المؤلفون: Kaivalya Gongadashetti, Pankush Gupta, Rima Dada, Neena Malhotra

المصدر: International Journal of Reproductive BioMedicine, Vol 19, Iss 5, Pp 449-456 (2021)

مصطلحات موضوعية: oxidative stress, art, pcos, infertility, 8-ip., Gynecology and obstetrics, RG1-991, Reproduction, QH471-489

الوصف: Background: Polycystic ovarian syndrome (PCOS) is the most common cause of anovulatory infertility. Oxidative stress (OS), which plays an important role in determining the developmental competence of an oocyte, may be involved in understanding infertility and poor outcomes cycles in PCOS women undergoing in vitro fertilization (IVF). Objective: To measure OS biomarkers in the follicular fluid of PCOS women undergoing IVF. Materials and Methods: In this cross-sectional study, 100 women with PCOS (n = 43) and tubal factor (n = 57) undergoing IVF, who were referred to a tertiary medical center between January 2016 and September 2017 were enrolled. OS markers like reactive oxygen species (ROS), total antioxidant capacity (TAC), and 8-Isoprostane (8-IP) were tested in the follicular fluid and various IVF outcomes in the form of oocytes retrieved, fertilized, cleavage rate, grading of embryos and pregnancy outcomes were compared between the two groups. Results: The results indicated that the levels of ROS, TAC, and 8-IP were higher in the PCOS group compared to the tubal group (p = 0.21, p = 0.95, and p < 0.05, respectively). Biomarkers based on the number of retrieved oocytes, cleavage rate, and grades of embryos did not differ significantly in the two groups. The median ROS, TAC, and 8-IP were not significantly different in the two groups in relation to the pregnancies, although the 8-IP levels were significantly raised in the PCOS women who had a miscarriage (p = 0.02). Conclusion: This study concluded the possible role of OS in PCOS women with increased higher level of 8-IP biomarker as a potential biomarker that needs further evaluation.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2476-4108Test; https://doaj.org/toc/2476-3772Test

الوصول الحر: https://doaj.org/article/02b25bc5569f4b8f83e07ae8cd459891Test

المؤلفون: Pankush Gupta, Aashish Choudhary, Giridara Gopal, Rajeev Kumar, Arbind Kumar, Pawan Tiwari, Neena Malhotra

المصدر: Indian Journal of Urology, Vol 37, Iss 4, Pp 331-334 (2021)

مصطلحات موضوعية: Diseases of the genitourinary system. Urology, RC870-923

الوصف: Introduction: SARS-CoV-2 has been detected in various body fluids. Its presence in semen has been tested with contradictory results. This study aimed to detect the presence of SARS-CoV-2 virus using the real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) in semen and seminal plasma from men with active COVID-19 infection. Methods: In a cross-sectional study at a COVID facility, men aged 20–45 years with active COVID-19 infection provided semen samples within 7 days of symptom onset or 5 days of nasopharyngeal rRT-PCR test positivity in asymptomatic men. Testing of SARS-CoV-2 was performed using rRT-PCR and semen analysis was done for sperm counts and motility as per the WHO (2010) standards. Results: A total of 37 men with a mean age of 32.2 ± 5.6 years were tested. SARS CoV-2 virus could not be isolated in any of the samples. Further, microscopic analysis done on 17 samples showed normal semen parameters during the active phase of disease. Conclusion: Men with mild COVID-19 disease or asymptomatic individuals do not shed virus in their semen, ruling out sexual contact as a mode of transmission in this subset of population.

وصف الملف: electronic resource

العلاقة: http://www.indianjurol.com/article.asp?issn=0970-1591;year=2021;volume=37;issue=4;spage=331;epage=334;aulastTest=Gupta; https://doaj.org/toc/0970-1591Test; https://doaj.org/toc/1998-3824Test

الوصول الحر: https://doaj.org/article/e74f03e112304262af2d770685db2fd3Test

المؤلفون: Ritesh Goel, Pankush Gupta

المصدر: Indian Journal of Urology, Vol 38, Iss 4, Pp 327-327 (2022)

مصطلحات موضوعية: Diseases of the genitourinary system. Urology, RC870-923

وصف الملف: electronic resource

العلاقة: http://www.indianjurol.com/article.asp?issn=0970-1591;year=2022;volume=38;issue=4;spage=327;epage=327;aulast=GoelTest; https://doaj.org/toc/0970-1591Test; https://doaj.org/toc/1998-3824Test

الوصول الحر: https://doaj.org/article/61e941db77864d2a991bb6e7ea703684Test

المؤلفون: Neena Malhotra, Nomita Chandhiok, Venkateswaran K. Iyer, Urvashi B. Singh, Anil Sharma, Aijaz Ahmad, Pankush Gupta

المصدر: Indian Journal of Tuberculosis. 69:465-469

مصطلحات موضوعية: Infertility, medicine.medical_specialty, Tuberculosis, Biopsy, Endometrium, Gastroenterology, Interferon-gamma, Pregnancy, Internal medicine, medicine, Humans, Interferon gamma, Prospective Studies, Prospective cohort study, medicine.diagnostic_test, business.industry, medicine.disease, Tuberculosis, Female Genital, Infectious Diseases, medicine.anatomical_structure, Granuloma, Female, business, Biomarkers, Endometrial biopsy, medicine.drug

الوصف: Background Female genital tuberculosis (FGTB) causes infertility in a significant number of females. The immunological impact of tuberculosis on endometrium in infertile females has not been studied before. The present study was designed to evaluate markers related to infiltrating immune cells and implantation in endometrial aspiration from infertile females and correlate with conventional tests and polymerase chain reaction (PCR) for tuberculosis (TB). Methods It was a prospective cohort study with 385 patients out of which IHC was done in 306 over a period of 3 years from 2013 to 2016 in a tertiary care hospital. Women with infertility, 20–35 years of age, without history of pulmonary TB or intake of antitubercular therapy were included. Endometrial samples were subjected to PCR for TB along with microbiological and histological examination for TB. Immunohistochemistry for CD45, CD3, CD20, CD4, CD8, CD68, CD138, Interferon gamma, Interleukin 10 (IL-10) and implantation markers MUC1 and Notch 1 were done on the endometrial samples along with 25 control subjects. Results Conventional tests for tuberculosis like staining for acid fast bacilli (AFB), granuloma on histology or culture positivity were seen in 2.61% (6/306; 1.96% had granulomas, 1/306; 0.32% was AFB positive, 2/306; 0.6% were liquid culture positive). PCR was positive in 190/306 (62.09%). CD3, CD20, CD45, CD68, CD4, CD8 and CD 138 expressing infiltrating cells were not significantly related to PCR positive cases. Interferon gamma expressing lymphocytes were significantly higher (38.94%) in PCR positive endometria compared to 26.72% in the PCR negative (p = 0.04). Notch −1 expression correlated significantly with the occurrence of pregnancy. A trend towards high intensity expression of Notch1 was seen in PCR negative cases. MUC-1 expression did not correlate with pregnancy although interferon gamma expression was significantly related to low intensity MUC1 expression. Conclusions Immunohistochemical markers are not reliable tests in diagnosis of FGTB. Notch 1 expression though showing correlation with pregnancy has to be further evaluated with a panel of other implantation markers. Study funding Indian Council of Medical Research, New Delhi, India.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7056e497552c9e38f3623a1192766b4cTest
https://doi.org/10.1016/j.ijtb.2021.07.008Test

المؤلفون: Neena Malhotra, Pankush Gupta, Rima Dada, Kaivalya Gongadashetti

المصدر: International Journal of Reproductive BioMedicine, Vol 19, Iss 5, Pp 449-456 (2021)
International Journal of Reproductive Biomedicine

مصطلحات موضوعية: Infertility, QH471-489, Cross-sectional study, medicine.medical_treatment, medicine.disease_cause, Miscarriage, Anovulation, Andrology, medicine, PCOS, In vitro fertilisation, business.industry, Reproduction, Obstetrics and Gynecology, Gynecology and obstetrics, medicine.disease, Follicular fluid, female genital diseases and pregnancy complications, oxidative stress, art, pcos, infertility, 8-ip, 8-IP, Reproductive Medicine, Oxidative stress, RG1-991, Biomarker (medicine), Original Article, business, ART

الوصف: Background: Polycystic ovarian syndrome (PCOS) is the most common cause of anovulatory infertility. Oxidative stress (OS), which plays an important role in determining the developmental competence of an oocyte, may be involved in understanding infertility and poor outcomes cycles in PCOS women undergoing in vitro fertilization (IVF). Objective: To measure OS biomarkers in the follicular fluid of PCOS women undergoing IVF. Materials and Methods: In this cross-sectional study, 100 women with PCOS (n = 43) and tubal factor (n = 57) undergoing IVF, who were referred to a tertiary medical center between January 2016 and September 2017 were enrolled. OS markers like reactive oxygen species (ROS), total antioxidant capacity (TAC), and 8-Isoprostane (8-IP) were tested in the follicular fluid and various IVF outcomes in the form of oocytes retrieved, fertilized, cleavage rate, grading of embryos and pregnancy outcomes were compared between the two groups. Results: The results indicated that the levels of ROS, TAC, and 8-IP were higher in the PCOS group compared to the tubal group (p = 0.21, p = 0.95, and p < 0.05, respectively). Biomarkers based on the number of retrieved oocytes, cleavage rate, and grades of embryos did not differ significantly in the two groups. The median ROS, TAC, and 8-IP were not significantly different in the two groups in relation to the pregnancies, although the 8-IP levels were significantly raised in the PCOS women who had a miscarriage (p = 0.02). Conclusion: This study concluded the possible role of OS in PCOS women with increased higher level of 8-IP biomarker as a potential biomarker that needs further evaluation. Key words: Oxidative stress, ART, PCOS, Infertility, 8-IP.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14cd35f46610f059218a6faadd7aa95eTest
https://doaj.org/article/02b25bc5569f4b8f83e07ae8cd459891Test

المؤلفون: Pankush Gupta, Neena Malhotra, Anshu Yadav, Monika Saini, Chithira Sridhar

المصدر: JBRA Assisted Reproduction

مصطلحات موضوعية: Pediatrics, medicine.medical_specialty, medicine.diagnostic_test, business.industry, Ovarian hyperstimulation syndrome, Late onset, Case Report, late onset OHSS, medicine.disease, Embryo transfer, hemorrhagic ascites, Transaminitis, Etiology, Paracentesis, medicine, transaminitis, Gestation, business, Complication

الوصف: Ovarian hyperstimulation syndrome (OHSS) is an entity associated with controlled ovarian stimulation (COS) in assisted reproduction. It presents a great challenge for clinicians concerning a timely diagnosis and intervention, because it is a life-threatening condition. We came across a rare case of late onset severe OHSS, which lasted till 19 weeks of gestation (POG) with diagnostic challenge in which the patient had hemorrhagic ascites post embryo transfer, and transaminitis. The case was managed effectively with repeated paracentesis along with replacement therapy, and the transaminitis was also gradually resolved. Severe OHSS may follow an unusually prolonged course associated with transaminitis. Our case suggests that it is crucial to distinguish between the nature and etiology of transaminitis along with OHSS, and other pathologies like drug induced transaminitis should also be kept in mind.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e93eb02586dcd55b5ecc008f13b2e28Test
http://europepmc.org/articles/PMC8769176Test

المؤلفون: Pankush Gupta, Aashish Choudhary, Deepankar Srigyan, Neena Malhotra

المصدر: JBRA assisted reproduction.

مصطلحات موضوعية: Obstetrics and Gynecology

الوصف: To detect the shedding of SARS-CoV-2 in cervicovaginal secretions of women with active COVID 19 infection.A cross-sectional study from a COVID facility including women aged 20-45 years with active COVID-19 infection, cervicovaginal secretions were collected from cervix and posterior fornix using dacron swab within 7 days of symptom onset or 5 days of nasopharyngeal rRT-PCR test positivity in asymptomatic women. Cervicovaginal samples of women with mild symptoms were tested using rRT-PCR for SARS-CoV-2.SARS-CoV-2 was not detected in cervicovaginal secretions of any of the 11 women included in the study.SARS-CoV-2 does not shed in the cervicovaginal secretions of women with mild COVID 19 infection, ruling out sexual and vertical transmission of virus in mild and asymptomatic disease.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad2542099d9b972c8c4c20cd9b4ffeadTest
https://pubmed.ncbi.nlm.nih.gov/34995047Test

المؤلفون: Neena Malhotra, Chithira P.V. SR, Pankush Gupta

المصدر: Fertility and Sterility. 118:e26

مصطلحات موضوعية: Reproductive Medicine, Obstetrics and Gynecology

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::ec5dec31d660fa854cbc6f7d25054909Test
https://doi.org/10.1016/j.fertnstert.2022.08.093Test

المؤلفون: Anupama Hari, Amita Jain, Neena Malhotra, Venkateswaran K. Iyer, S. P. Jaiswar, Lakhbir Kaur Dhaliwal, Urvashi B. Singh, Nomita Chandhiok, Aparna Srikantam, Aijaz Ahmad, Pankush Gupta, Shalini Gainder

المصدر: European journal of obstetrics, gynecology, and reproductive biology. 267

مصطلحات موضوعية: Pathology, medicine.medical_specialty, Tuberculosis, Biopsy, Antitubercular Agents, Endometrium, Immune system, medicine, Humans, Subclinical infection, CD20, medicine.diagnostic_test, biology, business.industry, Obstetrics and Gynecology, Mycobacterium tuberculosis, medicine.disease, Tuberculosis, Female Genital, medicine.anatomical_structure, Reproductive Medicine, Granuloma, biology.protein, Immunohistochemistry, Female, business, Infertility, Female, Endometrial biopsy

الوصف: Background Female Genital Tuberculosis (FGTB) causes infertility in a large number of females in developing countries. Presence of granuloma on histopathological examination of endometrial samples is diagnostic of FGTB. But immunohistochemical evaluation of endometrial aspirates has not been explored before. Aim To evaluate the immunohistochemical delineation of immune cells in FGTB. Methods 1515 infertile women from 20 to 35 years were enrolled and underwent endometrial aspiration (EA), which was subjected to microbiological and histopathological examination along with PCR. Patients positive for conventional tests like granulomas, acid fast bacilli, mycobacterial culture on LJ medium or liquid (MGIT) culture were started on antitubercular therapy. Conventional test negative but PCR positive patients were posted for laparoscopy. Immunohistochemistry (IHC) for LCA, CD68, CD3, CD4, CD8, CD 20, CD138, IFN gamma and IL10 were evaluated. Result 38/1515 (2.5%) subjects tested positive for conventional methods. PCR-TB was positive in 615/1515 samples (40.59%). On IHC, the number of CD45 (LCA) positive immune cells (p = 0.03) and IFN gamma (p = 0.002) and IL10 expression (p = 0.012) at 1 + level were higher in the PCR positive samples. Laparoscopy done in 418/463 patients and 89/418 (21.3%) showed definitive findings of tuberculosis. CD3, CD4, CD8, CD20, CD68 and CD138 showed no correlation with PCR and laparoscopy. Conclusion Increased IFN gamma and IL 10 expressing immune cells in PCR positive EA suggests subclinical early changes, and can be useful as a research tool but have no role in diagnosing FGTB.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4638c8ccf485a356819e3dce353437dbTest
https://pubmed.ncbi.nlm.nih.gov/34800826Test

المؤلفون: Arbind Kumar, Aashish Choudhary, Pankush Gupta, Giridara Gopal, Pawan Tiwari, Neena Malhotra, Rajeev Kumar

المصدر: Indian Journal of Urology : IJU : Journal of the Urological Society of India
Indian Journal of Urology, Vol 37, Iss 4, Pp 331-334 (2021)

مصطلحات موضوعية: education.field_of_study, medicine.diagnostic_test, business.industry, Transmission (medicine), Urology, Population, Physiology, Semen, Semen analysis, urologic and male genital diseases, Asymptomatic, Virus, Reverse transcriptase, Diseases of the genitourinary system. Urology, law.invention, law, medicine, Original Article, RC870-923, medicine.symptom, business, education, Polymerase chain reaction

الوصف: Introduction: SARS-CoV-2 has been detected in various body fluids. Its presence in semen has been tested with contradictory results. This study aimed to detect the presence of SARS-CoV-2 virus using the real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) in semen and seminal plasma from men with active COVID-19 infection. Methods: In a cross-sectional study at a COVID facility, men aged 20–45 years with active COVID-19 infection provided semen samples within 7 days of symptom onset or 5 days of nasopharyngeal rRT-PCR test positivity in asymptomatic men. Testing of SARS-CoV-2 was performed using rRT-PCR and semen analysis was done for sperm counts and motility as per the WHO (2010) standards. Results: A total of 37 men with a mean age of 32.2 ± 5.6 years were tested. SARS CoV-2 virus could not be isolated in any of the samples. Further, microscopic analysis done on 17 samples showed normal semen parameters during the active phase of disease. Conclusion: Men with mild COVID-19 disease or asymptomatic individuals do not shed virus in their semen, ruling out sexual contact as a mode of transmission in this subset of population.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0e1e23e84c61e5be734bb84b6e24c07Test
https://pubmed.ncbi.nlm.nih.gov/34759524Test