المؤلفون: Reyes-Goya, Claudia, Santana-Garrido, Álvaro, Aguilar-Espejo, Gema, Pérez-Camino, M Carmen, Mate, Alfonso, Vázquez, Carmen M

مصطلحات موضوعية: Acebuche, Aorta, Arterial hypertension, Endothelial dysfunction, NG-nitro-l-arginine methyl ester, Wild olive oil

الوصف: Despite numerous reports on the beneficial effects of olive oil in the cardiovascular context, very little is known about the olive tree's wild counterpart (Olea europaea, L. var. sylvestris), commonly known as acebuche (ACE) in Spain. The aim of this study was to analyse the possible beneficial effects of an extra virgin ACE oil on vascular function in a rodent model of arterial hypertension (AH) induced by NG-nitro-l-arginine methyl ester (L-NAME). Four experimental groups of male Wistar rats were studied: (1) normotensive rats (Control group); (2) normotensive rats fed a commercial diet supplemented with 15 % (w/w) ACE oil (Acebuche group); (3) rats made hypertensive following administration of L-NAME (L-NAME group); and (4) rats treated with L-NAME and simultaneously supplemented with 15 % ACE oil (LN + ACE group). All treatments were maintained for 12 weeks. Besides a significant blood pressure (BP)-lowering effect, the ACE oil-enriched diet counteracted the alterations found in aortas from hypertensive rats in terms of morphology and responsiveness to vasoactive mediators. In addition, a decrease in hypertension-related fibrotic and oxidative stress processes was observed in L-NAME-treated rats subjected to ACE oil supplement. Therefore, using a model of AH via nitric oxide depletion, here we demonstrate the beneficial effects of a wild olive oil based upon its vasodilator, antihypertensive, antioxidant, antihypertrophic and antifibrotic properties. We postulate that regular inclusion of ACE oil in the diet can alleviate the vascular remodelling and endothelial dysfunction processes typically found in AH, thus resulting in a significant reduction of BP.

وصف الملف: application/pdf

العلاقة: http://hdl.handle.net/10668/22569Test; PMC9530918; https://www.cambridge.org/core/services/aop-cambridge-core/content/view/B61D35D851370F30AA7BCC54A52581B9/S0007114522000034a.pdf/div-class-title-daily-consumption-of-wild-olive-acebuche-oil-reduces-blood-pressure-and-ameliorates-endothelial-dysfunction-and-vascular-remodelling-in-rats-withTest-ng-nitro-l-arginine-methyl-ester-induced-hypertension-div.pdf; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530918/pdfTest

الإتاحة: https://doi.org/10.1017/S0007114522000034Test
http://hdl.handle.net/10668/22569Test
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/B61D35D851370F30AA7BCC54A52581B9/S0007114522000034a.pdf/div-class-title-daily-consumption-of-wild-olive-acebuche-oil-reduces-blood-pressure-and-ameliorates-endothelial-dysfunction-and-vascular-remodelling-in-rats-withTest-ng-nitro-l-arginine-methyl-ester-induced-hypertension-div.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530918/pdfTest

المؤلفون: Ayesha Rahman Ahmed, Mahiba Ahmed, Senty Vun-Sang, Mohammad Iqbal

المصدر: Molecules; Volume 27; Issue 14; Pages: 4362

مصطلحات موضوعية: tissue injury, nitric oxide, glyceryl trinitrate, NG-nitro- l -arginine methyl ester, oxidative stress, organ toxicity

جغرافية الموضوع: agris

الوصف: Oxidative stress induced by well-known toxins including ferric nitrilotriacetate (Fe-NTA), carbon tetrachloride (CCl4) and thioacetamide (TAA) has been attributed to causing tissue injury in the liver and kidney. In this study, the effect of glyceryl trinitrate (GTN), a donor of nitric oxide and NG-nitroarginine methyl ester (l-NAME), a nitric oxide inhibitor on TAA-induced hepatic oxidative stress, GSH and GSH-dependent enzymes, serum transaminases and tumor promotion markers such as ornithine decarboxylase (ODC) activity and [3H]-thymidine incorporation in rats were examined. The animals were divided into seven groups consisting of six healthy rats per group. The six rats were injected intraperitoneally with TAA to evaluate its toxic effect, improvement in its toxic effect if any, or worsening in its toxic effect if any, when given in combination with GTN or l-NAME. The single necrogenic dose of TAA administration caused a significant change in the levels of both hepatic and serum enzymes such as glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx), γ-glutamyl transpeptidase (GGT), glucose 6-phosphate dehydrogenase (G6PD), alanine aminotransferase (AST) and aspartate aminotransferase (ALT). In addition, treatment with TAA also augmented malondialdehyde (MDA), ornithine decarboxylase (ODC) activity and [3H]-thymidine incorporation in rats liver. Concomitantly, TAA treatment depleted the levels of GSH. However, most of these changes were alleviated by the treatment of animals with GTN dose-dependently. The protective effect of GTN against TAA was also confirmed histopathologically. The present data confirmed our earlier findings with other oxidants including Fe-NTA and CCl4. The GTN showed no change whatsoever when administered alone, however when it was given along with TAA then it showed protection thereby contributing towards defending the role against oxidants-induced organ toxicity. Overall, GTN may contribute to protection against TAA-induced oxidative stress, toxicity, ...

وصف الملف: application/pdf

العلاقة: Green Chemistry; https://dx.doi.org/10.3390/molecules27144362Test

الإتاحة: https://doi.org/10.3390/molecules27144362Test

المؤلفون: Shuangyan Yang, Lili Song, Xiaofeng Shi, Na Zhao, Yaxin Ma

المصدر: Biomedicine & Pharmacotherapy, Vol 116, Iss , Pp 108969- (2019)

مصطلحات موضوعية: Pre-eclampsia, NG-nitro-L-arginine-methyl-ester, Aspirin, Quercetin, Therapeutics. Pharmacology, RM1-950

الوصف: As an inflammatory disease, pre-eclampsia is correlated with elevation of pro-inflammatory cytokines and maternal endothelial dysfunction. Aspirin plays an important role in the prevention and therapy of pre-eclampsia. Quercetin is a bioflavonoid which has anti-oxidant and reno-protective abilities. We aimed to figure out the effects of quercetin supplement to aspirin on the therapy against pre-eclampsia. Female pregnant Sprague-Dawley rats were divided into five groups according to the drug treatment. Aspirin [1.5 mg/kg body weight (BW)] or quercetin (2 mg/kg BW) treatment was administered from gestational day (GD) 4 to GD19. Rat model of pre-eclampsia was induced by NG-nitro-Larginine-methyl-ester (L-NAME). In pre-eclampsia rats induced by L-NAME, systolic blood pressures (SBP), proteinuria, malonyldialdehyde (MDA), and inflammatory cytokines levels were decreased by the treatment of quercetin supplement to aspirin. In the uterus, quercetin supplement to aspirin prevented the expression of VEGF and sFlt-1 mRNA. The treatment of quercetin supplement to aspirin rescued the declined survival rate and weight of pups caused by L-NAME-induced pre-eclampsia. Based on our study, compared with the treatment of aspirin alone, quercetin supplement to aspirin enhanced the therapeutic effects of aspirin on pre-eclampsia rats induced by L-NAME.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S0753332219306717Test; https://doaj.org/toc/0753-3322Test

الوصول الحر: https://doaj.org/article/022be38c835c4237870c92fbcaa1ca6fTest

المؤلفون: Esmaeil Farrokhi, Mohammad Naser Shafei, Abolfazl Khajavirad, Mahmoud Hosseini, Ali Reza Ebrahimzadeh Bideskan

المصدر: Iranian Journal of Medical Sciences, Vol 42, Iss 5, Pp 473-480 (2017)

مصطلحات موضوعية: Cuneiform nucleus, Nitroprusside, Nitric oxide, Blood pressure, NG-nitro-L-arginine methyl ester, Medicine (General), R5-920

الوصف: Background: The presence of nitric oxide (NO) in the cuneiform nucleus (CnF) has been previously shown. In this study, NG-nitro-L-arginine methyl ester (L-NAME) (an inhibitor of NO synthase), L-arginine (L-Arg) (a precursor of NO), and sodium nitroprusside (SNP) (a donor of NO) were microinjected into the CnF and cardiovascular responses were investigated. Methods: Seventy male rats were divided into 7 groups (n=10 each): 1) saline, 2 and 3) L-NAME (30 and 90 nmol), 4 and 5) L-Arg (20 and 60 nmol), and 6 and 7) SNP (9 and 27 nmol). After anesthesia, the femoral artery was cannulated and cardiovascular parameters were recorded using a PowerLab system. Time course changes in mean arterial pressure (ΔMAP) and heart rate (ΔHR) were calculated and compared with those in the control group (repeated measures ANOVA). Maximum ∆MAP and ∆HR were also compared with those in the control group (independent sample t test). Results: ∆MAP with both doses of L-NAME (30: P=0.026 and 90: P=0.007) and ∆HR with the higher dose (P=0.034) were significantly higher than those in the control group. Maximal ∆MAP with both doses (P

وصف الملف: electronic resource

العلاقة: http://ijms.sums.ac.ir/index.php/IJMS/article/view/3082Test; https://doaj.org/toc/0253-0716Test; https://doaj.org/toc/1735-3688Test

الوصول الحر: https://doaj.org/article/9d37bd787f3b44c0a84567e304047ed2Test

المؤلفون: Claudia Reyes-Goya, Álvaro Santana-Garrido, Gema Aguilar-Espejo, M. Carmen Pérez-Camino, Alfonso Mate, Carmen M. Vázquez

المساهمون: Junta de Andalucía

المصدر: Digital.CSIC. Repositorio Institucional del CSIC
instname

مصطلحات موضوعية: Arterial hypertension, Nutrition and Dietetics, Acebuche, Medicine (miscellaneous), Wild olive oil, Endothelial dysfunction, NG-nitro-l-arginine methyl ester, Aorta

الوصف: 14 Páginas.-- 6 Figuras.-- 3 Tablas
Despite numerous reports on the beneficial effects of olive oil in the cardiovascular context, very little is known about the olive tree's wild counterpart (Olea europaea, L. var. sylvestris), commonly known as acebuche (ACE) in Spain. The aim of this study was to analyse the possible beneficial effects of an extra virgin ACE oil on vascular function in a rodent model of arterial hypertension (AH) induced by NG-nitro-l-arginine methyl ester (L-NAME). Four experimental groups of male Wistar rats were studied: (1) normotensive rats (Control group); (2) normotensive rats fed a commercial diet supplemented with 15 % (w/w) ACE oil (Acebuche group); (3) rats made hypertensive following administration of L-NAME (L-NAME group); and (4) rats treated with L-NAME and simultaneously supplemented with 15 % ACE oil (LN + ACE group). All treatments were maintained for 12 weeks. Besides a significant blood pressure (BP)-lowering effect, the ACE oil-enriched diet counteracted the alterations found in aortas from hypertensive rats in terms of morphology and responsiveness to vasoactive mediators. In addition, a decrease in hypertension-related fibrotic and oxidative stress processes was observed in L-NAME-treated rats subjected to ACE oil supplement. Therefore, using a model of AH via nitric oxide depletion, here we demonstrate the beneficial effects of a wild olive oil based upon its vasodilator, antihypertensive, antioxidant, antihypertrophic and antifibrotic properties. We postulate that regular inclusion of ACE oil in the diet can alleviate the vascular remodelling and endothelial dysfunction processes typically found in AH, thus resulting in a significant reduction of BP.
We thank the Centro de Innovación, Tecnología e Innovación de la Universidad de Sevilla (CITIUS, Servicio de Biología, Servicio de Microscopía) for technical support. The authors are especially grateful to Alcazarín Reunidos FP, S.L. (Monda, Málaga, Spain) for providing the extra virgin wild olive oil (ACE oil) used in this study. This study was supported by Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía (2020/275; CTS-584); AS is recipient of an FPU predoctoral fellowship from Ministerio de Ciencia, Innovación y Universidades (FPU17/03465). C. R. G was supported by Ministerio de Ciencia e Innovación, Ayudas para la Promoción de Empleo Joven e Implantación de la Garantía Juvenil en I + D + i 2017–2020 (PEJ2018–004474-A). Study design and data management, C. R. G., A. M. and C. M. V.; data acquisition, C. R. G., Á. S. G. and G. A. E.; draft/revision of the article: C. R. G., Á. S. G., M. C. P. C., A. M. and C. M. V. All authors have read and agreed to the published version of the manuscript.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::07a9a105f2324e8c06cbab7cff9b2437Test
https://doi.org/10.1017/s0007114522000034Test

المؤلفون: Jiamei Shen, Xiaokun Geng, James Stevenson, Longfei Guan, Yuchuan Ding

المصدر: Environmental Disease, Vol 1, Iss 3, Pp 90-94 (2016)

مصطلحات موضوعية: Basilar artery, CD68, high cholesterol diet, internal carotid artery, intracranial atherosclerotic stenosis, middle cerebral artery, NG-nitro-L-arginine methyl ester, stroke, Public aspects of medicine, RA1-1270

الوصف: Atherosclerosis in the cerebral vasculature is a major risk factor for death and disability. However, atherosclerosis has been primarily studied in the coronary vasculature despite the distinct characteristics of the brain′s vessel morphometry and biochemistry. Recent preclinical work has established a rat model for studying intracranial atherosclerosis to optimize pharmacologic and lifestyle changes to prevent or reverse this disease. One such preventative strategy utilizes dietary omega-3 fatty acids (O3FAs), which have gained public interest due to its potential application in various diseases. A large number of studies of O3FA have examined the basic scientific framework, and this article examines its role as a dietary supplement to prevent the development of intracranial atherosclerotic stenosis.

وصف الملف: electronic resource

العلاقة: http://www.environmentmed.org/article.asp?issn=2468-5690;year=2016;volume=1;issue=3;spage=90;epage=94;aulast=ShenTest; https://doaj.org/toc/2468-5690Test; https://doaj.org/toc/2468-5704Test

الوصول الحر: https://doaj.org/article/2520a973780046bdaf45f79769f43fc8Test

المؤلفون: Dong-li Yang, Kun Zhou, Lin Li, Yan-yan Li, Yan-yan Wang, Yu-hong Li

المصدر: Biological and Pharmaceutical Bulletin. 2018, 41(9):1406

المؤلفون: Gomez, S.I, Strick, D.M., Romero, J.C.

المصدر: Brazilian Journal of Medical and Biological Research. February 2008 41(2)

مصطلحات موضوعية: Kidney, NG-nitro-L-arginine methyl ester, Meclofenamate, Renal circulation

الوصف: This study was undertaken in anesthetized dogs to evaluate the relative participation of prostaglandins (PGs) and nitric oxide (NO) in the maintenance of total renal blood flow (TRBF), and renal medullary blood flow (RMBF). It was hypothesized that the inhibition of NO should impair cortical and medullary circulation because of the synthesis of this compound in the endothelial cells of these two territories. In contrast, under normal conditions of perfusion pressure PG synthesis is confined to the renal medulla. Hence PG inhibition should predominantly impair the medullary circulation. The initial administration of 25 µM kg-1 min-1 NG-nitro-L-arginine methyl ester produced a significant 26% decrease in TRBF and a concomitant 34% fall in RMBF, while the subsequent inhibition of PGs with 5 mg/kg meclofenamate further reduced TRBF by 33% and RMBF by 89%. In contrast, the initial administration of meclofenamate failed to change TRBF, while decreasing RMBF by 49%. The subsequent blockade of NO decreased TRBF by 35% without further altering RMBF. These results indicate that initial PG synthesis inhibition predominantly alters the medullary circulation, whereas NO inhibition decreases both cortical and medullary flow. This latter change induced by NO renders cortical and RMBF susceptible to a further decrease by PG inhibition. However, the decrease in medullary circulation produced by NO inhibition is not further enhanced by subsequent PG inhibition.

وصف الملف: text/html

الوصول الحر: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200014Test

المؤلفون: S.I Gomez, D.M. Strick, J.C. Romero

المصدر: Brazilian Journal of Medical and Biological Research, Vol 41, Iss 2, Pp 170-175 (2008)

مصطلحات موضوعية: Kidney, NG-nitro-L-arginine methyl ester, Meclofenamate, Renal circulation, Medicine (General), R5-920, Biology (General), QH301-705.5

الوصف: This study was undertaken in anesthetized dogs to evaluate the relative participation of prostaglandins (PGs) and nitric oxide (NO) in the maintenance of total renal blood flow (TRBF), and renal medullary blood flow (RMBF). It was hypothesized that the inhibition of NO should impair cortical and medullary circulation because of the synthesis of this compound in the endothelial cells of these two territories. In contrast, under normal conditions of perfusion pressure PG synthesis is confined to the renal medulla. Hence PG inhibition should predominantly impair the medullary circulation. The initial administration of 25 µM kg-1 min-1 NG-nitro-L-arginine methyl ester produced a significant 26% decrease in TRBF and a concomitant 34% fall in RMBF, while the subsequent inhibition of PGs with 5 mg/kg meclofenamate further reduced TRBF by 33% and RMBF by 89%. In contrast, the initial administration of meclofenamate failed to change TRBF, while decreasing RMBF by 49%. The subsequent blockade of NO decreased TRBF by 35% without further altering RMBF. These results indicate that initial PG synthesis inhibition predominantly alters the medullary circulation, whereas NO inhibition decreases both cortical and medullary flow. This latter change induced by NO renders cortical and RMBF susceptible to a further decrease by PG inhibition. However, the decrease in medullary circulation produced by NO inhibition is not further enhanced by subsequent PG inhibition.

وصف الملف: electronic resource

العلاقة: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200014Test; https://doaj.org/toc/0100-879XTest; https://doaj.org/toc/1414-431XTest

الوصول الحر: https://doaj.org/article/097220c31c254a79bcfeff317e194b88Test

المؤلفون: Michelle L, O'Donoghue, Nicholas A, Marston

المصدر: Journal of the American College of Cardiology

مصطلحات موضوعية: L-NAME, NG-nitro-l-arginine methyl ester, C16:0-ceramide, sphingolipids, ScAT, subcutaneous adipose tissue, vascular redox state, eNOS, endothelial nitric oxide synthase, GLP-1, glucagon-like peptide-1, ThAT, thoracic adipose tissue, HAEC, human aortic endothelial cell, metabolomics, adipose tissue, NADPH, reduced nicotinamide adenine dinucleotide phosphate, Cer16:0, C16:0-ceramide, Adipose Tissue, GlcCer16:0, C16:0-glycosylceramide, Cardiovascular Diseases, cardiovascular disease, AT, adipose tissue, Humans, Obesity, PP2A, protein phosphatase 2, SPL, sphingolipid, Language, Original Investigation

الوصف: Background Obesity is associated with increased cardiovascular risk; however, the potential role of dysregulations in the adipose tissue (AT) metabolome is unknown. Objectives The aim of this study was to explore the role of dysregulation in the AT metabolome on vascular redox signaling and cardiovascular outcomes. Methods A screen was conducted for metabolites differentially secreted by thoracic AT (ThAT) and subcutaneous AT in obese patients with atherosclerosis (n = 48), and these metabolites were then linked with dysregulated vascular redox signaling in 633 patients undergoing coronary bypass surgery. The underlying mechanisms were explored in human aortic endothelial cells, and their clinical value was tested against hard clinical endpoints. Results Because ThAT volume was associated significantly with arterial oxidative stress, there were significant differences in sphingolipid secretion between ThAT and subcutaneous AT, with C16:0-ceramide and derivatives being the most abundant species released within adipocyte-derived extracellular vesicles. High ThAT sphingolipid secretion was significantly associated with reduced endothelial nitric oxide bioavailability and increased superoxide generated in human vessels. Circulating C16:0-ceramide correlated positively with ThAT ceramides, dysregulated vascular redox signaling, and increased systemic inflammation in 633 patients with atherosclerosis. Exogenous C16:0-ceramide directly increased superoxide via tetrahydrobiopterin-mediated endothelial nitric oxide synthase uncoupling and dysregulated protein phosphatase 2 in human aortic endothelial cells. High plasma C16:0-ceramide and its glycosylated derivative were independently related with increased risk for cardiac mortality (adjusted hazard ratios: 1.394; 95% confidence interval: 1.030 to 1.886; p = 0.031 for C16:0-ceramide and 1.595; 95% confidence interval: 1.042 to 2.442; p = 0.032 for C16:0-glycosylceramide per 1 SD). In a randomized controlled clinical trial, 1-year treatment of obese patients with the glucagon-like peptide-1 analog liraglutide suppressed plasma C16:0-ceramide and C16:0-glycosylceramide changes compared with control subjects. Conclusions These results demonstrate for the first time in humans that AT-derived ceramides are modifiable regulators of vascular redox state in obesity, with a direct impact on cardiac mortality in advanced atherosclerosis. (The Interaction Between Appetite Hormones; NCT02094183)
Central Illustration

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::14ef579448d417129a62eb72b5480cccTest
https://pubmed.ncbi.nlm.nih.gov/34016263Test