المؤلفون: Doliba, Nicolai M., Babsky, Andriy M., Osbakken, Mary D.

المصدر: Frontiers in Physiology ; volume 9 ; ISSN 1664-042X

مصطلحات موضوعية: Physiology (medical), Physiology

الإتاحة: https://doi.org/10.3389/fphys.2018.01473Test

المؤلفون: Friedman, Mark I., Graczyk-Millbrandt, Grazyna, Ji, Hong, Rawson, Nancy E., Osbakken, Mary D.

المصدر: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research ; volume 1642, issue 1-2, page 53-58 ; ISSN 0167-4889

مصطلحات موضوعية: Cell Biology, Molecular Biology

الإتاحة: https://doi.org/10.1016/s0167-4889Test(03)00098-3
https://api.elsevier.com/content/article/PII:S0167488903000983?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0167488903000983?httpAccept=text/plainTest

المؤلفون: Doliba, Nicolai M., Babsky, Andriy M., Doliba, Nataliya M., Wehrli, Suzanne L., Osbakken, Mary D.

المصدر: Cell Biochemistry and Function ; volume 33, issue 2, page 67-72 ; ISSN 0263-6484 1099-0844

الوصف: Adenylate kinase plays an important role in cellular energy homeostasis by catalysing the interconversion of adenine nucleotides. The goal of present study was to evaluate the contribution of the adenylate kinase reaction to oxidative ATP synthesis by direct measurements of ATP using 31 P NMR spectroscopy. Results show that AMP can stimulate ATP synthesis in the presence or absence of ADP. In particular, addition of 1 mM AMP to the 0.6 mM ADP superfusion system of isolated superfused mitochondria (contained and maintained in agarose beads) led to a 25% increase in ATP synthesis as measured by the increase in β ATP signal. More importantly, we show that AMP can support ATP synthesis in the absence of ADP, demonstrated as follows. Superfusion of mitochondria without ADP led to the disappearance of ATP γ , α and β signals and the increase of P i . Addition of AMP to the medium restored the production of ATP, as demonstrated by the reappearance of γ , α and β ATP signals, in conjunction with a decrease in P i , which is being used for ATP synthesis. Polarographic studies showed Mg 2+ dependence of this process, confirming the specificity of the adenylate kinase reaction. Furthermore, data obtained from this study demonstrate, for the first time, that different aspects of the adenylate kinase reaction can be evaluated with 31 P NMR spectroscopy. Copyright © 2015 John Wiley & Sons, Ltd. SIGNIFICANCE OF RESEARCH PARAGRAPH The data generated in the present study indicate that 31 P NMR spectroscopy can effectively be used to study the adenylate kinase reaction under a variety of conditions. This is important because understanding of adenylate kinase function and/or malfunction is essential to understanding its role in health and disease. The data obtained with 31 P NMR were confirmed by polarographic studies, which further strengthens the robustness of the NMR findings. In summary, 31 P NMR spectroscopy provides a sensitive tool to study adenylate kinase activity in different physiological and pathophysiological ...

الإتاحة: https://doi.org/10.1002/cbf.3089Test

المؤلفون: Malmqvist, Karin, Kahan, Thomas, Edner, Magnus, Held, Claes, Hägg, Anders, Lind, Lars, Muller-Brunotte, Richard, Nyström, Fredrik, Öhman, K. Peter, Osbakken, Mary D., Östergern, Jan

المصدر: Journal of Hypertension. 19(6):1167-1176

مصطلحات موضوعية: Adrenergic beta-Antagonists/adverse effects/therapeutic use, Adult, Aged, Atenolol/adverse effects/therapeutic use, Biphenyl Compounds/adverse effects/*therapeutic use, Blood Pressure/drug effects, Double-Blind Method, Female, Heart Rate/drug effects, Humans, Hypertension/complications/*drug therapy/pathology/physiopathology, Hypertrophy, Left Ventricular/complications/*drug therapy/pathology/physiopathology, Male, Middle Aged, Receptor, Angiotensin, Type 1, Receptors, Angiotensin/*antagonists & inhibitors, Research Support, Non-U.S. Gov't, Safety, Tetrazoles/adverse effects/*therapeutic use, Vascular Resistance/drug effects, MEDICINE, MEDICIN

الوصف: BACKGROUND: The Swedish irbesartan left ventricular hypertrophy investigation versus atenolol (SILVHIA). OBJECTIVE: Angiotensin II induces myocardial hypertrophy. We hypothesized that blockade of angiotensin II subtype 1 (AT1) receptors by the AT1-receptor antagonist irbesartan would reduce left ventricular mass (as measured by echocardiography) more than conventional treatment with a beta blocker. DESIGN AND METHODS: This double-blind study randomized 115 hypertensive men and women with left ventricular hypertrophy to receive either irbesartan 150 mg q.d. or atenolol 50 mg q.d. for 48 weeks. If diastolic blood pressure remained above 90 mmHg, doses were doubled, and additional medications (hydrochlorothiazide and felodipine) were prescribed as needed. Echocardiography was performed at weeks 0, 12, 24 and 48. RESULTS: Baseline mean blood pressure was 162/ 104 mmHg, and mean left ventricular mass index was 157 g/m2 for men and 133 g/m2 for women. Systolic and diastolic blood pressure reductions were similar in both treatment groups. Both irbesartan (P < 0.001) and atenolol (P< 0.001) progressively reduced left ventricular mass index, e.g. by 26 and 14 g/m2 (16 and 9%), respectively, at week 48, with a greater reduction in the irbesartan group (P = 0.024). The proportion of patients who attained a normalized left ventricular mass (i.e. < or = 131 g/m2 for men and < or = 100 g/m2 for women) tended to be greater with irbesartan (47 versus 32%, P = 0.108). CONCLUSIONS: Left ventricular mass was reduced more in the irbesartan group than in the atenolol group. These results suggest that blocking the action of angiotensin II at AT1-receptors may be an important mechanism, beyond that of lowering blood pressure, in the regulation of left ventricular mass and geometry in patients with hypertension.

وصف الملف: print

الوصول الحر: https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-73124Test
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=11403367&dopt=CitationTest

المؤلفون: Crestanello, Juan A., Doliba, Nicolai M., Doliba, Natalia M., Babsky, Andriy M., Niborii, Koki, Osbakken, Mary D., Whitman, Glenn J.R.

المصدر: Journal of Surgical Research ; volume 102, issue 2, page 221-228 ; ISSN 0022-4804

مصطلحات موضوعية: Surgery

الإتاحة: https://doi.org/10.1006/jsre.2001.6324Test
https://api.elsevier.com/content/article/PII:S0022480401963243?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0022480401963243?httpAccept=text/plainTest

المؤلفون: Crestanello, Juan A., Doliba, Nicolai M., Babsky, Andriy M., Doliba, Natalia M., Niibori, Koki, Whitman, Glenn J.R., Osbakken, Mary D.

المصدر: Journal of Surgical Research ; volume 103, issue 2, page 243-251 ; ISSN 0022-4804

مصطلحات موضوعية: Surgery

الإتاحة: https://doi.org/10.1006/jsre.2001.6361Test
https://api.elsevier.com/content/article/PII:S0022480401963619?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0022480401963619?httpAccept=text/plainTest

المؤلفون: Crestanello, Juan A., Doliba, Nicolai M., Babsky, Andriy M., Doliba, Natalia M., Niibori, Koki, Osbakken, Mary D., Whitman, Glenn J.R.

المصدر: Surgery ; volume 131, issue 2, page 172-178 ; ISSN 0039-6060

مصطلحات موضوعية: Surgery

الإتاحة: https://doi.org/10.1067/msy.2002.119490Test
https://api.elsevier.com/content/article/PII:S0039606002118204?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0039606002118204?httpAccept=text/plainTest

المؤلفون: Crestanello, Juan A., Doliba, Nicolai M., Babsky, Andriy M., Doliba, Natalia M., Niibori, Koki, Osbakken, Mary D., Whitman, Glenn J.R.

المصدر: Journal of Surgical Research ; volume 94, issue 2, page 116-123 ; ISSN 0022-4804

مصطلحات موضوعية: Surgery

الإتاحة: https://doi.org/10.1006/jsre.2000.5979Test
https://api.elsevier.com/content/article/PII:S0022480400959791?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0022480400959791?httpAccept=text/plainTest

المؤلفون: Doliba, Nicolai M., Wehrli, Suzanne L., Babsky, Andriy M., Doliba, Nataliya M., Osbakken, Mary D.

المصدر: Magnetic Resonance in Medicine ; volume 39, issue 5, page 679-684 ; ISSN 0740-3194 1522-2594

الوصف: An NMR method to study on‐line mitochondrial function was developed. Mitochondria were maintained in a stable physiologic state in agarose beads that were continuously superfused with oxygenated buffer at 28°C. Oxidative function of both heart and liver mitochondria was evaluated with 31 P NMR at 9.4 T using pyruvate plus malate as substrate. This method allows clear resolution of adenosine triphosphate‐γ (ATPγ) and adenosine diphosphate‐β (ADPβ) phosphate signals, whereas a signals of ATP and ADP overlap. ATP production by mitochondria was documented to be very sensitive to different interventions (hypoxia, ischemia, carbonyl cyanide p‐(tri‐fluoromethoxy)phenylhydrazone (FCCP)) and depended on the ADP concentration in supervision medium. These data demonstrate that the new application of NMR to study mitochondrial function can discriminate, on‐line, between several physiologic and biochemical processes in intact physiologically stable mitochondria.

الإتاحة: https://doi.org/10.1002/mrm.1910390502Test

المؤلفون: O'Neill, Martin J., Pierce, William S., Wisman, Craig B., Osbakken, Mary D., Parr, Grant V.S., Waldhausen, John A.

المصدر: The Journal of Thoracic and Cardiovascular Surgery ; volume 87, issue 1, page 106-111 ; ISSN 0022-5223

مصطلحات موضوعية: Cardiology and Cardiovascular Medicine, Pulmonary and Respiratory Medicine, Surgery

الإتاحة: https://doi.org/10.1016/s0022-5223Test(19)37449-5
https://api.elsevier.com/content/article/PII:S0022522319374495?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0022522319374495?httpAccept=text/plainTest