المؤلفون: Gabaldón, Tony, OPATHY Consortium, the, Arastehfar, A, Boekhout, T, Butler, G, Buda De Cesare, G, Dolk, E, Hafez, A, Hube, B, Hagen, F, Hovhannisyan, H, Iracane, E, Kostrzewa, M, Lackner, M, Lass-Flörl, C, Llorens, C, Mixão, V, Munro, C, Oliveira-Pacheco, J, Pekmezovic, M, Pérez-Hansen, A, Rodriguez Sánchez, Antonio, Sauer, FM, Sparbier, K, Stavrou, AA, Vaneechoutte, Mario, Vatanshenassan, M

المصدر: FEMS MICROBIOLOGY REVIEWS ; ISSN: 0168-6445 ; ISSN: 1574-6976

مصطلحات موضوعية: Medicine and Health Sciences, Biology and Life Sciences, yeast pathogens, diagnosis, Candida, candidemia, sequencing, proteomics, DESORPTION IONIZATION-TIME, FLIGHT MASS-SPECTROMETRY, IN-SITU HYBRIDIZATION, MALDI-TOF MS, CULTURE IDENTIFICATION PANEL, CLINICAL-PRACTICE GUIDELINE, ESCMID-ASTERISK GUIDELINE, BLOOD-STREAM INFECTIONS, LASER-DESORPTION/IONIZATION-TIME, INTERNAL TRANSCRIBED SPACER

الوصف: The incidence of opportunistic yeast infections in humans has been increasing over recent years. These infections are difficult to treat and diagnose, in part due to the large number and broad diversity of species that can underlie the infection. In addition, resistance to one or several antifungal drugs in infecting strains is increasingly being reported, severely limiting therapeutic options and showcasing the need for rapid detection of the infecting agent and its drug susceptibility profile. Current methods for species and resistance identification lack satisfactory sensitivity and specificity, and often require prior culturing of the infecting agent, which delays diagnosis. Recently developed high-throughput technologies such as next generation sequencing or proteomics are opening completely new avenues for more sensitive, accurate and fast diagnosis of yeast pathogens. These approaches are the focus of intensive research, but translation into the clinics requires overcoming important challenges. In this review, we provide an overview of existing and recently emerged approaches that can be used in the identification of yeast pathogens and their drug resistance profiles. Throughout the text we highlight the advantages and disadvantages of each methodology and discuss the most promising developments in their path from bench to bedside.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/8640794Test; http://hdl.handle.net/1854/LU-8640794Test; http://dx.doi.org/10.1093/femsre/fuz015Test; https://biblio.ugent.be/publication/8640794/file/8640795Test

الإتاحة: https://doi.org/10.1093/femsre/fuz015Test
https://biblio.ugent.be/publication/8640794Test
http://hdl.handle.net/1854/LU-8640794Test
https://biblio.ugent.be/publication/8640794/file/8640795Test

المؤلفون: Arastehfar, A, Boekhout, T, Butler, G, De Cesare, G Buda, Dolk, E, Gabaldón, T, Hafez, A, Hube, B, Hagen, F, Hovhannisyan, H, Iracane, E, Kostrzewa, M, Lackner, M, Lass-Flörl, C, Llorens, C, Mixão, V, Munro, C, Oliveira-Pacheco, J, Pekmezovic, M, Pérez-Hansen, A, Sanchez, A Rodriguez, Sauer, F M, Sparbier, K, Stavrou, A A, Vaneechoutte, M, Vatanshenassan, M, Gabaldón, Toni

المساهمون: European Union’s Horizon 2020 research, Marie Sklodowska-Curie

المصدر: FEMS Microbiology Reviews ; volume 43, issue 5, page 517-547 ; ISSN 1574-6976

الوصف: The incidence of opportunistic yeast infections in humans has been increasing over recent years. These infections are difficult to treat and diagnose, in part due to the large number and broad diversity of species that can underlie the infection. In addition, resistance to one or several antifungal drugs in infecting strains is increasingly being reported, severely limiting therapeutic options and showcasing the need for rapid detection of the infecting agent and its drug susceptibility profile. Current methods for species and resistance identification lack satisfactory sensitivity and specificity, and often require prior culturing of the infecting agent, which delays diagnosis. Recently developed high-throughput technologies such as next generation sequencing or proteomics are opening completely new avenues for more sensitive, accurate and fast diagnosis of yeast pathogens. These approaches are the focus of intensive research, but translation into the clinics requires overcoming important challenges. In this review, we provide an overview of existing and recently emerged approaches that can be used in the identification of yeast pathogens and their drug resistance profiles. Throughout the text we highlight the advantages and disadvantages of each methodology and discuss the most promising developments in their path from bench to bedside.

الإتاحة: https://doi.org/10.1093/femsre/fuz015Test
http://academic.oup.com/femsre/article-pdf/43/5/517/37020972/fuz015.pdfTest

المؤلفون: Hagen F, Lumbsch HT, Arsic Arsenijevic V, Badali H, Bertout S, Billmyre RB, Bragulat MR, Cabañes FJ, Carbia M, Chakrabarti A, Chaturvedi S, Chaturvedi V, Chen M, Chowdhary A, Colom MF, Cornely OA, Crous PW, Cuétara MS, Diaz MR, Espinel Ingroff A, Fakhim H, Falk R, Fang W, Herkert PF, Ferrer Rodríguez C, Fraser JA, Gené J, Guarro J, Idnurm A, Illnait Zaragozi MT, Khan Z, Khayhan K, Kolecka A, Kurtzman CP, Lagrou K, Liao W, Linares C, Meis JF, Nielsen K, Nyazika TK, Pan W, Pekmezovic M, Polacheck I, Posteraro B, de Queiroz Telles F. Filho, ROMEO, ORAZIO, Sánchez M, Sampaio A, Sanguinetti M, Sriburee P, Sugita T, Taj Aldeen SJ, Takashima M, Taylor JW, Theelen B, Tomazin R, Verweij PE, Wahyuningsih R, Wang P, Boekhout T.

المساهمون: Hagen, F, Lumbsch, Ht, Arsic Arsenijevic, V, Badali, H, Bertout, S, Billmyre, Rb, Bragulat, Mr, Cabañes, Fj, Carbia, M, Chakrabarti, A, Chaturvedi, S, Chaturvedi, V, Chen, M, Chowdhary, A, Colom, Mf, Cornely, Oa, Crous, Pw, Cuétara, M, Diaz, Mr, Espinel Ingroff, A, Fakhim, H, Falk, R, Fang, W, Herkert, Pf, Ferrer Rodríguez, C, Fraser, Ja, Gené, J, Guarro, J, Idnurm, A, Illnait Zaragozi, Mt, Khan, Z, Khayhan, K, Kolecka, A, Kurtzman, Cp, Lagrou, K, Liao, W, Linares, C, Meis, Jf, Nielsen, K, Nyazika, Tk, Pan, W, Pekmezovic, M, Polacheck, I, Posteraro, B, de Queiroz Telles F., Filho, Romeo, Orazio, Sánchez, M, Sampaio, A, Sanguinetti, M, Sriburee, P, Sugita, T, Taj Aldeen, Sj, Takashima, M, Taylor, Jw, Theelen, B, Tomazin, R, Verweij, Pe, Wahyuningsih, R, Wang, P, Boekhout, T.

مصطلحات موضوعية: Cryptococcus, cryptococcosis, diagnostics, species delimitation, taxonomy

الوصف: Cryptococcosis is a major fungal disease caused by members of the Cryptococcus gattii and Cryptococcus neoformans species complexes. After more than 15 years of molecular genetic and phenotypic studies and much debate, a proposal for a taxonomic revision was made. The two varieties within C. neoformans were raised to species level, and the same was done for five genotypes within C. gattii. In a recent perspective (K. J. Kwon-Chung et al., mSphere 2:e00357-16, 2017, https://doi.org/10.1128/mSphere.00357-16Test), it was argued that this taxonomic proposal was premature and without consensus in the community. Although the authors of the perspective recognized the existence of genetic diversity, they preferred the use of the informal nomenclature “C. neoformans species complex” and “C. gattii species complex.” Here we highlight the advantage of recognizing these seven species, as ignoring these species will impede deciphering further biologically and clinically relevant differences between them, which may in turn delay future clinical advances.

وصف الملف: ELETTRONICO

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/28875175; info:eu-repo/semantics/altIdentifier/wos/WOS:000408618500013; volume:2; issue:4; firstpage:1; lastpage:13; numberofpages:13; journal:MSPHERE; http://hdl.handle.net/11570/3112102Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85032979870; http://msphere.asm.org/content/2/4/e00238-17Test

الإتاحة: https://doi.org/10.1128/mSphere.00238-17Test
http://hdl.handle.net/11570/3112102Test
http://msphere.asm.org/content/2/4/e00238-17Test

المؤلفون: Savić, Nada, Vojnovic, Sandra, Glišić, Biljana, Crochet, Aurelien, Pavic A., Janjić G., Pekmezovic M., Opsenica, Igor, Fromm K., Nikodinovic-Runic, Jasmina, Djuran, Miloš

المصدر: European Journal of Medicinal Chemistry

الوصف: © 2018 Elsevier Masson SAS Mononuclear silver(I) complexes with 1,7-phenanthroline (1,7-phen), [Ag(NO3-O,O′) (1,7-phen-N7)2] (1) and [Ag(1,7-phen-N7)2]X, X = ClO4− (2), CF3SO3− (3), BF4− (4) and SbF6− (5) were synthesized and structurally characterized by NMR (1H and 13C), IR and UV–Vis spectroscopy and ESI mass spectrometry. The crystal structures of 1, 3 and 4 were determined by single-crystal X-ray diffraction analysis. In all these complexes, 1,7-phen coordinates to the Ag(I) ion in a monodentate fashion via the less sterically hindered N7 nitrogen atom. The investigation of the solution stability of 1–5 in DMSO revealed that they are sufficiently stable in this solvent at room temperature. Complexes 1–5 showed selectivity towards Candida spp. in comparison to bacteria, effectively inhibiting the growth of four different Candida species with minimal inhibitory concentrations (MIC) between 1.2 and 11.3 μM. Based on the lowest MIC values and the lowest cytotoxicity against healthy human fibroblasts with selectivity index of more than 30, the antifungal potential was examined in detail for the complex 1. It had the ability to attenuate C. albicans virulence and to reduce epithelial cell damage in the cell infection model. Induction of reactive oxygen species (ROS) response has been detected in C. albicans, with fungal DNA being one of the possible target biomolecules. The toxicity profile of 1 in the zebrafish model (Danio rerio) revealed improved safety and activity in comparison to that of clinically utilized silver(I) sulfadiazine.

وصف الملف: application/pdf

العلاقة: https://scidar.kg.ac.rs/handle/123456789/8533Test; 2-s2.0-85050292358

الإتاحة: https://doi.org/10.1016/j.ejmech.2018.07.049Test
https://scidar.kg.ac.rs/handle/123456789/8533Test

المؤلفون: Milisavljević, Mira, Zivković, S., Pekmezović, M., Stanković, Nada, Vojnović, Sandra, Vasiljević, Branka, Šenerović, Lidija

المصدر: Journal of Applied Microbiology

مصطلحات موضوعية: polyene macrolide, plant pathogens, human pathogens, fungi, 33-didehydroroflamycoin

الوقت: 32

الوصف: AimsThe aim of this study was to address the toxicity of recently described polyene macrolide 32, 33-didehydroroflamycoin (DDHR) on a wide range of fungal pathogens and its potential to control plant fungal diseases. Methods and ResultsThe antifungal activity of DDHR invitro was examined against common human and plant pathogenic fungi using a broth microdilution assay and a disk diffusion assay. Minimum inhibitory concentrations ranged from 125 to 35gml(-1). A radial growth inhibition assay showed that DDHR inhibited mycelia growth, inducing mycelial necrosis and affecting sporulation. During the invivo assay on apple fruits administration of DDHR 1h before fungal inoculation inhibited spreading of the infection. Importantly, DDHR exhibited no phytotoxic effects on the model plant, Capsicum annum, verified by the plant growth rate and chlorophyll content. ConclusionsDDHR inhibits growth of various plant pathogens invitro with the strongest activity against Alternaria alternata, Colletotrichum acutatum and Penicillium expansum, and protects apple fruits from decay. Significance and Impact of the StudyThis is the first report of the inhibitory effect of DDHR on important pathogenic fungal isolates. DDHR could be a good scaffold for developing new antifungal agents for fruit and vegetable protection. ; This is the peer reviewed version of the paper: Milisavljevic, M., Zivkovic, S., Pekmezovic, M., Stankovic, N., Vojnovic, S., Vasiljevic, B., & Senerovic, L. (2015). Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin. Journal of Applied Microbiology, 118(6), 1426–1434. [https://doi.org/10.1111/jam.12811Test] ; https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/jam.12811Test

العلاقة: info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173048/RS//; info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173005/RS//; info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31018/RS//; https://imagine.imgge.bg.ac.rs/handle/123456789/876Test; https://doi.org/10.1111/jam.12811Test; https://imagine.imgge.bg.ac.rs/handle/123456789/1608Test; https://imagine.imgge.bg.ac.rs/bitstream/id/6481/Peer_reviewed_version_Control_of_human_and_plant_fungal_pathogens_usingpentaene_macrolide_32_33_didehydroroflamycoin_2015.pdfTest; 2-s2.0-84929509136; 000354570600017

الإتاحة: https://doi.org/10.1111/jam.12811Test
https://imagine.imgge.bg.ac.rs/handle/123456789/1608Test
https://imagine.imgge.bg.ac.rs/bitstream/id/6481/Peer_reviewed_version_Control_of_human_and_plant_fungal_pathogens_usingpentaene_macrolide_32_33_didehydroroflamycoin_2015.pdfTest

المؤلفون: Bruno, M., Dewi, I.M.W., Matzaraki, V., Horst, R. ter, Pekmezovic, M., Rösler, B., Groh, L.A., Roring, R.J., Kumar, V., Li, Y., Carvalho, A., Netea, M.G., Latgé, J.P., Gresnigt, M.S., Veerdonk, F.L. van de

المصدر: Computational and Structural Biotechnology Journal, 19, pp. 647-663

مصطلحات موضوعية: Radboudumc 16: Vascular damage RIMLS: Radboud Institute for Molecular Life Sciences, Radboudumc 4: lnfectious Diseases and Global Health RIMLS: Radboud Institute for Molecular Life Sciences

الوصف: Contains fulltext : 235270.pdf (Publisher’s version ) (Open Access) ; Candidiasis, aspergillosis, and mucormycosis cause the majority of nosocomial fungal infections in immunocompromised patients. Using an unbiased transcriptional profiling in PBMCs exposed to the fungal species causing these infections, we found a core host response in healthy individuals that may govern effective fungal clearance: it consists of 156 transcripts, involving canonical and non-canonical immune pathways. Systematic investigation of key steps in antifungal host defense revealed fungal-specific signatures. As previously demonstrated, Candida albicans induced type I and Type II interferon-related pathways. In contrast, central pattern recognition receptor, reactive oxygen species production, and host glycolytic pathways were down-regulated in response to Rhizopus oryzae, which was associated with an ER-stress response. TLR5 was identified to be uniquely regulated by Aspergillus fumigatus and to control cytokine release in response to this fungus. In conclusion, our data reveals the transcriptional profiles induced by C. albicans, A. fumigatus, and R. oryzae, and describes both the common and specific antifungal host responses that could be exploited for novel therapeutic strategies.

العلاقة: https://repository.ubn.ru.nl//bitstream/handle/2066/235270/235270.pdfTest; https://repository.ubn.ru.nl/handle/2066/235270Test

الإتاحة: https://doi.org/10.1016/j.csbj.2020.12.036Test
https://repository.ubn.ru.nl//bitstream/handle/2066/235270/235270.pdfTest
https://repository.ubn.ru.nl/handle/2066/235270Test

المؤلفون: Bruno, M., Horst, R. ter, Pekmezovic, M., Kumar, V., Li, Y., Netea, M.G., Gresnigt, M.S., Veerdonk, F.L. van de

المصدر: Data in Brief, 35

مصطلحات موضوعية: All institutes and research themes of the Radboud University Medical Center, Radboudumc 4: lnfectious Diseases and Global Health RIMLS: Radboud Institute for Molecular Life Sciences

الوصف: Contains fulltext : 233472.pdf (Publisher’s version ) (Open Access)

العلاقة: https://repository.ubn.ru.nl//bitstream/handle/2066/233472/233472.pdfTest; https://repository.ubn.ru.nl/handle/2066/233472Test; https://doi.org/10.1016/j.dib.2021.106928Test

الإتاحة: https://doi.org/10.1016/j.dib.2021.106928Test
https://repository.ubn.ru.nl//bitstream/handle/2066/233472/233472.pdfTest
https://repository.ubn.ru.nl/handle/2066/233472Test

المؤلفون: Kuburovic N., Pasic T., Susic G., Stevanovic, Dejan, Kuburovic V., Zdravkovic M., Janicijevic Petrovic, Mirjana, Pekmezovic M.

المصدر: Patient Preference and Adherence

الوصف: Introduction: The aims of this study were to evaluate levels of health-related quality of life (HRQOL) and the presence of anxiety and depressive symptoms in children with primary immunodeficiency disease (PID) in Serbia. Materials and methods: Self- and parent-rated data from 25 children with PID were available. As controls, data from 50 children with juvenile idiopathic arthritis (JIA) and 89 healthy children were included. The Pediatric Quality of Life Inventory was used for HRQOL assessments. Anxiety symptoms were identified using the Screen for Child Anxiety-Related Emotional Disorders questionnaire, while depressive symptoms were identified using the Mood and Feeling Questionnaire. Results: Children with PID had significantly lower Pediatric Quality of Life Inventory total scores compared to children with JIA and healthy children as child-rated (P=0.02) and parent-rated (P<0.001). Specifically, they had significantly lowered emotional functioning compared to children with JIA, and social functioning compared to both children with JIA and healthy children. School functioning was significantly lower among children with PID (parent-rated only). By parent-rated responses, six (24%) out of 25 children with PID had significant anxiety symptoms, while five (20%) children had significant depressive symptoms, which was statistically higher than among children with JIA and healthy controls (P=0.05). Conclusion: HRQOL could be significantly compromised in children with PID, particularly across such psychosocial domains as emotional, social, and school. These children were also found to be at an increased risk for suffering significant anxiety and depressive symptoms. © 2014 Kuburovic et al. This work is published by Dove Medical Press Limited.

وصف الملف: application/pdf

العلاقة: https://scidar.kg.ac.rs/handle/123456789/9344Test; 2-s2.0-84897844873

الإتاحة: https://doi.org/10.2147/PPA.S58040Test
https://scidar.kg.ac.rs/handle/123456789/9344Test

المؤلفون: Arsić A., Toncev, Gordana, Drakulić S., Vesić, Katarina, Aleksic, Dejan, Marić G., Pekmezovic M.

المصدر: Vojnosanitetski Pregled

الوصف: © 2020 Inst. Sci. inf., Univ. Defence in Belgrade. All rights reserved. Background/Aim. Malignant gliomas represent a heterogenaus group of tumors. They occur in all age groups, predominatly in males in older age. The purpose of this case-control study was to examine the association between risk for developing glioma and family history of diseases. Methods. The case-control study included 100 pathologically confirmed cases of glioma at the Clinical Centre Kragujevac, Serbia, between 2015 and 2016, and 200 age- and sex-matched controls without glioma and other malignant diseases in personal and family history at the same institution. After signing the informed consent all the patients filled out an epidemiological questionnaire. Multivariate logistic regression analysies was used in statistical data processing. Results. Malignant diseases in family history were more common in the study group than in the control group [odds ratio (OR) = 1.821, 95% confidence interval (CI) 1.004–3.305; p = 0.049]. The most common malignant tumor in the study group were cancer of the uterus (7%) and colon cancer (6%), while in the control group the most common cancer were lung cancer (6%) and cancer of the uterus (7%). Diabetes mellitus in family history was more common among control individuals than among glioma patients (OR = 0.520, 95% CI = 0.271–0.995; p = 0.048). Also, our results showed that cardiovascular diseases in family history were more common in the control group than among patients of the study group (OR = 0.557, 95% CI = 0.325–0.953; p = 0.033). Conclusion. In this case-control study, we observed a statistically significant relation between family history of malignant diseases and glioma. Also, we found statistically significant inverse relation between family history of cardiovascular diseases and diabetes and glioma.

وصف الملف: application/pdf

العلاقة: https://scidar.kg.ac.rs/handle/123456789/10654Test; 2-s2.0-85096345706

الإتاحة: https://doi.org/10.2298/VSP191127001ATest
https://scidar.kg.ac.rs/handle/123456789/10654Test

المؤلفون: Sarajlija A., Kisic-Tepavcevic D., Dobrijević, Zorana, Savic-Pavicevic, Dusanka, Obradovic, Slobodan, Djuric M., Pekmezovic M.

المصدر: Neuroepidemiology

الوصف: © 2015 S. Karger AG, Basel. Background: Rett syndrome (RTT) is a severe neurodevelopmental disorder that represents the second most common cause of mental retardation in females. However, incidence and prevalence of RTT are scarcely reported. Methods: A retrospective study included all patients with RTT diagnosed between 1981 and 2012 in Serbia. Estimation of incidence and prevalence was calculated on the basis of vital statistics reported by Statistical Office of Republic of Serbia. Results: From 1981 to 2012, RTT has been diagnosed in 102 girls in Serbia. Incidence of RTT in Serbia is estimated at 0.586:10,000 female live births. We estimated the prevalence of RTT in population of females younger than 19 years at 1:8,439. Death occurred in 19 patients (18.63%), with pneumonia as the most common cause. The lethal outcome by the age of 12 years could be expected for 11% of patients. The mean age at diagnosis was 3.5 years and we have confirmed a significant trend towards earlier dianosis during studied period. Conclusions: Rett syndrome incidence in Serbia is in accordance with reports from other countries. Serbian RTT patients have increased risk for early death when compared to patients in more developed countries, most commonly due to pneumonia. There was significant trend towards early diagnosis of RTT in Serbia over recent decades.

وصف الملف: application/pdf

العلاقة: https://scidar.kg.ac.rs/handle/123456789/12215Test; 2-s2.0-84925146582

الإتاحة: https://doi.org/10.1159/000369494Test
https://scidar.kg.ac.rs/handle/123456789/12215Test