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1دورية أكاديمية
المؤلفون: Yuzhen Qian, Yixuan Sun, Peishang Shi, Xiuman Zhou, Qiongqiong Zhang, Qingyu Dong, Shengzhe Jin, Lu Qiu, Xiaoshuang Niu, Xiaowen Zhou, Wenshan Zhao, Yahong Wu, Wenjie Zhai, Yanfeng Gao
المصدر: Acta Pharmaceutica Sinica B, Vol 14, Iss 3, Pp 1150-1165 (2024)
مصطلحات موضوعية: LAG-3, FGL1, PD-1, PD-L1, Peptide, Immune checkpoint, Therapeutics. Pharmacology, RM1-950
الوصف: Aside from antibodies, peptides show great potential as immune checkpoint inhibitors (ICIs) due to several advantages, such as better tumor penetration and lower cost. Lymphocyte-activation gene 3 (LAG-3) is an immune checkpoint which can induce T cell dysfunction through interaction with its soluble ligand fibrinogen like protein-1 (FGL1). Here, we found that LAG-3 expression was higher than programmed cell death protein 1 (PD-1) in multiple human cancers by TCGA databases, and successfully identified a LAG-3 binding peptide LFP-6 by phage display bio-panning, which specifically blocks the interaction of LAG-3/FGL1 but not LAG-3/MHC-II. Subsequently, d-amino acids were introduced to substitute the N- and C-terminus of LFP-6 to obtain the proteolysis-resistant peptide LFP-D1, which restores T cell function in vitro and inhibits tumor growth in vivo. Further, a bispecific peptide LFOP targeting both PD-1/PD-L1 and LAG-3/FGL1 was designed by conjugating LFP-D1 with PD-1/PD-L1 blocking peptide OPBP-1(8–12), which activates T cell with enhanced proliferation and IFN-γ production. More importantly, LFOP combined with radiotherapy significantly improve the T cell infiltration in tumor and elevate systemic antitumor immune response. In conclusion, we developed a novel peptide blocking LAG-3/FGL1 which can restore T cell function, and the bispecific peptide synergizes with radiotherapy to further enhance the antitumor immune response.
وصف الملف: electronic resource
العلاقة: http://www.sciencedirect.com/science/article/pii/S221138352300480XTest; https://doaj.org/toc/2211-3835Test
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2دورية أكاديمية
المؤلفون: Lingfei Tang, Bowen Chen, Zhonghan Zhang, Changqi Ma, Junchao Chen, Yage Huang, Fengrui Zhang, Qingyu Dong, Guoyong Xue, Daiqian Chen, Chenji Hu, Shuzhou Li, Zheng Liu, Yanbin Shen, Qi Chen, Liwei Chen
المصدر: Nature Communications, Vol 14, Iss 1, Pp 1-10 (2023)
مصطلحات موضوعية: Science
الوصف: Abstract Solid polymer electrolytes (SPEs), which are favorable to form intimate interfacial contacts with electrodes, are promising electrolyte of choice for long-cycling lithium metal batteries (LMBs). However, typical SPEs with easily oxidized oxygen-bearing polar groups exhibit narrow electrochemical stability window (ESW), making it impractical to increase specific capacity and energy density of SPE based LMBs with charging cut-off voltage of 4.5 V or higher. Here, we apply a polyfluorinated crosslinker to enhance oxidation resistance of SPEs. The crosslinked network facilitates transmission of the inductive electron-withdrawing effect of polyfluorinated segments. As a result, polyfluorinated crosslinked SPE exhibits a wide ESW, and the Li|SPE|LiNi0.5Co0.2Mn0.3O2 cell with a cutoff voltage of 4.5 V delivers a high discharge specific capacity of ~164.19 mAh g−1 at 0.5 C and capacity retention of ~90% after 200 cycles. This work opens a direction in developing SPEs for long-cycling high-voltage LMBs by using polyfluorinated crosslinking strategy.
وصف الملف: electronic resource
العلاقة: https://doaj.org/toc/2041-1723Test
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3دورية أكاديمية
المؤلفون: Xiuman Zhou, Chunxia Chen, Guanyu Chen, Wenjie Zhai, Yahong Wu, Yanfeng Gao, Xinghua Sui, Wenhui Shen, Peishang Shi, Qingyu Dong, Wentong Tian, Xueqin Zhu, Xiaoxi Wang, Shengzhe Jin, Lu Qiu
المصدر: Journal for ImmunoTherapy of Cancer, Vol 11, Iss 6 (2023)
مصطلحات موضوعية: Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
الوصف: Background Aside from immune checkpoint inhibitors targeting programmed cell death protein 1 (PD-1) and programmed death ligand 1 (PD-L1), intervention of CD47/Sirpα mediated ‘don’t eat me’ signal between macrophage and tumor cell is considered as a promising therapeutic approach for cancer immunotherapy. Compared with CD47, the novel immune checkpoint CD24/Siglec-10 can also deliver ‘don’t eat me’ signal and CD24 shows much lower expression level in normal tissue which might avoid unwanted side effects.Methods Cell-based phage display biopanning and D-amino acid modification strategy were used to identify the CD24/Siglec-10 blocking peptide. Cell-based blocking assay and microscale thermophoresis assay were used to validate the blocking and binding activities of the peptide. Phagocytosis and co-culture assays were used to explore the in vitro function of the peptide. Flow cytometry was performed to assess the immune microenvironment after the peptide treatment in vivo.Results A CD24/Siglec-10 blocking peptide (CSBP) with hydrolysis-resistant property was identified. Surprisingly, we found that CSBP could not only block the interaction of CD24/Siglec-10 but also PD-1/PD-L1. CSBP could induce the phagocytosis of tumor cell by both the macrophages and monocytic myeloid-derived suppressor cells (M-MDSCs), which can further activate CD8+ T cells. Besides, combination of radiotherapy and CSBP synergistically reduced tumor growth and altered the tumor microenvironment in both anti-PD-1-responsive MC38 and anti-PD-1-resistant 4T1 tumor models.Conclusions In summary, this is the first CD24/Siglec-10 blocking peptide which blocked PD-1/PD-L1 interaction as well, functioned via enhancing the phagocytosis of tumor cells by macrophages and M-MDSCs, and elevating the activity of CD8+ T cells for cancer immunotherapy.
وصف الملف: electronic resource
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4دورية أكاديمية
المؤلفون: Wei Gu, Guoyong Xue, Qingyu Dong, Ruowei Yi, Yayun Mao, Lei Zheng, Haikuo Zhang, Xiulin Fan, Yanbin Shen, Liwei Chen
المصدر: eScience, Vol 2, Iss 5, Pp 486-493 (2022)
مصطلحات موضوعية: Ni-rich layered oxides, Lithium-ion batteries, Interface analysis, Electrolyte additives, Cathode electrolyte interphase, Mechanical engineering and machinery, TJ1-1570, Electronics, TK7800-8360
الوصف: Ni-rich layered oxides are attractive cathode materials for advanced lithium-ion batteries (LIBs) due to their high energy density. However, their large-scale application is seriously hindered by their interfacial instability, especially at a high cut-off potential. Here, we demonstrate that trimethoxyboroxine (TMOBX) is an effective film-forming additive to address the interfacial instability of LiNi0.8Co0.1Mn0.1O2 (NCM811) material at a high cut-off voltage of 4.5 V. We find that TMOBX decomposes before carbonate solvent and forms a thin cathode electrolyte interphase (CEI) layer on the surface of the NCM811 material. This TMOBX-formed CEI significantly suppresses electrolyte decomposition at a high potential and inhibits the dissolution of transition metals from NCM811 during cycling. In addition, electron-deficient borate compounds coordinate with anions (PF6−, F−, etc.) and H2O in the battery, further improving the battery's stability. As a result, adding 1.0 wt% of TMOBX boosts the capacity retention of a Li||NCM811 cell from 68.72% to 86.60% after 200 cycles at 0.5C in the range of 2.8–4.5 V.
وصف الملف: electronic resource
العلاقة: http://www.sciencedirect.com/science/article/pii/S2667141722000611Test; https://doaj.org/toc/2667-1417Test
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5دورية أكاديمية
المؤلفون: Ding Yuan, Wei Zhou, Ting Fu, Qingyu Dong
المصدر: Chinese Journal of Mechanical Engineering, Vol 34, Iss 1, Pp 1-11 (2021)
مصطلحات موضوعية: Microchannel, Connected grooves, Heat transfer enhancement, Performance evaluation, Ocean engineering, TC1501-1800, Mechanical engineering and machinery, TJ1-1570
الوصف: Abstract To improve the heat transfer performance of microchannels, a novel microchannel embedded with connected grooves crossing two sidewalls and the bottom surface (type A) was designed. A comparative study of heat transfer was conducted regarding the performances of type A microchannels, microchannels embedded with grooves on their bottom (including types B and C), or on the sidewalls (type D) as well as smooth rectangular microchannels (type E) via a three-dimensional numerical simulation and experimental validation (at Reynolds numbers from 118 to 430). Numerical results suggested that the average Nusselt number of types A, B, C, and D microchannels were 106, 73.4, 50.1, and 12.6% higher than that of type E microchannel, respectively. The smallest synergy angle β and entropy generation number N s,a were determined for type A microchannels based on field synergy and nondimensional entropy analysis, which indicated that type A exhibited the best heat transfer performance. Numerical flow analysis indicated that connected grooves induced fluid to flow along two different temperature gradients, which contributed to enhanced heat transfer performance.
وصف الملف: electronic resource
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6دورية أكاديمية
المؤلفون: Xiuman Zhou, Ling Jiao, Yuzhen Qian, Qingyu Dong, Yixuan Sun, Wei V. Zheng, Wenshan Zhao, Wenjie Zhai, Lu Qiu, Yahong Wu, Hongfei Wang, Yanfeng Gao, Junhui Chen
المصدر: Biomolecules, Vol 11, Iss 5, p 706 (2021)
مصطلحات موضوعية: CD47/SIRPα, TIGIT/PVR, drug-repositioning, small molecule inhibitor, azelnidipine, cancer immunotherapy, Microbiology, QR1-502
الوصف: Strategies boosting both innate and adaptive immunity have great application prospects in cancer immunotherapy. Antibodies dual blocking the innate checkpoint CD47 and adaptive checkpoint PD-L1 or TIGIT could achieve durable anti-tumor effects. However, a small molecule dual blockade of CD47/SIRPα and TIGIT/PVR pathways has not been investigated. Here, an elevated expression of CD47 and PVR was observed in tumor tissues and cell lines analyzed with the GEO datasets and by flow cytometry, respectively. Compounds approved by the FDA were screened with the software MOE by docking to the potential binding pockets of SIRPα and PVR identified with the corresponding structural analysis. The candidate compounds were screened by blocking and MST binding assays. Azelnidipine was found to dual block CD47/SIRPα and TIGIT/PVR pathways by co-targeting SIRPα and PVR. In vitro, azelnidipine could enhance the macrophage phagocytosis when co-cultured with tumor cells. In vivo, azelnidipine alone or combined with irradiation could significantly inhibit the growth of MC38 tumors. Azelnidipine also significantly inhibits the growth of CT26 tumors, by enhancing the infiltration and function of CD8+ T cell in tumor and systematic immune response in the tumor-draining lymph node and spleen in a CD8+ T cell dependent manner. Our research suggests that the anti-hypertensive drug azelnidipine could be repositioned for cancer immunotherapy.
وصف الملف: electronic resource
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7دورية أكاديمية
المؤلفون: Qing Ji, Weiheng Chen, Xiaoping Chen, Xiaoyan Wang, Qingyu Dong, Shanshan Yin, Yanbin Shen, Peter Müller-Buschbaum, Ya-Jun Cheng, Yonggao Xia
مصطلحات موضوعية: Biophysics, Medicine, Biotechnology, Hematology, Space Science, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, reasonable redox potential, 01 – 3, high specific capacity, considerably high capacity, although four phases, ion intercalation kinetics, high specific performance, 5 , 2 , 0 – 3, ion intercalation, situ <, electrochemical performance, two phases, xrd measurements, synergistic effect, stable lifetime, single phase, reversible lithium, property enhancement, phase concept, niobium pentoxides, monoclinic phase
الوصف: Niobium pentoxides (Nb 2 O 5 ) present great potential as next-generation anode candidates due to exceptional lithium-ion intercalation kinetics, considerably high capacity, and reasonable redox potential. Although four phases of Nb 2 O 5 including hexagonal, orthorhombic, tetragonal, and monoclinic polymorphs show diverse characteristics in electrochemical performance, stable lifetime, high specific capacity, and fast intercalation properties cannot be delivered simultaneously with a single phase. Herein, this issue is addressed by generating a homogeneous mixture of orthorhombic and monoclinic crystals at the nanoscale. Reversible lithium-ion intercalation/deintercalation of the monoclinic phase is achieved, and exceptional lithium storage sites are created at the interface of the two phases. As a result, electrochemical features of stable lifetime from the orthorhombic phase and high specific performance from the monoclinic phase are harmoniously combined. This dual-phase Nb 2 O 5 /C nanohybrids deliver as high as 380 mA h g –1 (0.01–3.0 V) and 184 mA h g –1 (1.0–3.0 V) after 200 cycles. The essential principle of property enhancement is further confirmed through in situ XRD measurements and DFT calculations. The dual-phase concept can be further applied on electrodes with multiphases to achieve high electrochemical performance.
الإتاحة: https://doi.org/10.1021/acsami.3c17230.s001Test
https://figshare.com/articles/journal_contribution/Synergistic_Effect_of_Dual_Phases_to_Improve_Lithium_Storage_Properties_of_Nb_sub_2_sub_O_sub_5_sub_/25131317Test -
8دورية أكاديمية
المؤلفون: Zhehong Ai, Longhan Zhang, Yangguan Chen, Yifan Long, Boyuan Li, Qingyu Dong, Yueming Wang, Jing Jiang
مصطلحات موضوعية: Biophysics, Medicine, Biotechnology, Science Policy, Space Science, Mathematical Sciences not elsewhere classified, wide dynamic range, satisfying multiple figures, customized multiobjective functions, best material globally, art detection limit, best ingredient composition, aware chemical descriptors, method could achieve, demand research objectives, demand materials synthesis, vast design space, demand optimization, search space, optimal composition, functional materials, aware algorithm, novel method, work demonstrates, traditional one, time methods, thereby achieving, study engineered, sensing array, research developed
الوصف: Synthesizing the best material globally is challenging; it needs to know what and how much the best ingredient composition should be for satisfying multiple figures of merit simultaneously. Traditional one-variable-at-a-time methods are inefficient; the design-build-test-learn (DBTL) method could achieve the optimal composition from only a handful of ingredients. A vast design space needs to be explored to discover the possible global optimal composition for on-demand materials synthesis. This research developed a hypothesis-guided DBTL (H-DBTL) method combined with robots to expand the dimensions of the search space, thereby achieving a better global optimal performance. First, this study engineered the search space with knowledge-aware chemical descriptors and customized multiobjective functions to fulfill on-demand research objectives. To verify this concept, this novel method was used to optimize colorimetric ammonia sensors across a vast design space of as high as 19 variables, achieving two remarkable optimization goals within 1 week: first, a sensing array was developed for ammonia quantification of a wide dynamic range, from 0.5 to 500 ppm; second, a new state-of-the-art detection limit of 50 ppb was reached. This work demonstrates that the H-DBTL approach, combined with a robot, develops a novel paradigm for the on-demand optimization of functional materials.
الإتاحة: https://doi.org/10.1021/acssensors.3c02043.s001Test
https://figshare.com/articles/journal_contribution/On-Demand_Optimization_of_Colorimetric_Gas_Sensors_Using_a_Knowledge-Aware_Algorithm-Driven_Robotic_Experimental_Platform/25194264Test -
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المؤلفون: Daiqian Chen, Chenji Hu, Qi Chen, Guoyong Xue, Lingfei Tang, Qingyu Dong, Bowen Chen, Fengrui Zhang, Mingwen Gao, Jingjing Xu, Yanbin Shen, Liwei Chen
المصدر: Nano Research. 16:3847-3854
مصطلحات موضوعية: General Materials Science, Electrical and Electronic Engineering, Condensed Matter Physics, Atomic and Molecular Physics, and Optics
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::91d03286e12504cff8401f4e3226c2a4Test
https://doi.org/10.1007/s12274-022-4845-xTest -
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المؤلفون: Xinyu Ma, Jiangtao Yu, Qingyu Dong, Xiuyang Zou, Lei Zheng, Yin Hu, Yanbin Shen, Liwei Chen, Feng Yan
المصدر: ACS Applied Materials & Interfaces. 14:41103-41113
مصطلحات موضوعية: General Materials Science
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::18154fe2564f9ec69d981d90ed4ea290Test
https://doi.org/10.1021/acsami.2c12497Test
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