المؤلفون: Papas, Marion

المساهمون: University/Department: Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals

مرشدي الرسالة: Miró Roig, Jordi, Yeste Oliveras, Marc

المصدر: TDX (Tesis Doctorals en Xarxa)

مصطلحات موضوعية: Plasma seminal, Seminal plasma, Antioxidants, Antioxidantes, Equids, Equidos, Ciències de la Salut

الوصف: El plasma seminal representa un important i actiu contribuent a la fertilitat dels mamífers. Entre els components del plasma seminal s’hi troben els antioxidants enzimàtics i no enzimàtics que modulen la producció d’espècies reactives d’oxigen. L’estrès oxidatiu, induït per un equilibri incorrecte entre els antioxidants i la producció de ROS, perjudica la funcionalitat espermàtica. D’aquesta manera, el plasma seminal representa el sistema de defensa més important quant a la prevenció dels danys que aquestes espècies d’oxigen provoquen en els espermatozoides. Tot i això, es sol recomanar l’eliminació del plasma seminal com a pas previ a la conservació dels espermatozoides, atès que la presència d’aquest fluid pot ser perjudicial per la longevitat dels gàmetes. En aquest context, l’objectiu principal d’aquesta Tesi Doctoral va ser determinar la importància dels enzims antioxidants del plasma seminal per l’estratègia reproductiva dels èquids. D’una banda, es va observar que les activitats de la superòxid dismutasa (SOD), la catalasa (CAT), la glutatió peroxidasa (GPX) i la glutatió reductasa (GSR) en el plasma seminal dels rucs eren molt més altes que en el dels cavalls. Curiosament, les activitats de SOD i GPX del plasma seminal van mostrar variacions estacionals en els rucs, però no en els cavalls. D’altra banda, i atès que s’està produint un augment de la demanda de semen congelat dels èquids i que cap estudi previ ha abordat la relació entre les activitats d’aquests quatre enzims i la capacitat dels espermatozoides de resistir a la criopreservació, els estudis segon i tercer van avaluar la relació entre les activitats dels enzims SOD, CAT, GPX i GSR, presents en el plasma seminal, i la criotolerància dels espermatozoides de ruc i cavall. Es va observar que l’activitat específica de la SOD en el plasma seminal està relacionada amb la criotolerància dels espermatozoides de rucs i cavall. Atès que estudis previs han demostrat que el plasma seminal modula la producció espermàtica de ROS en diferents espècies de mamífers, va estudiar si la presència del plasma seminal és capaç de controlar la generació espermàtica de ROS en condicions d’estrès oxidatiu induït pel peròxid d’hidrogen. Sorprenentment, es va observar que el contacte perllongat del plasma seminal amb els espermatozoides de ruc té un efecte limitat a l’hora de contrarestar l’estrès oxidatiu induït exògenament en aquests gàmetes. Finalment, la selecció d’espermatozoides, que comporta l’eliminació dels components del plasma seminal, s’està convertint en una tecnologia important dins l’àmbit de la reproducció assistida equina. Per tant, l’últim objectiu d’aquesta Tesi Doctoral va ser investigar els efectes de la centrifugació amb una sola fase/capa (SLC) mitjançant Equicoll sobre els paràmetres de qualitat del espermatozoides de ruc i les seves interaccions amb el tracte reproductor femení. Tot i que la selecció amb Equicoll va millorar els percentatges de viabilitat espermàtica després de la selecció, l’impacte sobre els paràmetres de motilitat va ser molt limitat. A més, l’extracció dels components del plasma seminal mitjançant Equicoll va augmentar la fagocitosi dels espermatozoides per part dels neutròfils, la qual cosa confirma que el plasma seminal modula la interacció entre els gàmetes masculins i aquestes cèl·lules immunitàries. En conclusió, aquesta Tesi Doctoral indica que, encara que les activitats dels antioxidants del plasma seminal difereixen entre cavalls i rucs, la SOD és, en ambdues espècies, un bon predictor de la capacitat dels espermatozoides de resistir a la criopreservació. A més, aquesta Tesi Doctoral també ha demostrat que el paper modulador del plasma seminal és més evident quant a la interacció entre els espermatozoides de burro i els neutròfils, que respecte a l’estrès oxidatiu induït en els gàmetes masculins d’aquesta espècie pel peròxid d’hidrogen.

الوصف (مترجم): El plasma seminal representa un importante y activo contribuyente para la fertilidad de los mamíferos. Entre los componentes del plasma seminal se encuentran los antioxidantes enzimáticos y no enzimáticos que modulan la producción de especies reactivas de oxígeno. El estrés oxidativo, inducido por un equilibrio incorrecto entre los antioxidantes y la producción de ROS, perjudica las funciones del espermatozoide. De esta manera, el plasma seminal representa el sistema de defensa más importante capaz de prevenir los daños que dichas especies reactivas de oxígeno pueden provocar en los espermatozoides. A pesar de ello, se suele recomendar la eliminación del plasma seminal como paso previo a la conservación de los espermatozoides, ya que la presencia de este fluido puede ser perjudicial para la longevidad de los gametos. El objetivo principal de esta Tesis Doctoral fue determinar la importancia de los enzimas antioxidantes del plasma seminal en la estrategia reproductiva de los equinos. Se observó que las actividades de la superóxido dismutasa (SOD), la catalasa (CAT), la glutatión peroxidasa (GPX) y la glutatión reductasa (GSR) en el plasma seminal de burros eran mucho más altas que en el de caballos. Curiosamente, las actividades de SOD y GPX en el plasma seminal mostraron variaciones estacionales en los burros, pero no en los caballos. Por otro lado, debido a que la demanda de semen congelado en la industria equina está aumentando, y que ningún estudio previo abordó la relación entre las actividades de estos cuatro enzimas y la capacidad de los espermatozoides de resistir a la congelación, los estudios segundo y tercero evaluaron la relación entre las actividades de SOD, CAT, GPX y GSR en el plasma seminal fresco y la criotolerancia de los espermatozoides de burro y caballo. Se encontró que la actividad específica del SOD en el plasma seminal está relacionada con una mejor tolerancia de los espermatozoides a la congelación tanto en caballos como en burros. Basándose en estos resultados. Dado que otros estudios previos han observado que el plasma seminal modula la producción de ROS por parte de los espermatozoides en distintas especies de mamíferos, se estudió si la presencia de plasma seminal es capaz de controlar la generación espermática de ROS en condiciones de estrés oxidativo inducido por la adición de peróxido de hidrógeno. Sorprendentemente, se observó que el contacto prolongado de los espermatozoides de burro con el plasma seminal tiene un efecto compensatorio limitado en cuanto a los respuesta de aquéllos al estrés oxidativo inducido exógenamente. Por último, la selección de los espermatozoides, que comporta la eliminación de los componentes del plasma seminal, se está convirtiendo en una tecnología prominente en el ámbito de la reproducción asistida equina. Por consiguiente, se investigó los efectos de la centrifugación con una sola fase/capa (SLC) mediante Equicoll sobre los parámetros de calidad de los espermatozoides de burro y sus interacciones con el tracto reproductivo femenino. Aunque la selección con Equicoll mejoró los porcentajes de viabilidad espermática después de la selección, el impacto sobre los parámetros de motilidad fue mínimo. Además, la extracción de los componentes del plasma seminal mediante Equicoll aumentó la fagocitosis de los espermatozoides por los neutrófilos. En conclusión, aunque las actividades de los antioxidantes seminales difieren entre caballos y burros, la SOD parece ser, en ambas especies, un buen predictor de la capacidad de los espermatozoides en resistir a la congelación. Además, se demostró que el papel modulador del plasma seminal es más evidente respecto a la interacción entre los espermatozoides de burro y los neutrófilos, que en cuanto a la respuesta de aquéllos al estrés oxidativo inducido por el peróxido de hidrógeno.
Seminal plasma represents an important and active contributor to mammalian fertility. Specific components of seminal plasma are adsorbed onto the surface of ejaculated spermatozoa while they pass through the male reproductive tract and provide sperm cells with the ability to reach and fertilize the oocyte. Among the components of seminal plasma, there are enzymatic and non-enzymatic antioxidants that modulate the production of reactive oxygen species (ROS), which are by-products generated during mitochondrial electron transport. Oxidative stress, which is induced by an incorrect balance between antioxidants and ROS production, impairs sperm function. In this context, seminal plasma represents the most important defense system able to prevent the damages that these species exert upon sperm cells. In spite of this, removal of seminal plasma is usually recommended prior to sperm storage, cooled or cryopreserved, as the presence of this fluid may be detrimental for sperm longevity. Against this background, the main aim of this Dissertation was to determine the relevance of the antioxidant enzymes present in seminal plasma for equine reproduction strategies. On the one hand, and as a first objective of this work, we observed much higher activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GSR) in jackass seminal plasma than in that of stallions. Since equids are seasonal breeders, this study also investigated season influence on the antioxidant composition of seminal plasma. Interestingly, activities of SOD and GPX in seminal plasma showed seasonal variations in jackasses but not in stallions. On the other hand, because the demand of cryopreserved semen in the equine industry is increasing and no study had previously addressed whether the activities of these four enzymes are related to sperm cryotolerance, the second and third study evaluated the relationship between SOD, CAT, GPX and GSR activities in fresh seminal plasma and the sperm ability to withstand cryopreservation in these two species. It was found that the specific SOD activity in seminal plasma is related to sperm cryotolerance in both stallions and jackasses. Since seminal plasma has been reported to modulate ROS balance in several mammalian species, this Dissertation also investigated how the presence of seminal plasma modulates ROS generation by jackass spermatozoa under oxidative stress induced exogenously by hydrogen peroxide. With regard to these results, however, prolonged contact of seminal plasma was found to have limited effect against oxidative stress induced exogenously to jackass spermatozoa. Finally, sperm selection is getting prominent in equine assisted reproduction technologies and also leads to the removal of seminal plasma components. The last objective of this study aimed to investigate the effects of single layer centrifugation (SLC) with Equicoll on jackass sperm parameters and its interactions with the female reproductive tract. While SLC-Equicoll was found to increase the percentages of viable spermatozoa after selection, the impact on sperm motility parameters was marginal. In addition, removal of seminal plasma components through SLC-Equicoll increased sperm phagocytosis by polymorphonuclear neutrophils (PMNs), thereby confirming that seminal plasma modulates the interaction between sperm and PMNs. In conclusion, this Dissertation indicates that although the activities of seminal plasma antioxidants differ between stallions and jackasses, SOD appears to be a good predictor of sperm cryotolerance in these two species. In addition, this Dissertation has also demonstrated, for the first time, that the modulating role of seminal plasma with regard to the interaction between jackass spermatozoa and PMNs is more apparent than that observed when oxidative stress is induced by hydrogen peroxide.

وصف الملف: application/pdf

الوصول الحر: http://hdl.handle.net/10803/670186Test

المؤلفون: Hedia, Mohamed, Angel-Velez, Daniel, Papas, Marion, Peere, Sofie, Gerits, Ilse, De Coster, Tine, Van den Branden, Emma, Govaere, Jan, Van Soom, Ann, Leroy, Jo L.M.R., Smits, Katrien

المساهمون: Voortplanting paard

مصطلحات موضوعية: BAP, IL-6, Mares, OPU-ICSI, Oxidative stress, r-ROMs, Taverne, Small Animals, Food Animals, Animal Science and Zoology, Equine

الوصف: The in vitro production of equine embryos via ovum pick-up (OPU) and intracytoplasmic sperm injection (ICSI) has increased rapidly. There is a marked effect of the individual mare on the outcome of OPU-ICSI, but little is known about the influence of the mare's health condition. This study aimed to investigate the potential associations between the concentrations of interleukin-6 (IL-6), reactive oxygen metabolites (d-ROMs), and biological antioxidant potential (BAP) in serum of oocytes' donor mares and the subsequent embryonic development. Just before OPU, a blood sample was collected from 28 Warmblood donor mares, that were subjected to a routine OPU-ICSI program. The serum concentrations of IL-6, d-ROMs, and BAP were assayed photometrically. The maturation, cleavage and blastocyst rate as well as the kinetics of blastocyst development were recorded. The average blastocyst rate was 24.68 ± 5.16% and the average concentrations of IL-6, d-ROMs, and BAP were 519.59 ± 157.08 pg/mL, 171.30 ± 4.55 carratelli units (UCARR), and 2711.30 ± 4.55 μmol/L, respectively. Serum concentrations of IL-6, d-ROMs, and BAP were not significantly different between mares yielding at least one blastocyst (552.68 ± 235.18 pg/mL, 168.36 ± 5.56 UCARR, and 2524.80 ± 159.55 μmol/L) and mares yielding no blastocysts (468.47 ± 179.99 pg/mL, 175.85 ± 7.89 UCARR, and 2999.50 ± 300.13 μmol/L, respectively). Serum concentrations of d-ROMs were significantly lower in mares with fast growing (at day 7–8 post ICSI; 148.10 ± 8.13 UCARR) compared to those with slow growing blastocysts (≥ day 9 post ICSI; 179.41 ± 4.89 UCARR; P = 0.003). Taken together, the serum concentration of IL-6, d-ROMs, and BAP do not determine the mare's ability to produce blastocysts in vitro. Although it may be questioned whether a single sample is representative of the mare's health status, changes in serum metabolites related to oxidative stress at the time of oocyte retrieval were linked to a delayed blastocyst development in a clinical OPU-ICSI outcome.

وصف الملف: application/pdf

العلاقة: https://dspace.library.uu.nl/handle/1874/438355Test

الإتاحة: https://dspace.library.uu.nl/handle/1874/438355Test

المؤلفون: De Coster, Tine, Van Poucke, Mario, Bogado Pascottini, Osvaldo Américo, Angel Velez, Daniel, Van den Branden, Emma, Peere, Sofie, Papas, Marion, Gerits, Ilse, Govaere, Jan, Peelman, Luc, Vermeesch, J. R., Van Soom, Ann, Smits, Katrien

المصدر: ANIMAL ; ISSN: 1751-7311 ; ISSN: 1751-732X

مصطلحات موضوعية: Veterinary Sciences, PREIMPLANTATION GENETIC DIAGNOSIS, FETAL SEX, IN-VIVO, SIZE DISTRIBUTIONS, BLASTOCYST CAPSULE, HORSE EMBRYO, BIOPSY, DNA, Assisted reproduction, ETSTY5, Horse, Preimplantation genetic testing, qPCR

الوصف: In addition to fulfilling many breeders' curiosity, equine embryonic sex determination can have a pro-found commercial impact. However, the application of currently described assays for equine embryonic sexing has rendered variable diagnosis and validation rates, with sensitivity being the main problem. In addition, while pregnancy results of in vivo-flushed equine embryos following a needle aspiration biopsy equal those of non-biopsied embryos, the effect on in vitro-produced embryos is unknown. Here, we aimed to develop a highly sensitive and specific assay for equine sex determination that can be directly performed on few embryonic cells, and to test the effect of a needle aspiration biopsy on the viability of the in vitro-produced embryo. To this end, a multiplex quantitative real-time PCR (qPCR) assay with dual-labelled probes was designed to allow the simultaneous generation of both male-specific and control fragments in a single closed-tube reaction, avoiding potential sample loss or contamination. To improve sensitivity, multicopy and polymeric genes were chosen to be specifically amplified, i.e., eight copies of Y-chromosomal ETSTY5 as male-specific and four autosomal UBC monomers as control fragment. Specificity was enhanced by the equine-specific character of ETSTY5 and by using dual-labelled probes. The assay was optimised with equine male and female genomic DNA and demonstrated a 100% accuracy and a >95% qPCR efficiency down to 10 pg of DNA. The assay was subsequently applied to determine the sex of 44 in vitro-produced embryos, collecting trophectoderm biopsies by means of a needle aspiration biopsy and herniating cells. Of all trophectoderm biopsies and herniating cell samples (n = 54), 87% could be diagnosed. Assay results were validated on a second sample obtained from the biopsied embryo (n = 18) or, by ultrasound-based sex determination of the foetus (n = 7) following the transfer of the biopsied embryo to a recipient mare, with about half of the embryos being fillies and colts. The needle ...

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/01HNYPAPYCRTK7T7Q0T7EBZ39CTest; http://hdl.handle.net/1854/LU-01HNYPAPYCRTK7T7Q0T7EBZ39CTest; http://doi.org/10.1016/j.animal.2023.100952Test; https://biblio.ugent.be/publication/01HNYPAPYCRTK7T7Q0T7EBZ39C/file/01HNYPC4QJBQWAGEBTSCDV191GTest

الإتاحة: https://doi.org/10.1016/j.animal.2023.100952Test
https://biblio.ugent.be/publication/01HNYPAPYCRTK7T7Q0T7EBZ39CTest
http://hdl.handle.net/1854/LU-01HNYPAPYCRTK7T7Q0T7EBZ39CTest
https://biblio.ugent.be/publication/01HNYPAPYCRTK7T7Q0T7EBZ39C/file/01HNYPC4QJBQWAGEBTSCDV191GTest

المؤلفون: Peere, Sofie, van Den Branden, Emma, Papas, Marion, Gerits, Ilse, Smits, Katrien, Govaere, Jan

المصدر: Equine Veterinary Journal; Jul2024, Vol. 56 Issue 4, p650-659, 10p

مستخلص: Twin gestation in the mare is undesirable and can have disastrous consequences. As in many cases, the key to success in twin management lies in a thorough follow‐up and accurate recording of clinical findings in the pre‐breeding examination. A pregnancy diagnosis in the mobility phase is imperative for a good outcome in the event of twin reduction. If a twin gestation is not diagnosed during this early pregnancy stage, several other procedures exist for managing post‐fixation twins (>16 days) with varying degrees of success. Most twin pregnancies are the result of multiple ovulations (dizygotic twins). However, monozygotic twins are also sporadically diagnosed, due to the increasing number of transferred in vitro produced equine embryos. In these cases, the most optimal treatment strategy still needs to be determined. This review provides an overview of the various twin reduction techniques described with the expected prognosis as well as of some less reported techniques with their results. In addition, physiological events and the reduction techniques are demonstrated to the user in virtual 3‐dimensional illustrations. [ABSTRACT FROM AUTHOR]

: Copyright of Equine Veterinary Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Eerdekens, Anniek, Papas, Marion, Damiaans, Bert, Martens, Luc, Govaere, Jan, Joseph, Wout, Deruyck, Margot

المصدر: Equine Veterinary Journal ; ISSN 0425-1644 2042-3306

الوصف: Background To seek appropriate veterinary attention for horses with colic, owners must recognise early signs. Direct observation of horse behaviour has several drawbacks: it is time‐consuming, hard to see subtle and common behavioural signs, and is based on intuition and subjective decisions. Due to recent advances in wearables and artificial intelligence, it may be possible to develop diagnostic software that can automatically detect colic signs. Objectives To develop a software algorithm to aid in the detection of colic signs and levels of pain. Study design In vivo experiments. Methods Transient colic was induced in eight experimental mares with luteolytic doses of prostaglandin. Veterinarians observed the horses before and throughout the interventions and assigned pain scores which were used to separate colic episodes into none (pain score ≤5), level 1 (pain score 6–10) or level 2 (pain score ≥11). Accelerometric data and videos were collected throughout the experiments and using accelerometric data, the horse's behaviour was classified into normal and 10 pain‐related behaviours and an activity index was calculated. Models were designed that utilised behaviour and activity index characteristics both detecting the presence of colic and assessing its severity. To determine the accuracy of the model, the ground truth, that is the veterinarians' observation of colic signs and assessment of pain level, was compared with the automatic detection system. Results The cross‐validation analysis demonstrated an accuracy of 91.2% for detecting colic and an accuracy of 93.8% in differentiating between level 1 colic and level 2 colic. The model was able to accurately classify 10 pain‐related behaviours and distinguish them from normal behaviour with a high accuracy. Main limitations We included a limited number of horses with severe pain related behaviours in the dataset. This constraint affects the accuracy of categorising colic severity rather than limiting the algorithms' capacity to identify early colic signs. ...

الإتاحة: https://doi.org/10.1111/evj.14069Test

المؤلفون: Catalán, Jaime, Yáñez-Ortiz, Iván, Gacem, Sabrina, Papas, Marion, Bonet, Sergi, Rodríguez Gil, Joan Enric, Yeste Oliveras, Marc, Miró, Jordi

المساهمون: Agencia Estatal de Investigación, Ministerio de Economía y Competitividad (Espanya)

المصدر: Animals, 2021, vol. 11, núm. 1, p. 122 ; Articles publicats (D-B) ; Catalán, Jaime Yáñez-Ortiz, Iván Gacem, Sabrina Papas, Marion Bonet, Sergi Rodríguez Gil, Joan Enric este Oliveras, Marc Miró, Jordi 2021 The effects of red light on mammalian sperm rely upon the color of the recipient and the medium used Animals 11 1 122

مصطلحات موضوعية: Espermatozoides, Fecunditat, Spermatozoa, Fertility

الوصف: Previous research has determined that irradiation of mammalian sperm with red light increases motility, mitochondrial activity, and fertilization capacity. In spite of this, no study has considered the potential influence of the color of the straw and the extender used. Therefore, this study tests the hypothesis that the response of mammalian sperm to red light is influenced by the color of the straw and the turbidity/composition of the extender. Using the horse as a model, 13 ejaculates from 13 stallions were split into two equal fractions, diluted with Kenney or Equiplus extender, and stored at 4 °C for 24 h. Thereafter, each diluted fraction was split into five equal aliquots and subsequently packed into 0.5-mL straws of red, blue, yellow, white, or transparent color. Straws were either nonirradiated (control) or irradiated with a light-dark-light pattern of 3-3-3 (i.e., light: 3 min, dark: 3 min; light: 3 min) prior to evaluating sperm motility, acrosome and plasma membrane integrity, mitochondrial membrane potential, and intracellular ROS and calcium levels. Our results showed that irradiation increased some motion variables, mitochondrial membrane potential, and intracellular ROS without affecting the integrities of the plasma membrane and acrosome. Remarkably, the extent of those changes varied with the color of the straw and the extender used; the effects of irradiation were more apparent when sperm were diluted with Equiplus extender and packed into red-colored straws or when samples were diluted with Kenney extender and packed into transparent straws. As the increase in sperm motility and intracellular ROS levels was parallel to that of mitochondrial activity, we suggest that the impact of red light on sperm function relies upon the specific rates of energy provided to the mitochondria, which, in turn, vary with the color of the straw and the turbidity/composition of the extender ; J.C. was funded by the National Agency for Research and Development (ANID), Ministry of Education, Chile (Scheme: Becas ...

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العلاقة: info:eu-repo/semantics/altIdentifier/eissn/2076-2615; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R/ES/MEJORA DEL RENDIMIENTO REPRODUCTIVO DEL SEMEN REFRIGERADO Y CONGELADO/DESCONGELADO DE PORCINO Y BOVINO MEDIANTE EL USO DE LA FOTOESTIMULACION/; info:eu-repo/grantAgreement/MINECO//RYC-2014-15581/ES/RYC-2014-15581/; http://hdl.handle.net/10256/18965Test

الإتاحة: https://doi.org/10.3390/ani11010122Test
http://hdl.handle.net/10256/18965Test

المؤلفون: Papas, Marion, Govaere, Jan, Peere, Sofie, Gerits, Ilse, Van de Velde, Margot, Angel Velez, Daniel, De Coster, Tine, Van Soom, Ann, Smits, Katrien

المصدر: ANIMALS ; ISSN: 2076-2615

مصطلحات موضوعية: Veterinary Sciences, anti-mullerian hormone, mare, OPU, ICSI, ANTRAL FOLLICLE COUNT, OVARIAN RESERVE, EMBRYO PRODUCTION, ENDOCRINE MARKER, BLOOD-SERUM, AMH, BLASTOCYST, QUALITY, FSH, AGE

الوصف: Simple Summary The in vitro production of equine embryos, using ovum pick-up (OPU) and intracytoplasmic sperm injection (ICSI), is gaining popularity for breeding sport horses. However, the results of this complicated procedure are variable and hard to predict. Therefore, the aim of this study was to evaluate anti-Mullerian hormone (AMH), a factor which has been linked to reproductive success in human and cattle, as an indicator of the OPU-ICSI outcome in horses. Therefore, for 103 mares subjected to commercial OPU-ICSI, the AMH level was measured in the blood at the moment of OPU and linked to the number of oocytes and embryos produced by ICSI. We found that mares with a high level of AMH gave rise to a better oocyte collection and a higher number of embryos. However, since mares with a low AMH value could also produce embryos, a single measurement of the AMH in the blood is not sufficient as an independent predictor of the OPU-ICSI outcome in the horse. Anti-Mullerian hormone (AMH) reflects the population of growing follicles and has been related to mammalian fertility. In the horse, clinical application of ovum pick-up and intracytoplasmic sperm injection (OPU-ICSI) is increasing, but results depend largely on the individuality of the mare. The aim of this study was to assess AMH as a predictor for the OPU-ICSI outcome in horses. Therefore, 103 mares with a total follicle count above 10 were included in a commercial OPU-ICSI session and serum AMH was determined using ELISA. Overall, the AMH level was significantly correlated with the number of aspirated follicles and the number of recovered oocytes (p < 0.001). Mares with a high AMH level (>= 2.5 mu g/L) yielded significantly greater numbers of follicles (22.9 +/- 1.2), oocytes (13.5 +/- 0.8), and blastocysts (2.1 +/- 0.4) per OPU-ICSI session compared to mares with medium (1.5-2.5 mu g/L) or low AMH levels (<1.5 mu g/L), but no significant differences in blastocyst rates were observed. Yet, AMH levels were variable and 58% of the mares with low AMH ...

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/8715153Test; http://hdl.handle.net/1854/LU-8715153Test; http://dx.doi.org/10.3390/ani11072004Test; https://biblio.ugent.be/publication/8715153/file/8715155Test

الإتاحة: https://doi.org/10.3390/ani11072004Test
https://biblio.ugent.be/publication/8715153Test
http://hdl.handle.net/1854/LU-8715153Test
https://biblio.ugent.be/publication/8715153/file/8715155Test

المؤلفون: Catalán, Jaime, Yánez-Ortiz, Iván, Gacem, Sabrina, Papas, Marion, Bonet, Sergi, Rodríguez Gil, Joan Enric, Yeste Oliveras, Marc, Miró, Jordi

مصطلحات موضوعية: Cavalls, Espermatozoides, Horse, Sperm, Red light irradiation, Extender, Straw

الوصف: Altres ajuts: PFCHA/2017/72180128 ; Altres ajuts: PBIP/CZ02-000507-2019 ; Several studies have shown that the exposure of semen to red light improves sperm quality and fertilizing ability, which could improve the efficiency of assisted reproductive techniques with irradiated semen. However, despite being considered as possible sources of variation, the effects of the color of the container (straws) or the medium have not yet been evaluated. In this study, 13 ejaculates from different stallions were split into equal fractions, diluted either with Kenney or Equiplus extender, and subsequently packed into straws of five different colors. After storage at 4 °C for 24 h, the sperm were irradiated and different variables, including sperm motility, plasma membrane integrity, and mitochondrial membrane potential, were evaluated. Our results confirm that irradiation increases some motion characteristics and mitochondrial membrane potential without affecting sperm viability and demonstrate that the effects depend on the color of the straw and the extender used. Previous research has determined that irradiation of mammalian sperm with red light increases motility, mitochondrial activity, and fertilization capacity. In spite of this, no study has considered the potential influence of the color of the straw and the extender used. Therefore, this study tests the hypothesis that the response of mammalian sperm to red light is influenced by the color of the straw and the turbidity/composition of the extender. Using the horse as a model, 13 ejaculates from 13 stallions were split into two equal fractions, diluted with Kenney or Equiplus extender, and stored at 4 °C for 24 h. Thereafter, each diluted fraction was split into five equal aliquots and subsequently packed into 0.5-mL straws of red, blue, yellow, white, or transparent color. Straws were either nonirradiated (control) or irradiated with a light-dark-light pattern of 3-3-3 (i.e., light: 3 min, dark: 3 min; light: 3 min) prior to evaluating sperm motility, acrosome and ...

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العلاقة: Ministerio de Ciencia e Innovación RYC-2014-15581; Ministerio de Ciencia e Innovación AGL2017-88329-R; Agència de Gestió d'Ajuts Universitaris i de Recerca 2017-SGR-1229; Animals; Vol. 11 (january 2021); https://ddd.uab.cat/record/236224Test; urn:10.3390/ani11010122; urn:oai:ddd.uab.cat:236224; urn:pmcid:PMC7826721; urn:pmc-uid:7826721; urn:pmid:33429933; urn:oai:pubmedcentral.nih.gov:7826721; urn:oai:egreta.uab.cat:publications/8e3ac867-aa5f-4dd6-ba5b-a49d358b28d1

الإتاحة: https://ddd.uab.cat/record/236224Test

المؤلفون: Miró, Jordi, Marín, Henar, Catalán, Jaime, Papas, Marion, Gacem, Sabrina, Yeste Oliveras, Marc

المساهمون: Agencia Estatal de Investigación, Ministerio de Economía y Competitividad (Espanya)

المصدر: International Journal of Molecular Sciences, 2020, vol. 21, núm. 9, p. 3478 ; Articles publicats (D-B) ; Miró, Jordi Marín, Henar Catalán, Jaime Papas, Marion Gacem, Sabrina Yeste Oliveras, Marc 2020 Seminal plasma, sperm concentration and sperm-PMN interaction in the donkey: an in vitro model to study endometrial inflammation at post-insemination International Journal of Molecular Sciences 21 9 3478

مصطلحات موضوعية: Ases -- Fecunditat, Donkeys -- Fertility, Ases -- Reproducció, Donkeys -- Reproduction, Inseminació artificial, Artificial insemination, Endometri, Endometrium

الوصف: In the donkey, artificial insemination (AI) with frozen-thawed semen is associated with low fertility rates, which could be partially augmented through adding seminal plasma (SP) and increasing sperm concentration. On the other hand, post-AI endometrial inflammation in the jenny is significantly higher than in the mare. While previous studies analyzed this response through recovering Polymorphonuclear Neutrophils (PMN) from uterine washings, successive lavages can detrimentally impact the endometrium, leading to fertility issues. For this reason, the first set of experiments in this work intended to set an in vitro model through harvesting PMN from the peripheral blood of jennies. Thereafter, how PMN, which require a triggering agent like formyl-methionyl-leucyl-phenylalanine (FMLP) to be activated, are affected by donkey semen was interrogated. Finally, we tested how four concentrations of spermatozoa (100 × 106, 200 × 106, 500 × 106 and 1000 × 106 spermatozoa/mL) affected their interaction with PMN. We observed that semen, which consists of sperm and SP, is able to activate PMN. Whereas there was a reduced percentage of spermatozoa phagocytosed by PMN, most remained attached on the PMN surface or into a surrounding halo. Spermatozoa not attached to PMN were viable, and most of those bound to PMN were also viable and showed high tail beating. Finally, only sperm concentrations higher than 500 × 106 spermatozoa/mL showed free sperm cells after 3 h of incubation, and percentages of spermatozoa not attached to PMN were higher at 3 h than at 1 h, exhibiting high motility. We can thus conclude that semen activates PMN in the donkey, and that the percentage of spermatozoa phagocytosed by PMN is low. Furthermore, because percentages of spermatozoa not attached to PMN were higher after 3 h than after 1 h of incubation, we suggest that PMN-sperm interaction plays an instrumental role in the reproductive strategy of the donkey ; This research was supported by the Ministry of Science and Innovation, Spain (Grants: ...

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العلاقة: info:eu-repo/semantics/altIdentifier/eissn/1422-0067; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R/ES/MEJORA DEL RENDIMIENTO REPRODUCTIVO DEL SEMEN REFRIGERADO Y CONGELADO/DESCONGELADO DE PORCINO Y BOVINO MEDIANTE EL USO DE LA FOTOESTIMULACION/; info:eu-repo/grantAgreement/MINECO//RYC-2014-15581/ES/RYC-2014-15581/; http://hdl.handle.net/10256/18086Test

الإتاحة: https://doi.org/10.3390/ijms21103478Test
http://hdl.handle.net/10256/18086Test

المؤلفون: Catalán, Jaime, Papas, Marion, Trujillo Rojas, Lina, Blanco Prieto, Olga, Bonilla Correal, Sebastián, Rodríguez Gil, Joan Enric, Miró, Jordi, Yeste Oliveras, Marc

المصدر: Frontiers In Cell And Developmental Biology, 2020, vol. 8, art.núm. 588621 ; Articles publicats (D-B) ; Catalán, Jaime Papas, Marion Trujillo Rojas, Lina Blanco Prieto, Olga Bonilla Correal, Sebastián Rodríguez Gil, Joan Enric Miró, Jordi Yeste Oliveras, Marc 2020 Red LED light acts on the mitochondrial electron chain of donkey sperm and its effects depend on the time of exposure to light Frontiers In Cell And Developmental Biology 8 Art.núm. 588621

مصطلحات موضوعية: Espermatozoides, Mitocondris, Ases -- Espermatozoides, Spermatozoa, Mitochondria, Donkeys -- Spermatozoa

الوصف: This work aimed to investigate how stimulation of donkey sperm with red LED light affects mitochondrial function. For this purpose, freshly diluted donkey semen was stimulated with red light for 1 min, 5 min and 10 min, in the presence or absence of oligomycin A (Omy A), a specific inhibitor of mitochondrial ATP synthase, or FCCP, a specific disruptor of mitochondrial electron chain. The results obtained in the present study indicated that the effects of red LED light on fresh donkey sperm function are related to changes in mitochondria function. In effect, irradiation of donkey sperm resulted in an increase in mitochondrial membrane potential, the activity of cytochrome C oxidase and the rate of oxygen consumption. In addition, in the absence of oligomycin A and FCCP, light-stimulation augmented the average path velocity (VAP) and modified the structure of motile sperm subpopulations, increasing the fastest and most linear subpopulation. In contrast, the presence of either Omy A or FCCP abolished the aforementioned effects. Interestingly, our results also showed that the effects of red light depend on the exposure time applied, as indicated by the observed differences between irradiation protocols. In conclusion, our results suggest that exposing fresh donkey sperm to red light modulates the function of their mitochondria through affecting the activity of the electron chain. However, the extent of this effect depends on the irradiation pattern and does not exclude the existence of other mechanisms, such as those related to thermotaxis

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العلاقة: info:eu-repo/semantics/altIdentifier/eissn/2296-634X; http://hdl.handle.net/10256/18774Test

الإتاحة: https://doi.org/10.3389/fcell.2020.588621Test
http://hdl.handle.net/10256/18774Test