المؤلفون: Paolo Gresele, Giuseppe Guglielmini, Maurizio Del Pinto, Paolo Calabrò, Pasquale Pignatelli, Giuseppe Patti, Vittorio Pengo, Emilia Antonucci, Plinio Cirillo, Tiziana Fierro, Gualtiero Palareti, Rossella Marcucci, START Antiplatelet Registry Group

المصدر: Scientific Reports, Vol 14, Iss 1, Pp 1-10 (2024)

مصطلحات موضوعية: Medicine, Science

الوصف: Abstract Some previous observations suggest that a low platelet count is associated with an increased risk of adverse outcomes in patients with acute coronary syndromes (ACS). However, most of the data come from post-hoc analyses of randomized controlled trials and from studies including thrombocytopenia developed during hospital stay. Our aim was to assess the impact of low platelet count at admission on cardiovascular outcomes and treatment approach in patients hospitalized for ACS in a current real-life setting in Italy. Patients admitted to Italian coronary care units for ACS were enrolled in the START-ANTIPLATELET registry. Baseline clinical characteristics and treatment at discharge were recorded. Patients were followed-up at 6 months, 1 year and yearly thereafter. Low platelet count was defined as a count at admission 100 k/µl or 100 k/µl and 30 (1.6%) 100 k/µl: 37.6 vs 41.8 months, p = 0.002; HR = 2.7, 95% CIs 1.4–5.2;

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/bb1cd95c94b44906bfe953431c75206fTest

المؤلفون: Arianna Miglio, Emanuela Falcinelli, Anna Maria Mezzasoma, Sara Busechian, Fabrizio Rueca, Paolo Gresele, Maria Teresa Antognoni

المصدر: Frontiers in Veterinary Science, Vol 11 (2024)

مصطلحات موضوعية: soluble P-selectin, platelet-derived extracellular vesicles, equine, exercise, platelet, Veterinary medicine, SF600-1100

الوصف: Physical exercise has an activating effect on platelet function that differs between trained and untrained subjects, depending on the type of exercise and training status. In humans, soluble P-selectin (sP-sel) and platelet-derived extracellular vesicles (PEVs) are considered reliable markers of in vivo platelet activation during exercise. In untrained humans, they increase after transient physical exercise, whereas long-term training induces a decrease in their resting levels due to an improved ability to adapt to hemodynamic changes. The aim of this study was to assess whether circulating levels of sP-sel and PEVs may be useful markers to explore in vivo platelet function in never-trained Thoroughbreds during their first 4 months of incremental training. A total of 29 clinically healthy, untrained Thoroughbreds (17 males and 12 females) were enrolled. All horses were trained with the same training schedule (90 days). Blood samples were collected on the day the training program began (T0), 30 days (T30), and 90 days (T90) after its incremental increase to quantify platelet count, sP-sel (horse enzyme-linked immunosorbent assay) and PEVs (flow cytometry). Statistical analysis was performed using RM one-way analysis of variance with the Geisser–Greenhouse correction. Soluble P-selectin tended to increase at T30 compared with T0, while T90 levels returned to baseline values. Significantly higher circulating levels of PEVs CD61+/AnnV+ were observed at T30 and T90 compared to baseline confirming platelet hyperactivity. The detection and quantification of sP-sel and PEVs in equine racehorses during the training period appears to be a promising tool to study exercise-induced primary hemostatic changes and may provide an important marker for exercise selection.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fvets.2024.1395423/fullTest; https://doaj.org/toc/2297-1769Test

الوصول الحر: https://doaj.org/article/4106393c01a1444ca5d7b33259563e38Test

المؤلفون: Roberto Mario Santi, Annamaria Fulghesu, Ezio Zanon, Erica De Candia, Elvira Grandone, Giancarlo Di Renzo, Claudia Succu, Valentina Tosto, Vincenzina Bruni, Paolo Gresele

المصدر: Bleeding, Thrombosis and Vascular Biology, Vol 3, Iss 1 (2024)

مصطلحات موضوعية: Abnormal uterine bleeding, adolescence, consensus paper, Diseases of the circulatory (Cardiovascular) system, RC666-701

الوصف: During menarche and adolescence, abnormal uterine bleeding (AUB) may serve as a crucial indicator of a hidden bleeding disorder. When a young woman is suspected of having a bleeding disorder, the obstetrician-gynecologist should collaborate with a hemostasis specialist to arrange the necessary laboratory testing and determine the best course of action. The Italian Society for the Study of Hemostasis and Thrombosis and the Italian Society of Gynecology and Obstetrics jointly offer recommendations on the diagnosis and treatment of AUB. They also suggest diagnostic and therapeutic pathways to decrease diagnostic delay and improve treatment effectiveness and safety.

وصف الملف: electronic resource

العلاقة: https://www.btvb.org/btvb/article/view/106Test; https://doaj.org/toc/2785-5309Test

الوصول الحر: https://doaj.org/article/22fe84ca7bd3433596f98e0e0156fb12Test

المؤلفون: Paolo Gresele

المصدر: Bleeding, Thrombosis and Vascular Biology, Vol 2, Iss 4 (2024)

مصطلحات موضوعية: Artificial intelligence, machine learning, hemostasis and thrombosis, Diseases of the circulatory (Cardiovascular) system, RC666-701

الوصف: Artificial intelligence (AI) is rapidly becoming more important in our daily lives, and it’s beginning to be used in life sciences and in healthcare. AI and machine learning (ML) models are just starting to be applied in the field of hemostasis and thrombosis, but there are already many examples of how they can be useful in basic research/pathophysiology, laboratory diagnostics, and clinical settings. This review wants to shortly explain how AI works, what have been its uses in hemostasis and thrombosis so far and what are possible future developments. Besides the great potential advantages of a correct application of AI to the field of hemostasis and thrombosis, possible risks of inaccurate or deliberately mischievous use of it must be carefully considered. A close monitoring of AI employment in healthcare and research will have to be applied over the next years, but it is expected that the appropriate employment of this new revolutionary technology will bring great advances to the medical field, including to the hemostasis and thrombosis area. The current review, addressed to non-experts in the field, aims to go through the applications of AI in the field of hemostasis and thrombosis that have been explored so far and to examine its advantages, drawbacks and future perspectives.

وصف الملف: electronic resource

العلاقة: https://btvb.org/btvb/article/view/105Test; https://doaj.org/toc/2785-5309Test

الوصول الحر: https://doaj.org/article/f107444f28ba49ec9603dcddd1496425Test

المؤلفون: Laura Goracci, Eleonora Petito, Alessandra Di Veroli, Emanuela Falcinelli, Caterina Bencivenga, Elisa Giglio, Cecilia Becattini, Edoardo De Robertis, Gaetano Vaudo, Paolo Gresele

المصدر: Platelets, Vol 34, Iss 1 (2023)

مصطلحات موضوعية: covid-19, platelet lipidomics, Diseases of the blood and blood-forming organs, RC633-647.5

الوصف: Ischemic cardiovascular and venous thromboembolic events are a frequent cause of death in severe COVID-19 patients. Platelet activation plays a key role in these complications, however platelet lipidomics have not been studied yet. The aim of our pilot investigation was to perform a preliminary study of platelet lipidomics in COVID-19 patients compared to healthy subjects. Lipid extraction and identification of ultrapurified platelets from eight hospitalized COVID-19 patients and eight age- and sex-matched healthy controls showed a lipidomic pattern almost completely separating COVID-19 patients from healthy controls. In particular, a significant decrease of ether phospholipids and increased levels of ganglioside GM3 were observed in platelets from COVID-19 patients. In conclusion, our study shows for the first time that platelets from COVID-19 patients display a different lipidomics signature distinguishing them from healthy controls, and suggests that altered platelet lipid metabolism may play a role in viral spreading and in the thrombotic complications of COVID-19.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/0953-7104Test; https://doaj.org/toc/1369-1635Test

الوصول الحر: https://doaj.org/article/58e96481cbcd4a71b9e37e00c5c65614Test

المؤلفون: Arianna Miglio, Emanuela Falcinelli, Katia Cappelli, Samanta Mecocci, Anna Maria Mezzasoma, Maria Teresa Antognoni, Paolo Gresele

المصدر: Animals, Vol 14, Iss 3, p 414 (2024)

مصطلحات موضوعية: coagulation, platelet aggregation, equine, first long-term training, Thoroughbred racehorse, Veterinary medicine, SF600-1100, Zoology, QL1-991

الوصف: Training has a significant effect on the physiology of blood coagulation in humans and in horses. Several hemostatic changes have been reported after exercise in the horse but data available are inconclusive. The aim of this study was to investigate platelet activation and primary platelet-related hemostasis modifications in young never-trained Thoroughbreds in the first incremental training period in order to improve knowledge on this topic. Twenty-nine clinically healthy, untrained, 2-year-old Thoroughbred racehorses were followed during their incremental 4-month sprint exercise training. Blood collection was performed once a month, five times in total (T-30, T0, T30, T60, and T90). Platelet aggregation was measured by light transmission aggregometry in response to various agonists: adenosine diphosphate (ADP), collagen, and calcium ionophore A23187. Platelet function was evaluated using a platelet function analyzer (PFA-100®) using collagen/ADP and collagen/adrenaline cartridges. Nitrite-nitrate (NOx) plasma concentrations were measured via a colorimetric assay to assess in vivo nitric oxide bioavailability. Platelet activation was also investigated through gene expression analyses (selectin P-SELP, ectonucleotidase CD39-ENTPD1, prostaglandin I2 synthase-PTGIS, endothelial nitric oxide synthase 3-NOS3). Differences among the time points were analyzed and mean ± SEM were calculated. Significant modifications were identified compared with T-30, with an increase in platelet aggregation (collagen:32.6 ± 4.8 vs. 21.6 ± 4.9%; ADP: 35.5 ± 2.0 vs. 24.5 ± 3.1%; A23187: 30 ± 4.7 vs. 23.8 ± 4%) and a shorter closure time of C-ADP cartridges (75.6 ± 4.4 vs. 87.7 ± 3.4 s) that tended to return to the baseline value at T90. NOx concentrations in plasma significantly increased after 30 days of the training program compared with the baseline. The first long-term training period seems to induce platelet hyperactivity after 30 days in never-trained Thoroughbreds. Regular physical training reduces the negative effects of acute efforts on platelet activation.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2076-2615/14/3/414Test; https://doaj.org/toc/2076-2615Test

الوصول الحر: https://doaj.org/article/4cde78ae9c344de9ae38a645d7ff5bfcTest

المؤلفون: Lena Tschiderer, Lisa Seekircher, Raffaele Izzo, Costantino Mancusi, Maria V. Manzi, Damiano Baldassarre, Mauro Amato, Elena Tremoli, Fabrizio Veglia, Tomi‐Pekka Tuomainen, Jussi Kauhanen, Ari Voutilainen, Bernhard Iglseder, Lars Lind, Tatjana Rundek, Moise Desvarieux, Akihiko Kato, Eric de Groot, Gülay Aşçi, Ercan Ok, Stefan Agewall, Joline W. J. Beulens, Christopher D. Byrne, Philip C. Calder, Hertzel C. Gerstein, Paolo Gresele, Gerhard Klingenschmid, Michiaki Nagai, Michael H. Olsen, Grace Parraga, Maya S. Safarova, Naveed Sattar, Michael Skilton, Coen D. A. Stehouwer, Heiko Uthoff, Michiel A. van Agtmael, Amber A. van der Heijden, Dorota A. Zozulińska‐Ziółkiewicz, Hyun‐Woong Park, Moo‐Sik Lee, Jang‐Ho Bae, Oscar Beloqui, Manuel F. Landecho, Matthieu Plichart, Pierre Ducimetiere, Jean Philippe Empana, Lena Bokemark, Göran Bergström, Caroline Schmidt, Samuela Castelnuovo, Laura Calabresi, Giuseppe D. Norata, Liliana Grigore, Alberico Catapano, Dong Zhao, Miao Wang, Jing Liu, M. Arfan Ikram, Maryam Kavousi, Michiel L. Bots, Michael J. Sweeting, Matthias W. Lorenz, Peter Willeit

المصدر: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, Vol 12, Iss 12 (2023)

مصطلحات موضوعية: carotid intima‐media thickness, carotid plaque, individual participant data meta‐analysis, prospective studies, Diseases of the circulatory (Cardiovascular) system, RC666-701

الوصف: Background The association between common carotid artery intima‐media thickness (CCA‐IMT) and incident carotid plaque has not been characterized fully. We therefore aimed to precisely quantify the relationship between CCA‐IMT and carotid plaque development. Methods and Results We undertook an individual participant data meta‐analysis of 20 prospective studies from the Proof‐ATHERO (Prospective Studies of Atherosclerosis) consortium that recorded baseline CCA‐IMT and incident carotid plaque involving 21 494 individuals without a history of cardiovascular disease and without preexisting carotid plaque at baseline. Mean baseline age was 56 years (SD, 9 years), 55% were women, and mean baseline CCA‐IMT was 0.71 mm (SD, 0.17 mm). Over a median follow‐up of 5.9 years (5th–95th percentile, 1.9–19.0 years), 8278 individuals developed first‐ever carotid plaque. We combined study‐specific odds ratios (ORs) for incident carotid plaque using random‐effects meta‐analysis. Baseline CCA‐IMT was approximately log‐linearly associated with the odds of developing carotid plaque. The age‐, sex‐, and trial arm–adjusted OR for carotid plaque per SD higher baseline CCA‐IMT was 1.40 (95% CI, 1.31–1.50; I2=63.9%). The corresponding OR that was further adjusted for ethnicity, smoking, diabetes, body mass index, systolic blood pressure, low‐ and high‐density lipoprotein cholesterol, and lipid‐lowering and antihypertensive medication was 1.34 (95% CI, 1.24–1.45; I2=59.4%; 14 studies; 16 297 participants; 6381 incident plaques). We observed no significant effect modification across clinically relevant subgroups. Sensitivity analysis restricted to studies defining plaque as focal thickening yielded a comparable OR (1.38 [95% CI, 1.29–1.47]; I2=57.1%; 14 studies; 17 352 participants; 6991 incident plaques). Conclusions Our large‐scale individual participant data meta‐analysis demonstrated that CCA‐IMT is associated with the long‐term risk of developing first‐ever carotid plaque, independent of traditional cardiovascular risk factors.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2047-9980Test

الوصول الحر: https://doaj.org/article/23529eb502664afe812ac7bfbd51a9b2Test

المؤلفون: Giacomo Cafaro, Eleonora Petito, Onelia Bistoni, Emanuela Falcinelli, Sabrina Cipriani, Maria Chiara Borghi, Angelo F. Bonifacio, Elisa Giglio, Alessia Alunno, Carlo Perricone, Roberto Gerli, Paolo Gresele, Elena Bartoloni

المصدر: Arthritis Research & Therapy, Vol 24, Iss 1, Pp 1-9 (2022)

مصطلحات موضوعية: Methotrexate, Rheumatoid arthritis, Endothelial dysfunction, Cardiovascular disease, Angiogenic T cells, Platelets, Diseases of the musculoskeletal system, RC925-935

الوصف: Abstract Background Endothelial dysfunction contributes to increased cardiovascular (CV) disease in rheumatoid arthritis (RA). Angiogenic T cells (Tang) are a key regulator of vascular function via their interaction with endothelial progenitor cells (EPCs). Methotrexate (MTX) has been associated to reduced CV disease risk, but its effects on endothelial homeostasis have been poorly explored. We investigated MTX effects on endothelial homeostasis in early, treatment-naïve RA patients. Methods Fifteen untreated, early RA patients and matched healthy controls (HC) were enrolled. RA patients with long-standing disease in remission or low disease activity treated with MTX for at least 6 months were selected as controls. Circulating CD28+ and CD28null Tang cell, endothelial microparticle (EMP), EPC and soluble vascular cell adhesion molecule (sVCAM)-1 levels were measured. Results Tang percentage was higher in early RA than in HCs and significantly increased after 3-month MTX treatment. Tang cells in RA were characterized by higher percentage of CD28null and lower CD28-positive cells than HCs. MTX restored a Tang cell phenotype similar to HCs. Altered sVCAM-1, EMP and EPC were restored to levels similar to HCs after a 3-month MTX. Biomarker levels after 3 months of MTX were not different to those of patients with long-standing treatment. Conclusions MTX has a positive effect on Tang, sVCAM-1, EPCs and EMPs in RA. Restoration of imbalance between CD28 + and CD28null Tang by MTX may be one of the mechanisms underlying its favourable effects on endothelial dysfunction. These effects seem to be long-lasting and independent from systemic inflammation reduction, suggesting a direct effect of MTX on the endothelium.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1478-6362Test

الوصول الحر: https://doaj.org/article/3691282f13084121beb992f2bbebd118Test

المؤلفون: Stefania Momi, Paolo Gresele

المصدر: Bleeding, Thrombosis and Vascular Biology, Vol 2, Iss 1 (2023)

مصطلحات موضوعية: platelets, phylogeny, Diseases of the circulatory (Cardiovascular) system, RC666-701

الوصف: Blood platelets are indubitably one of the wonders of the world, whether the first of the eight may be a matter of debate, but they certainly are. Compared with all other cells they are much smaller and, like few others, devoid of a nucleus but despite this, they are extremely complex and provided with multiple functions [...].

وصف الملف: electronic resource

العلاقة: https://btvb.org/btvb/article/view/63Test; https://doaj.org/toc/2785-5309Test

الوصول الحر: https://doaj.org/article/4b69b3f4b29641a7a07cdb7f4d4a0d69Test

المؤلفون: Rosa Curcio, Vito Gandolfo, Riccardo Alcidi, Luciano Giacomino, Tommaso Campanella, Genni Casarola, Rachele Rossi, Lorenzo Chiatti, Marco D'Abbondanza, Rita Commissari, Paolo Gresele, Giacomo Pucci, Gaetano Vaudo

المصدر: International Journal of Infectious Diseases, Vol 116, Iss , Pp 154-156 (2022)

مصطلحات موضوعية: COVID-19, Janssen, AD26.COV2.S, Vaccination, Vaccine-induced thrombosis with thrombocytopenia, Pulmonary embolism, Infectious and parasitic diseases, RC109-216

الوصف: ABSTRACT: Vaccine-induced immune thrombotic thrombocytopenia (VITT) has emerged as a rare side effect of adenoviral vector-based vaccines against coronavirus disease 2019 (COVID-19), and is most frequently reported after use of the Vaxzevria (AstraZeneca) vaccine. This report describes a case of severe thrombocytopenia associated with massive pulmonary embolism and portal vein thrombosis occurring 13 days after the administration of the single-dose adenoviral vector-based vaccine Ad26.COV2.S (Janssen Vaccines). Based on early clinical suspicion, the patient quickly received treatment with corticosteroids and intravenous immunoglobulin, followed by a rapid increase in platelet count that allowed timely administration of full-dose anticoagulation. Treatment with intravenous immunoglobulin, however, could mask the ability of anti-platelet factor 4-heparin antibodies to bind and activate platelets in the presence of heparin, leading to false-negative results on the immunoassay functional test. Therefore, if VITT is suspected, blood samples for diagnostic confirmation should be collected prior to any treatment to improve diagnostic performance.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S1201971221012443Test; https://doaj.org/toc/1201-9712Test

الوصول الحر: https://doaj.org/article/fad070e4e8814f13a6a2ed8ff7c7aa88Test