المؤلفون: Jon Roger Eidet, Evan Michael Vallenari, Tor Paaske Utheim, Hans Christian Dalsbotten Aass, Ayyad Ahmad Zartasht Khan, Morten Carsten Moe, Dipak Sapkota

المصدر: Current Eye Research. 46:504-514

مصطلحات موضوعية: cis-trans-Isomerases, Cell Survival, Induced Pluripotent Stem Cells, Retinal Pigment Epithelium, Sericin, Cell Line, 03 medical and health sciences, Cellular and Molecular Neuroscience, Pigment, chemistry.chemical_compound, 0302 clinical medicine, medicine, Humans, Lactic Acid, Sericins, Induced pluripotent stem cell, Cells, Cultured, Retinal pigment epithelium, Monophenol Monooxygenase, Chemistry, food and beverages, Retinal, Hydrogen-Ion Concentration, Flow Cytometry, eye diseases, Sensory Systems, In vitro, Cell biology, Ophthalmology, Glucose, medicine.anatomical_structure, SILK, visual_art, Zonula Occludens-1 Protein, 030221 ophthalmology & optometry, visual_art.visual_art_medium, sense organs, 030217 neurology & neurosurgery, gp100 Melanoma Antigen

الوصف: Purpose Maintaining mature and viable retinal pigment epithelial cells (RPE) in vitro has proven challenging. Investigating compounds that can promote RPE-viability and maturation is motivated by RPE transplantation research, the quest to understand RPE physiology, and a desire to modulate RPE in pathological states. We have previously reported that the silk protein sericin promotes viability, maturation, and pigmentation of human fetal RPE. In the present study, our aim was to uncover whether these effects can be seen in adult retinal pigment epithelial cell line-19 (ARPE-19) and induced pluripotent stem cell-derived RPE (iPSC-RPE). Methods ARPE-19 and iPSC-RPE were cultured with or without 10 mg/mL sericin. After 7 days, viability was assessed with calcein-acetoxymethyl ester (CAM) and ethidium homodimer-1 (EH-1) assays, flow cytometry, and morphometric analysis. Expression levels of RPE65, tyrosinase, and Pmel17 were quantified to compare maturation between the sericin-treated and control cultures. Light microscopy and staining of the tight junction protein zonula occludens protein 1 (ZO-1) were employed to study sericin’s effects on RPE morphology. We also measured culture medium pH, glucose, lactate, and extracellular ion content. Results Sericin-supplemented RPE cultures demonstrated significantly better viability compared to control cultures. Sericin appeared to improve ARPE-19 maturation and morphology in vitro. No effects were seen on RPE pigmentation with the concentration of sericin and duration of cell culture herein reported. Conclusions This is the first study to demonstrate that supplementing the culture media with sericin promotes the viability of iPSC-RPE and ARPE-19. Sericin’s viability-promoting effects may have important implications for retinal therapeutics and regenerative medicine research.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::536ebad30d32c197c1e99bcdabe2f7aeTest
https://doi.org/10.1080/02713683.2020.1809001Test

المؤلفون: Henrik Holvin Jacobsen, Tiril Sandell, Are Hugo Pripp, Goran Petrovski, Øystein Kalsnes Jørstad, Per Kristian Eide, Morten Carsten Moe

المصدر: Translational Vision Science & Technology

مصطلحات موضوعية: Pseudotumor Cerebri, optical coherence tomography, Intracranial Pressure, integumentary system, medicine.diagnostic_test, Computer science, musculoskeletal, neural, and ocular physiology, Optic Disk, intracranial pressure, pulsatile pressure, noninvasive, Non invasive, Biomedical Engineering, Pulsatile flow, Article, humanities, nervous system diseases, Ophthalmology, Optical coherence tomography, medicine, Humans, Intracranial Hypertension, Tomography, Optical Coherence, Intracranial pressure, Biomedical engineering

الوصف: Purpose: To explore the ability of optical coherence tomography (OCT) to noninvasively estimate pulsatile and static intracranial pressure (ICP). Methods: An OCT examination was performed in patients who underwent continuous overnightmonitoring of the pulsatile and static ICP for diagnostic purpose. We included two patient groups, patients with idiopathic intracranial hypertension (IIH ; n = 20) and patients with no verified cerebrospinal fluid disturbances (reference ; n = 12). Several OCT parameters were acquired using spectral-domain OCT (RS-3000 Advance ; NIDEK, Singapore). The ICPmeasurementswere obtained using a parenchymal sensor (Codman ICP MicroSensor ; Johnson & Johnson, Raynham, MA, USA). The pulsatile ICP was determined as the mean ICPwave amplitude (MWA), and the static ICPwas determined as the mean ICP. Results: The peripapillary Bruch's membrane angle (pBA) and the optic nerve head height (ONHH) differed between the IIH and reference groups and correlated with both MWA and mean ICP. Both OCT parameters predicted elevated MWA. Area under the curve and cutoffs were 0.82 (95% confidence interval [CI], 0.66-0.98) and -0.65 degrees (sensitivity/specificity ; 0.75/0.92) for pBA and 0.84 (95% CI, 0.70-0.99) and 405 mu m (0.88/0.67) for ONHH. Adjusting for age and body mass index resulted in nonsignificant predictive values for mean ICP, whereas the predictive value for MWA remained significant. Conclusions: This study provides evidence that the OCT parameters pBA and ONHH noninvasively can predict elevated pulsatile ICP, represented by the MWA. Translational Relevance: OCT shows promise as a method for noninvasive estimation of ICP.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a340523f647a3a3510c7dcd2cf6d86eeTest
https://doi.org/10.21203/rs.3.rs-480386/v1Test

المؤلفون: Boris Malyugin, Ilias H. Sharafetdinov, Olav Kristianslund, Richárd Nagymihály, Morten Carsten Moe, Natalia S. Anisimova, Goran Petrovski, Jovana Bisevac, Agate Noer, Liv Drolsum, Kirankumar Katta

المصدر: Scientific Reports
Scientific Reports, Vol 10, Iss 1, Pp 1-17 (2020)

مصطلحات موضوعية: 0301 basic medicine, Male, genetic structures, medicine.medical_treatment, Intraocular lens, CXCR4, Extracellular matrix, 0302 clinical medicine, Cell Movement, Myofibroblasts, Cells, Cultured, Aged, 80 and over, Lenses, Intraocular, Transdifferentiation, Multidisciplinary, biology, Lens Subluxation, Extracellular Matrix, Medicine, Female, Collagen, Myofibroblast, medicine.medical_specialty, Intraocular Lens Dislocation, Epithelial-Mesenchymal Transition, Science, Pseudoexfoliation syndrome, Lens Capsule, Crystalline, Article, 03 medical and health sciences, Ophthalmology, medicine, Humans, Aged, Cell Proliferation, business.industry, Epithelial Cells, Translational research, medicine.disease, Crystallins, eye diseases, Fibronectin, 030104 developmental biology, Gene Expression Regulation, 030221 ophthalmology & optometry, biology.protein, sense organs, business, Ex vivo, Biomarkers

الوصف: Late spontaneous in-the-bag intraocular lens (IOL) dislocation is a complication presenting 6 months or later after cataract surgery. We aimed to characterize the cells in the lens capsules (LCs) of 18 patients with spontaneous late in-the-bag IOL dislocation. Patients' average age was 82.6 ± 1.5 years (range 72–98), and most of them had pseudoexfoliation syndrome (PEX). Cells from the LCs were positive for myofibroblast (αSMA), proliferation (Ki-67, PCNA), early lens development/lens progenitor (SOX2, PAX6), chemokine receptor (CXCR4), and transmembrane (N-cadherin) markers, while negative for epithelial (E-cadherin) marker. Moreover, the cells produced abundant fibronectin, type I and type V collagen in the nearby extracellular matrix (ECM). During ex vivo cultivation of dislocated IOL-LCs in toto, the cells proliferated and likely migrated onto the IOL’s anterior side. EdU proliferation assay confirmed the proliferation potential of the myofibroblasts (MFBs) in dislocated IOL-LCs. Primary cultured lens epithelial cells/MFBs isolated from the LC of dislocated IOLs could induce collagen matrix contraction and continuously proliferated, migrated, and induced ECM remodeling. Taken together, this indicates that long-lived MFBs of dislocated IOLs might contribute to the pathogenic mechanisms in late in-the-bag IOL dislocation.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7392c0e3517b53c4c02a330d74f55efdTest
http://europepmc.org/articles/PMC7689492Test

المؤلفون: Réka Albert, Lyubomyr Lytvynchuk, Morten Carsten Moe, Goran Petrovski, Richárd Nagymihály, Natasha Josifovska, Kai Kaarniranta, Zoltán Veréb

المصدر: International Journal of Molecular Sciences
Volume 21
Issue 3
International Journal of Molecular Sciences, Vol 21, Iss 3, p 813 (2020)

مصطلحات موضوعية: 0301 basic medicine, medicine.medical_treatment, Anti-Inflammatory Agents, retinal pigment epithelium, Vacuole, Resveratrol, resveratrol, lcsh:Chemistry, chemistry.chemical_compound, 0302 clinical medicine, lcsh:QH301-705.5, Spectroscopy, Cells, Cultured, protein array, medicine.diagnostic_test, Cell Death, General Medicine, Transfection, Computer Science Applications, Cell biology, Cytokine, Proteasome Endopeptidase Complex, autophagy, Cell Survival, survival, Catalysis, Article, Flow cytometry, Cell Line, Inorganic Chemistry, 03 medical and health sciences, medicine, Humans, Propidium iodide, Physical and Theoretical Chemistry, Molecular Biology, age-related macular degeneration, PI3K/AKT/mTOR pathway, proteasomal inhibition, Organic Chemistry, Autophagy, Epithelial Cells, eye diseases, 030104 developmental biology, chemistry, lcsh:Biology (General), lcsh:QD1-999, inflammation, 030221 ophthalmology & optometry, sense organs

الوصف: Purpose: To investigate the mechanism by which resveratrol acts upon retinal pigment epithelial (RPE) cells and to characterize its effect upon autophagy, survival, and inflammation, with consequent implications to treatment for age-related macular degeneration (AMD). Methods: Cultured ARPE-19 cells were exposed to 10 and 50 &mu
M resveratrol. Cell survival/death was determined by annexin-FITC/propidium iodide using flow cytometry, while autophagy was studied by detecting autophagic vacuoles formation (acridine orange and transmission electron microscopy), as well as LC3II/I ratio and p62 expression by Western blot. In addition, time-lapse confocal microscopy of a pDENDRA-LC3 expression vector was performed to detect autophagy in transfected ARPE-19 cells under the different treatment conditions. Inhibition of proteasomal and autophagy-lysosomal fusion was carried out by MG-132 and chloroquine, respectively, while induction of autophagy was achieved by rapamycin treatment. Detection of secreted cytokines by ARPE-19 cells using Human XL Cytokine Array was performed under oxidative stress (H2O2) and resveratrol treatments, respectively. Results: Resveratrol induced autophagy in ARPE-19 cells as determined by augmented presence of autophagic vacuoles, increased LC3II/I ratio and decreased p62 expression, as well as time-lapse confocal microscopy using pDENDRA-LC3 expression vector. Resveratrol acted similarly to proteasomal inhibition and downstream of mammalian target of rapamycin (mTOR), since upstream inhibition of autophagy by 3-methyladenine could not inhibit autophagy in ARPE-19 cells. Co-treatmeant by rapamycin and/or proteasome inhibition showed no additive effect upon autophagy induction. ARPE-19 cells treated by resveratrol showed lower cell death rate compared to untreated controls. Resveratrol induced a specific anti-inflammatory response in ARPE-19 cells. Conclusions: Resveratrol can induce autophagy, pro-survival, and anti-inflammatory stimuli in ARPE-19 cells, properties which could be plausible to formulate future treatment modalities for AMD.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5bb4bbf2ee61c8d9d444326b57fd022Test

المؤلفون: Martin Heier, Morten Carsten Moe, Hanna Dis Margeirsdottir, Aida Simeunovic, Naila Saddique, Knut Dahl-Jørgensen, Cathrine Brunborg, Goran Petrovski, Nina C.B.B. Veiby

المصدر: Journal of Diabetes Research, Vol 2020 (2020)
Journal of Diabetes Research

مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, Waist, Capillary plexus, Article Subject, Adolescent, Endocrinology, Diabetes and Metabolism, Risk Assessment, Diseases of the endocrine glands. Clinical endocrinology, Ophthalmoscopy, Young Adult, chemistry.chemical_compound, Endocrinology, Oct angiography, Predictive Value of Tests, Risk Factors, Ophthalmology, medicine, Humans, Macula Lutea, Type 1 diabetes, Diabetic Retinopathy, medicine.diagnostic_test, business.industry, Age Factors, Angiography, Retinal, Diabetic retinopathy, Foveal avascular zone, medicine.disease, RC648-665, eye diseases, Cross-Sectional Studies, Diabetes Mellitus, Type 1, Early Diagnosis, chemistry, Case-Control Studies, Female, business, Microvascular Density, Tomography, Optical Coherence, Research Article

الوصف: Background/Objective. Optical coherence tomography angiography (OCTA) is increasingly used to supplement ophthalmoscopy in the diagnosis and follow-up of diabetic retinopathy. Our objective was to confirm if OCTA parameters can predict the development of nonproliferative diabetic retinopathy (NPDR) and to clarify if any single OCTA parameter is associated with NPDR independently of well-known risk factors in young type 1 diabetes (T1D) patients. Methods. OCTA of both eyes was performed in a cross-sectional study of 14 to 30-year-old individuals with at least 10-year duration of T1D and controls recruited from the Norwegian Atherosclerosis and Childhood Diabetes (ACD) study. Vessel density (VD) and foveal avascular zone (FAZ) area in the superficial and deep capillary plexus (SCP and DCP), total retinal volume (TRV), and central macular thickness (CMT) were calculated using automated software. Univariate and multivariate ordered logistic regression (OLR) models were used accordingly. Results. We included 168 control eyes and 315 T1D eyes. Lower VD in DCP (OR 0.65, 95% CI 0.51–0.83), longer diabetes duration (OR 1.51, 95% CI 1.22–1.87), and higher waist circumference (OR 1.08, 95% CI 1.02–1.14) were significantly associated with progression of NPDR. VD in SCP and DCP were significantly lower in T1D patients without diabetic retinopathy than in controls. Conclusions. Sparser VD in DCP is significantly associated with severity of NPDR, supporting that OCTA might detect the earliest signs of NPDR before it is visible by ophthalmoscopy.

وصف الملف: text/xhtml

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2d691c71ca091dbeb1f347ab90c54d3Test
https://doaj.org/article/8c0f510063844e98823f9ec6aaac3d1eTest

المؤلفون: Algirdas Grevys, Bjørn Dalhus, Tilman Schlothauer, Malin C. Bern, Terje E. Michaelsen, Heidrun Elisabeth Lode, Inger Sandlie, Lene Støkken Høydahl, Mattia Ferrarese, Mirko Pinotti, Robert J. Davidson, John J Wilson, Kine Marita Knudsen Sand, Jan Terje Andersen, Rodney M. Camire, Gregory J. Christianson, Torleif Tollefsrud Gjølberg, Morten Carsten Moe, Silvia Lombardi, Espen S. Baekkevold, Derry C. Roopenian, Stian Foss, Alessio Branchini, Jeannette Nilsen

المساهمون: Bern, M, Nilsen, J, Ferrarese, M, Sand, K, Gjølberg, T, Lode, H, Davidson, R, Camire, R, Bækkevold, E, Foss, S, Grevys, A, Dalhus, B, Wilson, J, Høydahl, L, Christianson, G, Roopenian, D, Schlothauer, T, Michaelsen, T, Moe, M, Lombardi, S, Pinotti, M, Sandlie, I, Branchini, A, Terje Andersen, J

المصدر: 12:eabb0580
Science Translational Medicine

مصطلحات موضوعية: Genetically modified mouse, Recombinant Fusion Proteins, albumin, half-life, fcrn, fusion proteins, coagulation, Socio-culturale, Serum Albumin, Human, Receptors, Fc, Immunoglobulin G, law.invention, LS1_1, Neonatal Fc receptor, LS1_5, law, Albumins, transmucosal delivery, Human albumin, protein engineering, albumin fusion proteins, half-life prolongation, Transcellular, Biological Products, biology, Chemistry, Histocompatibility Antigens Class I, Albumin, General Medicine, Cell biology, Paracellular transport, biology.protein, Recombinant DNA, Nasal administration, Half-Life

الوصف: Needle-free uptake across mucosal barriers is a preferred route for delivery of biologics, but the efficiency of unassisted transmucosal transport is poor. To make administration and therapy efficient and convenient, strategies for the delivery of biologics must enhance both transcellular delivery and plasma half-life. We found that human albumin was transcytosed efficiently across polarized human epithelial cells by a mechanism that depends on the neonatal Fc receptor (FcRn). FcRn also transported immunoglobulin G, but twofold less than albumin. We therefore designed a human albumin variant, E505Q/T527M/K573P (QMP), with improved FcRn binding, resulting in enhanced transcellular transport upon intranasal delivery and extended plasma half-life of albumin in transgenic mice expressing human FcRn. When QMP was fused to recombinant activated coagulation factor VII, the half-life of the fusion molecule increased 3.6-fold compared with the wild-type human albumin fusion, without compromising the therapeutic properties of activated factor VII. Our findings highlight QMP as a suitable carrier of protein-based biologics that may enhance plasma half-life and delivery across mucosal barriers.

وصف الملف: STAMPA; application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24e3afbab5d3fd5d83466671e027f7c1Test
https://hdl.handle.net/10281/413558Test

المؤلفون: Morten Carsten Moe, Beáta Éva Petrovski, Ragnheidur Bragadottir, Ketil Eriksen, Goran Petrovski, Silvia Nanjala Walekhwa Hertzberg, Nina C.B.B. Veiby

المصدر: Acta ophthalmologicaReferences. 98(6)

مصطلحات موضوعية: Pars plana, Male, medicine.medical_specialty, Visual acuity, genetic structures, Cost effectiveness, medicine.medical_treatment, Cost-Benefit Analysis, Operative Time, Vitrectomy, 03 medical and health sciences, 0302 clinical medicine, Lens Implantation, Intraocular, medicine, Humans, Prospective Studies, Posterior Capsulotomy, Aged, Aged, 80 and over, Phacoemulsification, business.industry, Norway, Diagnosis-related group, General Medicine, Middle Aged, Surgery, Ophthalmology, medicine.anatomical_structure, 030221 ophthalmology & optometry, Capsulotomy, Female, Quality-Adjusted Life Years, medicine.symptom, business, 030217 neurology & neurosurgery, Pseudophakia

الوصف: Purpose To evaluate the cost‐effectiveness of the triple procedure (phacovitrectomy + posterior capsulotomy, PhacoPPVc) compared to the double‐ (phacovitrectomy, PhacoPPV) or single sequential procedures. Methods Prospective study on 31 eyes from 31 patients (mean age: 72.1 ± 9.1 years; 55% females) was performed with a preoperative decision to undergo only pars plana vitrectomy (PPV) (26%) or PhacoPPV (74%) and/or posterior capsulotomy based upon presence or absence of lens opacification or pseudophakia. Time during and between surgeries, surgical procedure codes, medical and transport costs, outcome and likelihood of complications after surgery were all included in the analysis. Societal perspectives and visual acuity were considered as measures of quality of adjusted life years (QALYs). Results About 23 eyes underwent triple procedure and eight eyes underwent vitrectomy only (mean surgery times: 35.9 and 24.0 min, respectively). Posterior capsulotomy took on average 30 s, while preparation and cataract procedure took 13.0 min. The patients travelled on average 80km (average cost: $280.12) to the surgery unit. The average reimbursement fee for the day procedures ranged between $174.17 (YAG capsulotomy; Diagnosis Related Group (DRG): 0.034), $1045.48 (Phaco + intraocular lens (IOL); DRG: 0.204) and $1701.32 (PPV; DRG: 0.332). The combined procedures excluded lens and laser reimbursements, while the calculated reimbursements for the double/triple procedures were $2713.08/$2901.45, respectively, without significant loss of QALYs. PhacoPPVc was found to be unequivocally cost‐effective, while PhacoPPV remained cost saving compared to sequential procedures. Conclusion This study confirms that the triple procedure has benefits to the patients, health institution and surgeon. For patients, it saves them travel and healing time; for health institution, it justifies the calculated higher costs and need for higher reimbursement for the double/triple procedures, which are cost saving.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2954bb349787b27e51c2cba65b251c9Test
https://pubmed.ncbi.nlm.nih.gov/32078246Test

المؤلفون: Morten Carsten Moe, Turid Heiberg, Torbjørn Wisløff, Elma Jelin

المصدر: Health and Quality of Life Outcomes
Health and Quality of Life Outcomes, Vol 17, Iss 1, Pp 1-9 (2019)

مصطلحات موضوعية: Male, Vascular Endothelial Growth Factor A, medicine.medical_specialty, Visual acuity, genetic structures, Intraclass correlation, Population, Visual Acuity, lcsh:Computer applications to medicine. Medical informatics, Macular Degeneration, Cronbach's alpha, Validation, medicine, Numeric Rating Scale, Humans, Translations, NEI-VFQ 25, education, Aged, Face validity, Aged, 80 and over, Intravitreal injections, education.field_of_study, Questionnaire, Norway, business.industry, Research, Age-related macular degeneration, Anti-VEGF, Public Health, Environmental and Occupational Health, Reproducibility of Results, General Medicine, Macular degeneration, medicine.disease, Patient reported outcome measures, eye diseases, Cross-Sectional Studies, Case-Control Studies, Quality of Life, Physical therapy, lcsh:R858-859.7, Female, Patient-reported outcome, medicine.symptom, business

الوصف: Background Although visual acuity and optical coherence tomography (OCT) are most widely used as outcomes in treatment of neovascular age-related Macular Degeneration (nAMD), patient reported outcome measures are increasingly recognized. National Eye Institute Visual Function Questionnaire (NEI-VFQ 25) was developed to capture the perceived visual function. Yet, evidence of psychometric performance in the target population is required. The aim of this study was to examine the psychometric properties of NEI-VFQ 25 in a Norwegian cohort of newly diagnosed nAMD patients followed with a Treat and Extend (T/E) protocol. Methods Patients receiving intravitreal anti–vascular endothelial growth factor (anti-VEGF) injection treatment according to a T/E protocol completed a Norwegian translation of NEI-VFQ 25, EuroQoL Health Questionnaire (EQ-5D), and Patient acceptable symptom state (PASS 5) at baseline, 3, 6 and 12 months. In addition, a control population completed the same questionnaires. Visual acuity was assessed with LogMar for best/treated eye. Validity testing comprised face validity by a 0–10 numeric rating scale about relevance of NEI-VFQ 25 as well as regression analyses and correlations between NEI-VFQ 25 and other relevant variables. Reliability was examined with Intraclass Correlation Coefficient (ICC) and Cronbach’s alpha for internal consistency were performed. Responsiveness, discriminatory power and predictive value were also explored. Results Number of respondents at baseline, after 3, 6 and 12 months was 197, 186, 176 and 168, respectively. The control population comprised 26 individuals. Face validity of NEI-VFQ 25 had a mean (SD) of 7.8 (1.7) (n = 84). NEI-VFQ was significantly correlated to visual acuity and PASS 5 as well as EQ-5D at baseline. Reliability (ICC) of the overall and sub scores for the patients/controls ranged from 0.49–0.97/0.59–0.97. Cronbach’s alpha was 0.61–0.85. Discriminatory power was confirmed by significant differences of the overall score between controls and patients (P

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::552f8386d3a06beb4b04d65852bc55cbTest
https://doi.org/10.1186/s12955-019-1203-0Test

المؤلفون: Erlend Christoffer Sommer Landsend, Øygunn Aass Utheim, Tor Paaske Utheim, Rigmor C. Baraas, Ragnheidur Bragadottir, Corina S. Rueegg, Neil Lagali, Hilde Røgeberg Pedersen, Morten Carsten Moe

المصدر: Investigative Ophthalmology and Visual Science

مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, congenital, hereditary, and neonatal diseases and abnormalities, Adolescent, genetic structures, Nystagmus, Fundus (eye), Young Adult, 03 medical and health sciences, 0302 clinical medicine, Optical coherence tomography, Foveal, Ophthalmology, medicine, Humans, Prospective Studies, Fluorescein Angiography, Child, Aniridia, 030304 developmental biology, 0303 health sciences, medicine.diagnostic_test, business.industry, Middle Aged, medicine.disease, Hypoplasia, eye diseases, 3. Good health, Scanning laser ophthalmoscopy, Ophthalmoscopy, Congenital Aniridia, Case-Control Studies, Oftalmologi, 030221 ophthalmology & optometry, Female, sense organs, aniridia, fundus autofluorescence, macular hypoplasia, optical coherence tomography, widefield imaging, medicine.symptom, business, Tomography, Optical Coherence

الوصف: PURPOSE. To investigate fundus autofluorescence (FAF) and other fundus manifestations in congenital aniridia. METHODS. Fourteen patients with congenital aniridia and 14 age- and sex-matched healthy controls were examined. FAF images were obtained with an ultra-widefield scanning laser ophthalmoscope. FAF intensity was quantified in the macular fovea and in a macular ring surrounding fovea and related to an internal reference within each image. All aniridia patients underwent an ophthalmologic examination, including optical coherence tomography and slitlamp biomicroscopy. RESULTS. Mean age was 28.4 +/- 15.0 years in both the aniridia and control groups. Fovea could be defined by subjective assessment of FAF images in three aniridia patients (21.4%) and in all controls (P = 0.001). Mean ratio between FAF intensity in the macular ring and fovea was 1.01 +/- 0.15 in aniridia versus 1.18 +/- 0.09 in controls (P = 0.034). In aniridia, presence of foveal hypoplasia evaluated by biomicroscopy correlated with lack of foveal appearance by subjective analyses of FAF images (P = 0.031) and observation of nystagmus (P = 0.009). CONCLUSIONS. Aniridia patients present a lower ratio between FAF intensity in the peripheral and central macula than do healthy individuals. Both subjective and objective analyses of FAF images are useful tools in evaluation of foveal hypoplasia in aniridia. Funding Agencies|Aniridia Norway (Oslo, Norway); Jon Larsen foundation (Tonsberg, Norway); Inger Holms memorial foundation (Oslo, Norway); Norwegian Association of the Blind and Partially Sighted (Oslo, Norway); Norwegian Ophthalmological Society (Oslo, Norway); Department of Ophthalmology at Oslo University Hospital (Oslo, Norway); National Centre for Optics, Vision and Eye Care at the University of South-Eastern Norway; European Union Seventh Framework Program (FP7-PEOPLE-2013-COFUND) [609020]

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f321a2808f2645524ad76d0326273ba5Test
http://hdl.handle.net/11250/2636461Test

المؤلفون: Geir Ringstad, Tiril Sandell, Per Kristian Eide, Morten Carsten Moe, Henrik Holvin Jacobsen, Øystein Kalsnes Jørstad

مصطلحات موضوعية: Adult, Male, 0301 basic medicine, Optic tract, genetic structures, Contrast Media, Optic chiasm, Visual system, Subarachnoid Space, Gadobutrol, 03 medical and health sciences, 0302 clinical medicine, Cerebrospinal fluid, Organometallic Compounds, medicine, Humans, Optic Tract, Visual Pathways, Prospective Studies, Injections, Spinal, Cerebrospinal Fluid, Visual Cortex, business.industry, Optic Nerve, Magnetic Resonance Imaging, 030104 developmental biology, medicine.anatomical_structure, Optic Chiasm, Optic nerve, Female, Glymphatic system, Subarachnoid space, business, Nuclear medicine, Glymphatic System, 030217 neurology & neurosurgery, medicine.drug

الوصف: Purpose: Explore in vivo whether there is direct communication between the cerebrospinal fluid (CSF) and extravascular compartment of human visual pathway structures. Methods: A prospective and observational study included 10 subjects who underwent intrathecal gadolinium–enhanced magnetic resonance imaging (MRI) for suspected CSF circulation disorder, but with a negative result and with no known ophthalmic diseases. After precontrast T1-weighted MRI, 0.5 mL of gadobutrol (Gadovist, 1.0 mmol/mL) was injected intrathecally. Gadobutrol distributes in the extravascular space, and served as a CSF tracer. Consecutive MRI scans were obtained throughout 24 to 48 hours. To assess gadobutrol contrast enrichment, regions of interest (ROIs) were placed at multiple locations along the visual pathway, from the primary visual cortex to the eye's vitreous body. CSF tracer dependent T1 signal was measured in each ROI. A linear mixed-model was used for statistical analyses. Results: CSF tracer enrichment was found within the optic nerve, optic chiasm, optic tract, and primary visual cortex (P < 0.001). Peak tracer enrichment in the visual pathway generally occurred after 24 hours and was preceded by peak enhancement in the prechiasmatic cistern after 4 to 6 hours. Conclusions: The results indicate direct communication between CSF of subarachnoid space and the extravascular space of the human visual pathway. Extravascular entry of the CSF tracer is a prerequisite for a glymphatic system, the present findings may suggest its presence. The existence of a glymphatic system in the human visual pathway could bring novel perspectives on the pathophysiology and treatment of ophthalmic diseases.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::159d481de2d74f0c1203f7bc5409fd80Test
http://hdl.handle.net/10852/75341Test