المؤلفون: Genah S., Cialdai F., Ciccone V., Sereni E., Morbidelli L., Monici M.

المساهمون: Genah, S., Cialdai, F., Ciccone, V., Sereni, E., Morbidelli, L., Monici, M.

مصطلحات موضوعية: wound healing, NIR laser radiation, inflammation, fibroblast, photobiomodulation

الوصف: The fine control of inflammation following injury avoids fibrotic scars or impaired wounds. Due to side effects by anti‐inflammatory drugs, the research is continuously active to define alternative therapies. Among them, physical countermeasures such as photobiomodulation therapy (PBMT) are considered effective and safe. To study the cellular and molecular events associated with the anti‐inflammatory activity of PBMT by a dual‐wavelength NIR laser source, human dermal fibroblasts were exposed to a mix of inflammatory cytokines (IL‐1β and TNF‐α) followed by laser treatment once a day for three days. Inducible inflammatory key enzymatic pathways, as iNOS and COX‐2/mPGES‐1/PGE2, were upregulated by the cytokine mix while PBMT reverted their levels and activities. The same behavior was observed with the proangiogenic factor vascular endothelial growth factor (VEGF), involved in neovascularization of granulation tissue. From a molecular point of view, PBMT retained NF‐kB cytoplasmatic localization. According to a change in cell morphology, differences in expression and distribution of fundamental cytoskeletal proteins were observed following treatments. Tubulin, F‐actin, and α‐SMA changed their organization upon cytokine stimulation, while PBMT reestablished the basal localization. Cytoskeletal rearrangements occurring after inflammatory stimuli were correlated with reorganization of membrane α5β1 and fibronectin network as well as with their upregulation, while PBMT induced significant downregulation. Similar changes were observed for collagen I and the gelatinolytic enzyme MMP‐1. In conclusion, the present study demonstrates that the proposed NIR laser therapy is effective in controlling fibroblast activation induced by IL‐1β and TNF‐α, likely responsible for a deleterious effect of persistent inflammation.

وصف الملف: ELETTRONICO

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/33809724; info:eu-repo/semantics/altIdentifier/wos/WOS:000633425200001; volume:9; issue:3; numberofpages:23; journal:BIOMEDICINES; http://hdl.handle.net/11365/1139308Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85103274246; https://www.mdpi.com/2227-9059/9/3/307Test

الإتاحة: https://doi.org/10.3390/biomedicines9030307Test
http://hdl.handle.net/11365/1139308Test
https://www.mdpi.com/2227-9059/9/3/307Test

المؤلفون: Shirley Genah, Francesca Cialdai, Valerio Ciccone, Elettra Sereni, Lucia Morbidelli, Monica Monici

المصدر: Biomedicines; Volume 9; Issue 3; Pages: 307

مصطلحات موضوعية: wound healing, NIR laser radiation, inflammation, fibroblasts, photobiomodulation

الوصف: The fine control of inflammation following injury avoids fibrotic scars or impaired wounds. Due to side effects by anti-inflammatory drugs, the research is continuously active to define alternative therapies. Among them, physical countermeasures such as photobiomodulation therapy (PBMT) are considered effective and safe. To study the cellular and molecular events associated with the anti-inflammatory activity of PBMT by a dual-wavelength NIR laser source, human dermal fibroblasts were exposed to a mix of inflammatory cytokines (IL-1β and TNF-α) followed by laser treatment once a day for three days. Inducible inflammatory key enzymatic pathways, as iNOS and COX-2/mPGES-1/PGE2, were upregulated by the cytokine mix while PBMT reverted their levels and activities. The same behavior was observed with the proangiogenic factor vascular endothelial growth factor (VEGF), involved in neovascularization of granulation tissue. From a molecular point of view, PBMT retained NF-kB cytoplasmatic localization. According to a change in cell morphology, differences in expression and distribution of fundamental cytoskeletal proteins were observed following treatments. Tubulin, F-actin, and α-SMA changed their organization upon cytokine stimulation, while PBMT reestablished the basal localization. Cytoskeletal rearrangements occurring after inflammatory stimuli were correlated with reorganization of membrane α5β1 and fibronectin network as well as with their upregulation, while PBMT induced significant downregulation. Similar changes were observed for collagen I and the gelatinolytic enzyme MMP-1. In conclusion, the present study demonstrates that the proposed NIR laser therapy is effective in controlling fibroblast activation induced by IL-1β and TNF-α, likely responsible for a deleterious effect of persistent inflammation.

وصف الملف: application/pdf

العلاقة: Gene and Cell Therapy; https://dx.doi.org/10.3390/biomedicines9030307Test

الإتاحة: https://doi.org/10.3390/biomedicines9030307Test

المؤلفون: Francesca Cialdai, Desire' Pantalone, Daniele Bani, Paolo Romagnoli, ANGELA M. RIZZO, Fabio Celotti, Alessandra Colciago, Elettra Sereni, Francesco Ranaldi and Monica Monici

المساهمون: F. Cialdai, D. Pantalone, D. Bani, P. Romagnoli, A.M. Rizzo, F.M. Celotti, A.M. Colciago, E. Sereni, F. Ranaldi and Monica Monici

مصطلحات موضوعية: Hirudo medicinalis (Annelida), Microgravity (μg), Platelet rich plasma (PRP), NIR laser radiation, in vivo model, Settore BIO/10 - Biochimica

العلاقة: ISGP Meeting & ESA Life Sciences Meeting : June, 18 - 22; numberofpages:2; journal:FRONTIERS IN PHYSIOLOGY; http://hdl.handle.net/2434/609170Test

الإتاحة: https://doi.org/10.3389/conf.fphys.2018.26.00037Test
http://hdl.handle.net/2434/609170Test

المؤلفون: Valerio Ciccone, Monica Monici, Elettra Sereni, Shirley Genah, Francesca Cialdai, Lucia Morbidelli

المصدر: Biomedicines, Vol 9, Iss 307, p 307 (2021)
Biomedicines
Volume 9
Issue 3

مصطلحات موضوعية: 0301 basic medicine, medicine.medical_treatment, Medicine (miscellaneous), Inflammation, wound healing, Article, General Biochemistry, Genetics and Molecular Biology, Proinflammatory cytokine, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Downregulation and upregulation, NIR laser radiation, fibroblasts, photobiomodulation, medicine, Fibroblast, lcsh:QH301-705.5, inflammation, Granulation tissue, Vascular endothelial growth factor, 030104 developmental biology, medicine.anatomical_structure, Cytokine, chemistry, lcsh:Biology (General), 030220 oncology & carcinogenesis, Cancer research, medicine.symptom, Wound healing

الوصف: The fine control of inflammation following injury avoids fibrotic scars or impaired wounds. Due to side effects by anti-inflammatory drugs, the research is continuously active to define alternative therapies. Among them, physical countermeasures such as photobiomodulation therapy (PBMT) are considered effective and safe. To study the cellular and molecular events associated with the anti-inflammatory activity of PBMT by a dual-wavelength NIR laser source, human dermal fibroblasts were exposed to a mix of inflammatory cytokines (IL-1β and TNF-α) followed by laser treatment once a day for three days. Inducible inflammatory key enzymatic pathways, as iNOS and COX-2/mPGES-1/PGE2, were upregulated by the cytokine mix while PBMT reverted their levels and activities. The same behavior was observed with the proangiogenic factor vascular endothelial growth factor (VEGF), involved in neovascularization of granulation tissue. From a molecular point of view, PBMT retained NF-kB cytoplasmatic localization. According to a change in cell morphology, differences in expression and distribution of fundamental cytoskeletal proteins were observed following treatments. Tubulin, F-actin, and α-SMA changed their organization upon cytokine stimulation, while PBMT reestablished the basal localization. Cytoskeletal rearrangements occurring after inflammatory stimuli were correlated with reorganization of membrane α5β1 and fibronectin network as well as with their upregulation, while PBMT induced significant downregulation. Similar changes were observed for collagen I and the gelatinolytic enzyme MMP-1. In conclusion, the present study demonstrates that the proposed NIR laser therapy is effective in controlling fibroblast activation induced by IL-1β and TNF-α, likely responsible for a deleterious effect of persistent inflammation.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c22ce4cc4e27c619fabebb4e63b5fe72Test
https://www.mdpi.com/2227-9059/9/3/307Test

المؤلفون: Vincelette, Rebecca Lee

مصطلحات موضوعية: Thermal lensing, Continuous-wave near-infrared laser radiation, Eye, Laser radiation, Retinal lesion, CW NIR laser radiation, Retina, Mathematical model

الوصف: This research was a collaborative effort between the Air Force Research Laboratory (AFRL) and the University of Texas to examine the laser-tissue interaction of thermal lensing induced by continuous-wave, CW, near-infrared, NIR, laser radiation in the eye and its influence on the formation of a retinal lesion from said radiation. CW NIR laser radiation can lead to a thermal lesion induced on the retina given sufficient power and exposure duration as related to three basic parameters; the percent of transmitted energy to, the optical absorption of, and the size of the laser-beam created at the retina. Thermal lensing is a well-known phenomenon arising from the optical absorption, and subsequent temperature rise, along the path of the propagating beam through a medium. Thermal lensing causes the laser-beam profile delivered to the retina to be time dependent. Analysis of a dual-beam, multidimensional, high-frame rate, confocal imaging system in an artificial eye determined the rate of thermal lensing in aqueous media exposed to 1110, 1130, 1150 and 1318-nm wavelengths was related to the power density created along the optical axis and linear absorption coefficient of the medium. An adaptive optics imaging system was used to record the aberrations induced by the thermal lens at the retina in an artificial eye during steady-state. Though the laser-beam profiles changed over the exposure time, the CW NIR retinal damage thresholds between 1110-1319-nm were determined to follow conventional fitting algorithms which neglected thermal lensing. A first-order mathematical model of thermal lensing was developed by conjoining an ABCD beam propagation method, Beer's law of attenuation, and a solution to the heat-equation with respect to radial diffusion. The model predicted that thermal lensing would be strongest for small (< 4-mm) 1/e² laser-beam diameters input at the corneal plane and weakly transmitted wavelengths where less than 5% of the energy is delivered to the retina. The model predicted thermal lensing would cause the retinal damage threshold for wavelengths above 1300-nm to increase with decreasing beam-diameters delivered to the corneal plane, a behavior which was opposite of equivalent conditions simulated without thermal lensing.
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وصف الملف: electronic

الإتاحة: http://hdl.handle.net/2152/15336Test