المؤلفون: Shinta Saito, Noritaka Adachi

المصدر: Nature Communications, Vol 15, Iss 1, Pp 1-13 (2024)

مصطلحات موضوعية: Science

الوصف: Abstract Homology-dependent targeted DNA integration, generally referred to as gene targeting, provides a powerful tool for precise genome modification; however, its fundamental mechanisms remain poorly understood in human cells. Here we reveal a noncanonical gene targeting mechanism that does not rely on the homologous recombination (HR) protein Rad51. This mechanism is suppressed by Rad52 inhibition, suggesting the involvement of single-strand annealing (SSA). The SSA-mediated gene targeting becomes prominent when DSB repair by HR or end-joining pathways is defective and does not require isogenic DNA, permitting 5% sequence divergence. Intriguingly, loss of Msh2, loss of BLM, and induction of a target-site DNA break all significantly and synergistically enhance SSA-mediated targeted integration. Most notably, SSA-mediated integration is cell cycle-independent, occurring in the G1 phase as well. Our findings provide unequivocal evidence for Rad51-independent targeted integration and unveil multiple mechanisms to regulate SSA-mediated targeted as well as random integration.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2041-1723Test

الوصول الحر: https://doaj.org/article/0d632fe34ba2428fb23eda174aad8ea7Test

المؤلفون: Tadaaki Nakajima, Akihiro Imai, Chihiro Ishii, Kota Tsuruyama, Risa Yamanaka, Yasuhiro Tomooka, Shinta Saito, Noritaka Adachi, Satomi Kohno, Tomomi Sato

المصدر: FEBS Open Bio, Vol 14, Iss 1, Pp 37-50 (2024)

مصطلحات موضوعية: epithelialization, fate determination, Müllerian duct, TGFβ signaling, Wolffian duct, Biology (General), QH301-705.5

الوصف: Male and female reproductive tracts develop from anterior intermediate mesoderm with similar differentiation processes. The anterior intermediate mesoderm develops into the mesonephros, and the Wolffian duct initiates by epithelialization in the mesonephros. The Müllerian duct invaginates from the coelomic epithelium of the cranial mesonephros for ductal formation and is then regionalized into proximal to caudal female reproductive tracts. In this study, we focused on the epithelialization of the Wolffian duct, initiation of the Müllerian duct, and the regionalization step of the Müllerian ducts as a continuous process. By using intermediate mesodermal cells from mouse pluripotent stem cells, we identified that inhibition of SMAD2/3 signaling might be involved in the differentiation into mesenchymal cells, after which mesonephric cells might be then epithelialized during differentiation of the Wolffian duct. Aggregation of coelomic epithelial cells might be related to initiation of the Müllerian duct. Transcriptomic analysis predicted that consensus sequences of SMAD3/4 were enriched among highly expressed genes in the proximal Müllerian duct. SMAD2/3 signaling to regulate differentiation of the Wolffian duct was continuously activated in the proximal Müllerian duct and was involved in proximal and oviductal regionalization. Therefore, SMAD2/3 signaling may be finely tuned to regulate differentiation from initiation to regionalization steps.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2211-5463Test

الوصول الحر: https://doaj.org/article/5d2823eb949b4704816bf72b9265cfcaTest

المؤلفون: Takayuki Onai, Noritaka Adachi, Hidetoshi Urakubo, Fumiaki Sugahara, Toshihiro Aramaki, Mami Matsumoto, Nobuhiko Ohno

المصدر: iScience, Vol 26, Iss 12, Pp 108338- (2023)

مصطلحات موضوعية: Zoology, Evolutionary biology, Evolutionary developmental biology, Science

الوصف: Summary: The cranial muscle is a critical component in the vertebrate head for a predatory lifestyle. However, its evolutionary origin and possible segmental nature during embryogenesis have been controversial. In jawed vertebrates, the presence of pre-otic segments similar to trunk somites has been claimed based on developmental observations. However, evaluating such arguments has been hampered by the paucity of research on jawless vertebrates. Here, we discovered different cellular arrangements in the head mesoderm in lamprey embryos (Lethenteron camtschaticum) using serial block-face scanning electron and laser scanning microscopies. These cell populations were morphologically and molecularly different from somites. Furthermore, genetic comparison among deuterostomes revealed that mesodermal gene expression domains were segregated antero-posteriorly in vertebrates, whereas such segregation was not recognized in invertebrate deuterostome embryos. These findings indicate that the vertebrate head mesoderm evolved from the anteroposterior repatterning of an ancient mesoderm and developmentally diversified before the split of jawless and jawed vertebrates.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S258900422302415XTest; https://doaj.org/toc/2589-0042Test

الوصول الحر: https://doaj.org/article/27de3e14778a4e829162f97a0b694c92Test

المؤلفون: Wataru Takagi, Fumiaki Sugahara, Shinnosuke Higuchi, Rie Kusakabe, Juan Pascual-Anaya, Iori Sato, Yasuhiro Oisi, Nobuhiro Ogawa, Hiroshi Miyanishi, Noritaka Adachi, Susumu Hyodo, Shigeru Kuratani

المصدر: BMC Biology, Vol 20, Iss 1, Pp 1-10 (2022)

مصطلحات موضوعية: Endostyle, Thyroid gland, Cyclostomes, Hagfish, Lamprey, Evolution, Biology (General), QH301-705.5

الوصف: Abstract Background The endostyle is an epithelial exocrine gland found in non-vertebrate chordates (amphioxi and tunicates) and the larvae of modern lampreys. It is generally considered to be an evolutionary precursor of the thyroid gland of vertebrates. Transformation of the endostyle into the thyroid gland during the metamorphosis of lampreys is thus deemed to be a recapitulation of a past event in vertebrate evolution. In 1906, Stockard reported that the thyroid gland in hagfish, the sister cyclostome group of lampreys, develops through an endostyle-like primordium, strongly supporting the plesiomorphy of the lamprey endostyle. However, the findings in hagfish thyroid development were solely based on this single study, and these have not been confirmed by modern molecular, genetic, and morphological data pertaining to hagfish thyroid development over the last century. Results Here, we showed that the thyroid gland of hagfish undergoes direct development from the ventrorostral pharyngeal endoderm, where the previously described endostyle-like primordium was not found. The developmental pattern of the hagfish thyroid, including histological features and regulatory gene expression profiles, closely resembles that found in modern jawed vertebrates (gnathostomes). Meanwhile, as opposed to gnathostomes but similar to non-vertebrate chordates, lamprey and hagfish share a broad expression domain of Nkx2-1/2-4, a key regulatory gene, in the pharyngeal epithelium during early developmental stages. Conclusions Based on the direct development of the thyroid gland both in hagfish and gnathostomes, and the shared expression profile of thyroid-related transcription factors in the cyclostomes, we challenge the plesiomorphic status of the lamprey endostyle and propose an alternative hypothesis where the lamprey endostyle could be obtained secondarily in crown lampreys.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1741-7007Test

الوصول الحر: https://doaj.org/article/45d56dbca5244a99a6b79a5740ebb360Test

المؤلفون: Gabriella Lania, Monica Franzese, Noritaka Adachi, Marchesa Bilio, Gemma Flore, Annalaura Russo, Erika D'Agostino, Claudia Angelini, Robert G. Kelly, Antonio Baldini

المصدر: Disease Models & Mechanisms, Vol 15, Iss 9 (2022)

مصطلحات موضوعية: tbx1, digeorge syndrome, pharyngeal apparatus, cardiopharyngeal mesoderm, phenotypic rescue, Medicine, Pathology, RB1-214

وصف الملف: electronic resource

العلاقة: http://dmm.biologists.org/content/15/9/dmm049415Test; https://doaj.org/toc/1754-8403Test; https://doaj.org/toc/1754-8411Test

الوصول الحر: https://doaj.org/article/e14444d7bc5a479dafb4d2f3e60dd9c8Test

المؤلفون: Yuduki Someya, Sakine Kobayashi, Kazuya Toriumi, Shigeki Takeda, Noritaka Adachi, Aya Kurosawa

المصدر: Genes, Vol 14, Iss 2, p 420 (2023)

مصطلحات موضوعية: non-homologous end-joining, DNA double-strand break, DNA single-strand break, natural antioxidant, antitumor effect, Genetics, QH426-470

الوصف: Natural antioxidants derived from plants exert various physiological effects, including antitumor effects. However, the molecular mechanisms of each natural antioxidant have not yet been fully elucidated. Identifying the targets of natural antioxidants with antitumor properties in vitro is costly and time-consuming, and the results thus obtained may not reliably reflect in vivo conditions. Therefore, to enhance understanding regarding the antitumor effects of natural antioxidants, we focused on DNA, one of the targets of anticancer drugs, and evaluated whether antioxidants, e.g., sulforaphane, resveratrol, quercetin, kaempferol, and genistein, which exert antitumor effects, induce DNA damage using gene-knockout cell lines derived from human Nalm-6 and HeLa cells pretreated with the DNA-dependent protein kinase inhibitor NU7026. Our results suggested that sulforaphane induces single-strand breaks or DNA strand crosslinks and that quercetin induces double-strand breaks. In contrast, resveratrol showed the ability to exert cytotoxic effects other than DNA damage. Our results also suggested that kaempferol and genistein induce DNA damage via unknown mechanisms. Taken together, the use of this evaluation system facilitates the analysis of the cytotoxic mechanisms of natural antioxidants.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2073-4425/14/2/420Test; https://doaj.org/toc/2073-4425Test

الوصول الحر: https://doaj.org/article/459042abda5f4d4d9df1c502bd3047b1Test

المؤلفون: Shunya Kuroda, Noritaka Adachi, Rie Kusakabe, Shigeru Kuratani

المصدر: Zoological Letters, Vol 7, Iss 1, Pp 1-11 (2021)

مصطلحات موضوعية: Head muscles, Head cavity, Head mesoderm, Extraocular muscles, Tendon, Zoology, QL1-991

الوصف: Abstract Vertebrate extraocular muscles (EOMs) function in eye movements. The EOMs of modern jawed vertebrates consist primarily of four recti and two oblique muscles innervated by three cranial nerves. The developmental mechanisms underlying the establishment of this complex and the evolutionarily conserved pattern of EOMs are unknown. Chondrichthyan early embryos develop three pairs of overt epithelial coeloms called head cavities (HCs) in the head mesoderm, and each HC is believed to differentiate into a discrete subset of EOMs. However, no direct evidence of these cell fates has been provided due to the technical difficulty of lineage tracing experiments in chondrichthyans. Here, we set up an in ovo manipulation system for embryos of the cloudy catshark Scyliorhinus torazame and labeled the epithelial cells of each HC with lipophilic fluorescent dyes. This experimental system allowed us to trace the cell lineage of EOMs with the highest degree of detail and reproducibility to date. We confirmed that the HCs are indeed primordia of EOMs but showed that the morphological pattern of shark EOMs is not solely dependent on the early pattern of the head mesoderm, which transiently appears as tripartite HCs along the simple anteroposterior axis. Moreover, we found that one of the HCs gives rise to tendon progenitor cells of the EOMs, which is an exceptional condition in our previous understanding of head muscles; the tendons associated with head muscles have generally been supposed to be derived from cranial neural crest (CNC) cells, another source of vertebrate head mesenchyme. Based on interspecies comparisons, the developmental environment is suggested to be significantly different between the two ends of the rectus muscles, and this difference is suggested to be evolutionarily conserved in jawed vertebrates. We propose that the mesenchymal interface (head mesoderm vs CNC) in the environment of developing EOM is required to determine the processes of the proximodistal axis of rectus components of EOMs.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2056-306XTest

الوصول الحر: https://doaj.org/article/d1b0736a984e448eaf12a0e0e132350fTest

المؤلفون: Rik Schrijvers, Jan De Rijck, Jonas Demeulemeester, Noritaka Adachi, Sofie Vets, Keshet Ronen, Frauke Christ, Frederic D Bushman, Zeger Debyser, Rik Gijsbers

المصدر: PLoS Pathogens, Vol 16, Iss 9, p e1008894 (2020)

مصطلحات موضوعية: Immunologic diseases. Allergy, RC581-607, Biology (General), QH301-705.5

الوصف: [This corrects the article DOI: 10.1371/journal.ppat.1002558.].

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1553-7366Test; https://doaj.org/toc/1553-7374Test

الوصول الحر: https://doaj.org/article/11964b88863148aab319d60d477e35bbTest

المؤلفون: Noritaka Adachi, Juan Pascual-Anaya, Tamami Hirai, Shinnosuke Higuchi, Shunya Kuroda, Shigeru Kuratani

المصدر: Zoological Letters, Vol 4, Iss 1, Pp 1-12 (2018)

مصطلحات موضوعية: Hepatocyte growth factor HGF, Receptor tyrosine kinase MET, Hypobranchial muscles, Limb/fin muscles, Migratory muscle precursor cells, Vertebrate evolution, Zoology, QL1-991

الوصف: Abstract Background The skeletal musculature of gnathostomes, which is derived from embryonic somites, consists of epaxial and hypaxial portions. Some hypaxial muscles, such as tongue and limb muscles, undergo de-epithelialization and migration during development. Delamination and migration of these myoblasts, or migratory muscle precursors (MMPs), is generally thought to be regulated by hepatocyte growth factor (HGF) and receptor tyrosine kinase (MET) signaling. However, the prevalence of this mechanism and the expression patterns of the genes involved in MMP development across different vertebrate species remain elusive. Results We performed a comparative analysis of Hgf and Met gene expression in several vertebrates, including mouse, chicken, dogfish (Scyliorhinus torazame), and lamprey (Lethenteron camtschaticum). While both Hgf and Met were expressed during development in the mouse tongue muscle, and in limb muscle formation in the mouse and chicken, we found no clear evidence for the involvement of HGF/MET signaling in MMP development in shark or lamprey embryos. Conclusions Our results indicate that the expressions and functions of both Hgf and Met genes do not represent shared features of vertebrate MMPs, suggesting a stepwise participation of HGF/MET signaling in MMP development during vertebrate evolution.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s40851-018-0094-yTest; https://doaj.org/toc/2056-306XTest

الوصول الحر: https://doaj.org/article/08166ab5052a4933bb63e6cd69ba8ef6Test

المؤلفون: Noritaka Adachi, Juan Pascual-Anaya, Tamami Hirai, Shinnosuke Higuchi, Shigeru Kuratani

المصدر: Zoological Letters, Vol 4, Iss 1, Pp 1-13 (2018)

مصطلحات موضوعية: Head–trunk interface, Hypobranchial muscles, Pericardium, Pharyngeal arch, Zoology, QL1-991

الوصف: Abstract Background The extant vertebrates include cyclostomes (lamprey and hagfish) and crown gnathostomes (jawed vertebrates), but there are various anatomical disparities between these two groups. Conspicuous in the gnathostomes is the neck, which occupies the interfacial domain between the head and trunk, including the occipital part of the cranium, the shoulder girdle, and the cucullaris and hypobranchial muscles (HBMs). Of these, HBMs originate from occipital somites to form the ventral pharyngeal and neck musculature in gnathostomes. Cyclostomes also have HBMs on the ventral pharynx, but lack the other neck elements, including the occipital region, the pectoral girdle, and cucullaris muscles. These anatomical differences raise questions about the evolution of the neck in vertebrates. Results In this study, we observed developing HBMs as a basis for comparison between the two groups and show that the arrangement of the head–trunk interface in gnathostomes is distinct from that of lampreys. Our comparative analyses reveal that, although HBM precursors initially pass through the lateral side of the pericardium in both groups, the relative positions of the pericardium withrespect to the pharyngeal arches differ between the two, resulting in diverse trajectories of HBMs in gnathostomes and lampreys. Conclusions We suggest that a heterotopic rearrangement of early embryonic components, including the pericardium and pharyngeal arches, may have played a fundamental role in establishing the gnathostome HBMs, which would also have served as the basis for neck formation in the jawed vertebrate lineage.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s40851-018-0087-xTest; https://doaj.org/toc/2056-306XTest

الوصول الحر: https://doaj.org/article/167ca167eff2474ea5fa8fad2cb3b48aTest