المؤلفون: Bacanelli, Gisele, Olarte, Larissa C., Silva, Marcio Roberto, Rodrigues, Rudielle A., Carneiro, Paulo A. M., Kannene, John B., Pasquatti, Taynara N., Takatani, Haruo, Zumarraga, Martin Jose, Etges, Rodrigo N., Araujo, Flabio Ribeiro de, Verbisck, Newton V.

المصدر: The Journal of Veterinary Medical Science 81 (10) : 1400-1408 (Octubre 2019)

مصطلحات موضوعية: Tuberculosis, Infeccion por Mycobacterium bovis, Tratamiento Ionizante, Desorción, Espectrometría, Infeccion Mycobacterium tuberculosis, Bovina, Mycobacterium bovis Infections, Ionization Treatment, Desorption, Spectrometry, Mycobacterium tuberculosis Infections, Bovinae

الوصف: In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future. ; Instituto de Biotecnología ; Fil: Bacanelli, Gisele. Federal University of Mato Grosso do Sul. Biotechnology and Biodiversity of the Central Western Region Postgraduate Program; Brasil ; Fil: Olarte, Larissa C. Federal University of Mato Grosso do Sul. Biochemistry and Molecular Biology Multicentric Postgraduate Program; Brasil ; Fil: Silva, Marcio Roberto. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Leite; Brasil ; Fil: Rodrigues, Rudielle A. Federal University of Mato Grosso do Sul. Faculty of Veterinary Medicine. Veterinary Sciences Postgraduate Program; Brasil ; Fil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados Unidos ; Fil: Kannene, John B. Michigan State University. Center for Comparative Epidemiology; Estados Unidos ; Fil: Pasquatti, Taynara N. Dom Bosco Catholic University; Brasil ; Fil: Takatani, Haruo. Agricultural Defense Agency of Amazonas; Brasil ; Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: ...

وصف الملف: application/pdf

العلاقة: https://www.jstage.jst.go.jp/article/jvms/81/10/81_19-0214/_articleTest; http://hdl.handle.net/20.500.12123/6567Test; https://doi.org/10.1292/jvms.19-0214Test

الإتاحة: https://doi.org/20.500.12123/6567Test
https://doi.org/10.1292/jvms.19-0214Test
https://hdl.handle.net/20.500.12123/6567Test
https://www.jstage.jst.go.jp/article/jvms/81/10/81_19-0214/_articleTest

المؤلفون: Gamietea, Ignacio José, Garbaccio, Sergio Gabriel

مصطلحات موضوعية: Reproducción Animal, Técnicas de Diagnosis, Destete, Condición Corporal, Enfermedades de los Animales, Infeccion por Mycobacterium bovis, Tuberculosis, Forrajes, Vaca, Salud Reproductiva, Animal Reproduction, Diagnosis Techniques, Weaning, Body Conditions, Animal Diseases, Mycobacterium bovis Infections, Forages, Cows, Reproductive Health

الوصف: Trata de cuatro acciones clave en los rodeos de cría bovina de la provincia de Buenos Aires. Las cuatro están ligadas al periodo postservicio: evaluaciones ginecológica, de estado corporal y dentaria de los vientres y destete de los terneros. Se describen ítems a tener en cuenta para avanzar en el control de la Tuberculosis bovina. Se analiza la tasa de crecimiento forrajero del pastizal de la zona. ; EEA San Pedro ; Fil: Gamietea, Ignacio José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro. Agencia de Extensión Agropecuaria San Pedro; Argentina ; Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina

وصف الملف: application/pdf

العلاقة: https://inta.gob.ar/sites/default/files/intasp_panorama_ganadero_nro7.pdfTest; http://hdl.handle.net/20.500.12123/7792Test

الإتاحة: https://doi.org/20.500.12123/7792Test
https://hdl.handle.net/20.500.12123/7792Test
https://inta.gob.ar/sites/default/files/intasp_panorama_ganadero_nro7.pdfTest

المؤلفون: Garcia, Elizabeth Andrea, Bianco, Marí­a Veronica, Blanco, Federico Carlos, Bigi, Fabiana

المصدر: Journal Of Infection In Developing Countries 10 (8): 892-894 (August 2016)

مصطلحات موضوعية: Enfermedades de los Animales, Mycobacterium Bovis, Tuberculosis, Infección por Mycobacterium Bovis, Animal Diseases, Mycobacterium Bovis Infections

الوصف: Here we confirm the efficacy of MbΔmce2-phoP to protect mice against bTB challenge and we demonstrated that this new attenuated strain is safer than our previous bTB vaccine MbΔmce2. Altogether these results support the further assessment of this candidate in cattle model of bTB.Experiments with mice were performed in compliance with the regulations of the Institutional Animal Care and Use Committee (CICUAE) of INTA (61/2014). ; Inst. de Biotecnología ; Fil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina ; Fil: Bianco, Marí­a Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina ; Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina

وصف الملف: application/pdf

العلاقة: http://hdl.handle.net/20.500.12123/1053Test; https://jidc.org/index.php/journal/article/view/27580338/1568Test; García E, Bianco M, Blanco F, Bigi F (2016) Mycobacterium bovis deleted in mce2 and phoP loci protects C57BL/6 mice against tuberculosis. The Journal Of Infection In Developing Countries 10 (08): 892-894. https://doi.org/10.3855/jidc.7721Test; 2036-6590 (Print); 1972-2680 (Online); https://doi.org/10.3855/jidc.7721Test

الإتاحة: https://doi.org/20.500.12123/1053Test
https://doi.org/10.3855/jidc.7721Test
https://hdl.handle.net/20.500.12123/1053Test
https://jidc.org/index.php/journal/article/view/27580338/1568Test

المؤلفون: Garbaccio, Sergio Gabriel, Barandiaran, Soledad, Fernández, Analía, Macias, Analía, Magnano, Gabriel, Martinez Vivot, Marcela, Peyru, Maite, Cataldi, Angel Adrian

المصدر: Revista argentina de microbiología 48 (2) : 161-165. (April–June 2016)

مصطلحات موضوعية: Enfermedades de los Animales, Mycobacterium Bovis, Aislamiento, Infección por Mycobacterium Bovis, Animal Diseases, Isolation, Mycobacterium Bovis Infections, Lesiones Granulomatosas

الوصف: Mycobacterium bovis es el agente etiológico de la tuberculosis bovina. La confirmación diagnóstica en el laboratorio se realiza a través del aislamiento bacteriológico. Los ensayos interlaboratorio permiten evaluar el desempe˜no de cada laboratorio participante comparándolo con otros de capacidades similares. El objetivo del presente estudio fue determinar la eficiencia en el aislamiento de M. bovis. Este estudio contó con la participación de 4 laboratorios, los cuales procesaron a ciego 25 muestras de tejidos con lesiones, seleccionadas previamente por aislamiento. El laboratorio A obtuvo la mayor proporción de aislamientos (68%), seguido del C (60%) y luego del B y D (ambos con el 52%). Las mayores concordancias se observaron entre los pares de laboratorios B-C y B-D (68%). Las diferencias pudieron deberse a factores propios de los procedimientos en cada laboratorio. Este tipo de análisis interlaboratorio permite evidenciar posibles errores en los procedimientos bacteriológicos e identificar puntos críticos del proceso para detectar M. bovis de manera eficiente. ; Mycobacterium bovis is the causative agent of bovine tuberculosis. The diagnostic laboratory confirmation is made through bacterial isolation. The aim of interlaboratory tests is to assess the performance of each participant in comparison with other of similar capacities. The test objective was to determine the efficiency of isolation of M. bovis. Four laboratories were part of the test and processed 25 blind tissue samples from granulomatous lesions and with previous M. bovis isolation. The laboratory that had the highest proportion of isolates was A (68%), followed by C (60%) and then B and D (both with 52%). The greatest concordance was observed between B-D and B-C laboratories (68%). The differences could be due to specific factors in each laboratory procedures. This type of interlaboratory tests highlights errors in the bacteriology and identifies critical points in the process to detect M. bovis accurately. ; Inst. de Patobiología ; Fil: ...

وصف الملف: application/pdf

العلاقة: http://hdl.handle.net/20.500.12123/1002Test; http://ac.els-cdn.com/S0325754116300025/1-s2.0-S0325754116300025-main.pdf?_tid=a475b2c0-874d-11e7-bf2e-00000aacb360&acdnat=1503415503_c249bbaa0f127df52f8f32d140d228b2Test; http://www.scielo.org.ar/pdf/ram/v48n2/v48n2a13.pdfTest; https://doi.org/10.1016/j.ram.2016.03.004Test

الإتاحة: https://doi.org/20.500.12123/1002Test
https://doi.org/10.1016/j.ram.2016.03.004Test
https://hdl.handle.net/20.500.12123/1002Test
http://ac.els-cdn.com/S0325754116300025/1-s2.0-S0325754116300025-main.pdf?_tid=a475b2c0-874d-11e7-bf2e-00000aacb360&acdnat=1503415503_c249bbaa0f127df52f8f32d140d228b2Test
http://www.scielo.org.ar/pdf/ram/v48n2/v48n2a13.pdfTest

المؤلفون: Griffa, Natanael, Moyano, Roberto Damián, Canal, Ana María, Traveria, Gabriel Eduardo, Santangelo, María De La Paz, Alonso, Maria Natalia, Romano, Maria Isabel

المصدر: The Veterinary Journal 256 : 105426 (Febrero 2020)

مصطلحات موضوعية: Tuberculosis, Infeccion por Mycobacterium bovis, ELISA, Mycobacterium bovis Infections, Tests, Humoral Response, Respuesta Humoral

الوصف: Programs for the eradication of bovine tuberculosis (bTB) focus on the tuberculin skin test (TST) and slaughter of reactor cattle. However, the disease remains an animal health concern in several countries and improving the efficiency of the TST has become a critical issue. The detection of Mycobacterium bovis antibodies in serum, within weeks after the TST, may be a rapid and inexpensive way to improve bTB control. This study reports the validation of an enzyme-linked immunosorbent assay (ELISA) to detect bovine tuberculosis as an ancillary test to TST in dairy farms in Argentina. The estimated validation parameters were within the established requirements of the World Organization for Animal Health (OIE). The test demonstrated high repeatability, with coefficients of variation <25%. High test reproducibility through interlaboratory testing was also found, with an estimated Pearson coefficient of 0.9648 (95% confidence intervals 0.9315–0.9820). The ELISA detected tuberculous cattle unidentified by the TST. Of 43 animals sent to slaughterhouses that were ELISA positive 15–17 days after a negative TST, 36 were confirmed as infected with M. bovis by histopathology and IS6110 PCR. According to ROC curve analysis of results of 145 cattle from M. bovis-free herds and the 36 M. bovis-infected cattle, at a corrected optical density cut-off point of 0.3853, specificity was 95.95% and the positive predictive value at this cut-off was 83.72%. The ELISA detection test validated in this study could be readily applied in dairy farms, to complement a prior TST and improve livestock health. ; Instituto de Biotecnologìa ; Fil: Griffa Natanael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Canal, Ana María. ...

وصف الملف: application/pdf

العلاقة: http://hdl.handle.net/20.500.12123/7787Test; https://www.sciencedirect.com/science/article/abs/pii/S1090023320300034Test; https://doi.org/10.1016/j.tvjl.2020.105426Test

الإتاحة: https://doi.org/20.500.12123/7787Test
https://doi.org/10.1016/j.tvjl.2020.105426Test
https://hdl.handle.net/20.500.12123/7787Test
https://www.sciencedirect.com/science/article/abs/pii/S1090023320300034Test

المؤلفون: Eirin, Maria Emilia, Carignano, Hugo Adrian, Shimizu, Ernesto, Pando, Maria de los Angeles, Zumarraga, Martin Jose, Magnano, Gabriel, Macías, Analía, Garbaccio, Sergio Gabriel, Huertas, Pablo Sebastian, Morsella, Claudia Graciela, Ferrara Muñiz, Ximena, Cataldi, Angel Adrian, Paolicchi, Fernando, Poli, Mario Andres

المصدر: Research in Veterinary Science 130 : 118-125 (June 2020)

مصطلحات موضوعية: Ganado Bovino, Enfermedades de los Animales, Polimorfismo, Tuberculosis, Mycobacterium bovis, Cattle, Animal Diseases, Polymorphism, Mycobacterium bovis Infections, Antigens, Infeccion por Mycobacterium bovis, Antígenos, Tuberculosis Bovina, Bovine Leucocyte Antigen

الوصف: Bovine tuberculosis (bTB) is caused by Mycobacterium bovis and disseminated worldwide. In Argentina, the highest prevalence occurs in dairy areas. BoLA DRB3.2 is related to the adaptive immunity in mycobacterial infections. Genetic polymorphisms of this marker have been associated with resistance or susceptibility to bovine diseases. We evaluated the association between BoLA DRB3.2 polymorphisms and bTB pathology scores in dairy and beef cattle breeds of Argentina. Most bovines exhibited visible lesions compatible with tuberculosis and, furthermore, 150 (85.7%) were also positive by bacteriology. A pathology index showed a variable degree of disease, from 3 to 76 (median pathology score = 9 (IQR: 7–15)). Thirty-five BoLA DRB3.2 alleles were identified with an associated frequency from 16% to 0.3%, distributed 73% (n = 128) in heterozygosis and 27% (n = 47) in homozygosis, with 12 BoLA DRB3.2 alleles (*0101, *1101, *1501, *0201, *2707 *1001, *1002, *1201, *14011, *0501 *0902 and *0701) representing the 74.7% of the population variability. A functional analysis grouped them in 4 out of 5 clusters (A-D), suggesting a functional overlapping. Among the 90 identified genotypes, *1101/*1101, *1101/*1501 and *0101/*0101 were the most frequent (10%, 8.9% and 8.9%, respectively). No association was detected between the pathology scores and a specific DRB3.2 allele (p > .05). Animals infected with M. bovis spoligotype SB0153 showed a significantly higher pathology score than those affected by the spoligotype SB0145 (p = .018). Furthermore, the Aberdeen Angus breed exhibited highest pathological scores (p < .0001), which were associated with disseminated lesion, thus suggesting that the host component could be important to the disease progression. ; Instituto de Biotecnología ; Fil: Eirin, Maria Emilia.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Carignano, Hugo Adrian. Instituto Nacional de ...

وصف الملف: application/pdf

العلاقة: http://hdl.handle.net/20.500.12123/7706Test; https://www.sciencedirect.com/science/article/abs/pii/S0034528819305624Test; https://doi.org/10.1016/j.rvsc.2020.03.001Test

الإتاحة: https://doi.org/20.500.12123/7706Test
https://doi.org/10.1016/j.rvsc.2020.03.001Test
https://hdl.handle.net/20.500.12123/7706Test
https://www.sciencedirect.com/science/article/abs/pii/S0034528819305624Test

المؤلفون: Vial, F, Johnston, WT, Donnelly, CA

المساهمون: Medical Research Council (MRC)

مصطلحات موضوعية: Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, MYCOBACTERIUM-BOVIS INFECTIONS, REPUBLIC-OF-IRELAND, HUSBANDRY PRACTICES, WILD BOAR, ENGLAND, ASSOCIATION, BREAKDOWNS, FARMS, EPIDEMIOLOGY, STRATEGIES, Animals, Cattle, Health Surveys, Immunity, Herd, Models, Biological, Multivariate Analysis, Mustelidae, Population Dynamics, Reproducibility of Results, Risk Factors, Time Factors, Tuberculosis, Bovine, United Kingdom, Great Britain

الوصف: Background: The control of bovine tuberculosis (bTB) remains a priority on the public health agenda in Great Britain, after launching in 1998 the Randomised Badger Culling Trial (RBCT) to evaluate the effectiveness of badger (Meles meles) culling as a control strategy. Our study complements previous analyses of the RBCT data (focusing on treatment effects) by presenting analyses of herd-level risks factors associated with the probability of a confirmed bTB breakdown in herds within each treatment: repeated widespread proactive culling, localized reactive culling and no culling (survey-only). Methodology/Principal Findings: New cases of bTB breakdowns were monitored inside the RBCT areas from the end of the first proactive badger cull to one year after the last proactive cull. The risk of a herd bTB breakdown was modeled using logistic regression and proportional hazard models adjusting for local farm-level risk factors. Inside survey-only and reactive areas, increased numbers of active badger setts and cattle herds within 1500 m of a farm were associated with an increased bTB risk. Inside proactive areas, the number of M. bovis positive badgers initially culled within 1500 m of a farm was the strongest predictor of the risk of a confirmed bTB breakdown. Conclusions/Significance: The use of herd-based models provide insights into how local cattle and badger populations affect the bTB breakdown risks of individual cattle herds in the absence of and in the presence of badger culling. These measures of local bTB risks could be integrated into a risk-based herd testing programme to improve the targeting of interventions aimed at reducing the risks of bTB transmission.

العلاقة: PLOS One; http://hdl.handle.net/10044/1/48844Test; https://dx.doi.org/10.1371/journal.pone.0018058Test; G0600719B

الإتاحة: https://doi.org/10.1371/journal.pone.0018058Test
http://hdl.handle.net/10044/1/48844Test

المؤلفون: Blanco, Federico Carlos, Bianco, María Veronica, Meikle, Virginia, Garbaccio, Sergio Gabriel, Vagnoni, Lucas Emilio, Cataldi, Angel Adrian, Bigi, Fabiana

المصدر: RIA 37 (2) : 189-192 (agosto 2011)

مصطلحات موضوعية: Ganado Bovino, Enfermedades de los Animales, Mycobacterium Bovis, Respuesta Inmunológica, Ternero, Infección Experimental, Cattle, Animal Diseases, Immune Response, Calves, Experimental Infection, Mycobacterium Bovis Infections, Infección por Mycobacterium Bovis

الوصف: La tuberculosis bovina es una enfermedad zoonótica producida por Mycobacterium bovis que afecta a animales de producción causando pérdidas económicas y representa, además, un problema para la salud pública. El conocimiento de la respuesta inmune inducida en el ganado bovino (el principal hospedador de M. bovis), durante la tuberculosis bovina es esencial para el desarrollo exitoso de medidas de control de la enfermedad. En este trabajo se determinó el perfil de respuesta celular que se desarrolla en terneros experimentalmente infectados con variadas dosis de M. bovis. Se observó que la respuesta celular CD4 es independiente de la dosis de inóculo, mientras que la respuesta celular CD8 es mínima cuando se infectan los animales a bajas dosis. También se observó que el nivel de producción de interferón gama (IFN-ɣ) por células monocíticas de sangre periférica no se asocia a la dosis de inóculo. ; Mycobacterium bovis, the causative agent of bovine tuberculosis, infects animals of agricultural importance and others mammals, including humans. Therefore, this disease causes significant economical losses and is a public health hazard. To better understand the immune response against M. bovis in cattle, in this study we characterized the immunological profile towards M. bovis antigens in cattle experimentally infected at various doses of a virulent M. bovis strain by measurement of IFN-ɣ production in peripheral blood mononuclear cells and by determining which lymphocyte subsets are involved in recall responses of peripheral blood mononuclear cells from infected cattle to M. bovis antigens. We found that the level of CD4 cell activation is similar among infected group irrespectively of the doses used to infect the animals, while activation of CD8 cells is dose-dependent. We also found that the production of IFN-ɣ in peripheral blood mononuclear cells after specific stimuli is not associated with the bacterial counts in the infectious doses. ; Instituto de Biotecnología ; Fil: Blanco, Federico Carlos. Instituto Nacional ...

وصف الملف: application/pdf

العلاقة: http://ria.inta.gob.ar/sites/default/files/numeros/ria37-2-2011_0.pdfTest; http://hdl.handle.net/20.500.12123/6410Test

الإتاحة: https://doi.org/20.500.12123/6410Test
https://hdl.handle.net/20.500.12123/6410Test
http://ria.inta.gob.ar/sites/default/files/numeros/ria37-2-2011_0.pdfTest

المؤلفون: Bigi, María Mercedes, Vazquez, Cristina Lourdes, Castelão, Ana Beatriz C., Garcia, Elizabeth Andrea, Cataldi, Angel Adrian, Jackson, Mary, McNeil, Michael, Soria, Marcelo Abel, Zumarraga, Martin Jose, Cabruja, Matias, Gago, Gabriela, Blanco, Federico Carlos, Nishibe, Christiane, Almeida Junior, Nalvo Franco de, Araujo, Flabio Ribeiro de, Bigi, Fabiana

المصدر: Veterinary microbiology 239 : 108482. (December 2019)

مصطلحات موضوعية: Mycobacterium Bovis, Genomas, Virulencia, Mutación, Patogenicidad, Bovina, Tuberculosis, Infección por Mycobacterium Bovis, Vacuna, Genomes, Virulence, Mutation, Pathogenicity, Bovinae, Mycobacterium Bovis Infections, Vaccines

الوصف: Mycobacterium bovis (M. bovis) is the causative agent of bovine tuberculosis, a chronic infectious disease that can affect cattle, other domesticated species, wild animals and humans. This disease produces important economic losses worldwide. Two M. bovis strains (04-303 and 534) have been isolated in Argentina. Whereas the 04-303 strain was isolated from a wild boar, the 534 strain was obtained from cattle. In a previous study, six weeks after infection, the 04-303 strain induced 100% mortality in mice. By contrast, mice infected with the 534 strain survived, with limited tissue damage, after four months. In this study we compared all predictive proteins encoded in both M. bovis genomes. The comparative analysis revealed 141 polymorphic proteins between both strains. From these proteins, nine virulence proteins showed polymorphisms in 04-303, whereas five did it in the 534 strain. Remarkably, both strains contained a high level of polymorphism in proteins related to phthiocerol dimycocerosate (PDIM) synthesis or transport. Further experimental evidence indicated that only mutations in the 534 strain have an impact on PDIM synthesis. The observed reduction in PDIM content in the 534 strain, together with its low capacity to induce phagosome arrest, may be associated with the reported deficiency of this strain to replicate and survive inside bovine macrophages. The findings of this study could contribute to a better understanding of pathogenicity and virulence aspects of M. bovis, which is essential for further studies aiming at developing new vaccines and diagnostic techniques for bovines. ; Instituto de Biotecnología ; Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: ...

وصف الملف: application/pdf

العلاقة: http://hdl.handle.net/20.500.12123/6681Test; https://www.sciencedirect.com/science/article/pii/S0378113519301099?via%3DihubTest; https://doi.org/10.1016/j.vetmic.2019.108482Test

الإتاحة: https://doi.org/20.500.12123/6681Test
https://doi.org/10.1016/j.vetmic.2019.108482Test
https://hdl.handle.net/20.500.12123/6681Test
https://www.sciencedirect.com/science/article/pii/S0378113519301099?via%3DihubTest

المؤلفون: Garbaccio, Sergio Gabriel, Garro, Carlos Javier, Delgado, Fernando Oscar, Tejada, G. A., Eirin, Maria Emilia, Huertas, Pablo Sebastian, Leon, Emilio Arnaldo, Zumarraga, Martin Jose

المصدر: Tuberculosis 117: 56–61. (Julio 2019)

مصطلحات موضوعية: Mycobacterium Bovis Infections, Skin Tests, Cattle, Infección por Mycobacterium Bovis, ELISA, Tuberculosis, Pruebas Cutáneas, Ganado Bovino

الوصف: Diagnostic tests based on cell-mediated immunity are used in programs for the control and eradication of bovine tuberculosis (bTB), which is mainly caused by Mycobacterium bovis. Additional serological assays could be performed as an ancillary method to detect an infected animal that fails to produce an immune response against the intradermal reaction (IDR), the official bTB test. In this study, we evaluated the effectiveness of an enzyme-linked immunosorbent assay (ELISA) that uses bovine PPD as a capture antigen as a complement to the IDR in herds with confirmed cases of bTB. The study was conducted in two stages. First, a panel of 200 serum samples was analyzed by ELISA. The sensitivity and specificity obtained were 60% and 99%, respectively. The subsequent stage consisted of evaluating 7,494 bovines from 14 selected dairy farms. The number of animals yielding a IDR negative/ELISA positive result were 200. A necropsy analysis of 33 of these IDR negative/ELISA positive animals revealed that 30 (91%) presented granulomatous lesions and positive M. bovis isolation. This finding confirmed bTB in most cases. Altogether, the results obtained in the present study suggest that the combined use of IDR and ELISA is an effective strategy to improve the control of bTB in endemic herds. ; Instituto de Patobiología ; Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina ; Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina ; Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina ; Fil: Tejada, G. A. Colón Diagnosis Laboratory Services; Argentina ; Fil: Eirin, Maria Emilia.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Huertas, Pablo Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). ...

وصف الملف: application/pdf

العلاقة: info:eu-repograntAgreement/INTA/PNSA/1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional.; https://www.sciencedirect.com/science/article/pii/S1472979218303512Test; http://hdl.handle.net/20.500.12123/6307Test; https://doi.org/10.1016/j.tube.2019.05.006Test

الإتاحة: https://doi.org/20.500.12123/6307Test
https://doi.org/10.1016/j.tube.2019.05.006Test
https://hdl.handle.net/20.500.12123/6307Test
https://www.sciencedirect.com/science/article/pii/S1472979218303512Test