المؤلفون: Scandiani, María Mercedes, Ruberti, Delma S., Giorda, Laura M., Pioli, Rosanna N., Luque, Alicia G., Bottai, Hebe, Ivancovich, Juan J., Aoki, Takayuki, O'Donnell, Kerry

المصدر: Tropical Plant Pathology. June 2011 36(3)

مصطلحات موضوعية: Glycine max, Argentina, pathogenicity, SDS, United States

الوصف: Fusarium tucumaniae and F. virguliforme are the primary etiological agents of sudden-death syndrome (SDS) of soybean in Argentina and the United States, respectively. Five isolates of F. tucumaniae and four isolates of F. virguliforme were tested for relative aggressiveness to soybean, using a toothpick inoculation method and two versions of a soil infestation inoculation method. Partially resistant soybean cultivar RA629 and susceptible cultivar A6445RG were inoculated separately with each of the nine isolates. Two experiments for each inoculation method were performed. Analysis of variance identified a significant three-way interaction of soybean cultivar*experiment*SDS pathogen (P = 0.01) using the different methods. When the two soil infestation methods were used, F. virguliforme was more aggressive than F. tucumaniae; however, when using the toothpick method, isolates of F. virguliforme and F. tucumaniae were equally aggressive. Although all three methods discriminated levels of partial resistance of the genotypes to SDS, results of the present study indicated that soil inoculations with sorghum infested grain represent the best method for evaluating soybean cultivar resistance to SDS. The existence of interactions among the host, pathogen and environmental conditions highlights the need for additional studies to improve the reproducibility of tests for screening soybean germplasm for resistance to SDS.

وصف الملف: text/html

الوصول الحر: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762011000300001Test

المؤلفون: Martinelli, José A., Bocchese, Carla A.C., Xie, Weiping, O'Donnell, Kerry, Kistler, Harold C.

المصدر: Fitopatologia Brasileira. October 2004 29(5)

مصطلحات موضوعية: Glycine max, Gibberella zeae, pathogenicity, Deoxinivalenol, Nivalenol, compatibility groups

الوصف: Surveys of soybean (Glycine max) seed grown in South Brazil revealed infection with Fusarium graminearum. To determine if members of this complex were pathogenic to soybean, six strains derived from soybean were added to soil at a rate of 10³ macroconidia/ ml or individual pods were inoculated with 10(4) macroconidia/ml. Seedlings grown in infested soil developed small necrotic lesions in the crown and upper roots. Pods inoculated with conidia developed large (>1 cm), dark brown, necrotic lesions. Younger pods inoculated with the fungus blighted and dropped from the plant. Strains of the F. graminearum complex recovered from lesions on the crown, roots and pods of soybean plants were identified as lineage 1, 2 or 8 by obtaining the DNA sequence from the EF1-alpha gene and comparing it to strains of the known lineage. Two strains of F. graminearum lineage 7 from the U.S. caused similar symptoms of the disease on soybean. Mycotoxin tests on soybean and wheat (Triticum aestivum) indicate that most Brazilian strains produce nivalenol as the major trichothecene mycotoxin rather than deoxynivalenol. In addition, strains from lineages 2 and 8 produce the novel trichothecene, 3-acetylnivalenol.

وصف الملف: text/html

الوصول الحر: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000500004Test

المؤلفون: Chang, Ying, Wang, Yan, Mondo, Stephen, Ahrendt, Steven, Andreopoulos, William, Barry, Kerrie, Beard, Jeff, Benny, Gerald L, Blankenship, Sabrina, Bonito, Gregory, Cuomo, Christina, Desiro, Alessandro, Gervers, Kyle A, Hundley, Hope, Kuo, Alan, LaButti, Kurt, Lang, B Franz, Lipzen, Anna, O'Donnell, Kerry, Pangilinan, Jasmyn, Reynolds, Nicole, Sandor, Laura, Smith, Matthew E, Tsang, Adrian, Grigoriev, Igor V, Stajich, Jason E, Spatafora, Joseph W

المصدر: iScience. 25(8)

مصطلحات موضوعية: Microbiology, Biological Sciences, Evolutionary Biology, Biological sciences, microbial genomics, microbial metabolism, microbiology, omics

الوصف: Fungi survive in diverse ecological niches by secreting proteins and other molecules into the environment to acquire food and interact with various biotic and abiotic stressors. Fungal secretome content is, therefore, believed to be tightly linked to fungal ecologies. We sampled 132 genomes from the early-diverging terrestrial fungal lineage zygomycetes (Mucoromycota and Zoopagomycota) and characterized their secretome composition. Our analyses revealed that phylogeny played an important role in shaping the secretome composition of zygomycete fungi with trophic mode contributing a smaller amount. Reconstruction of the evolution of secreted digestive enzymes revealed lineage-specific expansions, indicating that Mucoromycota and Zoopagomycota followed different trajectories early in their evolutionary history. We identified the presence of multiple pathogenicity-related proteins in the lineages known as saprotrophs, suggesting that either the ecologies of these fungi are incompletely known, and/or that these pathogenicity-related proteins have important functions associated with saprotrophic ecologies, both of which invite further investigation.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/68w9v9dvTest

المؤلفون: O’Donnell, Kerry, Al-Hatmi, Abdullah MS, Aoki, Takayuki, Brankovics, Balázs, Cano-Lira, José F, Coleman, Jeffrey J, de Hoog, G Sybren, Di Pietro, Antonio, Frandsen, Rasmus JN, Geiser, David M, Gibas, Connie FC, Guarro, Josep, Kim, Hye-Seon, Kistler, H Corby, Laraba, Imane, Leslie, John F, López-Berges, Manuel S, Lysøe, Erik, Meis, Jacques F, Monod, Michel, Proctor, Robert H, Rep, Martijn, Ruiz-Roldán, Carmen, Šišić, Adnan, Stajich, Jason E, Steenkamp, Emma T, Summerell, Brett A, van der Lee, Theo AJ, van Diepeningen, Anne D, Verweij, Paul E, Waalwijk, Cees, Ward, Todd J, Wickes, Brian L, Wiederhold, Nathan P, Wingfield, Michael J, Zhang, Ning, Zhang, Sean X

المصدر: mSphere. 5(5)

مصطلحات موضوعية: Microbiology, Biological Sciences, Infectious Diseases, Infection, Good Health and Well Being, Antifungal Agents, Fusarium, Phylogeny, clinical mycology, evolution, fungi, phylogenetics, taxonomy, Immunology

الوصف: This article is to alert medical mycologists and infectious disease specialists of recent name changes of medically important species of the filamentous mold FusariumFusarium species can cause localized and life-threating infections in humans. Of the 70 Fusarium species that have been reported to cause infections, close to one-third are members of the Fusarium solani species complex (FSSC), and they collectively account for approximately two-thirds of all reported Fusarium infections. Many of these species were recently given scientific names for the first time by a research group in the Netherlands, but they were misplaced in the genus Neocosmospora In this paper, we present genetic arguments that strongly support inclusion of the FSSC in Fusarium There are potentially serious consequences associated with using the name Neocosmospora for Fusarium species because clinicians need to be aware that fusaria are broadly resistant to the spectrum of antifungals that are currently available.

الوصول الحر: https://escholarship.org/uc/item/6bg317g9Test

المؤلفون: O'Donnell, Kerry, Al-Hatmi, Abdullah MS, Aoki, Takayuki, Brankovics, Balázs, Cano-Lira, José F, Coleman, Jeffrey J, de Hoog, G Sybren, Di Pietro, Antonio, Frandsen, Rasmus JN, Geiser, David M, Gibas, Connie FC, Guarro, Josep, Kim, Hye-Seon, Kistler, H Corby, Laraba, Imane, Leslie, John F, López-Berges, Manuel S, Lysøe, Erik, Meis, Jacques F, Monod, Michel, Proctor, Robert H, Rep, Martijn, Ruiz-Roldán, Carmen, Šišić, Adnan, Stajich, Jason E, Steenkamp, Emma T, Summerell, Brett A, van der Lee, Theo AJ, van Diepeningen, Anne D, Verweij, Paul E, Waalwijk, Cees, Ward, Todd J, Wickes, Brian L, Wiederhold, Nathan P, Wingfield, Michael J, Zhang, Ning, Zhang, Sean X

المصدر: mSphere. 5(5)

مصطلحات موضوعية: Fusarium, Antifungal Agents, Phylogeny, clinical mycology, evolution, fungi, phylogenetics, taxonomy, Infectious Diseases, Infection

الوصف: This article is to alert medical mycologists and infectious disease specialists of recent name changes of medically important species of the filamentous mold Fusarium Fusarium species can cause localized and life-threating infections in humans. Of the 70 Fusarium species that have been reported to cause infections, close to one-third are members of the Fusarium solani species complex (FSSC), and they collectively account for approximately two-thirds of all reported Fusarium infections. Many of these species were recently given scientific names for the first time by a research group in the Netherlands, but they were misplaced in the genus Neocosmospora In this paper, we present genetic arguments that strongly support inclusion of the FSSC in Fusarium There are potentially serious consequences associated with using the name Neocosmospora for Fusarium species because clinicians need to be aware that fusaria are broadly resistant to the spectrum of antifungals that are currently available.

الوصول الحر: https://escholarship.org/uc/item/6bg317g9Test

المؤلفون: Vandepol, Natalie, Liber, Julian, Desirò, Alessandro, Na, Hyunsoo, Kennedy, Megan, Barry, Kerrie, Grigoriev, Igor V, Miller, Andrew N, O'Donnell, Kerry, Stajich, Jason E, Bonito, Gregory

المصدر: Fungal diversity. 104(1)

مصطلحات موضوعية: Mortierellaceae, Phylogenomics, Molecular systematics, Taxonomy, Multi-locus sequence typing, Mycology & Parasitology, Evolutionary Biology, Microbiology, Plant Biology

الوصف: Early efforts to classify Mortierellaceae were based on macro- and micromorphology, but sequencing and phylogenetic studies with ribosomal DNA (rDNA) markers have demonstrated conflicting taxonomic groupings and polyphyletic genera. Although some taxonomic confusion in the family has been clarified, rDNA data alone is unable to resolve higher level phylogenetic relationships within Mortierellaceae. In this study, we applied two parallel approaches to resolve the Mortierellaceae phylogeny: low coverage genome (LCG) sequencing and high-throughput, multiplexed targeted amplicon sequencing to generate sequence data for multi-gene phylogenetics. We then combined our datasets to provide a well-supported genome-based phylogeny having broad sampling depth from the amplicon dataset. Resolving the Mortierellaceae phylogeny into monophyletic groups led to the definition of 14 genera, 7 of which are newly proposed. Low-coverage genome sequencing proved to be a relatively cost-effective means of generating a well-resolved phylogeny. The multi-gene phylogenetics approach enabled much greater sampling depth and breadth than the LCG approach, but was unable to resolve higher-level organization of groups. We present this work to resolve some of the taxonomic confusion and provide a genus-level framework to empower future studies on Mortierellaceae diversity, biology, and evolution.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/25k8j699Test

المؤلفون: Akhmetova, Galiya K., Knapp, Daniel G., Özer, Göksel, O'Donnell, Kerry, Laraba, Imane, Kiyas, Aldabergen

المساهمون: BAİBÜ, Ziraat Fakültesi, Bitki Koruma Bölümü, orcid:0000-0001-6896-8323, orcid:0000-0002-7568-238X, orcid:0000-0002-3385-2520, orcid:0000-0001-6507-691X, orcid:0000-0002-2118-8773, Özer, Göksel

مصطلحات موضوعية: CaM, Genealogical Concordance Phylogenetic Species Recognition (GCPSR), Morphology, Internal Transcribed Spacer, DNA-Sequence Database, Head Blight

الوصف: This research was supported partly by the ELTE Thematic Excellence Programme 2020 supported by National Research, Development and Innovation Office (TKP2020-IKA-05), the Hungarian-Chinese industrial research and development cooperation tender (2017-2.3.6-TET-CN-2018-00025), the National Research, Development and Innovation Office, Hungary (OTKA KH-130401 and K-139026), and the Stipendium Hungaricum Programme. D.G.K. was supported by a Janos Bolyai Research Scholarship from the Hungarian Academy of Sciences and Bolyai+ New National Excellence Program of the Ministry for Innovation and Technology. This research was supported in part by the U.S. Department of Agriculture Agricultural Research Service (ARS) National Program for Food Safety and the National Science Foundation (NSF DEB-1655980). I.L. was supported in part by an appointment to the ARS Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the U.S. Department of Agriculture (USDA). ORISE is managed by ORAU under DOE contract number DE-SC0014664. All opinions expressed in this paper are the authors' and do not necessarily reflect the policies and views of USDA, DOE, or ORAU/ORISE. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. ; In this study, DNA sequence data were used to characterize 290 Fusarium strains isolated during a survey of root-colonizing endophytic fungi of agricultural and nonagricultural plants in northern Kazakhstan. The Fusarium collection was screened for species identity using partial translation elongation factor 1-alpha (TEF1) gene sequences. Altogether, 16 different Fusarium species were identified, including eight known and four novel species, as well as the discovery of the ...

وصف الملف: application/pdf

العلاقة: Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; Mycologia; Akhmetova, G. K., Knapp, D. G., Özer, G., O’Donnell, K., Laraba, I., Kiyas, A., . & Molnár, O. (2023). Multilocus molecular phylogenetic-led discovery and formal recognition of four novel root-colonizing Fusarium species from northern Kazakhstan and the phylogenetically divergent Fusarium steppicola lineage. Mycologia, 115(1), 16-31.; PUBMED ID: 36441982; WOS:000891876500001; http://dx.doi.org/10.1080/00275514.2022.2119761Test; https://hdl.handle.net/20.500.12491/11742Test; 115; 16; 31

الإتاحة: https://doi.org/20.500.12491/11742Test
https://doi.org/10.1080/00275514.2022.2119761Test
https://hdl.handle.net/20.500.12491/11742Test

المؤلفون: Epstein, Lynn, Kaur, Sukhwinder, Chang, Peter L, Carrasquilla-Garcia, Noelia, Lyu, Guiyun, Cook, Douglas R, Subbarao, Krishna V, O'Donnell, Kerry

المصدر: Phytopathology. 107(4)

مصطلحات موضوعية: Fusarium, Sequence Analysis, DNA, Evolution, Molecular, Virulence, Plant Diseases, California, Genetic Variation, High-Throughput Nucleotide Sequencing, Apium, Apium graveolens, Fusarium yellows, phylogeny, Genetics, Plant Biology & Botany, Microbiology, Plant Biology, Crop and Pasture Production

الوصف: Fusarium oxysporum species complex (FOSC) isolates were obtained from celery with symptoms of Fusarium yellows between 1993 and 2013 primarily in California. Virulence tests and a two-gene dataset from 174 isolates indicated that virulent isolates collected before 2013 were a highly clonal population of F. oxysporum f. sp. apii race 2. In 2013, new highly virulent clonal isolates, designated race 4, were discovered in production fields in Camarillo, California. Long-read Illumina data were used to analyze 16 isolates: six race 2, one of each from races 1, 3, and 4, and seven genetically diverse FOSC that were isolated from symptomatic celery but are nonpathogenic on this host. Analyses of a 10-gene dataset comprising 38 kb indicated that F. oxysporum f. sp. apii is polyphyletic; race 2 is nested within clade 3, whereas the evolutionary origins of races 1, 3, and 4 are within clade 2. Based on 6,898 single nucleotide polymorphisms from the core FOSC genome, race 3 and the new highly virulent race 4 are highly similar with Nei's Da = 0.0019, suggesting that F. oxysporum f. sp. apii race 4 evolved from race 3. Next generation sequences were used to develop PCR primers that allow rapid diagnosis of races 2 and 4 in planta.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/5z73x0mfTest

المؤلفون: Spatafora, Joseph W, Chang, Ying, Benny, Gerald L, Lazarus, Katy, Smith, Matthew E, Berbee, Mary L, Bonito, Gregory, Corradi, Nicolas, Grigoriev, Igor, Gryganskyi, Andrii, James, Timothy Y, O'Donnell, Kerry, Roberson, Robert W, Taylor, Thomas N, Uehling, Jessie, Vilgalys, Rytas, White, Merlin M, Stajich, Jason E

المصدر: Mycologia. 108(5)

مصطلحات موضوعية: Microbiology, Biological Sciences, Ecology, Evolutionary Biology, Genetics, Fungi, Genome, Fungal, Phylogeny, Entomophthoromycotina, fungi, Glomeromycotina, Kickellomycotina, Mortierellomycotina, Mucoromycota, Mucoromycotina, paraphyly, systematics, Zoopagomycota Zoopagomycotina, Plant Biology, Mycology & Parasitology, Evolutionary biology, Plant biology

الوصف: Zygomycete fungi were classified as a single phylum, Zygomycota, based on sexual reproduction by zygospores, frequent asexual reproduction by sporangia, absence of multicellular sporocarps, and production of coenocytic hyphae, all with some exceptions. Molecular phylogenies based on one or a few genes did not support the monophyly of the phylum, however, and the phylum was subsequently abandoned. Here we present phylogenetic analyses of a genome-scale data set for 46 taxa, including 25 zygomycetes and 192 proteins, and we demonstrate that zygomycetes comprise two major clades that form a paraphyletic grade. A formal phylogenetic classification is proposed herein and includes two phyla, six subphyla, four classes and 16 orders. On the basis of these results, the phyla Mucoromycota and Zoopagomycota are circumscribed. Zoopagomycota comprises Entomophtoromycotina, Kickxellomycotina and Zoopagomycotina; it constitutes the earliest diverging lineage of zygomycetes and contains species that are primarily parasites and pathogens of small animals (e.g. amoeba, insects, etc.) and other fungi, i.e. mycoparasites. Mucoromycota comprises Glomeromycotina, Mortierellomycotina, and Mucoromycotina and is sister to Dikarya. It is the more derived clade of zygomycetes and mainly consists of mycorrhizal fungi, root endophytes, and decomposers of plant material. Evolution of trophic modes, morphology, and analysis of genome-scale data are discussed.

الوصول الحر: https://escholarship.org/uc/item/0648s6q4Test

المؤلفون: Torres-Cruz, Terry J., Whitaker, Briana K., Proctor, Robert H., Broders, Kirk, Laraba, Imane, Kim, Hye-Seon, Brown, Daren W., O'Donnell, Kerry, Estrada-Rodriguez, Tania L., Lee, Yong-Hwan, Cheong, Kyeongchae, Wallace, Emma C., McGee, Chyanna T., Kang, Seogchan, Geiser, David M.

المساهمون: Lee, Yong-Hwan, Cheong, Kyeongchae

الوصف: Species within Fusarium are of global agricultural, medical, and food/feed safety concern and have been extensively characterized. However, accurate identification of species is challenging and usually requires DNA sequence data FUSARIUM-ID (http://isoiate.fusariumdb.org/blastTest. php) is a publicly available database designed to support the identification of Fusarium species using sequences of multiple phylogenetically informative loci, especially the highly informative similar to 680-bp 5' portion of the translation elongation factor 1-alpha (TEF1) gene that has been adopted as the primary barcoding locus in the genus. However, FUSARIUM-ID v. 1.0 and 2.0 had several limitations, including inconsistent metadata annotation for the archived sequences and poor representation of some species complexes and marker loci. Here, we present FUSARIUM-ID v.3.0, which provides the following improvements: (i) additional and updated annotation of metadata for isolates associated with each sequence, (ii) expanded taxon representation in the TEF1 sequence database, (iii) availability of the sequence database as a downloadable file to enable local BLAST queries, and (iv) a tutorial file for users to perform local BLAST searches using either freely available software, such as SequenceServer, BLAST+ executable in the command line, and Galaxy, or the proprietary Geneious software. FUSARIUM-ID will be updated on a regular basis by archiving sequences of TEF1 and other loci from newly identified species and greater in-depth sampling of currently recognized species. ; N ; 1

العلاقة: Plant Disease, Vol.106 No.6, pp.1610-1616; https://hdl.handle.net/10371/185218Test; 000793049000003; 2-s2.0-85131271003; 164182

الإتاحة: https://hdl.handle.net/10371/185218Test