المؤلفون: Wang, G, Hallberg, J, Charalampopoulos, D, Sanahuja, MC, Breyer-Kohansal, R, Langhammer, A, Granell, R, Vonk, JM, Mian, A, Olvera, N, Laustsen, LM, Ronmark, E, Abellan, A, Agusti, A, Arshad, SH, Bergstrom, A, Boezen, HM, Breyer, MK, Burghuber, O, Bolund, AC, Custovic, A, Devereux, G, Donaldson, GC, Duijts, L, Esplugues, A, Faner, R, Ballester, F, Garcia-Aymerich, J, Gehring, U, Haider, S, Hartl, S, Backman, H, Holloway, JW, Koppelman, GH, Lertxundi, A, Holmen, TL, Lowe, L, Mensink-Bout, SM, Murray, CS, Roberts, G, Hedman, L, Schlunssen, V, Sigsgaard, T, Simpson, A, Sunyer, J, Torrent, M, Turner, S, Van den Berge, M, Vermeulen, RCH, Vikjord, SAA, Wedzicha, JA, van der Zee, AHM, Melen, E

المصدر: ERJ open research. 7(4)

مصطلحات موضوعية: Medicin och hälsovetenskap

الوصول الحر: http://kipublications.ki.se/Default.aspx?queryparsed=id:149174473Test

المؤلفون: Bertels, X, Edris, A, Garcia-Aymerich, J, Faner, R, Meteran, H, Sigsgaard, T, Alter, P, Vogelmeier, C, Olvera, N, Kermani, NZ, Agusti, A, Donaldson, GC, Wedzicha, JA, Brusselle, GG, Backman, H, Ronmark, E, Lindberg, A, Vonk, JM, Chung, KF, Adcock, IM, Van den Berge, M, Lahousse, L

الوصف: Background The prevalence and clinical profile of asthma with airflow obstruction (AO) remain uncertain. We aimed to phenotype AO in population- and clinic-based cohorts. Methods This cross-sectional multicohort study included adults ≥50 years from nine CADSET cohorts with spirometry data (N=69 789). AO was defined as ever diagnosed asthma with pre-BD or post-BD FEV1/FVC <0.7 in population-based and clinic-based cohorts, respectively. Clinical characteristics and comorbidities of AO were compared with asthma without airflow obstruction (asthma-only) and chronic obstructive pulmonary disease (COPD) without asthma history (COPD-only). ORs for comorbidities adjusted for age, sex, smoking status and body mass index (BMI) were meta-analysed using a random effects model. Results The prevalence of AO was 2.1% (95% CI 2.0% to 2.2%) in population-based, 21.1% (95% CI 18.6% to 23.8%) in asthma-based and 16.9% (95% CI 15.8% to 17.9%) in COPD-based cohorts. AO patients had more often clinically relevant dyspnoea (modified Medical Research Council score ≥2) than asthma-only (+14.4 and +14.7 percentage points) and COPD-only (+24.0 and +5.0 percentage points) in population-based and clinic-based cohorts, respectively. AO patients had more often elevated blood eosinophil counts (>300 cells/µL), although only significant in population-based cohorts. Compared with asthma-only, AO patients were more often men, current smokers, with a lower BMI, had less often obesity and had more often chronic bronchitis. Compared with COPD-only, AO patients were younger, less often current smokers and had less pack-years. In the general population, AO patients had a higher risk of coronary artery disease than asthma-only and COPD-only (OR=2.09 (95% CI 1.26 to 3.47) and OR=1.89 (95% CI 1.10 to 3.24), respectively) and of depression (OR=1.41 (95% CI 1.19 to 1.67)), osteoporosis (OR=2.30 (95% CI 1.43 to 3.72)) and gastro-oesophageal reflux disease (OR=1.68 (95% CI 1.06 to 2.68)) than COPD-only, independent of age, sex, smoking status and BMI. ...

العلاقة: BMJ Open Respiratory Research; http://hdl.handle.net/10044/1/107904Test

الإتاحة: https://doi.org/10.1136/bmjresp-2023-001760Test
http://hdl.handle.net/10044/1/107904Test

المؤلفون: Ramos-García, J., Robles-Rivera, F., Chávez-Soto, M., Valdés, M., Calzada, F., Ortiz-Olvera, N.

المساهمون: Fundación IMSS AC

المصدر: Revista de Gastroenterología de México ; volume 88, issue 2, page 85-90 ; ISSN 0375-0906

مصطلحات موضوعية: Gastroenterology

الإتاحة: https://doi.org/10.1016/j.rgmx.2021.05.007Test
https://api.elsevier.com/content/article/PII:S0375090621001245?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0375090621001245?httpAccept=text/plainTest

المؤلفون: Ramos-García, J., Robles-Rivera, F., Chávez-Soto, M., Valdés, M., Calzada, F., Ortiz-Olvera, N.

المصدر: Revista de Gastroenterología de México (English Edition) ; volume 88, issue 2, page 85-90 ; ISSN 2255-534X

مصطلحات موضوعية: General Medicine

الإتاحة: https://doi.org/10.1016/j.rgmxen.2022.03.005Test
https://api.elsevier.com/content/article/PII:S2255534X22000184?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2255534X22000184?httpAccept=text/plainTest

المؤلفون: Aguilar-Zamora, E, Weimer, BC, Torres, RC, Gomez-Delgado, A, Ortiz-Olvera, N, Aparicio-Ozores, G, Barbero-Becerra, VJ, Torres, J, Camorlinga-Ponce, M

الوصف: Clostridioides difficile is a global public health problem, which is a primary cause of antibiotic-associated diarrhea in humans. The emergence of hypervirulent and antibiotic-resistant strains is associated with the increased incidence and severity of the disease. There are limited studies on genomic characterization of C. difficile in Latin America. We aimed to learn about the molecular epidemiology and antimicrobial resistance in C. difficile strains from adults and children in hospitals of México. We studied 94 C. difficile isolates from seven hospitals in Mexico City from 2014 to 2018. Whole-genome sequencing (WGS) was used to determine the genotype and examine the toxigenic profiles. Susceptibility to antibiotics was determined by E-test. Multilocus sequence typing (MLST) was used to determine allelic profiles. Results identified 20 different sequence types (ST) in the 94 isolates, mostly clade 2 and clade 1. ST1 was predominant in isolates from adult and children. Toxigenic strains comprised 87.2% of the isolates that were combinations of tcdAB and cdtAB (tcdA+/tcdB+/cdtA+/cdtB+, followed by tcdA+/tcdB+/cdtA-/cdtB-, tcdA-/tcdB+/cdtA-/ cdtB-, and tcdA-/tcdB-/cdtA+/cdtB+). Toxin profiles were more diverse in isolates from children. All 94 isolates were susceptible to metronidazole and vancomycin, whereas a considerable number of isolates were resistant to clindamycin, fluroquinolones, rifampicin, meropenem, and linezolid. Multidrug-resistant isolates (≥3 antibiotics) comprised 65% of the isolates. The correlation between resistant genotypes and phenotypes was evaluated by the kappa test. Mutations in rpoB and rpoC showed moderate concordance with resistance to rifampicin and mutations in fusA substantial concordance with fusidic acid resistance. cfrE, a gene recently described in one Mexican isolate, was present in 65% of strains linezolid resistant, all ST1 organisms. WGS is a powerful tool to genotype and characterize virulence and antibiotic susceptibility patterns.

العلاقة: Aguilar-Zamora, E., Weimer, B. C., Torres, R. C., Gomez-Delgado, A., Ortiz-Olvera, N., Aparicio-Ozores, G., Barbero-Becerra, V. J., Torres, J. & Camorlinga-Ponce, M. (2022). Molecular Epidemiology and Antimicrobial Resistance of Clostridioides difficile in Hospitalized Patients From Mexico. FRONTIERS IN MICROBIOLOGY, 12, https://doi.org/10.3389/fmicb.2021.787451Test.; http://hdl.handle.net/11343/334360Test

الإتاحة: https://doi.org/10.3389/fmicb.2021.787451Test
http://hdl.handle.net/11343/334360Test

المؤلفون: Bell, D, Berchuck, A, Birrer, M, Chien, J, Cramer, DW, Dao, F, Dhir, R, DiSaia, P, Gabra, H, Glenn, P, Godwin, AK, Gross, J, Hartmann, L, Huang, M, Huntsman, DG, Iacocca, M, Imielinski, M, Kalloger, S, Karlan, BY, Levine, DA, Mills, GB, Morrison, C, Mutch, D, Olvera, N, Orsulic, S, Park, K, Petrelli, N, Rabeno, B, Rader, JS, Sikic, BI, Smith-McCune, K, Sood, AK, Bowtell, D, Penny, R, Testa, JR, Chang, K, Creighton, CJ, Dinh, HH, Drummond, JA, Fowler, G, Gunaratne, P, Hawes, AC, Kovar, CL, Lewis, LR, Morgan, MB, Newsham, IF, Santibanez, J, Reid, JG, Trevino, LR, Wu, Y-Q, Wang, M, Muzny, DM, Wheeler, DA, Gibbs, RA, Getz, G, Lawrence, MS, Cibulskis, K, Sivachenko, AY, Sougnez, C, Voet, D, Wilkinson, J, Bloom, T, Ardlie, K, Fennell, T, Baldwin, J, Nichol, R, Fisher, S, Gabriel, S, Lander, ES, Ding, L, Fulton, RS, Koboldt, DC, McLellan, MD, Wylie, T, Walker, J, O’Laughlin, M, Dooling, DJ, Fulton, L, Abbott, R, Dees, ND, Zhang, Q, Kandoth, C, Wendl, M, Schierding, W, Shen, D, Harris, CC, Schmidt, H, Kalicki, J, Delehaunty, KD, Fronick, CC, Demeter, R, Cook, L, Wallis, JW, Lin, L, Magrini, VJ, Hodges, JS, Eldred, JM, Smith, SM, Pohl, CS, Vandin, F

المصدر: Nature. 474(7353)

مصطلحات موضوعية: Biological Sciences, Biomedical and Clinical Sciences, Genetics, Oncology and Carcinogenesis, Biotechnology, Rare Diseases, Human Genome, Ovarian Cancer, Cancer, Aged, Carcinoma, DNA Methylation, Female, Gene Dosage, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Genomics, Humans, MicroRNAs, Middle Aged, Mutation, Ovarian Neoplasms, RNA, Messenger, Cancer Genome Atlas Research Network, General Science & Technology

الوصف: A catalogue of molecular aberrations that cause ovarian cancer is critical for developing and deploying therapies that will improve patients' lives. The Cancer Genome Atlas project has analysed messenger RNA expression, microRNA expression, promoter methylation and DNA copy number in 489 high-grade serous ovarian adenocarcinomas and the DNA sequences of exons from coding genes in 316 of these tumours. Here we report that high-grade serous ovarian cancer is characterized by TP53 mutations in almost all tumours (96%); low prevalence but statistically recurrent somatic mutations in nine further genes including NF1, BRCA1, BRCA2, RB1 and CDK12; 113 significant focal DNA copy number aberrations; and promoter methylation events involving 168 genes. Analyses delineated four ovarian cancer transcriptional subtypes, three microRNA subtypes, four promoter methylation subtypes and a transcriptional signature associated with survival duration, and shed new light on the impact that tumours with BRCA1/2 (BRCA1 or BRCA2) and CCNE1 aberrations have on survival. Pathway analyses suggested that homologous recombination is defective in about half of the tumours analysed, and that NOTCH and FOXM1 signalling are involved in serous ovarian cancer pathophysiology.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/44b1h9tbTest

المؤلفون: Hernandez-Pacheco, N, Kilanowski, A, Kumar, A, Curtin, JA, Olvera, N, Kress, S, Bertels, X, Lahousse, L, Bhatta, L, Granell, R, Mari, S, Bilbao, JR, Sun, YD, Karramass, T, Merid, SK, Wang, G, Hallberg, J, Casas, M, Garcia-Aymerich, J, Bustamante, M, Pershagen, G, Georgelis, A, Lowe, L, Simpson, A, Gehring, U, Vermeulen, RC, Sigsgaard, T, Schlunssen, V, Roberts, G, Bergstrom, A, Vonk, JM, Felix, J, Duijts, L, Timpson, N, Brusselle, G, Brumpton, B, Langhammer, A, Turner, S, Holloway, JW, Arshad, SH, Custovic, A, Cullinan, P, Murray, CS, Van den Berge, M, Kull, I, Schikowski, T, Wedzicha, JA, Koppelman, G, Faner, R, Agusti, A, Standl, M, Melen, E

المصدر: EUROPEAN RESPIRATORY JOURNAL. 62

مصطلحات موضوعية: Medicin och hälsovetenskap

الوصول الحر: http://kipublications.ki.se/Default.aspx?queryparsed=id:154588356Test

المؤلفون: Ruiz-Garcia, E., Fernandez-Figueroa, E., Avedaño, A. Moreno, Navarrete, U. Coquis, Falcon, J., Manriquez, D. Ramirez, Rios, C. Garcia, Fuentes, S. Velasco, Cardenas-Delgado, I., Takahashi, A. Leon, Diaz, C., Ortiz-Olvera, N., Kanonnikoff, T. Fleitas

المصدر: Annals of Oncology ; volume 34, page S158 ; ISSN 0923-7534

مصطلحات موضوعية: Oncology, Hematology

الإتاحة: https://doi.org/10.1016/j.annonc.2023.04.470Test
https://api.elsevier.com/content/article/PII:S0923753423006129?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0923753423006129?httpAccept=text/plainTest

المؤلفون: Canals, R., Chaudhuri, R.R., Steiner, R.E., Owen, S.V., Quinones-Olvera, N., Gordon, M.A., Baym, M., Ibba, M., Hinton, J.C.D.

الوصف: We have used a transposon insertion sequencing (TIS) approach to establish the fitness landscape of the African Salmonella enterica serovar Typhimurium ST313 strain D23580, to complement our previous comparative genomic and functional transcriptomic studies. We used a genome-wide transposon library with insertions every 10 nucleotides to identify genes required for survival and growth in vitro and during infection of murine macrophages. The analysis revealed genomic regions important for fitness under two in vitro growth conditions. Overall, 724 coding genes were required for optimal growth in LB medium, and 851 coding genes were required for growth in SPI-2-inducing minimal medium. These findings were consistent with the essentiality analyses of other S. Typhimurium ST19 and S. Typhi strains. The global mutagenesis approach also identified 60 sRNAs and 413 intergenic regions required for growth in at least one in vitro growth condition. By infecting murine macrophages with the transposon library, we identified 68 genes that were required for intra-macrophage replication but did not impact fitness in vitro. None of these genes were unique to S. Typhimurium D23580, consistent with a high conservation of gene function between S. Typhimurium ST313 and ST19 and suggesting that novel virulence factors are not involved in the interaction of strain D23580 with murine macrophages. We discovered that transposon insertions rarely occurred in many pBT1 plasmid-encoded genes (36), compared with genes carried by the pSLT-BT virulence plasmid and other bacterial plasmids. The key essential protein encoded by pBT1 is a cysteinyl-tRNA synthetase, and our enzymological analysis revealed that the plasmid-encoded CysRSpBT1 had a lower ability to charge tRNA than the chromosomally-encoded CysRSchr enzyme. The presence of aminoacyl-tRNA synthetases in plasmids from a range of Gram-negative and Gram-positive bacteria suggests that plasmid-encoded essential genes are more common than had been appreciated.

وصف الملف: text

العلاقة: https://eprints.whiterose.ac.uk/151567/1/journal.ppat.1007948.pdfTest; Canals, R., Chaudhuri, R.R. orcid.org/0000-0001-5037-2695 , Steiner, R.E. et al. (6 more authors) (2019) The fitness landscape of the African salmonella typhimurium ST313 strain D23580 reveals unique properties of the pBT1 plasmid. PLOS Pathogens, 15 (9).

الإتاحة: https://eprints.whiterose.ac.uk/151567Test/
https://eprints.whiterose.ac.uk/151567/1/journal.ppat.1007948.pdfTest

المؤلفون: Obrador Vera, G., Villa, A., Cuadra, M., De Arrigunaga, S., Cárdenas, C., Bracho, M., Sotelo, A., Olvera, N., Guitian, M., Urquiza, M.

المصدر: Kidney International Reports ; volume 4, issue 7, page S245 ; ISSN 2468-0249

مصطلحات موضوعية: Nephrology

الإتاحة: https://doi.org/10.1016/j.ekir.2019.05.610Test
https://api.elsevier.com/content/article/PII:S246802491930806X?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S246802491930806X?httpAccept=text/plainTest