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1دورية أكاديمية
المصدر: Microorganisms; Volume 10; Issue 1; Pages: 125
مصطلحات موضوعية: Actinomyces, susceptibility testing, agar dilution, E-Test
جغرافية الموضوع: agris
الوصف: Actinomyces species play an important role in the pathogenesis of oral diseases and infections. Susceptibility testing is not always routinely performed, and one may oversee a shift in resistance patterns. The aim of the study was to analyze the antimicrobial susceptibility of 100 well-identified clinical oral isolates of Actinomyces spp. against eight selected antimicrobial agents using the agar dilution (AD) and E-Test (ET) methods. We observed no to low resistance against penicillin, ampicillin-sulbactam, meropenem, clindamycin, linezolid and tigecycline (0–2% ET, 0% AD) but high levels of resistance to moxifloxacin (93% ET, 87% AD) and daptomycin (83% ET, 95% AD). The essential agreement of the two methods was very good for benzylpenicillin (EA 95%) and meropenem (EA 92%). The ET method was reliable for correctly categorizing susceptibility, in comparison with the reference method agar dilution, except for daptomycin (categorical agreement 87%). Penicillin is still the first-choice antibiotic for therapy of diseases caused by Actinomyces spp.
وصف الملف: application/pdf
العلاقة: Medical Microbiology; https://dx.doi.org/10.3390/microorganisms10010125Test
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المصدر: Dental Oral Biology and Craniofacial Research. :1-8
مصطلحات موضوعية: Periodontitis, biology, Gingival and periodontal pocket, Genotype, Biofilm, medicine, Actinomyces naeslundii, biology.organism_classification, medicine.disease, Dental plaque, Chronic periodontitis, Actinomyces, Microbiology
الوصف: Actinomyces naeslundii and A. oris are dental plaque formers involved in the pathogenesis of periodontitis. The aim of the study was to investigate the clonal relationship within two oral Actinomyces populations collected from plaque of patients with chronic periodontitis. The 223 clinical strains of A. naeslundii and A. oris were isolated from biofilm samples collected supra and subgingivally from teeth with shallow (probing depth (PD) = 3-4 mm), deep (PD = 5-6 mm) and very deep (PD ≥7 mm) pockets from 20 chronic periodontitis patients. All strains were submitted to repetitive sequence-based PCR typing using DiversiLab (BioMerieux,Marcy l´Étoile, France). Seven patients harboured only unrelated (95% similarity) and unrelated isolates at different sites. Identical (>98% similarity) strains were found to be present in the subgingival shallow depths more often than in the other subgingival depths. The number of clones in individual patients varied from 2 to 17 different rep-PCR genotypes. The clonal relationship within the oral populations of A. naeslundii and A. oris in an individual was unpredictable, ranging from the presence of multiple genotypes with no clonal similarity to only two different clones supra or subgingivally at different sites.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::796fc51062e20a043b002f57bd3bd1a8Test
https://doi.org/10.31487/j.dobcr.2021.02.03Test -
3دورية أكاديميةRapid identification of oral Actinomyces species cultivated from subgingival biofilm by MALDI-TOF-MS
المؤلفون: Catalina S. Stingu, Toralf Borgmann, Arne C. Rodloff, Paul Vielkind, Holger Jentsch, Wolfgang Schellenberger, Klaus Eschrich
المصدر: Journal of Oral Microbiology, Vol 7, Iss 0, Pp 1-6 (2015)
مصطلحات موضوعية: MALDI-TOF-MS, oral Actinomyces, support vector machine, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502
الوصف: Background: Actinomyces are a common part of the residential flora of the human intestinal tract, genitourinary system and skin. Isolation and identification of Actinomyces by conventional methods is often difficult and time consuming. In recent years, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has become a rapid and simple method to identify bacteria. Objective: The present study evaluated a new in-house algorithm using MALDI-TOF-MS for rapid identification of different species of oral Actinomyces cultivated from subgingival biofilm. Design: Eleven reference strains and 674 clinical strains were used in this study. All the strains were preliminarily identified using biochemical methods and then subjected to MALDI-TOF-MS analysis using both similarity-based analysis and classification methods (support vector machine [SVM]). The genotype of the reference strains and of 232 clinical strains was identified by sequence analysis of the 16S ribosomal RNA (rRNA). Results: The sequence analysis of the 16S rRNA gene of all references strains confirmed their previous identification. The MALDI-TOF-MS spectra obtained from the reference strains and the other clinical strains undoubtedly identified as Actinomyces by 16S rRNA sequencing were used to create the mass spectra reference database. Already a visual inspection of the mass spectra of different species reveals both similarities and differences. However, the differences between them are not large enough to allow a reliable differentiation by similarity analysis. Therefore, classification methods were applied as an alternative approach for differentiation and identification of Actinomyces at the species level. A cross-validation of the reference database representing 14 Actinomyces species yielded correct results for all species which were represented by more than two strains in the database. Conclusions: Our results suggest that a combination of MALDI-TOF-MS with powerful classification algorithms, such as SVMs, provide a useful tool for the differentiation and identification of oral Actinomyces.
وصف الملف: electronic resource
العلاقة: http://www.journaloforalmicrobiology.net/index.php/jom/article/view/26110/37881Test; https://doaj.org/toc/2000-2297Test
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المؤلفون: Alexandra, Wolff, Arne C, Rodloff, Paul, Vielkind, Toralf, Borgmann, Catalina-Suzana, Stingu
المصدر: Microorganisms
مصطلحات موضوعية: E-Test, polycyclic compounds, Actinomyces, susceptibility testing, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, Article, agar dilution
الوصف: Actinomyces species play an important role in the pathogenesis of oral diseases and infections. Susceptibility testing is not always routinely performed, and one may oversee a shift in resistance patterns. The aim of the study was to analyze the antimicrobial susceptibility of 100 well-identified clinical oral isolates of Actinomyces spp. against eight selected antimicrobial agents using the agar dilution (AD) and E-Test (ET) methods. We observed no to low resistance against penicillin, ampicillin-sulbactam, meropenem, clindamycin, linezolid and tigecycline (0–2% ET, 0% AD) but high levels of resistance to moxifloxacin (93% ET, 87% AD) and daptomycin (83% ET, 95% AD). The essential agreement of the two methods was very good for benzylpenicillin (EA 95%) and meropenem (EA 92%). The ET method was reliable for correctly categorizing susceptibility, in comparison with the reference method agar dilution, except for daptomycin (categorical agreement 87%). Penicillin is still the first-choice antibiotic for therapy of diseases caused by Actinomyces spp.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::5fad23c7ca16ecc08a1d967a6fc51a2fTest
https://pubmed.ncbi.nlm.nih.gov/35056574Test -
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المصدر: International Journal of Medical Microbiology. 305:682-688
مصطلحات موضوعية: Adult, Male, Microbiology (medical), Dental Plaque, Gingival Pocket, Dental plaque, Actinomycosis, Microbiology, Prevalence, medicine, Actinomyces, Humans, Actinomyces gerencseriae, Anaerobiosis, Aged, Periodontitis, Bacteriological Techniques, biology, General Medicine, Middle Aged, biology.organism_classification, medicine.disease, Actinomyces israelii, Chronic periodontitis, Infectious Diseases, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Chronic Periodontitis, Actinomyces naeslundii, Female
الوصف: This study investigated the prevalence of Actinomyces spp. in shallow, deep and very deep pockets of patients with chronic periodontitis compared to healthy controls and correlated the results with clinical status. Twenty patients with chronic periodontitis and 15 healthy subjects were enrolled in this study. Clinical indices were recorded in a six-point measurement per tooth. From each patient samples of supra and subgingival plaque were taken separately from teeth with shallow, deep and very deep pockets. Samples of supragingival plaque and sulcular microflora were collected from the healthy subjects. All the samples were cultivated on different media at 37̊C in an anaerobic atmosphere for 7 days. All the suspect colonies were identified using a rapid ID 32 A system (bioMèrieux) and MALDI-TOF-MS analysis using an Autoflex II Instrument (Bruker Daltonics) together with in house developed identification software and a reference spectra database. A total of 977 strains were identified as Actinomyces. Actinomyces naeslundii/oris/johnsonii (430 isolates) was the most prevalent species and was found in all patients and in almost all of the healthy subjects. Significant differences (p=0.003) between the groups were found for Actinomyces odontolyticus/meyeri and Actinomyces israelii which were associated with periodontitis patients. Actinomyces dentalis was found in higher percentage (p=0.015) in the periodontitis group. Actinomyces gerencseriae and Actinomyces massiliensis were significantly more often found supragingivally than subgingivally (p=0.004, p=0.022, respectively) in the periodontitis group. Whether some Actinomyces species, definitely important plaque formers, are actively involved in the pathogenicity of chronic periodontitis needs further investigation.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e3d59468ce10a17e5fad8cb5491159cTest
https://doi.org/10.1016/j.ijmm.2015.08.018Test -
6Rapid identification of oral Actinomyces species cultivated from subgingival biofilm by MALDI-TOF-MS
المؤلفون: Toralf Borgmann, Klaus Eschrich, Holger Jentsch, Catalina Suzana Stingu, Wolfgang Schellenberger, Paul Vielkind, Arne C. Rodloff
المصدر: Journal of Oral Microbiology; Vol 7 (2015)
Journal of Oral Microbiology, Vol 7, Iss 0, Pp 1-6 (2015)
Journal of Oral Microbiologyمصطلحات موضوعية: Microbiology (medical), biology, Sequence analysis, lcsh:QR1-502, biology.organism_classification, 16S ribosomal RNA, Isolation (microbiology), MALDI-TOF-MS, Microbiology, lcsh:Microbiology, lcsh:Infectious and parasitic diseases, oral Actinomyces, Matrix-assisted laser desorption/ionization, Infectious Diseases, Genotype, Original Article, support vector machine, lcsh:RC109-216, Dentistry (miscellaneous), Identification (biology), Diagnostics, basic oral microbiology, Bacteria, Actinomyces
الوصف: Background: Actinomyces are a common part of the intestinal flora of the human intestinal tract, genito-urinary system and skin. Isolation and identification of Actinomyces by conventional methods is offten difficult and time-consuming. In recent years, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has become a rapid and simple method to identify bacteria. Objective: The present study aimed to evaluate the use and value of MALDI-TOF-MS for rapid identification of different species of oral Actinomyces cultivated from subgingival biofilm. Design: Ten reference strains and 674 clinical strains were used in this study. The genotype of the reference strains and of 232 clinical strains was identified by sequence analysis of the 16S ribosomal RNA. The remaining strains were preliminary identified using biochemical methods and then subjected to MALDI-TOF-MS analysis using both similarity based analysis and classification methods (support vector machine). Results: the sequence analysis of 16S rRNA gene of all reference strains confirmed their previous identification. The MALDI-TOF-MS spectzra obtained from the reference strains and the other clinical strains undoubtedly identified as Actinomyces by 16S rRNA were used to create the mass spectra reference database. Already a visual inspection of the mass spectra of different species reveals both, similarities and differences. However, the difference between them are not large enough to allow reliable differentiation of them by similarity analysis. therefore, classification methods were applied as an alternative approach for differentiation and identification of Actinomyces at the species level. A cross-validation of the reference database representing 14 Actinomyces species yielded correct results for all species which were represented by more than 2 strains in the database. Conclusion: Our results suggests that a combination of MALDI-TOF-MS with powerful classification algorithms, like support vector machines, provides a useful tool for the differentiation and identification of oral Actinomyces. Keywords: MALDI-TOF-MS, oral Actinomyces , support vector machine
وصف الملف: application/pdf; text/html; application/epub+zip; text/plain
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56b29688fd4a0fa7d4f29fda52b7d901Test
https://doi.org/10.3402/jom.v7.26110Test -
7دورية أكاديمية
المؤلفون: Catalina S. Stingu, Toralf Borgmann, Arne C. Rodloff, Paul Vielkind, Holger Jentsch, Wolfgang Schellenberger, Klaus Eschrich
المساهمون: The Pennsylvania State University CiteSeerX Archives
الوصف: Background: Actinomyces are a common part of the residential flora of the human intestinal tract, genitourinary system and skin. Isolation and identification of Actinomyces by conventional methods is often difficult and time consuming. In recent years, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has become a rapid and simple method to identify bacteria. Objective: The present study evaluated a new in-house algorithm using MALDI-TOF-MS for rapid identification of different species of oral Actinomyces cultivated from subgingival biofilm. Design: Eleven reference strains and 674 clinical strains were used in this study. All the strains were preliminarily identified using biochemical methods and then subjected to MALDI-TOF-MS analysis using both similarity-based analysis and classification methods (support vector machine [SVM]). The genotype of the reference strains and of 232 clinical strains was identified by sequence analysis of the 16S ribosomal RNA (rRNA). Results: The sequence analysis of the 16S rRNA gene of all references strains confirmed their previous identification. The MALDI-TOF-MS spectra obtained from the reference strains and the other clinical strains undoubtedly identified as Actinomyces by 16S rRNA sequencing were used to create the mass spectra reference database. Already a visual inspection of the mass spectra of different species reveals both similarities
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