يعرض 1 - 10 نتائج من 1,660 نتيجة بحث عن '"Pietrzyk, Jacek A."', وقت الاستعلام: 1.15s تنقيح النتائج
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    دورية أكاديمية

    الوصف: Background: We aimed to identify global blood and retinal gene expression patterns in murine oxygen-induced retinopathy (OIR), a common model of retinopathy of prematurity, which may allow better understanding of the pathogenesis of this severe ocular prematurity complication and identification of potential blood biomarkers. Methods: A total of 120 C57BL/6J mice were randomly divided into an OIR group, in which 7-day-old pups were maintained in 75% oxygen for 5 days, or a control group. RNA was extracted from the whole-blood mononuclear cells and retinal cells on days 12, 17, and 28. Gene expression in the RNA samples was evaluated with mouse gene expression microarrays. Results: There were 38, 1370 and 111 genes, the expression of which differed between the OIR and control retinas on days 12, 17, and 28, respectively. Gene expression in the blood mononuclear cells was significantly altered only on day 17. Deptor and Nol4 genes showed reduced expression both in the blood and retinal cells on day 17. Conclusion There are sustained marked changes in the global pattern of gene expression in the OIR mice retinas. An altered expression of Deptor and Nol4 genes in the blood mononuclear cells requires further investigation as they may indicate retinal neovascularization.

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    كتاب

    المساهمون: Uliasz-Bocheńczyk, Alicja., Kępys, Waldemar., Akademia Górniczo-Hutnicza im. Stanisława Staszica (Kraków). Katedra Inżynierii Środowiska i Przeróbki Surowców. Instytucja nadająca stopień

    المصدر: Bibliografia na kartach 125-145.

    Degree: Praca doktorska. Akademia Górniczo-Hutnicza im. Stanisława Staszica. Wydział Górnictwa i Geoinżynierii. Katedra Inżynierii Środowiska i Przeróbki Surowców, 2019.

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    دورية أكاديمية

    المصدر: 0031-3998.

    الوصف: Background We aimed to identify global blood and retinal gene expression patterns in murine oxygen-induced retinopathy (OIR), a common model of retinopathy of prematurity, which may allow better understanding of the pathogenesis of this severe ocular prematurity complication and identification of potential blood biomarkers. Methods A total of 120 C57BL/6J mice were randomly divided into an OIR group, in which 7-day-old pups were maintained in 75% oxygen for 5 days, or a control group. RNA was extracted from the whole-blood mononuclear cells and retinal cells on days 12, 17, and 28. Gene expression in the RNA samples was evaluated with mouse gene expression microarrays. Results There were 38, 1370 and 111 genes, the expression of which differed between the OIR and control retinas on days 12, 17, and 28, respectively. Gene expression in the blood mononuclear cells was significantly altered only on day 17. Deptor and Nol4 genes showed reduced expression both in the blood and retinal cells on day 17. Conclusion There are sustained marked changes in the global pattern of gene expression in the OIR mice retinas. An altered expression of Deptor and Nol4 genes in the blood mononuclear cells requires further investigation as they may indicate retinal neovascularization.

    العلاقة: http://urn.nb.no/URN:NBN:no-80346Test; Zasada, Magdalena Madetko-Talowska, Anna Revhaug, Cecilie Rognlien, Anne Gro Wesenberg Baumbusch, Lars Oliver Książek, Teofila Szewczyk, Katarzyna Grabowska, Agnieszka Bik-Multanowski, Miroslaw Józef Pietrzyk, Jacek Kwinta, Przemko Saugstad, Ola Didrik . Short- and long-term impact of hyperoxia on the blood and retinal cells' transcriptome in a mouse model of oxygen-induced retinopathy. Pediatric Research. 2019, 1-9; http://hdl.handle.net/10852/77239Test; 1751861; info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Pediatric Research&rft.volume=&rft.spage=1&rft.date=2019; Pediatric Research; 87; 485; 493; https://doi.org/10.1038/s41390-019-0598-yTest; URN:NBN:no-80346; Fulltext https://www.duo.uio.no/bitstream/handle/10852/77239/2/Zasada_et_al_Ped_research_2019.pdfTest

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    دورية أكاديمية

    الوصف: Background In this study, we aimed to analyze differences in plasma protein abundances between infants with and without bronchopulmonary dysplasia (BPD), to add new insights into a better understanding of the pathogenesis of this disease. Methods Cord and peripheral blood of neonates (≤ 30 weeks gestational age) was drawn at birth and at the 36th postmenstrual week (36 PMA), respectively. Blood samples were retrospectively subdivided into BPD(+) and BPD(−) groups, according to the development of BPD. Results Children with BPD were characterized by decreased afamin, gelsolin and carboxypeptidase N subunit 2 levels in cord blood, and decreased galectin-3 binding protein and hemoglobin subunit gamma-1 levels, as well as an increased serotransferrin abundance in plasma at the 36 PMA. Conclusions BPD development is associated with the plasma proteome changes in preterm infants, adding further evidence for the possible involvement of disturbances in vitamin E availability and impaired immunological processes in the progression of prematurity pulmonary complications. Moreover, it also points to the differences in proteins related to infection resistance and maintaining an adequate level of hematocrit in infants diagnosed with BPD.

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    دورية أكاديمية

    المصدر: 0146-0404.

    الوصف: Purpose: Retinopathy of prematurity (ROP) is a vision-threatening complication of a premature birth, in which the etiology still remains unclear. Importantly, the molecular processes that govern these effects can be investigated in a perturbed plasma proteome composition. Thus, plasma proteomics may add new insights into a better understanding of the pathogenesis of this disease. Methods: The cord and peripheral blood of neonates (≤30 weeks gestational age) was drawn at birth and at the 36th postmenstrual week (PMA), respectively. Blood samples were retrospectively subdivided into ROP(+) and ROP(−) groups, according to the development of ROP. Results: The quantitative analysis of plasma proteome at both time points revealed 30 protein abundance changes between ROP(+) and ROP(−) groups. After standardization to gestational age, children who developed ROP were characterized by an increased C3 complement component and fibrinogen level at both analyzed time points. Conclusions: Higher levels of the complement C3 component and fibrinogen, present in the cord blood and persistent to 36 PMA, may indicate a chronic low-grade systemic inflammation and hypercoagulable state that may play a role in the development of ROP.

    العلاقة: http://urn.nb.no/URN:NBN:no-70742Test; Zasada, Magdalena Suski, Maciej Bokiniec, Renata Szwarc-Duma, Monika Borszewska-Kornacka, Maria Katarzyna Madej, Józef Bujak-Giżycka, Beata Madetko-Talowska, Anna Revhaug, Cecilie Baumbusch, Lars Oliver Saugstad, Ola Didrik Pietrzyk, Jacek Józef Kwinta, Przemko . An iTRAQ-based quantitative proteomic analysis of plasma proteins in preterm newborns with retinopathy of prematurity. Investigative Ophthalmology and Visual Science. 2018, 59(13), 5312-5319; http://hdl.handle.net/10852/67586Test; 1648087; info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Investigative Ophthalmology and Visual Science&rft.volume=59&rft.spage=5312&rft.date=2018; Investigative Ophthalmology and Visual Science; 59; 13; 5312; 5319; http://dx.doi.org/10.1167/iovs.18-24914Test; URN:NBN:no-70742; Fulltext https://www.duo.uio.no/bitstream/handle/10852/67586/2/An%2BiTRAQ-Based%2BQuantitative%2BProteomic%2BAnalysis%2Bof%2BPlasma%2BProteins%2Bin%2BPreterm%2BNewborns%2BWith%2BRetinopathy%2Bof%2BPrematurity.pdfTest

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    كتاب
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    دورية أكاديمية
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    دورية أكاديمية