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1دورية أكاديمية
المؤلفون: Nunes, Valéria Sutti, Ilha, Angela de Oliveira Godoy, Ferreira, Guilherme da Silva, Bombo, Renata de Paula Assis, Afonso, Milessa Silva, Lavrador, Maria Silvia Ferrari, Machado, Roberta Marcondes, Nakandakare, Edna Regina, Quintão, Eder Carlos Rocha, Lottenberg, Ana Maria
المصدر: Clinics. January 2022 77
مصطلحات موضوعية: Cholesterol, Lathosterol, Phytosterols, Diet
الوصف: Objectives Because the plasma campesterol/cholesterol ratio does not differ between groups that absorb different amounts of cholesterol, the authors investigated whether the plasma Phytosterols (PS) relate to the body's cholesterol synthesis rate measured as non-cholesterol sterol precursors (lathosterol). Method The authors studied 38 non-obese volunteers (58±12 years; Low-Density Lipoprotein Cholesterol ‒ LDL-C ≥ 130 mg/dL) randomly assigned to consume 400 mL/day of soy milk (Control phase) or soy milk + PS (1.6 g/day) for four weeks in a double-blind, cross-over study. PS and lathosterol were measured in plasma by gas chromatography coupled to mass spectrophotometry. Results PS treatment reduced plasma total cholesterol concentration (-5.5%, p < 0.001), LDL-C (-7.6%, p < 0.001), triglycerides (-13.6%, p < 0.0085), and apolipoprotein B (apo B) (-6.3%, p < 0.008), without changing high density lipoprotein cholesterol (HDL-C concentration), but plasma lathosterol, campesterol and sitosterol expressed per plasma cholesterol increased. Conclusions The lathosterol-to-cholesterol plasma ratio predicted the plasma cholesterol response to PS feeding. The highest plasma lathosterol concentration during the control phase was associated with a lack of response of plasma cholesterol during the PS treatment period. Consequently, cholesterol synthesis in non-responders to dietary PS being elevated in the control phase indicates these cases resist to further synthesis rise, whereas responders to dietary PS, having in the control phase synthesis values lower than non-responders, expand synthesis on alimentary PS. Responders absorb more PS than non-responders, likely resulting from responders delivering into the intestinal lumen less endogenous cholesterol than non-responders do, thus facilitating greater intestinal absorption of PS shown as increased plasma PS concentration.
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2دورية أكاديمية
المؤلفون: Parra, Eliane Soler, Zago, Vanessa Helena de Souza, Panzoldo, Natália Baratella, Alexandre, Fernanda, Vendrame, Felipe, Virginio, Vitor Wilson de Moura, Vieira, Isabela Calanca, Rodrigues, Marcelo Scarpari Dutra, Cassol, Angela de Souza, D'Alexandri, Fabio, Castanho, Vera Sylvia, Nakamura, Rui, Baracat, Jamal, Danelon, Mirian Regina Gardin, Pozeti, Ronise Carla Sass, Facin, Ana Cândida Coutinho, Honorio, Hilda Maria da Silva, Mesquita, Simone de Cássia Dias, Silva, Marcio Jorge da, Lot, Marielza Crosgnac Feres, Nunes, Valéria Sutti, Leança, Camila Canteiro, Nakandakare, Edna Regina, Quintão, Eder Carlos Rocha, Faria, Eliana Cotta de
المصدر: Jornal Brasileiro de Patologia e Medicina Laboratorial. February 2013 49(1)
مصطلحات موضوعية: health institutes, HDL-C, data collection
الوصف: INTRODUCTION: The development of research for diagnosis, prevention and treatment of atherosclerotic cardiovascular disease is of utmost importance due to the fact that it is the main cause of morbidity and mortality in Brazil. OBJECTIVE: To demonstrate the phases of the selection process for candidates with the aim to develop a clinical-laboratorial database of hyper alpha lipoproteinemic patients (hyper A) - high density lipoprotein cholesterol (HDL-C) ≥ 68 mg/dl) and hypo alpha lipoproteinemic patients (hypo A) - HDL-C < 39 mg/dl. MATERIAL AND METHODS: The volunteers were contacted after selection of lipid profiles from individuals treated at the Sistema Único de Saúde (SUS), Campinas-SP and neighboring area. Afterwards, the selected patients went through blood collection, clinical examinations and answered questionnaires on dietary frequency and physical activity. After this preliminary evaluation, some individuals were convened to another blood collection and, subsequently, were submitted to an ultrasonographic exam of the carotid arteries. RESULTS: Only 0.6% and 0.3% from 598,288 lipid profiles were selected for hyper A and hypo A groups, respectively, including gender disparity. Lack of effective questionnaires (75%), missing calls (60%) and non-inclusion were the major hindrances in the construction of this database. DISCUSSION: The difficulties to obtain eligible candidates were also due to the low prevalence of both groups hypo A and hyper A and the high prevalence of pathologies that contribute to non-genetic variations of HDL-C. CONCLUSION: In spite of the obstacles in the development of this database, this study brought about several scientific publications. Furthermore, the development of molecular analyzes and functionality will shortly generate other findings, contributing to the diagnosis and follow-up of HDL dyslipidemias.
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3دورية أكاديمية
المؤلفون: Sawada, Maria Isabela Bloise Alves Caldas, de Fátima Mello Santana, Monique, Reis, Mozania, de Assis, Sayonara Ivana Santos, Pereira, Lucas Alves, Santos, Danielle Ribeiro, Nunes, Valéria Sutti, Correa-Giannella, Maria Lucia Cardillo, Gebrim, Luiz Henrique, Passarelli, Marisa
المساهمون: Fundação de Amparo à Pesquisa do Estado de São Paulo
المصدر: Scientific Reports ; volume 13, issue 1 ; ISSN 2045-2322
مصطلحات موضوعية: Multidisciplinary
الوصف: The association between plasma lipids and breast cancer (BC) has been extensively explored although results are still conflicting especially regarding the relationship with high-density lipoprotein cholesterol (HDLc) levels. HDL mediates cholesterol and oxysterol removal from cells limiting sterols necessary for tumor growth, inflammation, and metastasis and this may not be reflected by measuring HDLc. We addressed recently diagnosed, treatment-naïve BC women (n = 163), classified according to molecular types of tumors and clinical stages of the disease, in comparison to control women (CTR; n = 150) regarding plasma lipids and lipoproteins, HDL functionality and composition in lipids, oxysterols, and apo A-I. HDL was isolated by plasma discontinuous density gradient ultracentrifugation. Lipids (total cholesterol, TC; triglycerides, TG; and phospholipids, PL) were determined by enzymatic assays, apo A-I by immunoturbidimetry, and oxysterols (27, 25, and 24-hydroxycholesterol), by gas chromatography coupled with mass spectrometry. HDL-mediated cell cholesterol removal was determined in macrophages previously overloaded with cholesterol and 14 C-cholesterol. Lipid profile was similar between CTR and BC groups after adjustment per age. In the BC group, lower concentrations of TC (84%), TG (93%), PL (89%), and 27-hydroxicholesterol (61%) were observed in HDL, although the lipoprotein ability in removing cell cholesterol was similar to HDL from CRT. Triple-negative (TN) BC cases presented higher levels of TC, TG, apoB, and non-HDLc when compared to other molecular types. Impaired HDL functionality was observed in more advanced BC cases (stages III and IV), as cholesterol efflux was around 28% lower as compared to stages I and II. The altered lipid profile in TN cases may contribute to channeling lipids to tumor development in a hystotype with a more aggressive clinical history. Moreover, findings reinforce the dissociation between plasma levels of HDLc and HDL functionality in determining BC outcomes.
الإتاحة: https://doi.org/10.1038/s41598-023-35764-7Test
https://www.nature.com/articles/s41598-023-35764-7.pdfTest
https://www.nature.com/articles/s41598-023-35764-7Test -
4دورية أكاديمية
المؤلفون: Nakutis, Fernanda Serafim, Nishitokukado, Iêda, dos Santos, Fabiana Maria, Ortiz-Agostinho, Carmen Lucia, de Alencar, Daniel Teixeira, Achtschin, Cassiana Ganem, Nunes, Valeria Sutti, Leite, André Zonetti Arruda, Sipahi, Aytan Miranda
المساهمون: FAPESP
المصدر: Clinics ; volume 78, page 100305 ; ISSN 1807-5932
مصطلحات موضوعية: General Medicine
الإتاحة: https://doi.org/10.1016/j.clinsp.2023.100305Test
https://api.elsevier.com/content/article/PII:S1807593223001412?httpAccept=text/xmlTest
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5دورية أكاديمية
المؤلفون: Alves, Renato Jorge, Nunes, Valéria Sutti, Junior, Ney Carter do Carmo Borges, Nakandakare, Edna Regina, Quintão, Eder Carlos Rocha
المساهمون: Universidade de São Paulo
المصدر: Journal of Clinical Lipidology ; ISSN 1933-2874
الإتاحة: https://doi.org/10.1016/j.jacl.2024.04.128Test
https://api.elsevier.com/content/article/PII:S1933287424001764?httpAccept=text/xmlTest
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6دورية أكاديمية
المؤلفون: França, Victor, Gomes, Érica Ivana Lázaro, Campos, Edite Vieira Silva de, Zago, Vanessa Helena de Souza, Nunes, Valéria Sutti, Faria, Eliana Cotta de
المصدر: Clinics; v. 77 (2022); 100107 ; Clinics; Vol. 77 (2022); 100107 ; 1980-5322 ; 1807-5932
مصطلحات موضوعية: Lipoprotein (a), Carotid atherosclerosis, Carotid plaques
الوصف: Background: This study aimed to evaluate the associations between Lipoprotein (a) ‒ Lp(a) levels and carotid Intima-Media Thickness (cIMT) and with carotid plaques in healthy subjects because of previous contradictory data. Methods: A total of317healthy normolipidemic subjects (20‒77years old) were selected. The cIMT and atherosclerotic plaques were determined by B-mode ultrasonography. Mann-Whitney tests were performed to compare the groups according to Lp(a) levels and to explore the associations between Lp(a), carotid plaques, and cIMT, logistic and linear regression analyses were performed. Results: Studied population (51%females, median age43years old) presented carotid plaques and cIMT≥ 0.9mm in23% and18% of the participants, respectively. The group with Lp(a) levels> 30mg/dL presented significantly higher age and atherosclerotic plaques. Indeed, multivariate linear regression analysis showed a significant association between Lp(a), age, and race. On the other hand, logistic regression analysis demonstrated that the subjects with Lp(a)> 30mg/dL have a significantly high risk of carotid plaques. Conclusion: The data from the present study indicate that Lp(a) levels above30mg/dL contribute to the development of carotid plaques even in apparently healthy participants.
وصف الملف: application/pdf
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7دورية أكاديمية
المؤلفون: Fonseca, Fernanda Andrade Macaferri da, Espósito, Aline Paulino, Silva, Maria Helena Baptista Nunes da, Nunes, Valéria Sutti, Cazita, Patricia Miralda, Ferreira, Guilherme Silva, Ceccon, Maria Esther Jurfest Rivero, Carvalho, Werther Brunow de, Carneiro-Sampaio, Magda, Palmeira, Patricia
المصدر: Clinics; v. 77 (2022); 100111 ; Clinics; Vol. 77 (2022); 100111 ; 1980-5322 ; 1807-5932
مصطلحات موضوعية: Late-onset neonatal sepsis, Septic shock, Lipoproteins, Monocyte-to-HDL ratio
الوصف: Background: The association between lipoprotein levels and late-onset neonatal sepsis has shown controversial results. The aims are to assess lipid profile, cytokines, and Monocyte-to-HDL (M/H) ratio as diagnostic and prognostic markers for late-onset neonatal sepsis. Methods: This prospective study included49septic neonates and 17controls. Cholesterol (CT), Triglyceride (TG), Very-Low-Density (VLDLc), Low-Density (LDLc), and High-Density Lipoproteins (HDLc) were measured at admission (D0) and on days3,7 and10 to evaluate septic shock outcomes. Cytokines and monocytes were evaluated by flow cytometry. Results: Septic newborns showed higher IL-6 and IL-8 at D0 and CT levels on D7 and on D10, which also presented higher TG, VLDLc and non-HDL cholesterol concentrations than controls. The septic shock group (n = 22) revealed a higher number of male subjects, CRP, IL-6, IL-8 and IL-10 levels, while lower TG, HDLc, monocyte numbers and M/H ratio at admission compared to the non-shock group (n = 27). M/H ratio and non-HDL cholesterol on D0 were risk factors for septic shock (OR = 0.70, 0.49‒0.99; OR = 0.96, 0.92‒0.99, respectively). Decreasing levels from D0 to D3 of CT (OR = 0.96, 0.93‒0.99), VLDLc (OR = 0.91, 0.85‒0.98), and non-HDL cholesterol (OR = 0.92, 0.87‒0.98) were also predictors of septic shock. Conclusions: Lower M/H ratios and non-HDL cholesterol at admission and decreasing levels of cholesterol, VLDLc and non-HDL cholesterol during a hospital stay are associated with the development of septic shock in newborns with late-onset neonatal sepsis.
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8دورية أكاديمية
المؤلفون: Santana, Kelly Gomes, Righetti, Renato Fraga, Breda, Cristiane Naffah de Souza, Dominguez-Amorocho, Omar Alberto, Ramalho, Theresa, Dantas, Francisca Elda B., Nunes, Valeria Sutti, Tiberio, Iolanda de Fatima Lopes Calvo, Soriano, Francisco Garcia, Camara, Niels O.S., Quintao, Eder Carlos Rocha, Cazita, Patricia M.
المساهمون: Division of Infectious Diseases and Immunology, Department of Medicine
المصدر: Frontiers in immunology ; 12 ; 684076
مصطلحات موضوعية: arginase 1, cholesterol ester transfer protein, chronic obstructive pulmonary disease, inflammation, interleukin-10, macrophage—cell, pulmonary emphysema, Immunology and Infectious Disease, Respiratory Tract Diseases
الوصف: Cholesterol-ester transfer protein (CETP) plays a role in atherosclerosis, the inflammatory response to endotoxemia and in experimental and human sepsis. Functional alterations in lipoprotein (LP) metabolism and immune cell populations, including macrophages, occur during sepsis and may be related to comorbidities such as chronic obstructive pulmonary disease (COPD). Macrophages are significantly associated with pulmonary emphysema, and depending on the microenvironment, might exhibit an M1 or M2 phenotype. Macrophages derived from the peritoneum and bone marrow reveal CETP that contributes to its plasma concentration. Here, we evaluated the role of CETP in macrophage polarization and elastase-induced pulmonary emphysema (ELA) in human CETP-expressing transgenic (huCETP) (line 5203, C57BL6/J background) male mice and compared it to their wild type littermates. We showed that bone marrow-derived macrophages from huCETP mice reduce polarization toward the M1 phenotype, but with increased IL-10. Compared to WT, huCETP mice exposed to elastase showed worsened lung function with an increased mean linear intercept (Lm), reflecting airspace enlargement resulting from parenchymal destruction with increased expression of arginase-1 and IL-10, which are M2 markers. The cytokine profile revealed increased IL-6 in plasma and TNF, and IL-10 in bronchoalveolar lavage (BAL), corroborating with the lung immunohistochemistry in the huCETP-ELA group compared to WT-ELA. Elastase treatment in the huCETP group increased VLDL-C and reduced HDL-C. Elastase-induced pulmonary emphysema in huCETP mice promotes lung M2-like phenotype with a deleterious effect in experimental COPD, corroborating the in vitro result in which CETP promoted M2 macrophage polarization. Our results suggest that CETP is associated with inflammatory response and influences the role of macrophages in COPD.
العلاقة: Link to Article in PubMed; Santana KG, Righetti RF, Breda CNS, Domínguez-Amorocho OA, Ramalho T, Dantas FEB, Nunes VS, Tibério IFLC, Soriano FG, Câmara NOS, Quintão ECR, Cazita PM. Cholesterol-Ester Transfer Protein Alters M1 and M2 Macrophage Polarization and Worsens Experimental Elastase-Induced Pulmonary Emphysema. Front Immunol. 2021 Jul 21;12:684076. doi:10.3389/fimmu.2021.684076. PMID: 34367144; PMCID: PMC8334866. Link to article on publisher's site; 1664-3224 (Linking); http://hdl.handle.net/20.500.14038/42032Test; https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5869&context=oapubs&unstamped=1Test; https://escholarship.umassmed.edu/oapubs/4836Test; oapubs/4836
الإتاحة: https://doi.org/10.3389/fimmu.2021.684076Test
https://doi.org/20.500.14038/42032Test
https://hdl.handle.net/20.500.14038/42032Test
https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=5869&context=oapubs&unstamped=1Test
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9دورية أكاديمية
المؤلفون: Nunes, Valéria Sutti, da Silva, Eliton Juniro, Ferreira, Guilherme da Silva, Assis, Sayonara Ivana Santos de, Cazita, Patrícia Miralda, Nakandakare, Edna Regina, Zago, Vanessa Helena de Souza, de Faria, Eliana Cotta, Quintão, Eder Carlos Rocha
المساهمون: Fundação de Amparo à Pesquisa do Estado de São Paulo
المصدر: Frontiers in Nutrition ; volume 9 ; ISSN 2296-861X
مصطلحات موضوعية: Nutrition and Dietetics, Endocrinology, Diabetes and Metabolism, Food Science
الوصف: Non-cholesterol sterols are transported in plasma lipoproteins and are consequently important in cholesterol metabolism. We investigated the distribution of non-cholesterol sterol precursors of cholesterol synthesis (NCSPCS), oxysterols, and phytosterols in lipoproteins of healthy subjects differing according to HDL-Cholesterol (HDL-C) plasma levels. Elevated NCSPCS (desmosterol, lathosterol) in the High HDL group suggests that HDL exports these sterols from cells, but not the cholesterol metabolite 24-OHC which was higher in the Low HDL group than in the High HDL group. 27-hydroxycholesterol (27OH-C) plasma levels did not differ between groups. Percentage of NCSPCS and phytosterols predominates in LDL, but did not differ between groups. Thirty percent of desmosterol and lathosterol are present in HDL, with the High HDL group carrying higher percentage of these sterols. A high percentage of campesterol and sitosterol in HDL suggests that phytosterols are absorbed by enterocytes, and that HDL could be a marker of the ABCA1/ApoA1 intestinal activity.
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10دورية أكاديمية
المؤلفون: da Fonseca, Fernanda Andrade Macaferri, Espósito, Aline Paulino, da Silva, Maria Helena Baptista Nunes, Nunes, Valéria Sutti, Cazita, Patricia Miralda, Ferreira, Guilherme Silva, Ceccon, Maria Esther Jurfest Rivero, de Carvalho, Werther Brunow, Carneiro-Sampaio, Magda, Palmeira, Patricia
المصدر: Clinics ; volume 77, page 100111 ; ISSN 1807-5932
مصطلحات موضوعية: General Medicine
الإتاحة: https://doi.org/10.1016/j.clinsp.2022.100111Test
https://api.elsevier.com/content/article/PII:S1807593222033129?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S1807593222033129?httpAccept=text/plainTest
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