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1دورية أكاديميةEfficacy of ear acupuncture on attention deficit / hyperactivity disorder in 7-14 years old children
المؤلفون: Farideh Farokhzadi, Afsaneh Khajevand Khosli, Mohamad Reza Mohamadi, Malahat Akbarfahimi, Neda Ali Beigi
المصدر: مجله دانشگاه علوم پزشکی گرگان, Vol 20, Iss 4, Pp 44-52 (2019)
مصطلحات موضوعية: Attention deficit / hyperactivity disorder, Ear-acupuncture, Child, Medicine, Medicine (General), R5-920
الوصف: Background and Objective: Attention deficit / hyperactivity disorder (ADHD) is one of the most common psychiatric disorders. This study was conducted to determine the effect of ear acupuncture on ADHD symptoms in children aged 7-14. Methods: This quasi-experimental study was performed on 30 children with ADHD whom did not receive Ritalin, whom referred to the private psychiatric clinic of children in Tehran. A questinare child syndrome inventory (parental form) and integrated visual and auditory (IVA) test with a pre-test and post-test design was used in this study. The ear acupuncture treatment for 8 weeks with uniform pressure on the ear seed was applied on five points of the ear, including brain, excitement, prefrontal cortex, shen men and tranquilizar. Results: The auditory vigilance subscale from the attention area and the auditory prudence subscale of the auditory response from the inhibition area in the pre-test phase in compared to the post-test were statistically significant (P
وصف الملف: electronic resource
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2دورية أكاديمية
المؤلفون: Farideh Farokhzadi, Mohamad Reza Mohamadi, Afsaneh Khajevand Khosli, Malahat Akbarfahimi, Neda Ali Beigi, Pegah Torabi
المصدر: Acta Medica Iranica, Vol 58, Iss 12 (2021)
مصطلحات موضوعية: Attention deficit-hyperactive disorder, Transcranial alternating current stimulation (TACS), Ritalin, Medicine (General), R5-920
الوصف: Several studies have been conducted on the effect of transcranial direct current stimulation on adult patients. But, in recent years, only a few studies have been carried out in children and teenagers because the aim of the present study was to compare the effectiveness of TACS and Ritalin in the treatment of attention deficit hyperactivity disorder (ADHD) symptoms. This interventional clinical trial study was performed on 62 children with ADHD who were referred to the private psychiatric clinic of children in Tehran. The children were randomly assigned to two coded groups based on a lottery so that they were enrolled in the TACS or the Ritalin group. A questionnaire child syndrome inventory (parental form) and integrated visual and auditory (IVA) test with a pretest and posttest design was used in this study. TACS therapy protocol was employed (3 days a week for eight weeks using alternating current stimulation at 10 Hz over two points on the prefrontal cortex: the anode centered over F3 [the left dorsolateral prefrontal cortex] and the cathode over F4[the right dorsolateral prefrontal cortex]). Results showed that the posttest scores of the TACS-treated group were higher than those of the Ritalin-treated group, and there was a significant difference between the areas of visual attention (visual vigilance, visual focus, Sustained attention visual) and response control visual and auditory prudence (P
وصف الملف: electronic resource
العلاقة: https://acta.tums.ac.ir/index.php/acta/article/view/8394Test; https://doaj.org/toc/0044-6025Test; https://doaj.org/toc/1735-9694Test
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3دورية أكاديمية
المؤلفون: Jonas MELIN, Keiko YAMADA, Laili MAHMOUDIAN, Manabu TOKESHI, Mats NILSSON, Michio OHTA, Mohamad Reza MOHAMADI, Noritada KAJI, Yoshinobu BABA
المصدر: Analytical Sciences. 2008, 24(3):327
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المؤلفون: Malahat Akbarfahim, Afsaneh Khajevand Khosli, Farideh Farokhzadi, Neda Ali Beigi, Pegah Torabi, Mohamad Reza Mohamadi
المصدر: Acta Medica Iranica, Vol 58, Iss 12 (2021)
مصطلحات موضوعية: Medicine (General), medicine.medical_specialty, business.industry, Ritalin, General Medicine, Audiology, medicine.disease, R5-920, Attention deficit-hyperactive disorder, medicine, Attention deficit hyperactivity disorder, Transcranial alternating current stimulation (TACS), business, Transcranial alternating current stimulation
الوصف: Several studies have been conducted on the effect of transcranial direct current stimulation on adult patients. But, in recent years, only a few studies have been carried out in children and teenagers because the aim of the present study was to compare the effectiveness of TACS and Ritalin in the treatment of attention deficit hyperactivity disorder (ADHD) symptoms. This interventional clinical trial study was performed on 62 children with ADHD who were referred to the private psychiatric clinic of children in Tehran. The children were randomly assigned to two coded groups based on a lottery so that they were enrolled in the TACS or the Ritalin group. A questionnaire child syndrome inventory (parental form) and integrated visual and auditory (IVA) test with a pretest and posttest design was used in this study. TACS therapy protocol was employed (3 days a week for eight weeks using alternating current stimulation at 10 Hz over two points on the prefrontal cortex: the anode centered over F3 [the left dorsolateral prefrontal cortex] and the cathode over F4[the right dorsolateral prefrontal cortex]). Results showed that the posttest scores of the TACS-treated group were higher than those of the Ritalin-treated group, and there was a significant difference between the areas of visual attention (visual vigilance, visual focus, Sustained attention visual) and response control visual and auditory prudence (P
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17c4e276080a922adf7e8c64b3df3c21Test
https://doi.org/10.18502/acta.v58i12.5156Test -
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المصدر: Methods in Molecular Biology ISBN: 9781493987924
Methods in Molecular Biology ISBN: 9781617798207مصطلحات موضوعية: Gel electrophoresis, biology, Chemistry, 010401 analytical chemistry, 02 engineering and technology, 021001 nanoscience & nanotechnology, Molecular biology, 01 natural sciences, 0104 chemical sciences, Cerebrospinal fluid, Microfluidic chip, Microchip Electrophoresis, biology.protein, Antibody, 0210 nano-technology
الوصف: Aggregation of beta-amyloid peptides especially Aβ1-42 in amyloid plaques is one of the major neuropathological events in Alzheimer's disease. This event is normally accompanied by a relative reduction of the concentration of Aβ1-42 in the cerebrospinal fluid (CSF) of patient developing the signs of Alzheimer's disease. Here, we describe methods for isolation and for microchip gel electrophoresis of Aβ peptides in polydimethylsiloxane (PDMS) microfluidic chip. The method was applied to compare the relative concentration of Aβ1-42 with other Aβ peptides, for example, Aβ 1-40 in CSF. In order to increase the sensitivity of detection, Aβ peptides in the CSF samples were first captured and concentrated using magnetic beads coated with specific anti-Aβ antibodies.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a52cdbde6bab10f911110d28cd9b0188Test
https://doi.org/10.1007/978-1-4939-8793-1_28Test -
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المؤلفون: Stefan Lehnert, Viovy Jean-Louis, Hans Klafki, Hermann Esselmann, Romain Verpillot, Florence Poirier, Jens Wiltfang, Myriam Taverna, Mohamad Reza Mohamadi, Markus Otto
المصدر: Analytical Chemistry. 83:1696-1703
مصطلحات موضوعية: chemistry.chemical_classification, Detection limit, Amyloid beta-Peptides, Chromatography, Chemistry, Lysine, Medizin, Fluorescence spectrometry, Electrophoresis, Capillary, Peptide, Context (language use), Analytical Chemistry, Matrix-assisted laser desorption/ionization, Capillary electrophoresis, Case-Control Studies, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Calibration, Electrophoresis, Polyacrylamide Gel, Peptides, Polyacrylamide gel electrophoresis
الوصف: We report a CE-LIF method for the separation and detection of five synthetic amyloid-β peptides corresponding to an important family of CSF-biomarkers in the context of Alzheimer disease (AD). The presumed most relevant peptides (Aβ1-42, Aβ1-40, and Aβ1-38) that may support the differentiation between AD and healthy patients or other dementias were successfully detected in CSF by incorporating an immunoconcentration step prior to CE analysis of derivatized peptides. We labeled the Aβ peptides with a fluoroprobe dye before CE-LIF analysis. This reagent reacts with the amino groups of lysine residues and produced mostly ditagged Aβ peptides under the proposed experimental conditions. The labeling reaction displayed similar efficiency with each one of the five different synthetic Aβ peptides that were tested. The limit of detection of the CE-LIF method approached 280 attomoles of injected synthetic labeled Aβ peptides. We obtained excellent correlation between peak areas and peptide concentrations from 35 nM to 750 nM. For the detection of Aβ peptides in human CSF samples, we enriched the peptides by immunoprecipitation prior to the CE-LIF analysis. The comparison of the CE-LIF profiles obtained from CSF samples from 3 AD patients and 4 non-demented control subjects indicated noticeable differences, suggesting that this method, which relies on a multibiomarker approach, may have potential as a clinical diagnostic test for AD.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6ae450d540d4a076d87c0007b022d7dTest
https://doi.org/10.1021/ac102828fTest -
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المؤلفون: Keiko Yamada, Yoshinobu Baba, Mats Nilsson, Jonas Melin, Noritada Kaji, Michio Ohta, Manabu Tokeshi, Laili Mahmoudian, Mohamad Reza Mohamadi
المصدر: Analytical Sciences. 24:327-332
مصطلحات موضوعية: DNA, Bacterial, Time Factors, medicine.disease_cause, Sensitivity and Specificity, Analytical Chemistry, Bacterial genetics, Electrophoresis, Microchip, chemistry.chemical_compound, medicine, Vibrio cholerae, Gene, biology, Chemistry, Reproducibility of Results, Pathogenic bacteria, biology.organism_classification, Molecular biology, Vibrio, Electrophoresis, genomic DNA, Biochemistry, Microchip Electrophoresis, DNA, Circular, Nucleic Acid Amplification Techniques, DNA
الوصف: We have developed a new method for a fast and precise analysis of circle-to-circle amplification (C2CA) product for specific gene detection by microchip electrophoresis. In this method, we have added a new enzymatic step to the C2CA reaction, which could be carried out isothermally at 37 degrees C. Compared to the original single-stranded DNA, the double-stranded DNA that is produced by this enzymatic reaction is more reliable for analysis by microchip electrophoresis. C2CA product was detected within 55 s with high reproducibility by this method which was successfully applied to the detection of 10-ng genomic DNA of the pathogenic bacteria Vibrio. cholerae within 110 s. Purification was found to be an indispensable step for the analysis of the C2CA product of genomic DNA samples.
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb79c0b61c2bf0a374c51c77c327382dTest
https://doi.org/10.2116/analsci.24.327Test -
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المؤلفون: Yoshinobu Baba, Manabu Tokeshi, Noritada Kaji, Mohamad Reza Mohamadi
المصدر: Analytical Chemistry. 80:312-316
مصطلحات موضوعية: Protein Denaturation, Hydrodynamic radius, Magnesium Chloride, Analytical chemistry, Electrophoretic Mobility Shift Assay, macromolecular substances, Analytical Chemistry, Ion, Electrophoresis, Microchip, Viscosity, chemistry.chemical_compound, Dynamic light scattering, Polymer chemistry, Humans, Methyl methacrylate, Magnesium ion, Serum Albumin, Carbonic Anhydrases, chemistry.chemical_classification, Chemistry, technology, industry, and agriculture, Povidone, Proteins, Polymer, Solutions, Electrophoresis, Cross-Linking Reagents, Lactalbumin, Glucan 1,4-alpha-Glucosidase, Trypsin Inhibitors
الوصف: We report a dynamic cross-linking effect of Mg2+ that enhances the sieving properties of low-viscosity poly(vinylpyrrolidone) (PVP) solutions. A low-viscosity PVP solution was applied to nondenaturing microchip electrophoresis of protein samples using microchips made of poly(methyl methacrylate). The separation resolution of nondenatured protein markers in 1.8% PVP solution was improved by adding 1-20 mM MgCl2. We studied the effect of the ratio of cross-linking agent on mobility of protein samples and showed that protein retardation (ln micro/micro0) is correlated with the ratio of cross-linking agent to PVP ([cMg2+/cPVP]) as ln micro/micro0=A'[cMg2+/cPVP]b'. A' was related to the protein radius (R), and b' was found to be 0.72 for proteins with R=2.4 nm and 0.82 for proteins with R=1.85 nm. A structural study of PVP in semidilute solutions using dynamic light scattering showed that incremental increases of Mg2+ ion concentration from 5 to 20 mM in 1.8% PVP solution increased the hydrodynamic radius of PVP polymers by 20%.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a20d64d23a87248c10fcff28275888c4Test
https://doi.org/10.1021/ac701974uTest -
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المؤلفون: Noritada Kaji, Mohamad Reza Mohamadi, Manabu Tokeshi, Yoshinobu Baba
المصدر: Analytical Chemistry. 79:3667-3672
مصطلحات موضوعية: Polymers, Serum albumin, Fluorescence spectrometry, Antigen-Antibody Complex, Analytical Chemistry, Electrophoresis, Microchip, chemistry.chemical_compound, Capillary electrophoresis, medicine, Humans, Polymethyl Methacrylate, Methyl methacrylate, Serum Albumin, Immunoassay, Chromatography, biology, medicine.diagnostic_test, Chemistry, Antibodies, Monoclonal, Human serum albumin, Poly(methyl methacrylate), body regions, visual_art, embryonic structures, visual_art.visual_art_medium, biology.protein, Isotachophoresis, medicine.drug
الوصف: Online preconcentration of human serum albumin (HSA) and its immunocomplex with a monoclonal antibody by on-chip transient isotachophoresis is reported. An 800-fold signal enhancement was achieved following the preconcentration on standard cross-channel microchips made of poly (methyl methacrylate). Sample injection, preconcentration, and separation were done continuously and controlled solely by a sequential voltage switching program. The preconcentration was followed by on-chip nondenaturing gel electrophoresis in methylcellulose solution. The method was applied to microchip electrophoresis immunoassay of HSA. Baseline separation of HSA and its immunocomplex was achieved in 25 s in the first 1 cm of the microchannel. In a direct immunoassay, the minimum detectable concentration of fluorescent labeled HSA by laser-induced fluorescence detection was 7.5 pM.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3251f640f50d0763539b281e4d4318f8Test
https://doi.org/10.1021/ac0623890Test -
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المصدر: ELECTROPHORESIS. 28:830-836
مصطلحات موضوعية: Surface Properties, Clinical Biochemistry, Polysorbates, Buffers, Methylcellulose, engineering.material, Biochemistry, Analytical Chemistry, Electrophoresis, Microchip, chemistry.chemical_compound, Coating, Animals, Polymethyl Methacrylate, Methyl methacrylate, Solubility, Chromatography, Microchannel, Serum Albumin, Bovine, Gel electrophoresis of proteins, Electrophoresis, chemistry, engineering, Cattle, Polysorbate 20, Adsorption, Glucan 1,4-alpha-Glucosidase, Trypsin Inhibitors, Protein adsorption
الوصف: A dynamic coating using methylcellulose (MC) and a nonionic detergent (polysorbate 20) was developed, which controlled protein adsorption onto the surface of microchannels on a microchip made of poly(methyl methacrylate) (PMMA). Optimum concentration of polysorbate 20 in combination with the range of MC concentrations controlled the protein adsorption onto the microchannel surface, and increased the solubility of the protein samples while facilitating the injection of high concentrations of MC solutions into the microchannels. Higher concentrations of nonionic detergent increased the EOF mobility as opposed to the electrophoretic mobility and caused the electrophoresis to fail. Nondenaturing microchip electrophoresis of protein samples with molecular masses ranging from 20 to 100 kDa were completed in 100 s. Also, successful separation of a BSA sample and its complex with anti-BSA mAb ( 220 kDa) was achieved on a PMMA microchip. The separation exhibited high reproducibility in both migration time (RSD = 1%) and peak area (RSD = 10-15%).
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::724618d04ca6ad9078513b9e43cc75a7Test
https://doi.org/10.1002/elps.200600373Test
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