المؤلفون: Catharina Gerhards, Marlene Steingass, Alexandra Heininger, Bettina Lange, Michael Hetjens, Marlis Gerigk, Michael Neumaier, Osman Evliyaoglu, Maximilian Kittel

المصدر: Vaccines, Vol 12, Iss 2, p 163 (2024)

مصطلحات موضوعية: anti-SARS-CoV-2 antibodies, anti-N abs, anti-S1/RBS, SARS-CoV-2 vaccination, COVID-19 after vaccination, vaccination breakthrough, Medicine

الوصف: Background: The aim of the rapid introduction of vaccines during the COVID-19 pandemic was a reduction in SARS-CoV-2 transmission and a less frequent occurrence of severe COVID-19 courses. Thus, we evaluated COVID-19 severity in vaccinated individuals to examine variant-specific symptom characteristics and their clinical impact on the serological immune response. Methods: A total of 185 individuals previously vaccinated against and infected with the SARS-CoV-2 Delta (B.1.617.2) or Omicron (BA.4 and BA.5) variant, were enrolled for anti-SARS-CoV-2 anti-N- and anti-RBD/S1-Ig level detection. A structured survey regarding medical history was conducted. Results: In 99.5 percent of cases, outpatient treatment was satisfactory. Specific symptoms associated with variants included ageusia and anosmia in patients with Delta infections and throat pain in Omicron infections. Among Delta-infected individuals with specific symptoms, significantly higher levels of anti-N antibodies were observed. Conclusion: Our study identified variant-specific differences in the amount of SARS-CoV-2 antibody production and COVID-19 symptoms. Despite this, vaccinated individuals with Omicron or Delta infections generally experienced mild disease courses. Additionally, asymptomatic individuals exhibit lower anti-SARS-CoV-2 antibody levels, indicating a clinical correlation between disease-specific antibodies and distinct symptoms, particularly in the case of the Delta variant. In follow-up studies, exploring post-COVID syndrome and focusing on cognitive symptoms in the acute phase of Omicron infections is crucial as it has the potential to longitudinally impact the lives of those affected.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2076-393X/12/2/163Test; https://doaj.org/toc/2076-393XTest

الوصول الحر: https://doaj.org/article/02b70f94995d457581db48ddd77d982aTest

المؤلفون: Wolfgang Stremmel, Hüseyin Vural, Osman Evliyaoglu, Ralf Weiskirchen

المصدر: MMW - Fortschritte der Medizin. 164:3-11

مصطلحات موضوعية: General Medicine

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::5ddd9def06174fafbcecd9c82a8f71b4Test
https://doi.org/10.1007/s15006-022-0832-0Test

المؤلفون: Wolfgang, Stremmel, Hüseyin, Vural, Osman, Evliyaoglu, Ralf, Weiskirchen

المصدر: MMW Fortschritte der MedizinLiteratur. 164(Suppl 7)

مصطلحات موضوعية: Lecithins, Remission Induction, Quality of Life, Humans, Colitis, Ulcerative

الوصف: Phosphatidylcholine is an essential component of the intestinal mucus and serves as a protective shield against the ingress of bacteria from the stool. In the intestinal mucus of patients with ulcerative colitis, phosphatidylcholine is reduced by 70%, which makes the intestine susceptible to bacterial inflammation. Local application by administering enteric phosphatidylcholine could compensate for this deficiency.A summary analysis of three clinical studies published until now with 160 included patients with ulcerative colitis was performed.The meta-analysis showed that lecithin enriched with phosphatidylcholine and microencapsulated with Eudragit S-100 significantly improved the remission rate as well as the clinical and endoscopic picture. There was also an improvement in histology and quality of life. All parameters were significantly superior to placebo. The remission achieved was maintained significantly longer with enteric lecithin than with placebo. The side effect profile was identical to the placebo group, which is particularly important for the patients. In complementary medicine, phosphatidylcholine can be seen as protection for the intestines.Hintergrund: Phosphatidylcholin ist ein essenzieller Bestandteil des Darmschleims und dient als Schutzschild gegen das Eindringen von Bakterien aus dem Stuhl. Im Darmschleim von Patienten mit Colitis ulcerosa ist Phosphatidylcholin um 70% vermindert, welches den Darm anfällig gegenüber bakteriell ausgelösten Entzündungen macht. Lokale Applikation durch Gabe von darmlöslichem Phosphatidylcholin könnte diesen Mangel ausgleichen.Es wurde eine zusammenfassende Analyse der drei bisher veröffentlichten klinischen Studien mit 160 eingeschlossenen Colitis-ulcerosa-Patienten durchgeführt.Ergebnisse und Schlussfolgerung: Die Metaanalyse zeigte, dass mit Phosphatidylcholin angereichertes und mit Eudragit S-100 mikroverkapseltes Lecithin die Remissionsrate sowie das klinische und endoskopische Bild deutlich verbessert. Auch in Bezug auf Histologie und Lebensqualität war eine Besserung festzustellen. Gegenüber Placebo waren alle Parameter signifikant überlegen. Die erreichte Remission blieb unter darmlöslichem Lecithin deutlich länger erhalten als unter Placebo. Das Nebenwirkungsprofil war identisch zur Placebogruppe, was für die Patienten besonders wichtig ist. Phosphatidylcholin ist damit komplementärmedizinisch als Schutz für den Darm anzusehen.Eingereicht am 22.10.2021 - Revision akzeptiert am 16.12.2021.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::87c4a84658a07c389ea9685717f05367Test
https://pubmed.ncbi.nlm.nih.gov/35831742Test

المؤلفون: Eren Vurgun, Osman Evliyaoğlu, Çiğdem Arabacı, Sembol Yıldırmak, Pınar Çakmak

المصدر: İstanbul Medical Journal, Vol 18, Iss 2, Pp 72-75 (2017)

مصطلحات موضوعية: Procalcitonin, C-reactive protein, sepsis, gram-positive bacteria, gram-negative bacteria, Medicine

الوصف: Objective: The primary aim of our study was to investigate the usefulness of serum C-reactive protein (CRP) and procalcitonin (PCT) levels in the differential diagnosis of causative gram-positive (Gram+) or gram-negative (Gram-) bacteria in patients with sepsis to facilitate decisions concerning the initial choice of an empiric antibiotic regimen.Methods: Between February 2014 and February 2016, 47 patients who had sepsis diagnosed on the basis of positive blood cultures and clinical examination results were retrospectively enrolled. Serum CRP and PCT levels of 15 gram+ and 32 gram- bacterial sepsis groups were compared using the Mann–Whitney U test. The correlation between CRP and PCT levels was calculated using the Spearman’s test.Results: Among patients with bacterial sepsis, the median CRP level was 91.42 mg/L and the median PCT level was 0.46 ng/mL. There were no significant difference in CRP and PCT levels between the gram+ and gram- sepsis groups (p=0.98 and p=0.21, respectively). There was good correlation between the CRP and PCT levels (r=0.64, p<0.001).Conclusion: Considering the changes and the status of proinflammatory/ anti-inflammatory responses in the pathogenesis of sepsis, we believe that CRP and PCT levels alone are insufficient for predicting the type of causative bacteria in sepsis.

العلاقة: http://imj.galenos.com.tr/archives/archive-detail/article-preview/roles-of-c-reactive-protein-and-procalcitonin-in-e/20452Test; https://doaj.org/toc/2619-9793Test; https://doaj.org/toc/2148-094XTest; https://doaj.org/article/735a92f574c94a2f8f7566023a121701Test

الإتاحة: https://doi.org/10.5152/imj.2017.27132Test
https://doaj.org/article/735a92f574c94a2f8f7566023a121701Test

المؤلفون: Wolfgang Stremmel, Ralf Weiskirchen, Huseyin Vural, Osman Evliyaoglu

المصدر: Digestive Diseases. 39:508-515

مصطلحات موضوعية: medicine.medical_specialty, education.field_of_study, business.industry, Population, Gastroenterology, General Medicine, Odds ratio, Placebo, medicine.disease, Ulcerative colitis, law.invention, Clinical trial, Randomized controlled trial, law, Internal medicine, Meta-analysis, medicine, Adverse effect, education, business

الوصف: Background: Phosphatidylcholine (PC) is intrinsically missing in intestinal mucus of patients with ulcerative colitis. Topical supplementation with delayed intestinal release PC formulations is assumed to compensate this lack. Three monocenter randomized controlled trials (RCTs) with a 30% PC-containing lecithin were successful, whereas 1 trial with >94% PC-containing lecithin failed. Objectives: Evaluation of 30% PC-containing lecithin provided in a delayed intestinal release formulation for treatment efficacy of ulcerative colitis was evaluated by meta-analysis of 3 RCTs. Methods: Meta-analysis of 3 studies was performed using RevMan 5.3 software. Odds ratio (OR) and 95% Cl were calculated for remission, clinical and endoscopic improvement, histology, and life quality. p values Results: The meta-analysis of 3 RTCs with 160 included patients with ulcerative colitis verified that PC improved the rate of remission (OR = 9.68), as well as clinical (OR = 30.58) and endoscopic outcomes (OR = 36.73). Within the available patient population, also histology and quality of life became better. All effects were significant over placebo. Achieved remission was maintained in a higher percentage of patients under intestinal-release PC formulation than placebo. The profile of adverse events was identical to the placebo population. Conclusions: A 30% PC-containing lecithin in delayed intestinal release formulation improves clinical and endoscopic outcomes, histologic activity, and quality of life in patients with ulcerative colitis. For the patients, lack of adverse events is an important consideration.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::b07345753b20749ce3045ad539227c9fTest
https://doi.org/10.1159/000514355Test

المؤلفون: Eren Vurgun, Osman Evliyaoğlu, Sembol Yıldırmak

المصدر: Haseki Tıp Bülteni, Vol 54, Iss 2, Pp 76-82 (2016)

مصطلحات موضوعية: Vitamin D deficiency, parathyroid hormone, calcium, Medicine, Medicine (General), R5-920

الوصف: Aim: There is lack of studies on the relationship between parathyroid hormone and vitamin D levels for defining deficiency and insufficiency of vitamin D in adults living in Turkey. Therefore, we searched for threshold value of 25-hydroxyvitamin D concentration below which parathyroid hormone levels would significantly increase. Methods: Between the dates of 01.03.2015 and 01.06.2015, the results of patients older than 18 years were scanned from the laboratory information system. Eight hundred three patients, whose simultaneously analyzed serum levels of calcium, inorganic phosphate, creatinine and intact parathyroid hormone were within the reference ranges and 25-hydroxyvitamin D was <50 ng/mL, were included in the study. Results: There was a significant difference in intact parathyroid hormone levels between subjects with 25-hydroxyvitamin D levels of 15-10 and those with 10-5 ng/mL (p=0.05). A significant increase in intact parathyroid hormone levels (p=0.01) was found to start with a 25-hydroxyvitamin D level below 22 ng/mL within the range of 30 to 10 ng/mL. Consequently, we determined the first 25-hydroxyvitamin D threshold in which intact parathyroid hormone levels significantly increase to be 22 ng/mL and the second threshold as 10 ng/mL. Conclusion: Evaluation of parathyroid hormone which has a major role in the regulation of vitamin D should be done to define and diagnose vitamin D deficiency and insufficiency correctly.

العلاقة: http://www.hasekidergisi.com/article_10721/Evidence-based-Laboratory-Determining-The-Insufficiency-Level-Of-Vitamin-DTest; https://doaj.org/toc/1302-0072Test; https://doaj.org/toc/2147-2688Test; https://doaj.org/article/c56263e109574caba90aa4f5471e53feTest

الإتاحة: https://doi.org/10.4274/haseki.2854Test
https://doaj.org/article/c56263e109574caba90aa4f5471e53feTest

المؤلفون: Osman Evliyaoglu, Ralf Weiskirchen, Josef van Helden, Mathias Imöhl

المصدر: Clinical Chemistry and Laboratory Medicine (CCLM). 58:1291-1301

مصطلحات موضوعية: Adult, Anti-Mullerian Hormone, endocrine system, medicine.medical_specialty, Adolescent, Clinical Biochemistry, Youden's J statistic, 030209 endocrinology & metabolism, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Reference Values, Tandem Mass Spectrometry, Internal medicine, medicine, Humans, Testosterone, 030219 obstetrics & reproductive medicine, Estradiol, Free androgen index, business.industry, Polycystic ovary syndrome (PCOS), Biochemistry (medical), Age Factors, General Medicine, Luteinizing Hormone, Middle Aged, medicine.disease, Age specific, Polycystic ovary, Endocrinology, Reference values, Female, Follicle Stimulating Hormone, Luteinizing hormone, business, Chromatography, Liquid, Polycystic Ovary Syndrome, Hormone

الوصف: Background The increased secretion of anti-Müllerian hormone (AMH) by the growing follicles has been supposed as a determinative feature of polycystic ovary syndrome (PCOS). The diagnostic performance of AMH in PCOS is superior compared to the free androgen index (FAI) and luteinizing hormone (LH)/follicle-stimulating hormone (FSH) quotient. We established age-dependent reference ranges to further improve the diagnostic performance of AMH. Methods In a cross-sectional study, in samples of 4712 reproductive age patients, ranging from 14 to 50 years, BMI, AMH and other reproductive hormones were determined by immunoassay or tandem mass spectrometry (LC-MS/MS) to calculate age-specific reference ranges and the diagnostic performance. Results Age-specific diagnostic performances for Elecsys® AMH, FAI and LH/FSH ratio were established in the reference group. No significant difference in BMI was found between the groups. AMH values were significantly negatively correlated with age (r = −0.628, p Conclusions We determined the age-dependent reference ranges for serum AMH levels in a large population-based study and calculated the age-specific diagnostic performance of FAI and LH/FSH ratio, which allows physicians to evaluate patients with PCOS who have normal AMH levels. AMH is suggested as the strongest diagnostic marker in patients with PCOS compared to FAI and LH/FSH ratio.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::875967724c81c8c8300b21959d035c31Test
https://doi.org/10.1515/cclm-2019-1059Test

المؤلفون: Cemal Polat, Osman Evliyaoğlu, Revşa Evin Canpolat Erkan, Ahmet Baştürk, Tugrul Çakir, Arif Aslaner, Şükran Can, Nuriye Mete

المصدر: American Journal of Experimental and Clinical Research, Vol 2, Iss 3, Pp 118-120 (2015)

مصطلحات موضوعية: Urinary tract infection, leukocyte number, sensitivity, specificity, microscopy, Medicine

الوصف: Urinary tract infections are the diseases of the urogenital system caused by various microorganisms. Currently, the most used descriptive tests are urine strips, microscopic analysis of urine and spot bacteruria tests. The aim of this study was to present the consistency of culture results with leukocyte count determined cytometrically in the urine of patients considered to have a urinary tract infection and thereby facilitate treatment approaches. A retrospective examination was performed with the urine samples of patients sent to central laboratory of the Dicle University Hospital in January2012- December 2013. Both microscopic urine analysis and urine culture were performed for each patient on the same day. The study comprised a total of 839 patients; 222 males and 617 females. Urine culture results and urine microscopy findings of patients with positive urine strip tests were compared. With the comparison of urine culture results, positive urine microscopy findings were found to have 92%sensitivity, 26% specificity, 52% positive predictive value (PPV)and 78% negative predictive value (NPV) (p<0.001).Compared to the culture results, the urine microscopy findings of patients with positive urine strip tests were found to have high sensitivity and low specificity (p<0.001). As a result empirical antimicrobial therapy can be considered for patients with positive urine microscopy findings without waiting for culture results, and patients with negative results are recommended to have urine culture results.

العلاقة: http://journals.sfu.ca/ajecr/index.php/ajecr/article/view/46Test; https://doaj.org/toc/2330-9237Test; https://doaj.org/toc/2330-9245Test; https://doaj.org/article/5832b7f854164245a8c8c821b7edf099Test

الإتاحة: https://doaj.org/article/5832b7f854164245a8c8c821b7edf099Test

المؤلفون: İbrahim Kaplan, Hatice Yüksel, Osman Evliyaoğlu, M. Kemal Basarali, Gülten Toprak, Leyla Çolpan, Velat Şen

المصدر: International Journal of Analytical Chemistry, Vol 2015 (2015)

مصطلحات موضوعية: Analytical chemistry, QD71-142

الوصف: Tacrolimus and cyclosporine A are immunosuppressant drugs with narrow therapeutic windows. The aim of this study was to investigate the stability of tacrolimus and cyclosporin A levels in whole blood samples under different storage conditions. Whole blood samples were obtained from 15 patients receiving tacrolimus and 15 patients receiving cyclosporine A. Samples were immediately analyzed and then stored at different conditions (room temperature (24°C−26°C) for 24 hours, +4°C for 24 and 48 hours, and −20°C for one month) and then analyzed again. For tacrolimus, there was a significant difference between samples analyzed immediately and those kept 24 hours at room temperature (P=0.005) (percent change 32.89%). However, there were no significant differences between the other groups. For cyclosporine A, there was a significant difference between samples analyzed immediately and those kept 24 hours (P=0.003) (percent change 19.47%) and 48 hours (P=0.002) (percent change 15.38%) at +4°C and those kept 24 hours at room temperature (P=0.011) (percent change 9.71%). Samples of tacrolimus should be analyzed immediately or stored at either +4°C or −20°C, while samples of cyclosporine A should be analyzed immediately or stored at −20°C.

العلاقة: http://dx.doi.org/10.1155/2015/956389Test; https://doaj.org/toc/1687-8760Test; https://doaj.org/toc/1687-8779Test; https://doaj.org/article/19164e917b944900bea1ba36cd832dc7Test

الإتاحة: https://doi.org/10.1155/2015/956389Test
https://doaj.org/article/19164e917b944900bea1ba36cd832dc7Test

المؤلفون: Matthias Imöhl, Ralf Weiskirchen, Osman Evliyaoglu, Josef van Helden

المصدر: Nutrition. 60:25-29

مصطلحات موضوعية: 0301 basic medicine, Vitamin, 030109 nutrition & dietetics, Nutrition and Dietetics, medicine.diagnostic_test, business.industry, Endocrinology, Diabetes and Metabolism, Physiology, Complete blood count, 030209 endocrinology & metabolism, Reference intervals, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, chemistry, Medicine, Hemoglobin, business, Whole blood

الوصف: Objectives The aims of this study were to underline the interpretation of vitamin B1 and to evaluate whether differences in hemoglobin (Hb) levels and sex effect vitamin B1 results. Methods Simultaneously, whole blood vitamin B1 and complete blood count were determined in 2238 individuals. Groups were categorized on the basis of sex and Hb levels. Significance and correlation between groups and reference intervals of the study group were determined. Results There was an 8.4% (P Conclusion Vitamin B1 concentrations >48 μg/ L should be interpreted with Hb levels to avoid postanalytical errors that mask deficiency. Therefore, in comparative studies, researchers need to pay attention to eliminate the effect of Hb on whole blood vitamin B1 levels.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::049b8c0b448faa4973efff91cbe68978Test
https://doi.org/10.1016/j.nut.2018.09.002Test