المؤلفون: Quillere, Isabelle, Dargel-Graffin, Céline, Lea, Peter J., Hirel, Bertrand

المساهمون: Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris Saclay (COmUE), Lancaster Environment Centre, Lancaster University

المصدر: ISSN: 2073-4395 ; Agronomy ; https://hal.inrae.fr/hal-02622513Test ; Agronomy, 2018, 8, pp.1-12. ⟨10.3390/agronomy8120309⟩ ; https://www.mdpi.com/journal/agronomyTest.

مصطلحات موضوعية: genetic diversity, maize, remobilization, silking, uptake, N-15-labeling, nitrogen, [SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering

الوصف: International audience ; The impact of nitrogen (N)-limiting conditions after silking on kernel yield (KY)-related traits and whole plant N management was investigated using fifteen maize lines representative of plant genetic diversity in Europe and America. A large level of genetic variability of these traits was observed in the different lines when post-silking fertilization of N was strongly reduced. Under such N-fertilization conditions, four different groups of lines were identified on the basis of KY and kernel N content. Although the pattern of N management, including N uptake and N use was variable in the four groups of lines, a number of them were able to maintain both a high yield and a high kernel N content by increasing shoot N remobilization. No obvious relationship between the genetic background of the lines and their mode of N management was found. When N was limiting after silking, N remobilization appeared to be a good predictive marker for identifying maize lines that were able to maintain a high yield and a high kernel N content irrespective of their female flowering date. The use of N remobilization as a trait to select maize genotypes adapted to low N input is discussed.

العلاقة: hal-02622513; https://hal.inrae.fr/hal-02622513Test; https://hal.inrae.fr/hal-02622513/documentTest; https://hal.inrae.fr/hal-02622513/file/2018_Quiller%C3%A9_Agronomy_1.pdfTest; PRODINRA: 465671; WOS: 000454722400037

الإتاحة: https://doi.org/10.3390/agronomy8120309Test
https://hal.inrae.fr/hal-02622513Test
https://hal.inrae.fr/hal-02622513/documentTest
https://hal.inrae.fr/hal-02622513/file/2018_Quiller%C3%A9_Agronomy_1.pdfTest

المؤلفون: Céline Dargel-Graffin, Isabelle Quilleré, Peter J. Lea, Bertrand Hirel

المساهمون: Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Lancaster University, Université Paris Saclay (COmUE), Lancaster Environment Centre, Hirel, Bertrand

المصدر: Agronomy, Vol 8, Iss 12, p 309 (2018)
Agronomy
Agronomy, MDPI, 2018
Volume 8
Issue 12
Agronomy, MDPI, 2018, 8, pp.1-12. ⟨10.3390/agronomy8120309⟩
Agronomy (8), 1-12. (2018)

مصطلحات موضوعية: [SDV]Life Sciences [q-bio], chemistry.chemical_element, Biology, silking, maize, nitrogen, lcsh:Agriculture, N-15-labeling, 03 medical and health sciences, Human fertilization, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Genetic variation, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Genetic variability, genetic diversity, remobilization, uptake, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 2. Zero hunger, 0303 health sciences, Genetic diversity, Crop yield, fungi, lcsh:S, food and beverages, 04 agricultural and veterinary sciences, Nitrogen, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, chemistry, Agronomy, Shoot, 15N-labeling, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, Agronomy and Crop Science, Plant nutrition

الوصف: International audience; The impact of nitrogen (N)-limiting conditions after silking on kernel yield (KY)-related traits and whole plant N management was investigated using fifteen maize lines representative of plant genetic diversity in Europe and America. A large level of genetic variability of these traits was observed in the different lines when post-silking fertilization of N was strongly reduced. Under such N-fertilization conditions, four different groups of lines were identified on the basis of KY and kernel N content. Although the pattern of N management, including N uptake and N use was variable in the four groups of lines, a number of them were able to maintain both a high yield and a high kernel N content by increasing shoot N remobilization. No obvious relationship between the genetic background of the lines and their mode of N management was found. When N was limiting after silking, N remobilization appeared to be a good predictive marker for identifying maize lines that were able to maintain a high yield and a high kernel N content irrespective of their female flowering date. The use of N remobilization as a trait to select maize genotypes adapted to low N input is discussed.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fae708244d79392a74f07b251fa5bb26Test
https://www.mdpi.com/2073-4395/8/12/309Test

المؤلفون: Arkoun, Mustapha, Jannin, Laetitia, Laine, Philippe, Etienne, Philippe, Masclaux-Daubresse, Celine, Citerne, Sylvie, Garnica, Maria, Garcia-Mina, Jose-Maria, Yvin, Jean-Claude, Ourry, Alain

المساهمون: Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Timac Agro Spain, Timac - Agro International, Pole de competitivite Mer-Bretagne, Fond Unique Inter-ministeriel

المصدر: ISSN: 0032-079X.

مصطلحات موضوعية: VOLATILIZATION, PHYTOTOXICITY, Urea, BnDUR3, N-15-labeling, Nickel, Phenylphosphorodiamidate (PPD), Enzymatic activities, Gene expressions, GLUTAMATE-DEHYDROGENASE, NITROGEN-METABOLISM, MICROBIAL UREASES, HIGHER-PLANTS, IN-VIVO, ARABIDOPSIS, TOBACCO, AMMONIA, [SDV]Life Sciences [q-bio]

الوصف: Urea is the major nitrogen (N) form supplied as fertilizer in agriculture. However, urease, a nickel-dependent enzyme, allows plants to use external or internally generated urea as a nitrogen source. Since a urease inhibitor is frequently applied in conjunction with urea fertilizer, the N-metabolism of plants may be affected. The aim of this study was to determine physiological and molecular effects of nickel deficiency and a urease inhibitor on urea uptake and assimilation in oilseed rape. Plants were grown on hydroponic solution with urea as the sole N source under three treatments: plants treated with nickel (+Ni) as a control, without nickel (-Ni) and with nickel and phenylphosphorodiamidate (+Ni+PPD). Urea transport and assimilation were investigated. The results show that Ni-deficiency or PPD supply led to reduced growth and reduced N-15-uptake from urea. This effect was more pronounced in PPD-treated plants, which accumulated high amounts of urea and ammonium. Thus, Ni-deficiency or addition of PPD, limit the availability of N and decreased shoot and root amino acid content. The up-regulation of BnDUR3 in roots indicated that this gene is a component of the stress response to nitrogen-deficiency. A general decline of glutamine synthetase (GS) activity and activation of glutamate dehydrogenase (GDH) and increases in its expression level were observed in control plants. At the same time, in (-N) or (+Ni+PPD) treated plants, no increases in GS or GDH activities and expression level were found. Overall results showed that plants require Ni as a nutrient (while most widely used nutrient solutions are devoid of Ni), whether they are grown with or without a urea supply, and that urease inhibitors may have deleterious effects at least in hydroponic grown oilseed rape.

العلاقة: hal-01190672; https://hal.science/hal-01190672Test; PRODINRA: 211683; WOS: 000312729400007

الإتاحة: https://doi.org/10.1007/s11104-012-1227-2Test
https://hal.science/hal-01190672Test

المؤلفون: Maria Garnica, Jean-Claude Yvin, Philippe Laîné, Mustapha Arkoun, Céline Masclaux-Daubresse, Laëtitia Jannin, Philippe Etienne, José-Maria Garcia-Mina, Sylvie Citerne, Alain Ourry

المساهمون: Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Timac Agro Spain, Timac - Agro nternational, Pole de competitivite Mer-Bretagne, Fond Unique Inter-ministeriel, Ecophysiologie Végétale, Agronomie et Nutritions ( EVA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Institut Jean-Pierre Bourgin ( IJPB ), Institut National de la Recherche Agronomique ( INRA ) -AgroParisTech, Timac Agro Int

المصدر: Plant and Soil
Plant and Soil, Springer Verlag, 2013, 362 (1-2), pp.79-92. ⟨10.1007/s11104-012-1227-2⟩
Plant and Soil, Springer Verlag, 2013, 362 (1-2), pp.79-92. 〈10.1007/s11104-012-1227-2〉

مصطلحات موضوعية: 0106 biological sciences, NITROGEN-METABOLISM, BnDUR3, Urease, Enzymatic activities, [SDV]Life Sciences [q-bio], Soil Science, Plant Science, engineering.material, 01 natural sciences, HIGHER-PLANTS, 03 medical and health sciences, chemistry.chemical_compound, Ammonia, N-15-labeling, Nickel, PHYTOTOXICITY, Urea, Ammonium, VOLATILIZATION, Food science, MICROBIAL UREASES, IN-VIVO, 030304 developmental biology, 2. Zero hunger, Phenylphosphorodiamidate (PPD), 0303 health sciences, TOBACCO, AMMONIA, biology, [ SDV ] Life Sciences [q-bio], GLUTAMATE-DEHYDROGENASE, Glutamate dehydrogenase, ARABIDOPSIS, Urea transport, chemistry, Agronomy, engineering, biology.protein, Phytotoxicity, Fertilizer, Gene expressions, 010606 plant biology & botany

الوصف: Urea is the major nitrogen (N) form supplied as fertilizer in agriculture. However, urease, a nickel-dependent enzyme, allows plants to use external or internally generated urea as a nitrogen source. Since a urease inhibitor is frequently applied in conjunction with urea fertilizer, the N-metabolism of plants may be affected. The aim of this study was to determine physiological and molecular effects of nickel deficiency and a urease inhibitor on urea uptake and assimilation in oilseed rape. Plants were grown on hydroponic solution with urea as the sole N source under three treatments: plants treated with nickel (+Ni) as a control, without nickel (-Ni) and with nickel and phenylphosphorodiamidate (+Ni+PPD). Urea transport and assimilation were investigated. The results show that Ni-deficiency or PPD supply led to reduced growth and reduced N-15-uptake from urea. This effect was more pronounced in PPD-treated plants, which accumulated high amounts of urea and ammonium. Thus, Ni-deficiency or addition of PPD, limit the availability of N and decreased shoot and root amino acid content. The up-regulation of BnDUR3 in roots indicated that this gene is a component of the stress response to nitrogen-deficiency. A general decline of glutamine synthetase (GS) activity and activation of glutamate dehydrogenase (GDH) and increases in its expression level were observed in control plants. At the same time, in (-N) or (+Ni+PPD) treated plants, no increases in GS or GDH activities and expression level were found. Overall results showed that plants require Ni as a nutrient (while most widely used nutrient solutions are devoid of Ni), whether they are grown with or without a urea supply, and that urease inhibitors may have deleterious effects at least in hydroponic grown oilseed rape.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0cc1284adb511627a408e631081c6c9Test
https://hal.archives-ouvertes.fr/hal-01190672Test

المؤلفون: Niculae, Anca, Bayer, Peter, Cirstea, Ion, Bergbrede, Tim, Pietrucha, Regina, Gruen, Mathias, Breitling, Reinhard, Alexandrov, Kirill

مصطلحات موضوعية: N-15-labeling, Recombinant protein, Eukaryotic expression system, Green fluorescent protein (GFP), Insect cells, Amino-acids, Purification, System, 0304 Medicinal and Biomolecular Chemistry, 06 Biological Sciences, 0601 Biochemistry and Cell Biology

الوصف: Isotope labeling of recombinant proteins is a prerequisite for application of nuclear magnetic resonance spectroscopy (NMR) for the characterization of the three-dimensional structures and dynamics of proteins. Overexpression of isotopically labeled proteins in bacterial or yeast host organisms has several drawbacks. In this work, we tested whether the recently described eukaryotic protein expression system based on the protozoa Leishmania tarentolae could be used for production of amino acid specific 15N-labeled recombinant proteins. Using synthetic growth medium we were able to express in L. tarentolae and purify to homogeneity (15)N-valine labeled Enchanced Green Fluorescent Protein (EGFP) with the final yield of 5.7 mg/liter of suspension culture. NMR study of isolated EGFP illustrated the success of the labeling procedure allowing identification of all 18 valine residues of the protein in the HSQC spectrum. Our results demonstrate the suitability of the L. tarentolae expression system for production of isotopically labeled proteins.

العلاقة: orcid:0000-0002-0957-6511

الإتاحة: https://doi.org/10.1016/j.pep.2006.04.006Test
https://espace.library.uq.edu.au/view/UQ:186517Test