المؤلفون: Muchtaridi, Muchtaridi¹ (AUTHOR), Setyawati, Luthfi¹ (AUTHOR), Islamiaty, Risda¹ (AUTHOR), Lie, Kevin¹ (AUTHOR), Nurhidayah, Wiwit¹ (AUTHOR)

المصدر: Journal of Advanced Pharmaceutical Technology & Research. Jan-Mar2020, Vol. 11 Issue 1, p6-12. 7p.

مصطلحات موضوعية: *PAPER chromatography, *RADIOCHEMICAL purification, *LIQUID chromatography, *IDENTIFICATION, *BREAST cancer, *RADIOACTIVE tracers, *THIN layer chromatography

مستخلص: Alpha-mangostin (AM) is a natural compound that has the greatest activity in breast cancer. Radiolabeling AM with technetium-99 m (Tc-99m) has a function as breast cancer radiotracer. This study is aimed to identify the purity of Tc-99m-labeled AM. The identification method was conducted by a validated radio-high-performance liquid chromatography (HPLC) to confirm the chemical purity of the compound when the thin layer of chromatography and paper chromatography were used to find out the radiochemical purity (RCP). The validated radio-HPLC method obtained was C18 column with methanol:water (90:10) as the mobile phase and ultraviolet (243 nm) tandem radioactive detector (Gabi Star). The result showed that the RCP was 70.6% ± 2.87%. The analytical method met the validation criteria according to ICH Q2 (R1); thus, it could be applied in the identification. Unfortunately, the99mTc-AM identification using radio-HPLC showed that the expected complex was not yet formed perfectly because of chemical impurities. [ABSTRACT FROM AUTHOR]

المؤلفون: Iwashina, Tsukasa, Devkota, Hari Prasad, Mizuno, Takayuki

المصدر: Chemistry of Natural Compounds; May2023, Vol. 59 Issue 3, p543-545, 3p

مصطلحات موضوعية: SCIENCE museums, GLUCURONIC acid, PAPER chromatography, CYANIDIN, FLAVONES

مستخلص: LC-MS I m/z i : 463 [M + H] SP + sp , 461 [M - H] SP - sp (luteolin + 1 mol glucuronic acid), 287 [M - 176 + H] SP + sp , 285 [M - 176 - H] SP - sp (luteolin). As a result, we isolated and characterized nine flavonoids, i.e. 6-hydroxyluteolin 7 I O i -sophoroside ( B 1 b ), 6-hydroxyluteolin 7 I O i -glucoside ( B 2 b ), 6-hydroxyluteolin 7 I O i -sambubioside ( B 3 b ), 6-hydroxyluteolin 7 I O i -glucuronide ( B 4 b ), 6-hydroxyluteolin 7 I O i -methylglucuronide ( B 5 b ), scutellarein 7 I O i -sophoroside ( B 6 b ), scutellarein 7 I O i -glucuronide ( B 7 b ), luteolin 7 I O i -glucuronide ( B 8 b ), and pedalitin ( B 9 b ). Moreover, flower anthocyanins were isolated from 87 I Penstemon i species and identified as 3 I O i -glucoside, 3,5-di I O i -glucoside and 3 I O i -arabinosides of pelargonidin, cyanidin, and delphinidin [[9]]. [Extracted from the article]

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المؤلفون: Obreque-Slier, Elías¹ (AUTHOR), Medel-Marabolí, Marcela¹ (AUTHOR), Maldonado-Maldonado, Edio² (AUTHOR), López-Solís, Remigio O.^1,2 (AUTHOR) rlopez@med.uchile.cl

المصدر: Journal of Chromatography A. Sep2023, Vol. 1707, pN.PAG-N.PAG. 1p.

مصطلحات موضوعية: *PAPER chromatography, *SALIVA, *SALIVARY proteins, *RED wines, *ROSE wines, *WINE districts, *MOLECULAR recognition, *MOLECULAR structure

مستخلص: • A cellulose membrane serves as stationary phase for fractionation of human saliva. • Interaction between red wine and saliva was revealed by a dichromatic analysis. • A non-diffusible salivary fraction showed the highest affinity for wine components. • Diffusion of a diffusible salivary fraction declines due to interaction with wine. • A point of equivalence occurs when a wine/saliva mix shows no diffusible fraction. In-mouth interaction of red wine compounds with salivary proteins is a primary event allegedly responsible for eliciting the mouth-feel sensation of astringency. Those interactions have been currently associated with precipitation of salivary protein/polyphenol complexes. However, such single physicochemical evidence for interaction does not account for the complexity of astringency. This study aimed to develop a paper chromatography method to assess interactions between red wine and the salivary protein fraction using stepwise series of red wine/saliva binary mixtures from 100% wine to 100% saliva ("Alpha and Omega series"). Aliquots of each one of the mixtures were spotted on a cellulose membrane to scrutinize independently the distribution areas of wine components (naturally pink-colored) and salivary protein (stained blue in Coomassie Brilliant R-250). This double target detection revealed interactions between saliva and red wine components along most of the quantitative Alpha and Omega series, a point of equivalence corresponding to maximum interactivity for both complex reactants and a non-diffusible sub-fraction of saliva displaying the highest interactivity. The results indicate a novel way to assess quantitatively physicochemical interactions between red wines and human saliva but also provide new lights to approach the identification of molecular salivary structures involved in triggering astringency. [ABSTRACT FROM AUTHOR]

المؤلفون: Karayel, E., Ocak, M., Birteksöz Tan, A. Seher

مصطلحات موضوعية: infection imaging, Tc-99m-HMPAO, Tc-99mHMPAO-labeled leukocytes, antibiotic agent, hexamethylpropylene amine oxime technetium tc 99m, insulin, Article, biochemical analysis, blood pressure, erythrocyte, flow cytometry, hemocytometry, human, image quality, leukocyte, leukocyte count, leukocyte labeling, leukocyte scintigraphy, lymphocyte, lymphocyte percentage, microbiological examination, neutrophil percentage, paper chromatography, pH, purification, quality control, radiation protection, radiochemistry, radiolabeling, scintigraphy

الوصف: Objectives: The aim of this study is to evaluation of Tc-99m-hexamethylpropyleneamineoxime (HMPAO)-labeled leukocytes in terms of radiochemical, biochemical, and microbiological quality controls and to examine the effect of leukocyte numbers of the blood obtained from patients and the medications currently used by the patients on the radiochemical yields of Tc-99m-HMPAO-labeled leukocytes, and imaging quality was evaluated. Methods: Thirty paients were included in our study who applied to Istanbul University-Cerrahpasa, Cerrahpasa Faculty of Medicine, Department of Nuclear Medicine for Tc-99m-HMPAO-labeled leukocyte scintigraphy. Devices and chemicals used in the preparation of Tc-99m-HMPAO-labeled laukocytes were compared with other nuclear medicine clinics. Tc-99m-HMPAO-labeled leukocytes were evaluated in terms of radiochemical, biochemical, and microbiological quality controls. The effect of leukocyte numbers of the blood obtained from patients and the medications currently used by the patients on the radiochemical yields of Tc-99m-HMPAO-labeled leukocytes and imaging quality was evaluated. Results: The pH range of Tc-99m-HMPAO was 6-8 and the radiochemical purity was 90±2.04% (n=30), the radiochemical yield of Tc-99m-HMPAO-labeled leukocytes was 51±2.18% (n=30), the radiolabeling yield of Tc-99m-HMPAO-labeled leukocyte increased as the amount of white blood cell in the blood increased and whether the patients used any antibiotic, blood thinners, insulin and blood pressure medications did not affect the radiolabeling yield of Tc-99m-HMPAO-labeled leukocytes. The number of erythrocytes were removed at a rate of >99% in LPR by starch solution (6% HES; in the hemocytometric examination of Tc-99m-HMPAO-labeled leukocytes performed zeroth and 4th h, living/dead cell ratio was found 97.5% and the product was sterile. Conclusion: Tc-99m-HMPAO was labeled with leukocytes successfully, and Tc-99m-HMPAO-labeled leukocytes was safely injected to the patients as sterile without loss of vitality and aggregation. © ...

وصف الملف: application/pdf

العلاقة: Molecular Imaging and Radionuclide Therapy; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://doi.org/10.4274/mirt.galenos.2022.77598Test; https://search.trdizin.gov.tr/yayin/detay/1175194Test; https://hdl.handle.net/20.500.12831/13076Test; 32; 28; 34; WOS:001006704400001; 2-s2.0-85149465327; 1175194

الإتاحة: https://doi.org/10.4274/mirt.galenos.2022.77598Test
https://doi.org/20.500.12831/13076Test
https://search.trdizin.gov.tr/yayin/detay/1175194Test
https://hdl.handle.net/20.500.12831/13076Test

المؤلفون: Компанцева, Евгения Владимировна, Саушкина, Анна Степановна

المصدر: chemistry of plant raw material; No 3 (2023); 27-45 ; Химия растительного сырья; № 3 (2023); 27-45 ; 1029-5143 ; 1029-5151

مصطلحات موضوعية: plant raw materials, hydroxycinnamic acids, thin-layer chromatography, paper chromatography, растительное сырье, гидроксикоричные кислоты, тонкослойная хроматография, бумажная хроматография

الوصف: Hydroxycinnamic acids (HCC) are one of the types of biologically active compounds (BAC) synthesized by plants. Along with carbohydrates and proteins, HCCs are the most common compounds in the plant world and are found in almost all higher plants in free form, as part of esters and glycosides. Although the pharmacological activity of most HCCs has not been studied enough yet, however, its spectrum is quite wide. Many wild plants, which are currently used mainly in ethnoscience and traditional medicine, are cheap, affordable raw materials that are promising for introduction into the medical and pharmaceutical practice of our country as potential medicines. Therefore, the search for new plant species that are interesting for detailed study and the creation of modern medicines containing HCC on their basis is an scientific research area of current interest. The review is devoted to the study and systematization of paper and thin-layer chromatography methods used to determine the composition of HCC in plant raw materials growing on the territory of the Russian Federation as a potential raw material base of polyphenolic compounds. The review uses 80 sources of domestic scientific literature for the period from 2007 to 2022 inclusive, which show the results of the use of thin-layer (TLC) and paper (PC) chromatography for the study, identification and standardization of plant raw materials by the content of hydroxycinnamic acids (HCC). The research method used was a retrospective information and analytical analysis of the of Russian scientific literature sources. ; Гидроксикоричные кислоты (ГКК) – один из видов биологически активных соединений (БАС), синтезируемых растениями. Наряду с углеводами и белками ГКК – самые распространенные соединения в растительном мире и содержатся практически во всех высших растениях в свободном виде, в составе сложных эфиров и гликозидов. Хотя фармакологическая активность большинства ГКК изучена пока недостаточно, однако ее спектр достаточно широк. Многие дикорастущие растения, которые в ...

وصف الملف: application/pdf

العلاقة: http://journal.asu.ru/cw/article/view/12090/11441Test; http://journal.asu.ru/cw/article/view/12090Test

الإتاحة: https://doi.org/10.14258/jcprm.20230312090Test
http://journal.asu.ru/cw/article/view/12090Test

المؤلفون: Bhatia, Nisha, Vatsa, Rakhee, Dhingra, Vandana K., Dhawan, Devinder Kumar, Chadha, Vijayta Dani

المصدر: World Journal of Nuclear Medicine; 2022, Vol. 21 Issue 4, p314-319, 6p

مصطلحات موضوعية: RADIOCHEMICAL purification, QUALITY control, PAPER chromatography, RADIOLABELING, RADIOISOTOPES

مستخلص: Objective The aim of this study is to establish a method for the fractionation of tetrofosmin cold kit under different storage conditions and to optimize an alternate chromatography method from the reference method to test radiochemical purity (RCP). Materials and Methods Tetrofosmin cold kit vial was fractionated aseptically in six equal fractions and stored in vials and syringes. To test the stability of the reconstituted solution for a longer duration, the mother vials and syringes were stored at two different temperatures, that is, at 4°C and at −20°C till further used. Radiolabeling of fractionated tetrofosmin was performed as per the standard labeling protocol. Radionuclide purity, radioassay, and pH were tested. Radiolabeling efficiency and RCP were determined by paper chromatography. Results Radionuclide purity of eluate was greater than 99.9%. The pH of technetium-99m (Tc-99m) eluate and Tc-99m tetrofosmin was between 4.5–7.5 and 7.5–9.5, respectively. The deviation in the radioactivity during all measurements was less than 1%. The kits fractioned in glass vials resulted in higher radiolabeling yield and RCP as compared with kits fractionated in syringes. The RCP of glass vial versus syringe was observed to be greater than 95 versus 90% and 95 versus 80% at −20°C and 4°C, respectively. Conclusion Tetrofosmin kit can be used in a cost-effective manner by fractionation. One tetrofosmin vial can be used in six fractions for up to 15 days when stored at −20°C and 4°C freezer temperature. The alternative method to check the RCP of Tc-99m tetrofosmin is safer and less time consuming as compared with the reference method. [ABSTRACT FROM AUTHOR]

: Copyright of World Journal of Nuclear Medicine is the property of Thieme Medical & Scientific Publishers Private Limited and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Montesino, Luis Enrique Llanes, Corrales, Yuri Aguilera, Páez, Ahmed Díaz, Mazola, Yuniel Tejeda, Catasús, Judith Domínguez, Portela, Jorge Borroto

المصدر: Journal of Radioanalytical & Nuclear Chemistry; Sep2022, Vol. 331 Issue 9, p3359-3364, 6p, 1 Color Photograph, 5 Charts, 1 Graph

مصطلحات موضوعية: SILICA sand, RADIOACTIVE tracers, REDUCING agents, SOLIDS, SAND filtration (Water purification), PAPER chromatography

مستخلص: Pretreated silica sand labeling using varying concentrations of tin(II) fluoride and chloride as reducing agents and different times labeling was performed in order to develop a methodology for labeling silica sand with ^99mTc for using as solid radiotracer. Influence of different sand pretreatment parameters on the sorption yield (Rret%) was statistically evaluated. The effectiveness of the methods used to reduce pertechnetate (^99mTcO4⁻) by ascending paper chromatography was confirmed. Results show relatively high values of ^99mTc sorption yields on silica sand. It was possible to establish a methodology for obtaining solid ^99mTc labeled radiotracers in support of silica sand. [ABSTRACT FROM AUTHOR]

: Copyright of Journal of Radioanalytical & Nuclear Chemistry is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Sarshari, Behrang, Mohebbi, Seyed Reza, Ravanshad, Mehrdad, Shahrokh, Shabnam, Aghdaei, Hamid Asadzadeh

المصدر: Gastroenterology & Hepatology from Bed to Bench; Summer2022, Vol. 15 Issue 3, p225-231, 7p

مصطلحات موضوعية: STOMACH tumors, SEQUENCE analysis, BIOPSY, DNA, PAPER chromatography, MICROBIAL genetics, GASTRITIS, GENETIC variation, EPSTEIN-Barr virus, DESCRIPTIVE statistics, POLYMERASE chain reaction

مصطلحات جغرافية: IRAN

مستخلص: Aim: The current study aimed to investigate sequence variations in the C-terminus of latent membrane protein 1 (LMP1) in Epstein- Barr virus (EBV) isolates from Iranian patients with chronic gastritis or gastric cancer (GC). Background: LMP1, an essential viral oncoprotein, is the critical element in the immortalization of B cells. It contains a small twenty-four amino acid cytoplasmic N-terminal region, six transmembrane segments, and a two hundred amino acid cytoplasmic Cterminal domain. Most LMP1-mediated signal transduction events are moderated by some functional parts of the cytoplasmic Cterminal domain. Methods: Thirty-two EBV-positive biopsy tissues were obtained from patients with gastric cancer and patients with chronic gastritis. The C-terminal nucleotide sequences of LMP1 were amplified using nested-PCR and analyzed by DNA sequencing. Results Four to eight copies of the 11 repeat elements (codon 254--302) were observed in the carboxyl-terminal site of patients, but no relationship was found between the number of repeat sequences and disease status. The 30-bp deletion corresponding to codon 345--354 of the B95-8 strain was observed in 34% of isolates, and the remaining samples were non-deleted. In the gastric cancer group, a higher number of 33-bp repeats (≥5 repeats) was observed in 30-bp-deletion (100%) than in non-deleted (42%) isolates, and the difference was statistically significant. Analysis revealed that a gastritis isolate may be the result of recombination between Alaskan and China1 strains. Conclusion: Overall, the current results showed no association between C-terminal sequence variations of LMP1 and malignant or non-malignant isolate origin. [ABSTRACT FROM AUTHOR]

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المؤلفون: AL-Mosht, Salsabeel S., Al-Fandi, Mohamed G., Al-Ebbini, Lina M. K.

المصدر: Applied Physics A: Materials Science & Processing; Jun2022, Vol. 128 Issue 6, p1-16, 16p

مصطلحات موضوعية: HEPATOCELLULAR carcinoma, MULTIWALLED carbon nanotubes, ATOMIC force microscopy, PAPER chromatography, SINGLE walled carbon nanotubes, PROTEIN engineering, SCANNING electron microscopy, BIOSENSORS

مستخلص: Hepatocellular carcinoma (HCC) is one of the most common primary tumors in the liver. Diagnosis of HCC was investigated by detecting Glypican-3 (GPC3) protein using two approaches based on nanotechnology: developing a biosensor using a screen-printed electrode and designing and fabricating a paper-based biosensor using paper chromatography. The developed approaches were modified with multi-walled carbon nanotubes. Next, immobilized with anti-Glypican-3 antibodies, then deposited by native cow Bovine Serum Albumin (BSA). The characterizing techniques included scanning electron microscopy (SEM), atomic force microscopy (AFM), Fourier-transform infrared (FTIR) spectroscopy, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). Moreover, differential pulse voltammetry (DPV) was used to detect GPC3 protein in a concentration range of 0.0001 ng/ml to 100 ng/ml. In this study, screen-printed electrodes achieved high sensitivity (i.e., the limit of detection (LOD) equals 0.495 pg/ml) of GPC3 along with correlation coefficient (R²) equals 0.996. Meanwhile, paper-based electrodes attained slightly lower sensitivity (LOD of 0.597 pg/ml) and R² of 0.983. [ABSTRACT FROM AUTHOR]

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المؤلفون: Liu, Yingchao, Huang, Yuanyu, Wu, Mengxi, Kong, Siyuan, Cao, Weiqian, Li, Shunxiang, Yan, Guoquan, Liu, Baohong, Yang, Pengyuan, Zhang, Quanqing, Qiao, Liang, Shen, Huali

المصدر: ChemElectroChem; 5/13/2022, Vol. 9 Issue 9, p1-8, 8p

مصطلحات موضوعية: GLYCANS, BIOMOLECULES, SEPARATION (Technology), CELL separation, FILTER paper, PAPER chromatography, ELECTROPHORESIS

مستخلص: Microfluidic free‐flow electrophoresis (μ‐FFE) is widely used for continuous separation of charged analytes. However, μ‐FFE is limited in the separation of analytes with the same electrophoretic mobility. In this work, we developed a novel microfluidic free‐flow paper electrochromatography (μ‐FFPE) by combining μ‐FFE with filter paper‐based chromatography, enabling continuous separation of analytes through the mechanism of both μ‐FFE and paper chromatography. Numerical simulation was applied to demonstrate the advantages of adding filter paper as the stationary phase of μ‐FFE. With the filter paper, a more stable separation system was achieved. The device was applied to the separation of simple mixtures of dyes and complex mixtures of glycans. With the μ‐FFPE fractionation, much more glycans and glycan types can be identified by LC‐MS, demonstrating the value of the method in biological analysis. The μ‐FFPE can provide a new platform for the separation and analysis of various biological molecules. [ABSTRACT FROM AUTHOR]

: Copyright of ChemElectroChem is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)