المؤلفون: Raphaël Coquerel, Mustapha Arkoun, Quentin Dupas, Fanny Leroy, Philippe Laîné, Philippe Etienne

المصدر: Plants, Vol 12, Iss 12, p 2248 (2023)

مصطلحات موضوعية: N2 fixation, nodules, S deficiency, silicon, 15N labeling, Trifolium incarnatum, Botany, QK1-989

الوصف: In many crops species, sulfur (S) deprivation negatively affects growth, seed yield quality and plant health. Furthermore, silicon (Si) is known to alleviate many nutritional stresses but the effects of Si supply on plants subjected to S deficiency remain unclear and poorly documented. The objective of this study was to evaluate whether Si supply would alleviate the negative effects of S deprivation on root nodulation and atmospheric dinitrogen (N2) fixation capacity in Trifolium incarnatum subjected (or not) to long-term S deficiency. For this, plants were grown for 63 days in hydroponic conditions with (500 µM) or without S and supplied (1.7 mM) or not with Si. The effects of Si on growth, root nodulation and N2 fixation and nitrogenase abundance in nodules have been measured. The most important beneficial effect of Si was observed after 63 days. Indeed, at this harvest time, a Si supply increased growth, the nitrogenase abundance in nodules and N2 fixation in S-fed and S-deprived plants while a beneficial effect on the number and total biomass of nodules was only observed in S-deprived plants. This study shows clearly for the first time that a Si supply alleviates negative effects of S deprivation in Trifolium incarnatum.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2223-7747/12/12/2248Test; https://doaj.org/toc/2223-7747Test

الوصول الحر: https://doaj.org/article/1d949f2f142d4ce1a1b74c330f5a74e0Test

المؤلفون: Aurélien D'Oria, Galatéa Courbet, Bastien Billiot, Lun Jing, Sylvain Pluchon, Mustapha Arkoun, Anne Maillard, Christine Paysant‐Le Roux, Jacques Trouverie, Philippe Etienne, Sylvain Diquélou, Alain Ourry

المصدر: Plant Direct, Vol 6, Iss 8, Pp n/a-n/a (2022)

مصطلحات موضوعية: ionome, iron, known ionomic genes, manganese, molybdenum, nutrient, Botany, QK1-989

الوصف: Abstract One of the main limiting factors of plant yield is drought, and while the physiological responses to this environmental stress have been broadly described, research addressing its impact on mineral nutrition is scarce. Brassica napus and Triticum aestivum were subjected to moderate or severe water deficit, and their responses to drought were assessed by functional ionomic analysis, and derived calculation of the net uptake of 20 nutrients. While the uptake of most mineral nutrients decreased, Fe, Zn, Mn, and Mo uptake were impacted earlier and at a larger scale than most physiological parameters assessed (growth, ABA concentration, gas exchanges and photosynthetic activity). Additionally, in B. napus, the patterns of 183 differentially expressed genes in leaves related to the ionome (known ionomic genes, KIGs) or assumed to be involved in transport of a given nutrient were analyzed. This revealed three patterns of gene expression under drought consisting of up (transport of Cl and Co), down (transport of N, P, B, Mo, and Ni), or mixed levels (transport of S, Mg, K, Zn, Fe, Cu, or Mn) of regulation. The three patterns of gene regulations are discussed in relation to specific gene functions, changes of leaf ionomic composition and with consideration of the crosstalks that have been established between elements. It is suggested that the observed reduction in Fe uptake occurred via a specific response to drought, leading indirectly to reduced uptake of Zn and Mn, and these may be taken up by common transporters encoded by genes that were downregulated.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2475-4455Test

الوصول الحر: https://doaj.org/article/1e07360af3bf46d4828990f409791b4cTest

المؤلفون: Philippe Laîné, Raphaël Coquerel, Mustapha Arkoun, Jacques Trouverie, Philippe Etienne

المصدر: Plants, Vol 11, Iss 12, p 1606 (2022)

مصطلحات موضوعية: root sulfate transporters, S deficiency, silicon, sulfate influx, 34SO42−, transcriptional regulation, Botany, QK1-989

الوصف: Silicon (Si) is known to alleviate many nutritional stresses. However, in Brassica napus, which is a highly S-demanding species, the Si effect on S deficiency remains undocumented. The aim of this study was to assess whether Si alleviates the negative effects of S deficiency on Brassica napus and modulates root sulfate uptake capacity and S accumulation. For this, Brassica napus plants were cultivated with or without S and supplied or not supplied with Si. The effects of Si on S content, growth, expression of sulfate transporter genes (BnaSultr1.1; BnaSultr1.2) and sulfate transporters activity in roots were monitored. Si supply did not mitigate growth or S status alterations due to S deprivation but moderated the expression of BnaSultr1.1 in S-deprived plants without affecting the activity of root sulfate transporters. The effects of Si on the amount of S taken-up and on S transporter gene expression were also evaluated after 72 h of S resupply. In S-deprived plants, S re-feeding led to a strong decrease in the expression of both S transporter genes as expected, except in Si-treated plants where BnaSultr1.1 expression was maintained over time. This result is discussed in relation to the similar amount of S accumulated regardless of the Si treatment.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2223-7747/11/12/1606Test; https://doaj.org/toc/2223-7747Test

الوصول الحر: https://doaj.org/article/f40e81fe1d3c4d408d06b26150e4e8a8Test

المؤلفون: Galatéa Courbet, Aurélien D’Oria, Aurélia Lornac, Sylvain Diquélou, Sylvain Pluchon, Mustapha Arkoun, Anna Koprivova, Stanislav Kopriva, Philippe Etienne, Alain Ourry

المصدر: Frontiers in Plant Science, Vol 12 (2021)

مصطلحات موضوعية: ionome, nutrient deficiencies, nutrient interactions, oilseed rape, sodium, vanadium, Plant culture, SB1-1110

الوصف: The composition of the functional ionome was studied in Brassica napus and Triticum aestivum with respect to the response of 20 elements under macronutrient deprivation. Analysis of relative root contents showed that some nutrients, such as Fe, Ni, Cu, Na, V, and Co, were largely sequestered in roots. After 10 days of deprivation of each one of these 6 macronutrients, plant growth was similar to control plants, and this was probably the result of remobilization from roots (Mg and Ca) or old leaves (N, P, K, S). Some tissue concentrations and net nutrient uptakes into roots were either decreased or increased, revealing multiple interactions (93 in wheat, 66 in oilseed rape) that were common to both species (48) or were species specific. While some interactions have been previously described (increased uptake of Na under K deficiency; or increased uptake of Mo and Se under S deficiency), a number of new interactions were found and some key mechanisms underlying their action have been proposed from analysis of Arabidopsis mutants. For example, nitrate uptake seemed to be functionally linked to Na(influx, while the uptake of vanadium was probably mediated by sulfate transporters whose expression was stimulated during S deprivation.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fpls.2021.641648/fullTest; https://doaj.org/toc/1664-462XTest

الوصول الحر: https://doaj.org/article/ed49eaaff2c147cc94f5f5c9e9046691Test

المؤلفون: Aurélien D’Oria, Galatéa Courbet, Aurélia Lornac, Sylvain Pluchon, Mustapha Arkoun, Anne Maillard, Philippe Etienne, Sylvain Diquélou, Alain Ourry

المصدر: Frontiers in Plant Science, Vol 12 (2021)

مصطلحات موضوعية: ionome, ionomic signatures, nutrient deficiency, nutrient interactions, rapeseed, wheat, Plant culture, SB1-1110

الوصف: The specific variation in the functional ionome was studied in Brassica napus and Triticum aestivum plants subjected to micronutrient or beneficial mineral nutrient deprivation. Effects of these deprivations were compared to those of macronutrient deprivation. In order to identify early events, plants were harvested after 22 days, i.e., before any significant reduction in growth relative to control plants. Root uptake, tissue concentrations and relative root nutrient contents were analyzed revealing numerous interactions with respect to the 20 elements quantified. The assessment of the functional ionome under individual mineral nutrient deficiency allows the identification of a large number of interactions between elements, although it is not totally exhaustive, and gives access to specific ionomic signatures that discriminate among deficiencies in N, P, S, K, Ca, Mn, Fe, Zn, Na, Si, and Se in both species, plus Mg, Cl, Cu, and Mo in wheat. Ionome modifications and components of ionomic signatures are discussed in relation to well-known mechanisms that may explain crosstalks between mineral nutrients, such as between Na and K, V, Se, Mo and S or Fe, Zn and Cu. More surprisingly, when deprived of beneficial nutrients such as Na, Si, Co, or Se, the plant ionome was strongly modified while these beneficial nutrients contributed greatly to the leaf ionomic signature of most mineral deficiencies.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fpls.2021.641678/fullTest; https://doaj.org/toc/1664-462XTest

الوصول الحر: https://doaj.org/article/9532555819a7432dad29c89984dd0699Test

المؤلفون: Aurélien D’Oria, Lun Jing, Mustapha Arkoun, Sylvain Pluchon, Stéphanie Pateyron, Jacques Trouverie, Philippe Etienne, Sylvain Diquélou, Alain Ourry

المصدر: International Journal of Molecular Sciences, Vol 23, Iss 2, p 781 (2022)

مصطلحات موضوعية: abscisic acid, genes related to transport, glutathione, ionome, jasmonic acid, mineral nutrition, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: While it is generally acknowledged that drought is one of the main abiotic factors affecting plant growth, how mineral nutrition is specifically and negatively affected by water deficit has received very little attention, other than being analyzed as a consequence of reduced growth. Therefore, Brassica napus plants were subjected to a gradual onset of water deficits (mild, severe, or severe extended), and leaves were analyzed at the ionomic, transcriptomic and metabolic levels. The number of Differentially Expressed Genes (DEGs) and of the most differentially accumulated metabolites increased from mild (525 DEGs, 57 metabolites) to severe (5454 DEGs, 78 metabolites) and severe extended (9346 DEGs, 95 metabolites) water deficit. Gene ontology enrichment analysis of the 11,747 DEGs identified revealed that ion transport was one of the most significant processes affected, even under mild water deficit, and this was also confirmed by the shift in ionomic composition (mostly micronutrients with a strong decrease in Mo, Fe, Zn, and Mn in leaves) that occurred well before growth reduction. The metabolomic data and most of the transcriptomic data suggested that well-known early leaf responses to drought such as phytohormone metabolism (ABA and JA), proline accumulation, and oxidative stress defense were induced later than repression of genes related to nutrient transport.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1422-0067/23/2/781Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test

الوصول الحر: https://doaj.org/article/724fd9cb09ca4544addc71847c10dc02Test

المؤلفون: Galatéa Courbet, Aurélien D’Oria, Anne Maillard, Lun Jing, Sylvain Pluchon, Mustapha Arkoun, Stéphanie Pateyron, Christine Paysant Le Roux, Sylvain Diquélou, Alain Ourry, Jacques Trouverie, Philippe Etienne

المصدر: International Journal of Molecular Sciences, Vol 22, Iss 21, p 11679 (2021)

مصطلحات موضوعية: macronutrient deprivations, metabolic pathways, metabolomic, molecular indicators, regulations of nutrient metabolism, transcriptomic, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: The early and specific diagnosis of a macronutrient deficiency is challenging when seeking to better manage fertilizer inputs in the context of sustainable agriculture. Consequently, this study explored the potential for transcriptomic and metabolomic analysis of Brassica napus roots to characterize the effects of six individual macronutrient deprivations (N, Mg, P, S, K, and Ca). Our results showed that before any visual phenotypic response, all macronutrient deprivations led to a large modulation of the transcriptome and metabolome involved in various metabolic pathways, and some were common to all macronutrient deprivations. Significantly, comparative transcriptomic analysis allowed the definition of a subset of 3282, 2011, 6325, 1384, 439, and 5157 differentially expressed genes (DEGs) specific to N, Mg, P, S, K, and Ca deprivations, respectively. Surprisingly, gene ontology term enrichment analysis performed on this subset of specific DEGs highlighted biological processes that are common to a number of these macronutrient deprivations, illustrating the complexity of nutrient interactions. In addition, a set of 38 biochemical compounds that discriminated the macronutrient deprivations was identified using a metabolic approach. The opportunity to use these specific DEGs and/or biochemical compounds as potential molecular indicators to diagnose macronutrient deficiency is discussed.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1422-0067/22/21/11679Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test

الوصول الحر: https://doaj.org/article/36a86bcfca73411a8d90170009995fa4Test

المؤلفون: Maxence James, Céline Masclaux-Daubresse, Anne Marmagne, Marianne Azzopardi, Philippe Laîné, Didier Goux, Philippe Etienne, Jacques Trouverie

المصدر: Frontiers in Plant Science, Vol 9 (2019)

مصطلحات موضوعية: cysteine protease activity, N remobilization, reproductive stage, roots, SAG12, N uptake, Plant culture, SB1-1110

الوصف: Senescence associated gene (SAG) 12, which encodes a cysteine protease is considered to be important in nitrogen (N) allocation to Arabidopsis thaliana seeds. A decrease in the yield and N content of the seeds was observed in the Arabidopsis SAG12 knockout mutants (sag12) relative to the wild type (Col0) under limited nitrogen nutrition. However, leaf senescence was similar in both lines. To test whether SAG12 is involved in N remobilization from organs other than the leaves, we tested whether root N could be used in N mobilization to the seeds. Root architecture, N uptake capacity and 15N partitioning were compared in the wild type and sag12 under either high nitrogen (HN) or low nitrogen (LN) conditions. No differences in root architecture or root N uptake capacity were observed between the lines under HN or LN. However, under LN conditions, there was an accumulation of 15N in the sag12 roots compared to the wild type with lower allocation of 15N to the seeds. This was accompanied by an increase in root N protein contents and a significant decrease in root cysteine protease activity. SAG12 is expressed in the root stele of the plants at the reproductive stage, particularly under conditions of LN nutrition. Taken together, these results suggest a new role for SAG12. This cysteine protease plays a crucial role in root N remobilization that ensures seed filling and sustains yields when nitrogen availability is low.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/article/10.3389/fpls.2018.01998/fullTest; https://doaj.org/toc/1664-462XTest

الوصول الحر: https://doaj.org/article/0e7569695bb748aaae244b9b9876c480Test

المؤلفون: Cylia Haddad, Mustapha Arkoun, Franck Jamois, Adrian Schwarzenberg, Jean-Claude Yvin, Philippe Etienne, Philippe Laîné

المصدر: Frontiers in Plant Science, Vol 9 (2018)

مصطلحات موضوعية: N privation, N uptake, SAG12/Cab indicator, leaf life span, photosynthetic activity, Plant culture, SB1-1110

الوصف: Silicon (Si) is the second most abundant element in soil and has several beneficial effects, especially in plants subjected to stress conditions. However, the effect of Si in preventing nitrogen (N) starvation in plants is poorly documented. The aim of this work was to study the effect of a short Si supply duration (7 days) on growth, N uptake, photosynthetic activity, and leaf senescence progression in rapeseed subjected (or not) to N starvation. Our results showed that after 1 week of Si supply, Si improves biomass and increases N uptake and root expression of a nitrate transporter gene. After 12 days of N starvation, compared to -Si plants, mature leaf from +Si plants showed a high chlorophyll content, a maintain of net photosynthetic activity, a decrease of oxidative stress markers [hydrogen peroxide (H2O2) and malondialdehyde (MDA)] and a significant delay in senescence. When N-deprived plants were resupplied with N, a greening again associated with an increase of photosynthetic activity was observed in mature leaves of plants pretreated with Si. Moreover, during the duration of N resupply, an increase of N uptake and nitrate transporter gene expression were observed in plants pretreated with Si. In conclusion, this study has shown a beneficial role of Si to alleviate damage associated with N starvation and more especially its role in delaying of leaf senescence.

وصف الملف: electronic resource

العلاقة: http://journal.frontiersin.org/article/10.3389/fpls.2018.00516/fullTest; https://doaj.org/toc/1664-462XTest

الوصول الحر: https://doaj.org/article/1f90aac5919b44a88fc9675ebb9d1c47Test

المؤلفون: Thaïs Génard, Philippe Etienne, Sylvain Diquélou, Jean-Claude Yvin, Cécile Revellin, Philippe Laîné

المصدر: Heliyon, Vol 3, Iss 3 (2017)

مصطلحات موضوعية: Agriculture, Plant biology, Science (General), Q1-390, Social sciences (General), H1-99

الوصف: In this study we tested whether legumes can improve the growth and N and S nutrition of rapeseed in an intercropping system and compared the effect of mixtures on legume N-fixation and soil N-resources. Rapeseed was cultivated in low N conditions in monocrops using one (R) or two plants (RR) per pot and in mixtures with lupine, clover or vetch. The R monocrop was the most relevant control, intraspecific competition inducing a significant growth delay resulting in a significantly lower leaf number, in RR monocrop compared to R and the three mixtures considered. Plant biomass, and the N and S contents of rapeseed grown in mixtures were the same than those measured in R monocrop. Compared to the monocrop, the proportion of N derived from the atmosphere was increased by 34, 140 and 290% in lupine, clover and vetch, respectively when intercropped with rapeseed. In mixture with clover and lupine, the soil N pool at harvest was higher than in other treatments, while N export by crop was constant. Legumes suffered from competition for soil S resulting in a decrease of 40% in their S content compared to the monocrop. Compared to rapeseeds grown in R monocrop and in mixture with lupine and vetch, rapeseed mixed with clover showed significantly higher SPAD values in old leaves. In our conditions, mixing legumes with rapeseed is relevant to reduce N fertilization and improve nutrition and growth of rapeseed.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405844016321582Test; https://doaj.org/toc/2405-8440Test

الوصول الحر: https://doaj.org/article/f0631296f3f945df90be0ecf2f3a6927Test