test; https://doaj.org/toc/1756-9966Test

الوصول الحر: https://doaj.org/article/af6fb7324ff54faaad79ff7fd5afbac9Test

المؤلفون: Wei Li, Han Liu, Weikun Qian, Liang Cheng, Bin Yan, Liang Han, Qinhong Xu, Qingyong Ma, Jiguang Ma

المصدر: Computational and Structural Biotechnology Journal, Vol 16, Iss , Pp 479-487 (2018)

مصطلحات موضوعية: Biotechnology, TP248.13-248.65

الوصف: Background: Diabetes mellitus and pancreatic cancer are intimately related. Our previous studies showed that high levels of blood glucose promote epithelial-mesenchymal transition of pancreatic cancer. In this study, we evaluated the relationship between hyperglycemia and hypoxic tumor microenvironments. Methods: HIF-1α expression was evaluated by immunohistochemistry in clinical pancreatic cancer tissues with or without diabetes mellitus. Statistcal analysis was performed to explore the relationship between HIF-1α expression and pathological features of patients with pancreatic cancer. In vivo and in vitro models was established to detect whether a hyperglycemia environment could cause hypoxia in the pancreatic parenchyma and promote pancreatic cancer. In addition, we also tested the effect of HIF-1α siRNA on the high glucose-induced invasive and migratory abilities of BxPC-3 cells in culture. Result: Our data showed that pancreatic cancer patients with diabetes had a higher level of HIF-1α expression as well as biliary duct invasion and larger tumor volumes than individuals in the euglycemic group. Diabetic nude mice treated with streptozotocin (STZ) exhibited larger tumors and were more likely to develop liver metastasis than control mice. Acinar cells of the pancreas in diabetic mice showed an obvious expansion of the endoplasmic reticulum and increased nuclear gaps as well as chromatin close to the cellular membrane in some acinar cells. The expression area for Hypoxyprobe-1 and HIF-1α in the diabetic orthotopic xenograft group was larger than that in the control group. The expression level of HIF-1α in the BxPC-3 cancer cell line increased in response to high glucose and CoCl2 concentrations. The high glucose-induced invasive ability, migratory capacity and MMP-9 expression were counter-balanced by siRNA specific to HIF-1α. Conclusion: Our results demonstrate that the association between hyperglycemia and poor prognosis can be attributed to microenvironment hypoxia in pancreatic cancer. Keywords: Hyperglycemia, Hypoxia, HIF-1α, Metastasis, Pancreatic cancer

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2001037018301120Test; https://doaj.org/toc/2001-0370Test

الوصول الحر: https://doaj.org/article/a17f97b90d3e49a792eae4d12d711604Test

المؤلفون: Liang Zong, Ke Chen, Zhengdong Jiang, Xin Chen, Liankang Sun, Jiguang Ma, Cancan Zhou, Qinhong Xu, Wanxing Duan, Liang Han, Jianjun Lei, Xuqi Li, Qingyong Ma, Zheng Wang

المصدر: Journal of Experimental & Clinical Cancer Research, Vol 36, Iss 1, Pp 1-12 (2017)

مصطلحات موضوعية: Pancreatic cancer, Lipoxin A4, Mesenchymal phenotypes, TGF-β1, Invasion and metastasis, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Abstract Background Pancreatic cancer is a lethal disease in part because of its potential for aggressive invasion and metastasis. Lipoxin A4 (LXA4) is one of the metabolites that is derived from arachidonic acid and that is catalyzed by 15-lipoxygenase (15-LOX), and it has recently been reported to exhibit anti-cancer effects. However, the role of LXA4 in pancreatic cancer remains to be elucidated. Methods Pancreatic cell lines were treated with vehicle or LXA4, and the invasive capacity was then assessed by Transwell assays. The expression of epithelial and mesenchymal markers was determined by western blotting and immunofluorescence. Anti-TGF-β1 neutralizing antibody and exogenous recombinant human TGF-β1 (rhTGF-β1) were used to study the effect of LXA4 on the TGF-β signaling. A liver metastasis model was applied to investigate the effect of LXA4 in vivo. The correlation between the Lipoxin effect score (LES) and the clinical-pathological features of pancreatic cancer was also analyzed. Results We found that in patients with pancreatic cancer, low LES was correlated with aggressive metastatic potential. The LXA4 activity, which was mediated by the LXA4 receptor FPRL1, could significantly suppress invasion capacity and mesenchymal phenotypes. The expression and autocrine signaling pathway activity of TGF-β1 were also downregulated by LXA4. In the liver metastasis model in nude mice, the stable analog of LXA4, BML-111, could inhibit the metastasis of pancreatic cancer cells. Conclusion Our results demonstrated that LXA4 could reverse mesenchymal phenotypes, which attenuated invasion and metastasis via the inhibition of autocrine TGF-β1 signaling in pancreatic cancer, which may provide a new strategy to prevent the metastasis of pancreatic cancer.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s13046-017-0655-5Test; https://doaj.org/toc/1756-9966Test

الوصول الحر: https://doaj.org/article/b24364895500424b998dd2da5b8810c0Test

المؤلفون: Fang Hao, Qinhong Xu, Jing Wang, Shuo Yu, Hui-Hua Chang, James Sinnett-Smith, Guido Eibl, Enrique Rozengurt

المصدر: PLoS ONE, Vol 14, Iss 5, p e0216603 (2019)

مصطلحات موضوعية: Medicine, Science

الوصف: We examined the impact of statins on Yes-associated Protein (YAP) localization, phosphorylation and transcriptional activity in human and mouse pancreatic ductal adenocarcinoma (PDAC) cells. Exposure of sparse cultures of PANC-1 and MiaPaCa-2 cells to cerivastatin or simvastatin induced a striking re-localization of YAP from the nucleus to the cytoplasm and inhibited the expression of the YAP/TEAD-regulated genes Connective Tissue Growth Factor (CTGF) and Cysteine-rich angiogenic inducer 61 (CYR61). Statins also prevented YAP nuclear import and expression of CTGF and CYR61 stimulated by the mitogenic combination of insulin and neurotensin in dense culture of these PDAC cells. Cerivastatin, simvastatin, atorvastatin and fluvastatin also inhibited colony formation by PANC-1 and MiaPaCa-2 cells in a dose-dependent manner. In contrast, the hydrophilic statin pravastatin did not exert any inhibitory effect even at a high concentration (10 μM). Mechanistically, cerivastatin did not alter the phosphorylation of YAP at Ser127 in either PANC-1 or MiaPaCa-2 cells incubated without or with neurotensin and insulin but blunted the assembly of actin stress fiber in these cells. We extended these findings with human PDAC cells using primary KC and KPC cells, (expressing KrasG12D or both KrasG12D and mutant p53, respectively) isolated from KC or KPC mice. Using cultures of these murine cells, we show that lipophilic statins induced striking YAP translocation from the nucleus to the cytoplasm, inhibited the expression of Ctgf, Cyr61 and Birc5 and profoundly inhibited colony formation of these cells. Administration of simvastatin to KC mice subjected to diet-induced obesity prevented early pancreatic acini depletion and PanIN formation. Collectively, our results show that lipophilic statins restrain YAP activity and proliferation in pancreatic cancer cell models in vitro and attenuates early lesions leading to PDAC in vivo.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1932-6203Test

الوصول الحر: https://doaj.org/article/fa5a88971cf444c8bf64d60253d7c445Test