-
1دورية أكاديمية
المؤلفون: Bell, RJ, Hakeem, AR, Pandanaboyana, S, Davidson, BR, Prasad, RK, Dasari, BVM
المصدر: BJS Open , 6 (6) , Article zrac131. (2022)
الوصف: BACKGROUND: This meta-analysis aimed to compare progression to surgery, extent of liver hypertrophy, and postoperative outcomes in patients planned for major hepatectomy following either portal vein embolization (PVE) or dual vein embolization (DVE) for management of an inadequate future liver remnant (FLR). METHODS: An electronic search was performed of MEDLINE, Embase, and PubMed databases using both medical subject headings (MeSH) and truncated word searches. Articles comparing PVE with DVE up to January 2022 were included. Articles comparing sequential DVE were excluded. ORs, risk ratios, and mean difference (MD) were calculated using fixed and random-effects models for meta-analysis. RESULTS: Eight retrospective studies including 523 patients were included in the study. Baseline characteristics between the groups, specifically, age, sex, BMI, indication for resection, and baseline FLR (ml and per cent) were comparable. The percentage increase in hypertrophy was larger in the DVE group, 66 per cent in the DVE group versus 27 per cent in the PVE group, MD 39.07 (9.09, 69.05) (P = 0.010). Significantly fewer patients failed to progress to surgery in the DVE group than the PVE group, 13 per cent versus 25 per cent respectively OR 0.53 (0.31, 0.90) (P = 0.020). Rates of post-hepatectomy liver failure 13 per cent versus 22 per cent (P = 0.130) and major complications 20 per cent versus 28 per cent (Clavien–Dindo more than IIIa) (P = 0.280) were lower. Perioperative mortality was lower with DVE, 1 per cent versus 10 per cent (P = 0.010). CONCLUSION: DVE seems to produce a greater degree of hypertrophy of the FLR than PVE alone which translates into more patients progressing to surgery. Higher quality studies are needed to confirm these results.
وصف الملف: application/pdf
العلاقة: https://discovery.ucl.ac.uk/id/eprint/10163392/1/zrac131.pdfTest; https://discovery.ucl.ac.uk/id/eprint/10163392Test/
-
2دورية أكاديمية
المؤلفون: Tiede, C, Bedford, R, Heseltine, S, Smith, G, Wijetunga, I, Ross, R, AlQallaf, D, Roberts, APE, Balls, A, Curd, A, Hughes, RE, Martin, H, Needham, SR, Zanetti-Domingues, LC, Sadigh, Y, Peacock, TP, Tang, AA, Gibson, N, Kyle, H, Platt, GW, Ingram, N, Taylor, T, Coletta, LP, Manfield, I, Knowles, M, Bell, S, Esteves, F, Maqbool, A, Prasad, RK, Drinkhill, M, Bon, RS, Patel, V, Goodchild, SA, Martin-Fernandez, M, Owens, RJ, Nettleship, JE, Webb, ME, Harrison, M, Lippiat, JD, Ponnambalam, S, Peckham, M, Smith, A, Ferrigno, PK, Johnson, M, McPherson, MJ, Tomlinson, DC
الوصف: Molecular recognition reagents are key tools for understanding biological processes and are used universally by scientists to study protein expression, localisation and interactions. Antibodies remain the most widely used of such reagents and many show excellent performance, although some are poorly characterised or have stability or batch variability issues, supporting the use of alternative binding proteins as complementary reagents for many applications. Here we report on the use of Affimer proteins as research reagents. We selected 12 diverse molecular targets for Affimer selection to exemplify their use in common molecular and cellular applications including the (a) selection against various target molecules; (b) modulation of protein function in vitro and in vivo; (c) labelling of tumour antigens in mouse models; and (d) use in affinity fluorescence and super-resolution microscopy. This work shows that Affimer proteins, as is the case for other alternative binding scaffolds, represent complementary affinity reagents to antibodies for various molecular and cell biology applications.
العلاقة: https://ora.ox.ac.uk/objects/uuid:fcf56628-19a1-4b5e-af7c-f8e5da55b375Test; https://doi.org/10.7554/eLife.24903Test
الإتاحة: https://doi.org/10.7554/eLife.24903Test
https://ora.ox.ac.uk/objects/uuid:fcf56628-19a1-4b5e-af7c-f8e5da55b375Test -
3دورية أكاديمية
المصدر: Indian Journal of Dental Research ; volume 26, issue 1, page 21 ; ISSN 0970-9290
-
4دورية أكاديمية
المؤلفون: Augustin, M.J, Ramesh, V, Prasad, RK, Gupta, N, Kumar, MR
مصطلحات موضوعية: Aircraft Design, Testing & Performance, Aircraft Stability and Control, Composite Materials, Metals and Metallic Materials
الوصف: Among different manufacturing techniques available for composite aircraft structures, prepreg-based manual layup is widely used. During the fabrication process, the protective films of the prepregs or other materials used in the process could get inside as a foreign object between the layers. The present method of finding the inclusions during the prepreg layup is by visual inspection in the cleanroom. Carrying out visual inspection is challenging as the layup is usually carried out on large surfaces and reflective by nature. This paper proposes a 3D laser scanner-based approach for the detection of inclusion on flat and curved surfaces. Using the portable laser scanner, the surfaces of each layer are scanned and compared the resulting point clouds using with a reference layer data. Thicknesses between two surfaces are computed with Cloud to Cloud, Mesh to Cloud and Hausdorff distance to enhance the visibility of inclusions. It was found that this approach could enhance the visibility of inclusions over 50 micron and above. These enhanced features are used to train a multiview convolutional neural network to mark the inclusion regions, which can aid the inspector to identify the inclusion regions in a fast and efficient way.
العلاقة: Augustin, M.J and Ramesh, V and Prasad, RK and Gupta, N and Kumar, MR (2021) Detection of inclusion by using 3D laser scanner in composite prepreg manufacturing technique using convolutional neural networks. Machine Vision and Applications, 32 (6). ISSN 09328092
الإتاحة: https://nal-ir.nal.res.in/13514Test/
-
5دورية أكاديمية
المؤلفون: Kumar, Vipin, Prasad, RK, Suman, SN, Dash, BP
المصدر: ORYZA-An International Journal of Rice; Vol. 56 No. 3 (2019) ; 2249-5266 ; 0474-7615
الوصف: The results of green house experiment indicated that the yield of root, shoot and total dry matter of rice increased from 0.18 to 0.23 g pot-1, from 1.49 to 1.87 g pot-1 and from 1.67 to 2.09 g pot-1, respectively with increasing levels of Cd-FA (fulvic acid). The dry matter yields, concentrations and uptake of Cd by rice were significantly and positively correlated with organic carbon and DTPA-extractable Cd in soil. Root density and root length of rice exhibited positive and significant correlation with organic carbon, CEC, EC and negative correlation with clay content of the soil. On the other hand, root radius and half distance between roots showed significant and negative correlation with these parameters. DTPA-extractable Cd increased with increasing levels of Cd-FA application in soils. A regression model developed was found to predict the uptake of Cd on the basis of root parameters (root radius, root length, root density, half distance between roots) and diffusion coefficient of Cd in soils with almost 95 % level of confidence.
وصف الملف: application/pdf
-
6دورية أكاديمية
المؤلفون: Webster, PJ, Littlejohns, AT, Gaunt, HJ, Young, RS, Rode, B, Ritchie, JE, Stead, LF, Harrison, S, Droop, A, Martin, HL, Tomlinson, DC, Hyman, A, Appleby, HL, Boxall, S, Bruns, AF, Li, J, Prasad, RK, Lodge, JPA, Burke, DA, Beech, DJ
الوصف: Surgical resection of colorectal cancer liver metastases (CLM) can be curative, yet 80% of patients are unsuitable for this treatment. As angiogenesis is a determinant of CLM progression we isolated endothelial cells from CLM and sought a mechanism which is upregulated, essential for angiogenic properties of these cells and relevant to emerging therapeutic options. Matched CLM endothelial cells (CLMECs) and endothelial cells of normal adjacent liver (LiECs) were superficially similar but transcriptome sequencing revealed molecular differences, one of which was unexpected upregulation and functional significance of the checkpoint kinase WEE1. Western blotting confirmed that WEE1 protein was upregulated in CLMECs. Knockdown of WEE1 by targeted short interfering RNA or the WEE1 inhibitor AZD1775 suppressed proliferation and migration of CLMECs. Investigation of the underlying mechanism suggested induction of double-stranded DNA breaks due to nucleotide shortage which then led to caspase 3-dependent apoptosis. The implication for CLMEC tube formation was striking with AZD1775 inhibiting tube branch points by 83%. WEE1 inhibitors might therefore be a therapeutic option for CLM and could be considered more broadly as anti-angiogenic agents in cancer treatment.
وصف الملف: text
العلاقة: https://eprints.whiterose.ac.uk/110510/14/15039-222966-4-PB.pdfTest; Webster, PJ, Littlejohns, AT, Gaunt, HJ et al. (17 more authors) (2017) Upregulated WEE1 protects endothelial cells of colorectal cancer liver metastases. Oncotarget, 8 (26). pp. 42288-42299. ISSN 1949-2553
-
7دورية أكاديمية
المؤلفون: Tiede C, Bedford R, Heseltine SJ, Smith G, Wijetunga I, Ross R, AlQallaf D, Roberts AP, Balls A, Curd A, Hughes RE, Martin H, Needham SR, Zanetti-Domingues LC, Sadigh Y, Peacock TP, Tang AA, Gibson N, Kyle H, Platt GW, Ingram N, Taylor T, Coletta LP, Manfield I, Knowles M, Bell S, Esteves F, Maqbool A, Prasad RK, Drinkhill M, Bon RS, Patel V, Goodchild SA, Martin-Fernandez M, Owens RJ, Nettleship JE, Webb ME, Harrison M, Lippiat JD, Ponnambalam S, Peckham M, Smith A, Ferrigno PK, Johnson M, McPherson MJ, Tomlinson DC
العلاقة: urn:ISSN:2050-084X; eLife; http://purl.org/net/epubs/series/40758527Test; Central Laser Facility (1977- ); CLF - Science-Lasers for Science; Science and Technology Facilities Council (2007- ); http://purl.org/net/epubs/work/34080684Test
-
8
المصدر: International Journal of Medical and Clinical Research. 3:190-194
مصطلحات موضوعية: medicine.medical_specialty, Hematology, business.industry, Internal medicine, Medicine, Medical physics, business
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::3f1add96d6f88bcb1381b805d0dc9606Test
https://doi.org/10.9735/0976-5530.3.6.190-194Test -
9دورية أكاديمية
المؤلفون: Krivov, SV, Fenton, H, Goldsmith, PJ, Prasad, RK, Fisher, J, Paci, E
الوصف: The evolution of disease or the progress of recovery of a patient is a complex process, which depends on many factors. A quantitative description of this process in real-time by a single, clinically measurable parameter (biomarker) would be helpful for early, informed and targeted treatment. Organ transplantation is an eminent case in which the evolution of the post-operative clinical condition is highly dependent on the individual case. The quality of management and monitoring of patients after kidney transplant often determines the long-term outcome of the graft. Using NMR spectra of blood samples, taken at different time points from just before to a week after surgery, we have shown that a biomarker can be found that quantitatively monitors the evolution of a clinical condition. We demonstrate that this is possible if the dynamics of the process is considered explicitly: the biomarker is defined and determined as an optimal reaction coordinate that provides a quantitatively accurate description of the stochastic recovery dynamics. The method, originally developed for the analysis of protein folding dynamics, is rigorous, robust and general, i.e., it can be applied in principle to analyze any type of biological dynamics. Such predictive biomarkers will promote improvement of long-term graft survival after renal transplantation, and have potentially unlimited applications as diagnostic tools.
وصف الملف: text
العلاقة: https://eprints.whiterose.ac.uk/79641/1/journal.pcbi.1003685.pdfTest; Krivov, SV, Fenton, H, Goldsmith, PJ et al. (3 more authors) (2014) Optimal reaction coordinate as a biomarker for the dynamics of recovery from kidney transplant. PLoS Computational Biology, 10 (6). e1003685. ISSN 1553-734X
الإتاحة: https://doi.org/10.1371/journal.pcbi.1003685Test
https://eprints.whiterose.ac.uk/79641Test/
https://eprints.whiterose.ac.uk/79641/1/journal.pcbi.1003685.pdfTest -
10دورية أكاديمية
المؤلفون: SAKSENA, S, SINGH, PB, PRASAD, RK, PRASAD, R, MALHOTRA, P, JOSHI, V, PATIL, RS
مصطلحات موضوعية: Developing-Countries, India, Environment, Combustion, Particles, Smoke, Pm10, Exposure Assessment, Indoor Air Quality, Outdoor Air Quality, Respirable Particles, Carbon Monoxide, Infants, Women, Urban Areas, envir, archi
الوصف: Indoor air pollution is potentially a very serious environmental and public health problem in India. In poor communities, with the continuing trend in biofuel combustion coupled with deteriorating housing conditions, the problem will remain for some time to come. While to some extent the problem has been studied in rural areas, there is a dearth of reliable data and knowledge about the situation in urban slum areas. The microenvironmental model was used for assessing daily-integrated exposure of infants and women to respirable suspended particulates (RSP) in two slums of Delhi-one in an area of high outdoor pollution and the other in a less polluted area. The study confirmed that indoor concentrations of RSP during cooking in kerosene-using houses are lesser than that in wood-using houses. However, the exposure due to cooking was not significantly different across the two groups. This was because, perhaps due to socioeconomic reasons, kerosene-using women were found to cook for longer durations, cook inside more often, and that infants in such houses stayed in the kitchen for longer durations. It was observed that indoor background levels during the day and at nighttime can be exceedingly high. We speculate that this may have been due to resuspension of dust, infiltration, unknown sources, or a combination of these factors. The outdoor RSP levels measured just outside the houses ( near ambient) were not correlated with indoor background levels and were higher than those reported by the ambient air quality monitoring network at the corresponding stations. More importantly, the outdoor levels measured in this study not only underestimated the daily-integrated exposure, but were also poorly correlated with it.
النص الكامل