المؤلفون: Alisjahbana, Dewi H., Nurmawati, Syndi, Milanti, Mia, Djauhari, Hofiya, Ledermann, Jeremy P., Antonjaya, Ungke, Dewi, Yora Permata, Johar, Edison, Wiyatno, Ageng, Sriyani, Ida Yus, Alisjahbana, Bachti, Safari, Dodi, Myint, Khin Saw Aye, Powers, Ann M., Hakim, Dzulfikar DL

المساهمون: Brookes, Victoria J., Universitas Padjadjaran, Centers for Disease Control and Prevention

المصدر: PLOS Neglected Tropical Diseases ; volume 17, issue 11, page e0011769 ; ISSN 1935-2735

الوصف: Central nervous system (CNS) viral infections are critical causes of morbidity and mortality in children; however, comprehensive data on etiology is lacking in developing countries such as Indonesia. To study the etiology of CNS infections in a pediatric population, 50 children admitted to two hospitals in Bandung, West Java, during 2017–2018 were enrolled in a CNS infection study. Cerebrospinal fluid and serum specimens were tested using molecular, serological, and virus isolation platforms for a number of viral and bacteriological agents. Causal pathogens were identified in 10 out of 50 (20%) and included cytomegalovirus (n = 4), Streptococcus pneumoniae (n = 2), tuberculosis (n = 2), Salmonella serotype Typhi (n = 1) and dengue virus (n = 1). Our study highlights the importance of using a wide range of molecular and serological detection methods to identify CNS pathogens, as well as the challenges of establishing the etiology of CNS infections in pediatric populations of countries with limited laboratory capacity.

الإتاحة: https://doi.org/10.1371/journal.pntd.0011769Test

المؤلفون: Byers, Nathaniel M., Burns, Paul L., Stuchlik, Olga, Reed, Matthew S., Ledermann, Jeremy P., Pohl, Jan, Powers, Ann M.

المساهمون: Samy, Abdallah M., American Society for Microbiology, Oak Ridge Institute for Science and Education

المصدر: PLOS Neglected Tropical Diseases ; volume 17, issue 1, page e0011028 ; ISSN 1935-2735

الوصف: Chikungunya virus (CHIKV) and the closely related onyong-nyong virus (ONNV) are arthritogenic arboviruses that have caused significant, often debilitating, disease in millions of people. However, despite their kinship, they are vectored by different mosquito subfamilies that diverged 180 million years ago (anopheline versus culicine subfamilies). Previous work indicated that the nonstructural protein 3 (nsP3) of these alphaviruses was partially responsible for this vector specificity. To better understand the cellular components controlling alphavirus vector specificity, a cell culture model system of the anopheline restriction of CHIKV was developed along with a protein expression strategy. Mosquito proteins that differentially interacted with CHIKV nsP3 or ONNV nsP3 were identified. Six proteins were identified that specifically bound ONNV nsP3, ten that bound CHIKV nsP3 and eight that interacted with both. In addition to identifying novel factors that may play a role in virus/vector processing, these lists included host proteins that have been previously implicated as contributing to alphavirus replication.

الإتاحة: https://doi.org/10.1371/journal.pntd.0011028Test

المؤلفون: Ledermann, Jeremy P., Burns, Paul L., Perinet, Lara C., Powers, Ann M., Byers, Nathaniel M.

المصدر: Vector-Borne and Zoonotic Diseases ; volume 24, issue 1, page 55-63 ; ISSN 1530-3667 1557-7759

مصطلحات موضوعية: Virology, Infectious Diseases, Microbiology

الإتاحة: https://doi.org/10.1089/vbz.2022.0093Test

المؤلفون: Bettis, Alison A., L’Azou Jackson, Maïna, Yoon, In-Kyu, Breugelmans, J. Gabrielle, Goios, Ana, Gubler, Duane J., Powers, Ann M.

المساهمون: Vasconcelos, Pedro F. C., CEPI, the European Union’s Horizon 2020 research and innovation programme

المصدر: PLOS Neglected Tropical Diseases ; volume 16, issue 1, page e0010069 ; ISSN 1935-2735

الوصف: Chikungunya fever is an acute febrile illness that is often associated with severe polyarthralgia in humans. The disease is caused by chikungunya virus (CHIKV), a mosquito-borne alphavirus. Since its reemergence in 2004, the virus has spread throughout the tropical world and several subtropical areas affecting millions of people to become a global public health issue. Given the significant disease burden, there is a need for medical countermeasures and several vaccine candidates are in clinical development. To characterize the global epidemiology of chikungunya and inform vaccine development, we undertook a systematic literature review in MEDLINE and additional public domain sources published up to June 13, 2020 and assessed epidemiological trends from 1999 to 2020. Observational studies addressing CHIKV epidemiology were included and studies not reporting primary data were excluded. Only descriptive analyses were conducted. Of 3,883 relevant sources identified, 371 were eligible for inclusion. 46% of the included studies were published after 2016. Ninety-seven outbreak reports from 45 countries and 50 seroprevalence studies from 31 countries were retrieved, including from Africa, Asia, Oceania, the Americas, and Europe. Several countries reported multiple outbreaks, but these were sporadic and unpredictable. Substantial gaps in epidemiological knowledge were identified, specifically granular data on disease incidence and age-specific infection rates. The retrieved studies revealed a diversity of methodologies and study designs, reflecting a lack of standardized procedures used to characterize this disease. Nevertheless, available epidemiological data emphasized the challenges to conduct vaccine efficacy trials due to disease unpredictability. A better understanding of chikungunya disease dynamics with appropriate granularity and better insights into the duration of long-term population immunity is critical to assist in the planning and success of vaccine development efforts pre and post licensure.

الإتاحة: https://doi.org/10.1371/journal.pntd.0010069Test

المؤلفون: Powers, Ann M.

المصدر: Clinical Infectious Diseases, 2018 Aug . 67(4), 622-623.

الوصول الحر: https://www.jstor.org/stable/26524992Test

المؤلفون: Stubbs, Samuel C. B., Johar, Edison, Yudhaputri, Frilasita A., Yohan, Benediktus, Santoso, Marsha S., Hayati, Rahma F., Denis, Dionisius, Blacklaws, Barbara A., Powers, Ann M., Sasmono, R. Tedjo, Myint, Khin Saw Aye, Frost, Simon D. W.

مصطلحات موضوعية: Research Article, Biology and life sciences, Medicine and health sciences, People and places, Research and analysis methods, Computer and information sciences

الوصف: Funder: US-CDC ; Background: Chikungunya virus (CHIKV) is an important emerging and re-emerging public health problem worldwide. In Indonesia, where the virus is endemic, epidemiological information from outside of the main islands of Java and Bali is limited. Methodology/Principal Findings: Four hundred and seventy nine acutely febrile patients presenting between September 2017–2019 were recruited from three city hospitals situated in Ambon, Maluku; Banjarmasin, Kalimantan; and Batam, Batam Island as part of a multi-site observational study. CHIKV RNA was detected in a single serum sample while a separate sample was IgM positive. IgG seroprevalence was also low across all three sites, ranging from 1.4–3.2%. The single RT-PCR positive sample from this study and 24 archived samples collected during other recent outbreaks throughout Indonesia were subjected to complete coding region sequencing to assess the genetic diversity of Indonesian strains. Phylogenetic analysis revealed all to be of a single clade, which was distinct from CHIKV strains recently reported from neighbouring regions including the Philippines and the Pacific Islands. Conclusions/Significance: Chikungunya virus strains from recent outbreaks across Indonesia all belong to a single clade. However, low-level seroprevalence and molecular detection of CHIKV across the three study sites appears to contrast with the generally high seroprevalences that have been reported for non-outbreak settings in Java and Bali, and may account for the relative lack of CHIKV epidemiological data from other regions of Indonesia.

وصف الملف: application/zip; application/pdf; text/xml

العلاقة: https://www.repository.cam.ac.uk/handle/1810/315759Test

الإتاحة: https://doi.org/10.17863/CAM.62873Test
https://www.repository.cam.ac.uk/handle/1810/315759Test

المؤلفون: Alisjahbana, Dewi Hawani, Nurmawati, Syndi, Hakim, Dzulfikar DL, Milanti, Mia, Dewi, Yora Permata, Johar, Edison, Myint, Khin Saw Aye, Lederman, Jeremy P, Powers, Ann M, Alisjahbana, Bachti, Antonjaya, Ungke

المصدر: SAGE Open Medical Case Reports ; volume 9, page 2050313X2110343 ; ISSN 2050-313X 2050-313X

مصطلحات موضوعية: General Medicine

الوصف: Central nervous system involvement of dengue virus is increasingly reported from endemic areas. This study describes the clinical characteristics and laboratory features of a pediatric patient enrolled in a central nervous system illness study conducted in 2017–2018 to identify viral and bacterial etiologies in Indonesian children. Dengue diagnostics including molecular and serological testing were performed on an encephalitis patient who presented with both classical dengue and neurological clinical symptoms. Dengue virus serotype 1 RNA was detected in both cerebrospinal fluid and serum by serotype-specific reverse transcription polymerase chain reaction, and the E gene was successfully sequenced. Anti-dengue virus immunoglobulin M was detected in both admission and discharge sera, whereas anti-dengue virus immunoglobulin G was identified only in the discharge serum. This study describes the central nervous system complications in a case with dengue virus infection in West Java, Indonesia, and highlights the potential for dengue virus serotype 1, a serotype rarely associated with neurotropism, to cause encephalitis.

الإتاحة: https://doi.org/10.1177/2050313x211034393Test

المؤلفون: Powers, Ann M

المساهمون: Andreadis, Theodore

المصدر: Journal of Medical Entomology ; volume 59, issue 1, page 20-26 ; ISSN 0022-2585 1938-2928

مصطلحات موضوعية: Infectious Diseases, Insect Science, General Veterinary, Parasitology

الوصف: Eastern equine encephalitis virus (EEEV; Family Togaviridae), is an endemic pathogen first isolated in 1933 with distribution primarily in the eastern US and Canada. The virus has caused periodic outbreaks in both humans and equines along the eastern seaboard and through the southern coastal states. While the outbreaks caused by EEEV have been sporadic and varied geographically since the discovery of the virus, it has continued to expand its range moving into the Midwest states as well. Additionally, one of the largest outbreaks was recorded in 2019 prompting concerns that outbreaks were becoming larger and more frequent. Because the virus can cause serious disease and because it is transmissible by both mosquitoes and aerosol, there has been renewed interest in identifying potential options for vaccines. Currently, there are no licensed vaccines and control relies completely on the use of personal protective measures and integrated vector control which have limited effectiveness for the EEEV vectors. Several vaccine candidates are currently being developed; this review will describe the multiple options under consideration for future development and assess their relative advantages and disadvantages.

الإتاحة: https://doi.org/10.1093/jme/tjab135Test
https://academic.oup.com/jme/article-pdf/59/1/20/41961386/tjab135.pdfTest

المؤلفون: Staples, J. Erin, Powers, Ann M.

المصدر: Principles and Practice of Pediatric Infectious Diseases ; page 1145-1147.e3 ; ISBN 9780323756082

الإتاحة: https://doi.org/10.1016/b978-0-323-75608-2.00217-2Test
https://api.elsevier.com/content/article/PII:B9780323756082002172?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:B9780323756082002172?httpAccept=text/plainTest

المؤلفون: Stubbs, Samuel CB, Johar, Edison, Yudhaputri, Frilasita A, Yohan, Benediktus, Santoso, Marsha S, Hayati, Rahma F, Denis, Dionisius, Blacklaws, Barbara A, Powers, Ann M, Sasmono, R Tedjo, Myint, Khin Saw Aye, Frost, Simon DW

مصطلحات موضوعية: Adolescent, Adult, Chikungunya Fever, Chikungunya virus, Child, Preschool, Disease Outbreaks, Female, Humans, Indonesia, Infant, Male, Middle Aged, Phylogeny, RNA, Viral, Seroepidemiologic Studies, Young Adult

الوصف: BACKGROUND: Chikungunya virus (CHIKV) is an important emerging and re-emerging public health problem worldwide. In Indonesia, where the virus is endemic, epidemiological information from outside of the main islands of Java and Bali is limited. METHODOLOGY/PRINCIPAL FINDINGS: Four hundred and seventy nine acutely febrile patients presenting between September 2017-2019 were recruited from three city hospitals situated in Ambon, Maluku; Banjarmasin, Kalimantan; and Batam, Batam Island as part of a multi-site observational study. CHIKV RNA was detected in a single serum sample while a separate sample was IgM positive. IgG seroprevalence was also low across all three sites, ranging from 1.4-3.2%. The single RT-PCR positive sample from this study and 24 archived samples collected during other recent outbreaks throughout Indonesia were subjected to complete coding region sequencing to assess the genetic diversity of Indonesian strains. Phylogenetic analysis revealed all to be of a single clade, which was distinct from CHIKV strains recently reported from neighbouring regions including the Philippines and the Pacific Islands. CONCLUSIONS/SIGNIFICANCE: Chikungunya virus strains from recent outbreaks across Indonesia all belong to a single clade. However, low-level seroprevalence and molecular detection of CHIKV across the three study sites appears to contrast with the generally high seroprevalences that have been reported for non-outbreak settings in Java and Bali, and may account for the relative lack of CHIKV epidemiological data from other regions of Indonesia. ; UK Medical Research Council (MR/P017541/1) held by SDWF and the Indonesian Science Fund (DIPI) and Indonesia Endowment Fund for Education (LPDP) held by RTS (MRCUK6) as part of the UK-Indonesia Joint Health Research Call on Infectious Diseases (2016). SDWF was supported in part by The Alan Turing Institute via an Engineering and Physical Sciences Research Council grant (EP/510129/1)

وصف الملف: Electronic-eCollection; image/tiff; application/octet-stream; application/pdf

العلاقة: https://www.repository.cam.ac.uk/handle/1810/312349Test

الإتاحة: https://doi.org/10.17863/CAM.59441Test
https://www.repository.cam.ac.uk/handle/1810/312349Test