المؤلفون: Hamid Daneshvar, Mohammad Javad Namazi, Hossein Kamiabi, Richard Burchmore, Sarah Cleaveland, Stephen Phillips

المصدر: PLoS Neglected Tropical Diseases, Vol 9, Iss 5, p e0003787 (2015)

مصطلحات موضوعية: Arctic medicine. Tropical medicine, RC955-962, Public aspects of medicine, RA1-1270

وصف الملف: electronic resource

العلاقة: http://europepmc.org/articles/PMC4427506?pdf=renderTest; https://doaj.org/toc/1935-2727Test; https://doaj.org/toc/1935-2735Test

الوصول الحر: https://doaj.org/article/32d6314f470b4be58145a904c88e5b0aTest

المؤلفون: Hamid Daneshvar, Mohammad Javad Namazi, Hossein Kamiabi, Richard Burchmore, Sarah Cleaveland, Stephen Phillips

المصدر: PLoS Neglected Tropical Diseases, Vol 8, Iss 4, p e2757 (2014)

مصطلحات موضوعية: Arctic medicine. Tropical medicine, RC955-962, Public aspects of medicine, RA1-1270

الوصف: An attenuated line of Leishmania infantum (L. infantum H-line) has been established by culturing promastigotes in vitro under gentamicin pressure. A vaccine trial was conducted using 103 naive dogs from a leishmaniosis non-endemic area (55 vaccinated and 48 unvaccinated) brought into an endemic area of southeast Iran. No local and/or general indications of disease were observed in the vaccinated dogs immediately after vaccination. The efficacy of the vaccine was evaluated after 24 months (4 sandfly transmission seasons) by serological, parasitological analyses and clinical examination. In western blot analysis of antibodies to L. infantum antigens, sera from 10 out of 31 (32.2%) unvaccinated dogs, but none of the sera from vaccinated dogs which were seropositive at >100, recognized the 21 kDa antigen of L. infantum wild-type (WT). Nine out of 31 (29%) unvaccinated dogs, but none of vaccinated dogs, were positive for the presence of Leishmania DNA. One out of 46 (2.2%) vaccinated dogs and 9 out of 31 (29%) unvaccinated dogs developed clinical signs of disease. These results suggest that gentamicin-attenuated L. infantum induced a significant and strong protective effect against canine visceral leishmaniosis in the endemic area.

وصف الملف: electronic resource

العلاقة: http://europepmc.org/articles/PMC3990512?pdf=renderTest; https://doaj.org/toc/1935-2727Test; https://doaj.org/toc/1935-2735Test

الوصول الحر: https://doaj.org/article/dc3145c4fafb4dabace7f3db2ed9162dTest

المؤلفون: Farzad Mollahosseini, Mohammad Reza Hedayati-Moghaddam, Mohammad Javad Namazi, Hamid Reza Bidkhori, Narges Valizadeh, Houshang Rafatpanah, Arman Mosavat, Maryam Mahdifar, Seyed Abdolrahim Rezaee

المصدر: Journal of NeuroVirology. 26:863-869

مصطلحات موضوعية: Adult, CD4-Positive T-Lymphocytes, Male, 0301 basic medicine, Cellular immunity, Ki-1 Antigen, Iran, Antibodies, Viral, Lymphocyte Activation, Neopterin, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Immune system, immune system diseases, hemic and lymphatic diseases, Virology, Tropical spastic paraparesis, medicine, Humans, Human T cell lymphotropic virus type 1, Human T-lymphotropic virus 1, Immunity, Cellular, biology, business.industry, Interleukin-2 Receptor alpha Subunit, Antibody titer, virus diseases, Immunoglobulin E, Middle Aged, Viral Load, medicine.disease, HTLV-I Infections, Immunity, Innate, C-Reactive Protein, 030104 developmental biology, Neurology, Case-Control Studies, Asymptomatic Diseases, Immunology, Humoral immunity, biology.protein, Female, Neurology (clinical), Antibody, business, Asymptomatic carrier, 030217 neurology & neurosurgery

الوصف: It is estimated that about 10-20 million peoples are infected with human T-cell leukemia virus type 1 (HTLV-1) around the world and suffered from HTLV-related diseases. The present study was aimed to evaluate the cellular immunity, T-cell activation, humoral immunity, and inflammatory response hallmarks which affect HTLV-1-associated disease progression. A total of 78 participants were included in the study, comprising 39 HTLV-1 asymptomatic careers (ACs) and 39 healthy controls. The HTLV-proviral load (PVL) was determined via real-time PCR technique, and anti-HTLV antibody, sIL2R, sCD30, Neoptrin, hs-CRP, IgE, anti-VCA, anti-EBNA, and anti-EA were assessed by ELISA method. Mean PVL in ACs was 352.7 ± 418.7 copies/104 PBMCs. A significant higher level of sIL-2R was observed in ACs (P < 0.0001). Anti-VCA antibody titer in ACs and healthy controls was 80.72 ± 105.95 and 156.05 ± 130.71, respectively (P = 0.007). Intriguingly, suppression in ACs immune response was not observed. Resultantly, HTLV-1 infection has no effect on the humoral immune response in ACs but greater T-cell activation and function cellular responses were detected. Finally, more studies on various immune markers in adult T-cell leukemia/lymphoma (ATLL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients are greatly needed to illuminate the association of ACs' immune status with the development of the related diseases.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::983e40d58566dc048e35e984cb1e8330Test
https://doi.org/10.1007/s13365-020-00910-8Test

المؤلفون: Zahra Zamani, Bahman Rahimi Esboei, Mojtaba Didehdar, Mahdi Fakhar, Fatemeh Sadeghi, Aroona Chabra, Fateme Hajialiani, Taher Elmi, Mohammad Javad Namazi, Fatemeh Tabatabaie

المصدر: Acta Parasitologica. 66:39-52

مصطلحات موضوعية: medicine.drug_class, Plasmodium falciparum, Antiprotozoal Agents, medicine.disease_cause, Microbiology, Anti-Infective Agents, In vivo, Protozoan infection, parasitic diseases, Trichomonas vaginalis, medicine, Animals, Humans, Giardia lamblia, Chitosan, biology, technology, industry, and agriculture, Antimicrobial, medicine.disease, biology.organism_classification, Rats, Toxicity, Antiprotozoal, Parasitology

الوصف: Treatment of parasitic infections with conventional drugs is associated with high toxicity, and undesirable side effects require cogent substitutions. Nanotechnology has provided novel approaches to synthesize nano-drugs to improve efficient antipathetic treatment. Nano-chitosan as a nontoxic antimicrobial agent was examined against three most prevalent protozoa in humans, Plasmodium falciparum, Giardia lamblia and Trichomonas vaginalis. Chitosan extracted from Penicillium fungi was converted to nanoparticles to maximize its therapeutic properties. Safety of nano-chitosan was examined by determining its hemolytic property and toxicity on PC12 cells. The studied parasites were identified with RFLP-PCR and cultivation in relevant media. Characteristics of nano-chitosan as an useful and valuable curative compound was evaluated by FTIR, DLS and SEM. Dose dependent anti-parasitic effect of nano-chitosan was evaluated. The highest anti-parasitic activity of the nano-chitosan was observed at 50 μg/mL by which growth rates of cultivated P. falciparum, T. vaginalis and G. lamblia were inhibited by 59.5%, 99.4%, and 31.3%, respectively. The study demonstrated that nano-chitosan with the least toxicity, low side effects, and substantial efficacy deserved to be considered as an anti-parasitic nano-compound. Nano-chitosan significantly inhibited protozoan growth in vitro promising to explore its use to combat parasitic infections. Further investigations covering extended sample size, in vivo experiments and optimizing the concentration used may lead to efficient treatment of protozoan diseases.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76e853acf458bce361e92658a8d92997Test
https://doi.org/10.1007/s11686-020-00255-6Test

المؤلفون: Mohammad Javad Namazi, Mahtab Borji Esfahani, Taraneh Faghihi Langhroudi, Farbod Zahedi Tajrishi, Mohammadreza Naderian, Isa Khaheshi

المصدر: International Journal of Cardiovascular Practice. 4:89-93

مصطلحات موضوعية: medicine.medical_specialty, medicine.diagnostic_test, Emergency unit, business.industry, Complete blood count, General Medicine, Arterial obstruction, Helical ct, Pathophysiology, Internal medicine, Angiography, Cardiology, Medicine, In patient, Neutrophil to lymphocyte ratio, business

الوصف: Introduction: Acute Pulmonary thromboembolism (PTE) is an imperative medical condition with a considerable global impact. Inflammation is deemed to take a notable part in the pathophysiology of this potentially fatal disorder. The aim of the current study was to predict acute PTE severity in helical pulmonary CT-angiography using easily accessible hematological complete blood count (CBC) indices.Methods: After exclusion of inflammatory conditions that may affect CBC parameters, a total of 69 consecutive patients with definite diagnosis of acute PTE according to pulmonary helical CT –angiography were recruited. Laboratory tests, including CBC parameters were performed on admission in the emergency unit, before initiation of any therapy. Neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR) and RDW to platelet ratio (RPR) were calculated in each case of acute PTE. Ultimately, CT pulmonary arterial obstruction index (PAOI) was assessed subsequent to pulmonary helical CT-angiography for each patient.Results: We found that NLR is positively correlated with acute PTE severity according to CT pulmonary arterial obstruction index (PAOI) (P 0.05).Conclusions: NLR could be an easily calculated and capable index to predict severity of acute PTE in pulmonary CT-angiography. Consequently, NLR might be used in precise risk stratification when suspicious for acute PTE and in accurately triage of patients who would benefit greatly from urgent diagnostic and therapeutic interventions.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::89f3e9745c5f632af98b6a08bb041ed2Test
https://doi.org/10.29252/ijcp-27078Test

المؤلفون: Mohammad Javad Namazi, Hadi Atabati, Mohammad-Shafi Mojadadi, Ehsan Javaheri

المصدر: J Parasit Dis

مصطلحات موضوعية: Leishmania tropica, Leishmaniasis, Biology, medicine.disease, biology.organism_classification, Leishmania, Virology, Giemsa stain, High Resolution Melt, Cutaneous leishmaniasis, parasitic diseases, medicine, Parasite hosting, Parasitology, Leishmania major, Original Article

الوصف: Cutaneous leishmaniasis (CL) is a zoonotic disease with 1 to 1.5 million annual incidences. Microscopic examination of the Giemsa stained slides is the most common diagnostic method for CL. However, this method cannot distinguish leishmania species. Hence the present study was conducted to identify leishmania species by high-resolution melting (HRM) analysis in the newly emerged foci of CL in Sabzevar, northeast of Iran. In this cross-sectional study, fifty patients with suspicious cutaneous lesions referring to the designated health center for diagnosis and treatment of CL in Sabzevar during 2017–2018, were recruited. All collected samples and prepared slides were stained for microscopic examination and then undergone HRM real-time PCR (HRM-PCR) assay to identify species of Leishmania parasites. The results of HRM-PCR technique showed that Leishmania major (L. major) was the dominant causative parasite in the newly emerged foci whereas L. tropica (L. tropica) was positive only in two patients. This was the first time that 7SL RNA-HRM-PCR assay was performed to precisely identify leishmania parasites in the northeast of Iran. We proved the newfound foci in which both L. major and L. tropica were present. In contrast to the recent studies which identified only L. major in the region, we showed that L. tropica was still present.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::268ef19436f2026af1c3c6d60d3b879bTest
https://europepmc.org/articles/PMC7921233Test/

المؤلفون: Taher, Elmi, Fateme, Hajialiani, Mohamad Reza, Asadi, Sedigheh, Sadeghi, Mohammad Javad, Namazi, Fatemeh, Tabatabaie, Zahra, Zamani

المصدر: J Parasit Dis

مصطلحات موضوعية: Original Article

الوصف: Malaria still is the most fatal parasitic disease affecting 50% of the world's population. Although annual deaths attributed to malaria has reduced, crucial importance of its prevention and treatment remains a priority for health care systems and researchers. The worldwide increase in resistance to most common antimalarial drugs such as chloroquine, their unpleasant side effects and low efficiencies persuade researchers to prioritize finding alternative drugs including herbal medication from plant roots. The present study aimed to examine in vitro and in vivo effects of hydroalcoholic extract of herbal medicinal plant, Allium paradoxum, on growth rate in Plasmodium falciparum and Plasmodium berghei. The cytotoxicity assay was performed for hydroalcoholic extract of A. paradoxum. The 3D7 strain of P. falciparum was cultured. The IC(50) assay and enzymatic activity of lactate dehydrogenase were performed. BALB/c mice were infected with P. berghei in vivo. Toxicity and histopathological changes in the tissues of liver and kidney were also examined. The highest efficacy of A. paradoxum extract was observed at 80 μg/mL in P. falciparum culture resulting in 60.43% growth inhibition compared to control groups. The significantly highest parasite growth inhibition with 88.71% was seen in the mice infected with P. berghei when administered with 400 mg/kg extract compared to control groups. No significant changes in the liver and kidney cells were observed between experimental and control groups. The study showed that A. paradoxum extract exhibited significant antimalarial properties in vitro on P. falciparum and in vivo in mice infected with P. berghei. There was no significant toxicity in the liver and kidney of the treated mice.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::9a2fcab51252a66410bff54d211b6de3Test
https://pubmed.ncbi.nlm.nih.gov/34789989Test

المؤلفون: Parvaneh Rahimi-Moghaddam, Mohammad Javad Namazi, Abolghasem Siyadatpanah, Seyed Abdollah Hosseini, Fatemeh Tabatabaie, Rahim Golmohammadi, Saeideh Nikpour, Neda Sadat Shokouhi Mostafavi

المصدر: Infectious disorders drug targets. 21(3)

مصطلحات موضوعية: Microbiology (medical), Antioxidant, medicine.medical_treatment, chemistry.chemical_element, Calcium, medicine.disease_cause, BALB/c, Andrology, chemistry.chemical_compound, Mice, Selenium, medicine, Animals, Pharmacology, chemistry.chemical_classification, Mice, Inbred BALB C, biology, Glutathione peroxidase, Toxoplasma gondii, General Medicine, Glutathione, biology.organism_classification, chemistry, Dietary Supplements, Molecular Medicine, Toxoplasma, Oxidative stress

الوصف: Background: Studies have shown that selenium is an essential component of glutathione as an important antioxidant to reduce oxidative stress and inhibit intracellular parasites’ growth. In contrast, calcium in the cytosol of such parasites plays a key role in the entry of the parasite into the host cell and its primary motility. Aim and Objective: The present study was designed to evaluate and compare glutathione peroxidase bioactivity effects post administration of selenium and calcium in BALB/c mice infected by Toxoplasma gondii. Methods: Sixty BALB/c mice susceptible to T. gondii were randomly divided into twelve groups of case and control groups. There were six control groups including two positive controls infected only with the parasites either 104 or 5×104, non-infected and untreated groups. Treated controls received only calcium, selenium, or both respectively. Case groups were infected with 104 or 5×104 parasites. While each set of three case groups separately received minerals alone or together. Mice were orally fed with 200 μg selenium, 50 μg calcium or their combination for 7 days. Mice were infected by parasite’s tachyzoites. Sera of mice were kept and the peritoneal macrophages were isolated for counting tachyzoites during infection. Results: The results showed that selenium unlike calcium was significantly effective in reducing Toxoplasma tachyzoites compared to control groups. Moreover, glutathione peroxidase [GPX] activity was elevated in mice treated with selenium and vice versa decreased in mice treated with calcium. Conclusion: Administration of selenium unlike calcium reduced Toxoplasma tachyzoites proliferation by elevating bioactivity of selenium-dependent detoxification enzyme, GPX.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf2f5e4c7e58edbfbd93b6be952b6babTest
https://pubmed.ncbi.nlm.nih.gov/32416707Test

المؤلفون: H Moallaei, Hasan Rezaei Seresht, Maryam Latifnia, Mojtaba Fattahi Abdizadeh, Arash Ramezanpour Shahi, Bahareh Vahidiyanfar, Esrafil Mansouri, Mohammad Javad Namazi, Mahsa Daei Milani

المصدر: Journal of basic and clinical physiology and pharmacology.

مصطلحات موضوعية: medicine.medical_specialty, Physiology, Nephropathy, Diabetic nephropathy, Lipid peroxidation, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Internal medicine, Diabetes mellitus, Drug Discovery, medicine, Blood urea nitrogen, 030304 developmental biology, Pharmacology, chemistry.chemical_classification, 0303 health sciences, Creatinine, business.industry, Glutathione peroxidase, General Medicine, medicine.disease, Malondialdehyde, Endocrinology, chemistry, 030220 oncology & carcinogenesis, business

الوصف: Objectives The belief of therapeutic effects of herbal remedies in diseases such as diabetes is rooted in medical history. The present study evaluated protective efficacy of the hydroalcoholic extract of Vitex pseudo-negundo leaves (VLHE) on the renal disorders in streptozotocin-induced diabetic rats. Methods Fifty Wistar male rats were recruited and divided into five groups of 10, including healthy controls and diabetic controls: three diabetic groups of which first group was treated with glibenclamide, and two groups treated with 250 and 500 mg/kg of VLHE, respectively, for six weeks. Renal biochemical tests and tissue histopathological evaluation were performed and the antioxidant status was examined. Results There were significant decreases in superoxide dismutase and glutathione peroxidase activities and increases in malondialdehyde levels in renal tissue of diabetic groups compared with healthy controls. In the VLHE-treated rats, fasting blood sugar, blood urea nitrogen and creatinine were declined, serum albumin elevated, kidney weight lowered, lipid peroxidation and reinforcement of the activities of antioxidant enzymes decreased compared with healthy groups. Histological assessments revealed that the vacuolar degeneration of tubules and shrinkage of glomeruli in VLHE-treated rats was decreased compared with diabetic rats. Conclusions The study suggested that administrating of VLHE in nephropathic rats ameliorated the disease by reduction of oxidative stress and increase in renal antioxidant enzyme activities.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a368ad592b0baf7adb46f4f48573396Test
https://pubmed.ncbi.nlm.nih.gov/32776901Test

المؤلفون: Jean-Philippe Bouchara, Mohammad Javad Namazi, S. Pourhammed, H Moallaei

المساهمون: Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA)

المصدر: Journal de Mycologie Médicale
Journal de Mycologie Médicale, 2018, 28, pp.70-75. ⟨10.1016/j.mycmed.2017.12.004⟩

مصطلحات موضوعية: Male, 0301 basic medicine, Veterinary medicine, Adolescent, Universities, [SDV]Life Sciences [q-bio], education, 030106 microbiology, Direct examination, Dermatitis, Iran, Biology, DNA, Ribosomal, Polymerase Chain Reaction, law.invention, Young Adult, 03 medical and health sciences, Risk Factors, law, Tinea Versicolor, Prevalence, medicine, Humans, Students, Ribosomal DNA, Polymerase chain reaction, Skin, Cutaneous infections, Ribosomal, Malassezia, integumentary system, Direct microscopy, DNA, Pityriasis, medicine.disease, 3. Good health, Infectious Diseases, microscopy, Malassezia species, Female, Restriction fragment length polymorphism

الوصف: International audience; BACKGROUND: Malassezia species, usually part of normal human skin microbiota, may also cause cutaneous infections, mainly pityriasis versicolor (PV) which may rapidly spread in crowded communities, particularly in students' dormitories and sport leisure centers.OBJECTIVE: Few studies have been conducted on PV in students in the Middle East. The present study was designed to determine prevalence of Malassezia species and related diseases in students from city of Sabzevar, Northeast Iran.METHODS: Specimens were collected from 189students and analyzed by direct microscopy and cultures. Following PCR amplification of the large subunit of ribosomal DNA, species were identified by restriction fragment length polymorphism analysis (RFL-PCR).RESULTS: PV was suspected for 28students which was confirmed by direct examination and cultures. Cultures also revealed positive for 13students with healthy skin. Four Malassezia species were identified, with M.restricta as the most prevalent. A higher rate of PV was observed compared to other regions in Iran. However, despite the lipophilic feature of Malassezia species, no significant association was observed between PV or Malassezia species and fatty skin or gender.CONCLUSION: This study determined the frequencies of Malassezia species in part of Northeast Iran, but further studies are needed to identify risk factors for PV.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e8cf17d013541b7691aa48e924ed82bTest
https://doi.org/10.1016/j.mycmed.2017.12.004Test