المؤلفون: Shaily Chauhan, Seekha Naik, Rohit Kumar, Janne Ruokolainen, Kavindra Kumar Kesari, Monalisa Mishra, Piyush Kumar Gupta

المصدر: ACS Omega, Vol 9, Iss 6, Pp 6549-6555 (2024)

مصطلحات موضوعية: Chemistry, QD1-999

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2470-1343Test

الوصول الحر: https://doaj.org/article/b0de7da6eab2447bb6808796df2d7eeeTest

المؤلفون: Sumel Ashique, Neeraj Mishra, Ashish Garg, Belay Zeleke Sibuh, Pankaj Taneja, Gopal Rai, Sinouvassane Djearamane, Ling Shing Wong, Noura Al-Dayan, Shatabhisha Roychoudhury, Kavindra Kumar Kesari, Petr Slama, Shubhadeep Roychoudhury, Piyush Kumar Gupta

المصدر: Frontiers in Nutrition, Vol 10 (2023)

مصطلحات موضوعية: ulcerative colitis, synbiotics, gut microbiota, inflammation, oxidative stress, superoxide dismutase, Nutrition. Foods and food supply, TX341-641

الوصف: Ulcerative colitis (UC) is presently considered a multifactorial pathology, which may lead to persistent inflammatory action of the gastrointestinal tract (GIT) because of an improperly managed immunological reactivity to the intestinal microbiota found in the GIT. The immune response to common commensal microbes plays an essential role in intestinal inflammation related to UC synbiotics, and it is an important element in the optimal therapy of UC. Therefore, synbiotics, i.e., a mixture of prebiotics and probiotics, may help control the diseased state. Synbiotics alleviate the inflammation of the colon by lowering the reactive oxygen species (ROS) and improving the level of antioxidant enzymes such as catalase (CAT), glutathione peroxidase (GPX), and superoxide dismutase (SOD). Prebiotic supplementation is not a common practice at the moment, despite numerous research findings proving that the benefits of both probiotics and prebiotics encourage their continued existence and positioning in the GIT, with positive effects on human health by managing the inflammatory response. However, the fact that there have been fewer studies on the treatment of UC with different probiotics coupled with selected prebiotics, i.e., synbiotics, and the outcomes of these studies have been very favorable. This evidence-based study explores the possible role of ROS, SOD, and synbiotics in managing the UC. The proposed review also focuses on the role of alteration of gut microbiota, antioxidant defense in the gastrointestinal tract, and the management of UC. Thus, the current article emphasizes oxidative stress signaling in the GI tract, oxidative stress-based pathomechanisms in UC patients, and UC therapies inhibiting oxidative stress’ effects.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fnut.2023.1126579/fullTest; https://doaj.org/toc/2296-861XTest

الوصول الحر: https://doaj.org/article/94059f7daae643de86d62845b2160cb2Test

المؤلفون: Hem C. Joshi, Himanshu Kharkwal, Ajay Kumar, Piyush Kumar Gupta

المصدر: Sensors International, Vol 4, Iss , Pp 100248- (2023)

مصطلحات موضوعية: Quartz crystal microbalance, Flow injection assay, Mesothelin, Cancer diagnosis, Immunosensor, Electrical engineering. Electronics. Nuclear engineering, TK1-9971

الوصف: A robust real-time flow injection assay using Quartz Crystal Microbalance (QCM) biosensor has been developed for the detection of mesothelin, an antigen expressed on various malignant tumors including mesothelioma and ovarian cancers. A QCM sensor chip functionalized with self-assembled monolayer of cysteamine used to fabricate a sensitive immunosensor. Mesothelin specific antibody was immobilized on cysteamine modified gold surface of quartz crystal using N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide and N-hydroxy succinimide coupling. Further, ethanolamine was used as a blocking reagent to prevent the non-specific adsorption on antibody functionalized gold surface. Various concentrations of mesothelin was tested and the resonant frequency variation of the crystal was observed by a quartz crystal microbalance until a stable response is obtained. A good correlation between the frequency changes and concentration of mesothelin tested was observed and the QCM biosensor detects mesothelin in the linear range of 100pg/mLto 50 ng/mL. Thus, QCM assay could be a promising technique for early diagnosis of mesothelioma, ovarian and pancreatic adenocarcinoma.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2666351123000220Test; https://doaj.org/toc/2666-3511Test

الوصول الحر: https://doaj.org/article/33465d9ffa45417eb2101a2e79fa54b4Test

المؤلفون: Sibi Raj, Kavindra Kumar Kesari, Arun Kumar, Brijesh Rathi, Ashok Sharma, Piyush Kumar Gupta, Saurabh Kumar Jha, Niraj Kumar Jha, Petr Slama, Shubhadeep Roychoudhury, Dhruv Kumar

المصدر: Molecular Cancer, Vol 21, Iss 1, Pp 1-16 (2022)

مصطلحات موضوعية: Head and neck squamous cell carcinoma, C-MET, EGFR, Hepatocyte growth factor, Chemoresistance, Monoclonal antibody, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Abstract Head and neck cancer is the sixth most common cancer across the globe. This is generally associated with tobacco and alcohol consumption. Cancer in the pharynx majorly arises through human papillomavirus (HPV) infection, thus classifying head and neck squamous cell carcinoma (HNSCC) into HPV-positive and HPV-negative HNSCCs. Aberrant, mesenchymal-epithelial transition factor (c-MET) signal transduction favors HNSCC progression by stimulating proliferation, motility, invasiveness, morphogenesis, and angiogenesis. c-MET upregulation can be found in the majority of head and neck squamous cell carcinomas. c-MET pathway acts on several downstream effectors including phospholipase C gamma (PLCγ), cellular Src kinase (c-Src), phosphotidylinsitol-3-OH kinase (PI3K), alpha serine/threonine-protein kinase (Akt), mitogen-activated protein kinase (MAPK), and wingless-related integration site (Wnt) pathways. c-MET also establishes a crosstalk pathway with epidermal growth factor receptor (EGFR) and contributes towards chemoresistance in HNSCC. In recent years, the signaling communications of c-MET/HGF in metabolic dysregulation, tumor-microenvironment and immune modulation in HNSCC have emerged. Several clinical trials have been established against c-MET/ hepatocyte growth factor (HGF) signaling network to bring up targeted and effective therapeutic strategies against HNSCC. In this review, we discuss the molecular mechanism(s) and current understanding of c-MET/HGF signaling and its effect on HNSCC. Graphical abstract

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1476-4598Test

الوصول الحر: https://doaj.org/article/43904d49951f47cdbc75b69d633aa9c0Test

المؤلفون: Keshav Goyal, Harsh Goel, Pritika Baranwal, Anisha Tewary, Aman Dixit, Avanish Kumar Pandey, Mercilena Benjamin, Pranay Tanwar, Abhijit Dey, Fahad Khan, Pratibha Pandey, Piyush Kumar Gupta, Dhruv Kumar, Shubhadeep Roychoudhury, Niraj Kumar Jha, Tarun Kumar Upadhyay, Kavindra Kumar Kesari

المصدر: Immuno, Vol 1, Iss 4, Pp 442-456 (2021)

مصطلحات موضوعية: SARS-CoV-2, DNA vaccine, mRNA vaccine, adenoviral vector-based vaccine, Medicine

الوصف: The SARS-CoV-2 infection spread rapidly throughout the world and appears to involve in both humoral and cell-mediated immunity. SARS-CoV-2 is attached to host cells via binding to the viral spike (S) proteins and its cellular receptors angiotensin-converting enzyme 2 (ACE2). Consequently, the S protein is primed with serine proteases TMPRSS2 and TMPRSS4, which facilitate the fusion of viral and cellular membranes result in the entry of viral RNA into the host cell. Vaccines are urgently required to combat the coronavirus disease 2019 (COVID-19) outbreak and aid in the recovery to pre-pandemic levels of normality. The long-term protective immunity is provided by the vaccine antigen (or pathogen)-specific immune effectors and the activation of immune memory cells that can be efficiently and rapidly reactivated upon pathogen exposure. Research efforts aimed towards the design and development of vaccines for SARS-CoV-2 are increasing. Numerous coronavirus disease 2019 (COVID-19) vaccines have passed late-stage clinical investigations with promising outcomes. This review focuses on the present state and future prospects of COVID-19 vaccines research and development, with a particular emphasis on immunological mechanisms of various COVID-19vaccines such as adenoviral vector-based vaccines, mRNA vaccines, and DNA vaccines that elicits immunological responses against SARS-CoV-2 infections in humans.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2673-5601/1/4/32Test; https://doaj.org/toc/2673-5601Test

الوصول الحر: https://doaj.org/article/ec378af01f35420ca1eafcf01e215f30Test

المؤلفون: Maryam Faiyaz, Mohd. Azhardin Ganayee, Salman Akhtar, Saravanan Krishnan, Bableen Flora, Deeksha Dogra, Niraj Kumar Jha, Dinesh Kumar Chellappan, Poonam Negi, Kamal Dua, Kavindra Kumar Kesari, Piyush Kumar Gupta

المصدر: Frontiers in Bioscience-Landmark, Vol 26, Iss 10, Pp 851-865 (2021)

مصطلحات موضوعية: dementia, alzheimer’s disease, nanoparticles, nanomaterials, therapeutics, Biochemistry, QD415-436, Biology (General), QH301-705.5

الوصف: Alzheimer’s, a progressive neurodegenerative disease affects brain and neurons through enormous reduction in nerve cell regenerative capacity. Dementia and impairment of cognitive functions are more prevalent in Alzheimer’s disease (AD) patients in both industrialized and non-industrialized countries. Various factors play significant role in molecular cascades that leads to neuronal inflammation, dementia and thereby AD progression. Current medications are symptomatic that alleviates pain while lack in absolute cure, urging researchers to explore targets and therapeutics. Interestingly, nanomedicines developed due to the onset of nanotechnology, are being extensively investigated for the treatment of AD. This review presents the advancement in nanotherapeutic strategies, involving the emergence of nanomaterials that offers advantage to pass through the blood-brain barrier and acts as a therapeutic modality against AD.

وصف الملف: electronic resource

العلاقة: https://www.imrpress.com/journal/FBL/26/10/10.52586/4992Test; https://doaj.org/toc/2768-6701Test

الوصول الحر: https://doaj.org/article/e23a35510cdf4bb49c90141ee2daa907Test

المؤلفون: Niraj Kumar Jha, Charu Sharma, Mohamed Fizur Nagoor Meeran, Saurabh Kumar Jha, Vivek Dhar Dwivedi, Piyush Kumar Gupta, Abhijit Dey, Kavindra Kumar Kesari, Shreesh Ojha

المصدر: Immuno, Vol 1, Iss 3, Pp 285-304 (2021)

مصطلحات موضوعية: cannabinoids, CB2 receptors, COVID-19, immunomodulators, inflammation, JWH133, Medicine

الوصف: The COVID-19 pandemic, caused by SARS-CoV-2, is a deadly disease affecting millions due to the non-availability of drugs and vaccines. The majority of COVID-19 drugs have been repurposed based on antiviral, immunomodulatory, and antibiotic potential. The pathogenesis and advanced complications with infection involve the immune-inflammatory cascade. Therefore, a therapeutic strategy could reduce infectivity, inflammation, and immune modulation. In recent years, modulating the endocannabinoid system, particularly activation of the cannabinoid type 2 (CB2) receptor is a promising therapeutic target for modulation of immune-inflammatory responses. JWH133, a selective, full functional agonist of the CB2 receptor, has been extensively studied for its potent anti-inflammatory, antiviral, and immunomodulatory properties. JWH133 modulates numerous signaling pathways and inhibits inflammatory mediators, including cytokines, chemokines, adhesion molecules, prostanoids, and eicosanoids. In this study, we propose that JWH133 could be a promising candidate for targeting infection, immunity, and inflammation in COVID-19, due to its pharmacological and molecular mechanisms in numerous preclinical efficacy and safety studies, along with its immunomodulatory, anti-inflammatory, organoprotective, and antiviral properties. Thus, JWH133 should be investigated in preclinical and clinical studies for its potential as an agent or adjuvant with other agents for its effect on viremia, infectivity, immune modulation, resolution of inflammation, reduction in severity, and progression of complications in COVID-19. JWH133 is devoid of psychotropic effects due to CB2 receptor selectivity, has negligible toxicity, good bioavailability and druggable properties, including pharmacokinetic and physicochemical effects. We believe that JWH133 could be a promising drug and may inspire further studies for an evidence-based approach against COVID-19.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2673-5601/1/3/20Test; https://doaj.org/toc/2673-5601Test

الوصول الحر: https://doaj.org/article/d0dd19143da84d7cb62d904660c57dbdTest

المؤلفون: Sabya Sachi Das, Sandeep Kumar Singh, P.R.P. Verma, Rekha Gahtori, Belay Zeleke Sibuh, Kavindra Kumar Kesari, Niraj Kumar Jha, Sugapriya Dhanasekaran, Vijay Kumar Thakur, Ling Shing Wong, Sinouvassane Djearamane, Piyush Kumar Gupta

المصدر: Biomedicine & Pharmacotherapy, Vol 154, Iss , Pp 113654- (2022)

مصطلحات موضوعية: Inflammatory signaling pathways, Nanomedicine, Targeted delivery, Polyester nanomaterials, Anticancer, Therapeutics. Pharmacology, RM1-950

الوصف: The growth of cancerous cells and their responses towards substantial therapeutics are primarily controlled by inflammations (acute and chronic) and inflammation-associated products, which either endorse or repress tumor progression. Additionally, major signaling pathways, including NF-κB, STAT3, inflammation-causing factors (cytokines, TNF-α, chemokines), and growth-regulating factors (VEGF, TGF-β), are vital regulators responsible for the instigation and resolution of inflammations. Moreover, the conventional chemotherapeutics have exhibited diverse limitations, including poor pharmacokinetics, unfavorable chemical properties, poor targetability to the disease-specific disease leading to toxicity; thus, their applications are restricted in inflammation-mediated cancer therapy. Furthermore, nanotechnology has demonstrated potential benefits over conventional chemotherapeutics, such as it protected the incorporated drug/bioactive moiety from enzymatic degradation within the systemic circulation, improving the physicochemical properties of poorly aqueous soluble chemotherapeutic agents, and enhancing their targetability in specified carcinogenic cells rather than accumulating in the healthy cells, leading reduced cytotoxicity. Among diverse nanomaterials, polyester-based nanoparticulate delivery systems have been extensively used to target various inflammation-mediated cancers. This review summarizes the therapeutic potentials of various polyester nanomaterials (PLGA, PCL, PLA, PHA, and others)-based delivery systems targeting multiple signaling pathways related to inflammation-mediated cancer.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S0753332222010435Test; https://doaj.org/toc/0753-3322Test

الوصول الحر: https://doaj.org/article/0ec0811a1b5f4b86a9cbe955e49d91b6Test

المؤلفون: Sinouvassane Djearamane, Zhe Chi Loh, Jun Jie Lee, Ling Shing Wong, Ranjithkumar Rajamani, Priscy Alfredo Luque, Piyush Kumar Gupta, Sharolynne Xiao Tong Liang

المصدر: Frontiers in Pharmacology, Vol 13 (2022)

مصطلحات موضوعية: zinc oxide nanoparticles, antibacterial agent, S. marcescens, E. faecalis, growth inhibition, Therapeutics. Pharmacology, RM1-950

الوصف: Zinc oxide nanoparticles (ZnO NPs) have been widely used in biomedical applications due to their high biocompatibility and low toxicity to humans. The present work aimed to investigate the antibacterial effects of different concentrations of ZnO NPs on two opportunistic pathogens, Serratia marcescens and Enterococcus faecalis. The surface interaction between nanoparticles and bacterial cell wall, and the subsequent morphological alterations on the bacterial surface, were examined through Fourier transform infrared spectroscopy and scanning electron microscope. The energy dispersive X-ray analysis was used to confirm the elemental composition of ZnO NPs and the cellular accumulation of ZnO NPs in bacteria. The growth-inhibitory test demonstrated a dose-dependent growth inhibitory effect of ZnO NPs against both the test bacteria, as the higher concentration of nanoparticles caused the higher bacterial growth inhibition. The results showed that ZnO NPs caused a higher growth inhibition (63.50 ± 2.50%) on the Gram-positive bacterium E. faecalis compared to the Gram-negative bacterium S. marcescens (51.27 ± 4.56%). Fourier transform infrared spectrum revealed the possible involvement of hydroxyl, carboxyl, amides, methylene, and phosphate groups from the biomolecules of bacterial cell wall such as proteins, carbohydrates, lipids, and phospholipids in the interaction of ZnO NPs on bacterial cell surface. Energy dispersive X-ray analysis showed the higher accumulation of ZnO NPs in E. faecalis than S. marcescens analogous to the bacterial growth inhibition. Scanning electron microscopy images confirmed the antibacterial properties of ZnO NPs, showing the loss of integrity of cell membrane and distortion of bacterial cells. Hence, the potential of ZnO NP as an antibacterial agent against S. marcescens and E. faecalis has been confirmed.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fphar.2022.891304/fullTest; https://doaj.org/toc/1663-9812Test

الوصول الحر: https://doaj.org/article/eff150a6af11463f8ed412dccd0fea52Test

المؤلفون: Tenzin Pema, Ankit Kumar, Babita Tripathi, Soumya Pandit, Sunil Chauhan, Satyendra Singh, Pritam Kumar Dikshit, Abhilasha Singh Mathuriya, Piyush Kumar Gupta, Dibyajit Lahiri, Ram Chandra Singh, Jigisha Anand, Kundan Kumar Chaubey

المصدر: Catalysts, Vol 13, Iss 3, p 618 (2023)

مصطلحات موضوعية: bismuth ferrite, graphene, microbial fuel cell, oxidation-reduction reaction, cathode, power density, Chemical technology, TP1-1185, Chemistry, QD1-999

الوصف: In this study, multifunctional lithium-doped bismuth ferrite [BiFe1−xLixO3]-graphene nanocomposites (x = 0.00, 0.02, 0.04, 0.06) were synthesized by a sol-gel and ultrasonication assisted chemical reduction method. X-ray diffraction and FESEM electron microscopy techniques disclosed the nanocomposite phase and nanocrystalline nature of [BiFe1−xLixO3]-graphene nanocomposites. The FESEM images and the EDX elemental mapping revealed the characteristic integration of BiFe1−xLixO3 nanoparticles (with an average size of 95 nm) onto the 2D graphene layers. The Raman spectra of the [BiFe1−xLixO3]-graphene nanocomposites evidenced the BiFe1−xLixO3 and graphene nanostructures in the synthesized nanocomposites. The photocatalytic performances of the synthesized nanocomposites were assessed for ciprofloxacin (CIP) photooxidation under UV-visible light illumination. The photocatalytic efficiencies of [BiFe1−xLixO3]-graphene nanocomposites were measured to be 42%, 47%, 43%, and 10%, for x = 0.00, 0.02, 0.04, 0.06, respectively, within 120 min illumination, whereas the pure BiFeO3 nanoparticles were 21.0%. BiFe1−xLixO3 nanoparticles blended with graphene were explored as cathode material and tested in a microbial fuel cell (MFC). The linear sweep voltammetry (LSV) analysis showed that the high surface area of BiFeO3 was attributed to efficient oxygen reduction reaction (ORR) activity. The increasing loading rates of (0.5–2.5 mg/cm2) [BiFe1−xLixO3]-graphene composite on the cathode surface showed increasing power output, with 2.5 and 2 mg/cm2 achieving the maximum volumetric power density of 8.2 W/m3 and 8.1 W/m3, respectively. The electrochemical impedance spectroscopy (EIS) analysis showed that among the different loading rates used in this study, BiFeO3, with a loading rate of 2.5 mg/cm2, showed the lowest charge transfer resistance (Rct). The study results showed the potential of [BiFe1−xLixO3]-graphene composite as a cost-effective alternative for field-scale MFC applications.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2073-4344/13/3/618Test; https://doaj.org/toc/2073-4344Test

الوصول الحر: https://doaj.org/article/598851a6a9654bf9b23f97f8e3b360f6Test