المؤلفون: Siyuan Chang, Mingyang Chang, Gang Liu, Daqian Xu, Haili Wang, Rongqing Sun, Min Feng

المصدر: Biomedical Journal, Vol 45, Iss 4, Pp 642-653 (2022)

مصطلحات موضوعية: OIP5-AS1, miR-144-5p, PKM2, AKI, Cell viability, Apoptosis, Medicine (General), R5-920, Biology (General), QH301-705.5

الوصف: Background: The abnormal expression of long non-coding RNA (lncRNA) Opa-interacting protein 5 antisense RNA 1 (OIP5-AS1) has been observed in many human cancers and the underlying mechanisms have been well studied. However, the function of OIP5-AS1 in acute kidney injury (AKI) remains unclear. Methods: To explore the role of OIP5-AS1 in the progression of AKI, the cisplatin-induced AKI mouse and cell model were established. To confirm the potential protective effect of OIP5-AS1 during cisplatin-induced AKI, rescue experiments were performed. Targetscan was used to predict the potential targets of miR-144-5p. To further determine whether the effect of miR-144-5p during cisplatin-induced AKI was mediated by PMK2, the recuse experiments using PMK2 overexpressing vector was applied. Results: OIP5-AS1 was significantly downregulated both in cisplatin-induced AKI mice and human renal tubular cell line HK-2 cells. Moreover, overexpression of OIP5-AS1 efficiently promoted cell growth and reduced cisplatin-induced apoptosis of HK-2 cells. Furthermore, OIP5-AS1 was identified as a sponge of miR-144-5p, and upregulation of miR-144-5p could significantly reverse overexpression of OIP5-AS1-induced protective effect on the damage of cisplatin to HK-2 cells. In addition, pyruvate kinase M2 (PKM2) was found to be a direct target of miR-144-5p, and overexpression of PKM2 efficiently reversed the effect of miR-144-5p mimics on the damage in cisplatin-stimulated HK-2 cells. Conclusions: OIP5-AS1 reduced the apoptosis of cisplatin-stimulated renal epithelial cells by targeting the miR-144-5p/PKM2 axis, which extended the regulatory network of lncRNAs in cisplatin-induced AKI and also provided a novel therapeutic target for AKI treatment.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2319417021000962Test; https://doaj.org/toc/2319-4170Test

الوصول الحر: https://doaj.org/article/de460044e6a24ec0b6f4a459b0cd23ecTest

المؤلفون: Mingyang Chang, Rui Deng, Haijiang Wang, Bibo Xie, Zhongliang Zhao

المصدر: Applied Mathematics and Nonlinear Sciences, Vol 6, Iss 2, Pp 99-110 (2021)

مصطلحات موضوعية: logging curve, normalisation, xj oilfield, frequency histogram, mean variance robot, Mathematics, QA1-939

الوصف: During the long-term exploration and development of the oilfield, it is difficult to ensure that all well logging curves are measured by the same type of instrument, the same calibration standard and the same operation mode, For different wells, there must be systematic errors caused by these reasons. Therefore, in addition to environmental correction, it is necessary to standardise logging curves. In XJ oilfield, three logging companies use wireline logging and logging while drilling to complete logging, in multi-well logging interpretation. To eliminate the systematic errors of different measuring tools, to maximise the geological information reflected by logging curves, and to make logging interpretation follow the same standard as much as possible, it is necessary to standardise the logging curve in the whole oilfield. This article takes the standardisation of well 106 in XJ oilfield as an example, the standardisation of different methods was compared, the method of combining frequency histogram and mean variance is better.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2444-8656Test

الوصول الحر: https://doaj.org/article/82ae093397ce4d7d995b38aa5efb4a66Test

المؤلفون: Xuan Wang, Shuyong Wang, Baolin Guo, Yuxin Su, Zuolong Tan, Mingyang Chang, Jinmei Diao, Yi Zhao, Yunfang Wang

المصدر: Cell Death and Disease, Vol 12, Iss 1, Pp 1-17 (2021)

مصطلحات موضوعية: Cytology, QH573-671

الوصف: Abstract Technology of generating human epidermal derivatives with physiological relevance to in vivo epidermis is continuously investigated for improving their effects on modeling of human natural dermatological status in basic and clinical studies. Here, we report a method of robust establishment and expansion of human primary epidermal organoids (hPEOs) under a chemically defined condition. hPEOs reconstruct morphological, molecular, and functional features of human epidermis and can expand for 6 weeks. Remarkably, hPEOs are permissive for dermatophyte infections caused by Trichophyton Rubrum (T. rubrum). The T. rubrum infections on hPEOs reflect many aspects of known clinical pathological reactions and reveal that the repression on IL-1 signaling may contribute to chronic and recurrent infections with the slight inflammation caused by T. rubrum in human skin. Thus, our present study provides a new insight into the pathogenesis of T. rubrum infections and indicates that hPEOs are a potential ex vivo model for both basic studies of skin diseases and clinical studies of testing potential antifungal drugs.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2041-4889Test

الوصول الحر: https://doaj.org/article/8ea5c3521b4f4e7f99ce6d0f028d88e6Test

المؤلفون: Xiaofang Liu, Jinhua Qin, Mingyang Chang, Shuyong Wang, Yali Li, Xuetao Pei, Yunfang Wang

المصدر: Stem Cell Research & Therapy, Vol 11, Iss 1, Pp 1-12 (2020)

مصطلحات موضوعية: hPSCs, β cells, Three-dimensional culture, Differentiation, FAK, Connexin 36, Medicine (General), R5-920, Biochemistry, QD415-436

الوصف: Abstract Background Generation of insulin-producing cells from human pluripotent stem cells (hPSCs) in vitro would be useful for drug discovery and cell therapy in diabetes. Three-dimensional (3D) culture is important for the acquisition of mature insulin-producing cells from hPSCs, but the mechanism by which it promotes β cell maturation is poorly understood. Methods We established a stepwise method to induce high-efficiency differentiation of human embryonic stem cells (hESCs) into mature monohormonal pancreatic endocrine cells (PECs), with the last maturation stage in 3D culture. To comprehensively compare two-dimensional (2D) and 3D cultures, we examined gene expression, pancreas-specific markers, and functional characteristics in 2D culture-induced PECs and 3D culture-induced PECs. The mechanisms were considered from the perspectives of cell–cell and cell–extracellular matrix interactions which are fundamentally different between 2D and 3D cultures. Results The expression of the pancreatic endocrine-specific transcription factors PDX1, NKX6.1, NGN3, ISL1, and PAX6 and the hormones INS, GCG, and SST was significantly increased in 3D culture-induced PECs. 3D culture yielded monohormonal endocrine cells, while 2D culture-induced PECs co-expressed INS and GCG or INS and SST or even expressed all three hormones. We found that focal adhesion kinase (FAK) phosphorylation was significantly downregulated in 3D culture-induced PECs, and treatment with the selective FAK inhibitor PF-228 improved the expression of β cell-specific transcription factors in 2D culture-induced PECs. We further demonstrated that 3D culture may promote endocrine commitment by limiting FAK-dependent activation of the SMAD2/3 pathway. Moreover, the expression of the gap junction protein Connexin 36 was much higher in 3D culture-induced PECs than in 2D culture-induced PECs, and inhibition of the FAK pathway in 2D culture increased Connexin 36 expression. Conclusion We developed a strategy to induce differentiation of monohormonal mature PECs from hPSCs and found limited FAK-dependent activation of the SMAD2/3 pathway and unregulated expression of Connexin 36 in 3D culture-induced PECs. This study has important implications for the generation of mature, functional β cells for drug discovery and cell transplantation therapy for diabetes and sheds new light on the signaling events that regulate endocrine specification.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s13287-020-02003-zTest; https://doaj.org/toc/1757-6512Test

الوصول الحر: https://doaj.org/article/86771ca499ba43f5a8ac7c9495cf15c8Test

المؤلفون: Mingyang Chang, Bohan Chen, James Shaffner, Lance D. Dworkin, Rujun Gong

المصدر: Frontiers in Physiology, Vol 12 (2021)

مصطلحات موضوعية: melanocyte-stimulating hormone, adrenocorticotropic hormone, pro-opiomelanocortin, glomerulus, kidney disease, melanocortin receptors, Physiology, QP1-981

الوصف: Melanocortin peptides, melanocortin receptors, melanocortin receptor accessory proteins, and endogenous antagonists of melanocortin receptors are the key components constituting the melanocortin hormone system, one of the most complex and important hormonal systems in our body. A plethora of evidence suggests that melanocortins possess a protective activity in a variety of kidney diseases in both rodent models and human patients. In particular, the steroidogenic melanocortin peptide adrenocorticotropic hormone (ACTH), has been shown to exert a beneficial effect in a number of kidney diseases, possibly via a mechanism independent of its steroidogenic activity. In patients with steroid-resistant nephrotic glomerulopathy, ACTH monotherapy is still effective in inducing proteinuria remission. This has inspired research on potential implications of the melanocortin system in glomerular diseases. However, our understanding of the role of the melanocortinergic pathway in kidney disease is very limited, and there are still huge unknowns to be explored. The most controversial among these is the identification of effector cells in the kidney as well as the melanocortin receptors responsible for conveying the renoprotective action. This review article introduces the melanocortin hormone system, summarizes the existing evidence for the expression of melanocortin receptors in the kidney, and evaluates the potential strategy of melanocortin therapy for kidney disease.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fphys.2021.651236/fullTest; https://doaj.org/toc/1664-042XTest

الوصول الحر: https://doaj.org/article/945eed71087645258b66e291a06d0e6eTest

المؤلفون: Ling Leng, Jie Ma, Xuer Sun, Baolin Guo, Fanlu Li, Wei Zhang, Mingyang Chang, Jinmei Diao, Yi Wang, Wenjuan Wang, Shuyong Wang, Yunping Zhu, Fuchu He, Lola M Reid, Yunfang Wang

المصدر: Journal of Tissue Engineering, Vol 11 (2020)

مصطلحات موضوعية: Biochemistry, QD415-436

الوصف: Biomaterial scaffolds are increasingly being used to drive tissue regeneration. The limited success so far in human tissues rebuilding and therapy application may be due to inadequacy of the functionality biomaterial scaffold. We developed a new decellularized method to obtain complete anatomical skin biomatrix scaffold in situ with extracellular matrix (ECM) architecture preserved, in this study. We described a skin scaffold map by integrated proteomics and systematically analyzed the interaction between ECM proteins and epidermal cells in skin microenvironment on this basis. They were used to quantify structure and function of the skin’s Matrisome, comprised of core ECM components and ECM-associated soluble signals that are key regulators of epidermal development. We especially revealed that ECM played a role in determining the fate of epidermal stem cells through hemidesmosome components. These concepts not only bring us a new understanding of the role of the skin ECM niche, they also provide an attractive combinational strategy based on tissue engineering principles with skin biomatrix scaffold materials for the acceleration and enhancement of tissue regeneration.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2041-7314Test

الوصول الحر: https://doaj.org/article/ec362197aebd4d7ab5c83f90e2a750e8Test

المؤلفون: Xijin Zhang, Dan Wu, Qingyao Li, Faguang Pu, Xingli Xia, Mingyang Chang, Kun Song

المصدر: Forests, Vol 13, Iss 2, p 169 (2022)

مصطلحات موضوعية: cold temperature, non-structural carbohydrate (NSC), soluble sugar, starch, stem, Plant ecology, QK900-989

الوصف: Parenchyma in the secondary xylem comprises the main tissue for the storage of non-structural carbohydrates (NSC) in woody plants. Across species, the amount of parenchyma depends on the general environment of the distribution area and determines to a large extent the NSC storage. However, little information is available on the relationship between parenchyma fractions, NSC storage, and the environmental influences within individual species. This information is crucial to assessing the adaptive capacities of tree populations in the context of increasing the frequency and severity of stress-inducing events. In this study, parenchyma fractions and NSC concentrations of the secondary xylem in trunks of a subtropical evergreen oak (Quercus ciliaris C.C.Huang & Y.T.Chang) were quantified along an elevational gradient from 700 m to 1200 m a.s.l. in eastern China. Air temperatures within the distribution area correlated with altitude were recorded. The results showed that the total parenchyma fractions did not covary with the colder temperatures. However, axial parenchyma fractions were lower with a colder climate, while the fractions of multiseriate rays and total ray parenchyma were higher. Higher concentrations of starch and NSC were significantly associated with larger axial parenchyma fractions. The sugar concentration displayed no significant relationship with parenchyma fractions. These findings suggest that the total parenchyma fractions in secondary xylem do not increase in response to a colder climate, while colder temperatures drive changes in the composition of parenchyma for Q. ciliaris.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1999-4907/13/2/169Test; https://doaj.org/toc/1999-4907Test

الوصول الحر: https://doaj.org/article/5d721483c3804e27b2a8ceee5aba065fTest

المؤلفون: Mingyang Chang, Juan Liu, Baolin Guo, Xin Fang, Yi Wang, Shuyong Wang, Xiaofang Liu, Lola M. Reid, Yunfang Wang

المصدر: Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)

مصطلحات موضوعية: cell delivery, auto micro atomization device (AMAD), cell therapy, wound healing, organoids, Biotechnology, TP248.13-248.65

الوصف: Severe skin wounds are often associated with large areas of damaged tissue, resulting in substantial loss of fluids containing electrolytes and proteins. The net result is a vulnerability clinically to skin infections. Therapies aiming to close these large openings are effective in reducing the complications of severe skin wounds. Recently, cell transplantation therapy showed the potential for rapid re-epithelialization of severe skin wounds. Here, we show the improved effects of cell transplantation therapy using a robust protocol of efficient expansion and delivery of epidermal cells for treatment of severe skin wounds. Human skin tissues were used to generate human epidermal organoids maintained under newly established culture conditions. The human epidermal organoids showed an improved capacity of passaging for at least 10 rounds, enabling organoids to expand to cell numbers required for clinical applications. A newly designed auto micro-atomization device (AMAD) was developed for delivery of human epidermal organoids onto the sites of severe skin wounds enhancing uniform and concentrated delivery of organoids, facilitating their engraftment and differentiation for skin reconstitution. With the optimal design and using pneumatic AMAD, both survival and functions of organoids were effectively protected during the spraying process. Cells in the sprayed human epidermal organoids participated in the regeneration of the epidermis at wound sites in a mouse model and accelerated wound healing significantly. The novel AMAD and out new protocol with enhanced effects with respect to both organoid expansion and efficient transplantation will be used for clincal treatments of complex, uneven, or large-area severe skin wounds.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/article/10.3389/fbioe.2020.00110/fullTest; https://doaj.org/toc/2296-4185Test

الوصول الحر: https://doaj.org/article/b06e2fe46d4641d08340114a00b43c47Test

المؤلفون: Haiyun Pei, Chao Zhai, Huilin Li, Fang Yan, Jinhua Qin, Hongfeng Yuan, Rui Zhang, Shuyong Wang, Wencheng Zhang, Mingyang Chang, Yunfang Wang, Xuetao Pei

المصدر: Stem Cell Research & Therapy, Vol 8, Iss 1, Pp 1-11 (2017)

مصطلحات موضوعية: Connexin, p38 MAPK, Hepatic progenitor cell, Hepatocyte, Differentiation, Cell therapy, Medicine (General), R5-920, Biochemistry, QD415-436

الوصف: Abstract Background Bi-potential hepatic progenitor cells can give rise to both hepatocytes and cholangiocytes, which is the last phase and critical juncture in terms of sequentially hepatic lineage restriction from any kind of stem cells. If their differentiation can be controlled, it might access to functional hepatocytes to develop pharmaceutical and biotechnology industries as well as cell therapies for end-stage liver diseases. Methods In this study, we investigated the influence of Cx32 and Cx43 on hepatocyte differentiation of WB-F344 cells by in vitro gain and loss of function analyses. An inhibitor of Cx32 was also used to make further clarification. To reveal p38 MAPK pathway is closely related to Cxs, rats with 70% partial hepatectomy were injected intraperitoneally with a p38 inhibitor, SB203580. Besides, the effects of p38 MAPK pathway on differentiation of hepatoblasts isolated from fetal rat livers were evaluated by addition of SB203580 in culture medium. Results In vitro gain and loss of function analyses showed overexpression of Connexin 32 and knockdown of Connexin 43 promoted hepatocytes differentiation from hepatic progenitor cells. In addition, in vitro and ex vivo research revealed inhibition of p38 mitogen-activated protein kinase pathway can improve hepatocytes differentiation correlating with upregulation of Connexin 32 expression and downregulation of Connexin 43 expression. Conclusions Here we demonstrate that Connexins play crucial roles in facilitating differentiation of hepatic progenitors. Our work further implicates that regulators of Connexins and their related pathways might provide new insights to improve lineage restriction of stem cells to mature hepatocytes.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s13287-017-0703-2Test; https://doaj.org/toc/1757-6512Test

الوصول الحر: https://doaj.org/article/279cea40ddfc481c9ad530a192284d29Test

المؤلفون: Mingyang Chang, Xiangjin Ma, Jiaqi Han, Hao Xue, Haixia Liu, Long Li

المصدر: IEEE Transactions on Industrial Electronics. 70:10710-10719

مصطلحات موضوعية: Control and Systems Engineering, Electrical and Electronic Engineering

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::403415237fa5644a4696b32266272115Test
https://doi.org/10.1109/tie.2022.3220908Test