المؤلفون: Di Chen, Min Liao, Xiao-ru Wu, Tang-yuan-meng Zhao, Hu Sun

المصدر: Scientific Reports, Vol 14, Iss 1, Pp 1-10 (2024)

مصطلحات موضوعية: Remimazolam, Elderly patients, Gastroscopy, Propofol, Effective dose, Medicine, Science

الوصف: Abstract Remimazolam, a novel intravenous anesthetic, has been proven to be safe and efficacious in the gastroscopy setting among the elderly. However, reports comparing the effectiveness and safety of using equivalent doses of remimazolam with propofol have not been seen. The aim of this study was to compare the sedation efficacy and safety of the 95% effective doses (ED95) of remimazolam versus propofol combined with sufentanil in the gastroscopy setting among the elderly. In the first step of this two-step study, a modified up-and-down method was used to calculate the ED95 of remimazolam and propofol when combined with 0.1 µg/kg sufentanil in inhibiting body movement of elderly patients undergoing gastroscopy. In the second step, ED95 of both agents calculated in the first step were administered, endpoints of efficacy, safety, and incidence of adverse events were compared. A total of 46 individuals completed the first step. The ED95 of remimazolam was 0.163 mg/kg (95% CI 0.160–0.170 mg/kg), and that of propofol was 1.042 mg/kg (95% CI 1.007–1.112 mg/kg). In the second step, 240 patients completed the trial. The anesthetic effective rates of the remimazolam group and the propofol group were 78% and 83%, respectively, with no statistical difference (P = 0.312). Patients in the remimazolam group had more stable circulatory functions (P

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/8f0ce51e209743db8d1b201f2589cb40Test

المؤلفون: Changfan Ju, Binjian Zeng, Ziqi Luo, Zhibin Yang, Puqi Hao, Luocheng Liao, Qijun Yang, Qiangxiang Peng, Shuaizhi Zheng, Yichun Zhou, Min Liao

المصدر: Journal of Materiomics, Vol 10, Iss 2, Pp 277-284 (2024)

مصطلحات موضوعية: Al2O3, BEOL processing, Ferroelectricity, Hf0.5Zr0.5O2 (HZO), Materials of engineering and construction. Mechanics of materials, TA401-492

الوصف: Hf0.5Zr0.5O2 (HZO) ferroelectric thin films have gained significant attention for the development of next-generation ferroelectric memories by complementary-metal-oxide semiconductor (CMOS) back-end-of-line (BEOL) processing, due to their relatively low crystallization temperature. However, it remains challenging to achieve excellent ferroelectric properties with post deposition annealing (PDA) process at a BEOL compatible temperature. Along these lines, in this work, it is demonstrated that the ferroelectricity of 15 nm thick HZO thin film prepared by PDA process at 400 °C can be improved to varying degrees, via depositing 2 nm thick dielectric layers of Al2O3, HfO2, or ZrO2 at either the bottom or the top of the film. Notably, the HZO thin film with the top-Al2O3 layer exhibits remarkable ferroelectric properties, which are independent of the thickness of HZO. The 6 nm thick HZO thin film shows a total remanent polarization (2Pr) of 31 μC/cm2 under an operating voltage of 2.5 V. These results represent a significant advancement in the fabrication of high-performance, BEOL compatible ferroelectric memories, as compared to previously reported state-of-the-art works.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2352847823001107Test; https://doaj.org/toc/2352-8478Test

الوصول الحر: https://doaj.org/article/e1c66f6c46f24303af4852d15c97d7ebTest

المؤلفون: Ye Tian, Tianqi Yu, Jun Wang, Haoxiang Zhang, Yingna Jian, Xiuping Li, Geping Wang, Guanghua Wang, Yong Hu, Chenhe Lu, Jiyong Zhou, Liqing Ma, Min Liao

المصدر: Frontiers in Microbiology, Vol 15 (2024)

مصطلحات موضوعية: wildbirds, Qinghai Lake, Deltacoronavirus, black-headed gull, receptor-binding domain (RBD), Microbiology, QR1-502

الوصف: Deltacoronavirus, widely distributed among pigs and wild birds, pose a significant risk of cross-species transmission, including potential human epidemics. Metagenomic analysis of bird samples from Qinghai Lake, China in 2021 reported the presence of Deltacoronavirus. A specific gene fragment of Deltacoronavirus was detected in fecal samples from wild birds at a positive rate of 5.94% (6/101). Next-generation sequencing (NGS) identified a novel Deltacoronavirus strain, which was closely related to isolates from the United Arab Emirates (2018), China (2022), and Poland (2023). Subsequently the strain was named A/black-headed gull/Qinghai/2021(BHG-QH-2021) upon confirmation of the Cytochrome b gene of black-headed gull in the sample. All available genome sequences of avian Deltacoronavirus, including the newly identified BHG-QH-2021 and 5 representative strains of porcine Deltacoronavirus (PDCoV), were classified according to ICTV criteria. In contrast to Coronavirus HKU15, which infects both mammals and birds and shows the possibility of cross-species transmission from bird to mammal host, our analysis revealed that BHG-QH-2021 is classified as Putative species 4. Putative species 4 has been reported to infect 5 species of birds but not mammals, suggesting that cross-species transmission of Putative species 4 is more prevalent among birds. Recombination analysis traced BHG-QH-2021 origin to dut148cor1 and MW01_1o strains, with MW01_1o contributing the S gene. Surprisingly, SwissModle prediction showed that the optimal template for receptor-binding domain (RBD) of BHG-QH-2021 is derived from the human coronavirus 229E, a member of the Alphacoronavirus, rather than the anticipated RBD structure of PDCoV of Deltacoronavirus. Further molecular docking analysis revealed that substituting the loop 1–2 segments of HCoV-229E significantly enhanced the binding capability of BHG-QH-2021 with human Aminopeptidase N (hAPN), surpassing its native receptor-binding domain (RBD). Most importantly, this finding was further confirmed by co-immunoprecipitation experiment that loop 1–2 segments of HCoV-229E enable BHG-QH-2021 RBD binding to hAPN, indicating that the loop 1–2 segment of the RBD in Putative species 4 is a probable key determinant for the virus ability to spill over into humans. Our results summarize the phylogenetic relationships among known Deltacoronavirus, reveal an independent putative avian Deltacoronavirus species with inter-continental and inter-species transmission potential, and underscore the importance of continuous surveillance of wildlife Deltacoronavirus.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fmicb.2024.1423367/fullTest; https://doaj.org/toc/1664-302XTest

الوصول الحر: https://doaj.org/article/70167d877d9b47099b454012696356ceTest

المؤلفون: Zheng Wang, Min Liao, Li Ling, Meng Zhang

المصدر: Giant, Vol 18, Iss , Pp 100266- (2024)

مصطلحات موضوعية: Photocatalytic interface, Optical fiber, Photo-deposition, Noble metal sites, Hydroxyl radicals, Science (General), Q1-390

الوصف: Anchoring noble metal sites through photo-reduction is an effective way to modulate charge distribution on photocatalytic interfaces, thereby enhancing photocatalytic performance. The irradiation field on the exposed surfaces of photocatalysts has an important influence on the morphology and distribution of noble metal sites. However, non-uniform light field derived from the scattering effects of particle-form photocatalysts hinders the well-distributed photo-deposition of noble metal sites. To address this challenge, we proposed a photo-deposition method utilizing the optical fiber coated with photocatalysts. TiO2 nanorod (NR) array was coated on the optical fiber to achieve a well-distributed irradiation filed across the NR array. This resulted in a concentration of the irradiation field primarily within the NR array, maintaining uniformly distributed light intensities throughout. Palladium (Pd) sites dominated by nanoclusters were well distributed on TiO2 NR array utilizing a photo-reduction method. These Pd sites functioned as electron acceptors, facilitating the effective separation and transfer of photo-generated carriers. Consequently, an highly active photocatalytic reaction interface was constructed, demonstrating the accumulation of a substantial concentration of holes and their efficient conversion into hydroxyl radicals (·OH). Notably, hydroxyl radicals with a concentration of 62.6 μM could be generated within 14.4 min. The construction of this efficient photocatalytic interface offers an optimal platform for accelerating photocatalytic reactions and enhancing photocatalytic efficiency.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2666542524000316Test; https://doaj.org/toc/2666-5425Test

الوصول الحر: https://doaj.org/article/8b0d9d5cb5c84b1c910b099561af8c55Test

المؤلفون: Xiaoru Wu, Min Liao, Xingzhou Lin, Jianing Hu, Tangyuanmeng Zhao, Hu Sun

المصدر: BMC Anesthesiology, Vol 24, Iss 1, Pp 1-8 (2024)

مصطلحات موضوعية: Ciprofol, Alfentanil, Gastroscopy, Effective dose, Dixon’s up-and-down method, Anesthesiology, RD78.3-87.3

الوصف: Abstract Background Ciprofol is a novel intravenous sedative and anesthetic. Studies have shown that it features a rapid onset of action, a fast recovery time, slight inhibition of respiratory and cardiovascular functions, and a low incidence of adverse reactions. This study aims to explore the median effective dose (ED50) and the 95% effective dose (ED95) of ciprofol in inhibiting responses to gastroscope insertion when combined with a low dose of alfentanil, and to evaluate its safety, to provide a reference for the rational use of ciprofol in clinical practices. Methods We included 25 patients aged 18–64 years of either sex who underwent gastroscopy under intravenous general anesthesia, with a Body Mass Index (BMI) 18–28 kg/m2, and an American Society of Anesthesiologists (ASA) grade I or II. In this study, the dose-finding strategy of ciprofol followed a modified Dixon’s up-and-down method with an initial dose of 0.30 mg/kg and an increment of 0.02 mg/kg. Ciprofol was administered after intravenous injection of 7 µg/kg of alfentanil, and 2 min later a gastroscope was inserted. When the insertion response of one participant was positive (including body movement, coughing, and eye opening), an escalation of 0.02 mg/kg would be given to the next participant; otherwise, a de-escalation of 0.02 mg/kg would be administered. The study was terminated when negative response and positive response alternated 8 times. A Probit model was used to calculate the ED50 and ED95 of ciprofol in inhibiting responses to gastroscope insertion when combined with alfentanil. Patients’ recovery time, discharge time, vital signs and occurrence of adverse reactions were recorded. Results The ED50 of single-dose intravenous ciprofol injection with 7 µg/kg of alfentanil in inhibiting gastroscope insertion responses was 0.217 mg/kg, and the ED95 was 0.247 mg/kg. Patients’ recovery time and discharge time were 11.04 ± 1.49 min and 9.64 ± 2.38 min, respectively. The overall incidence of adverse reactions was 12%. Conclusion The ED50 of ciprofol combined with 7 µg/kg of alfentanil in inhibiting gastroscope insertion responses was 0.217 mg/kg, and the ED95 was 0.247 mg/kg. Ciprofol showed a low incidence of anesthesia-related adverse events. Trial registration http://www.chictr.org.cnTest (ChiCTR2200061727).

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2253Test

الوصول الحر: https://doaj.org/article/a360c251a1754699a797bd0a7046a32cTest

المؤلفون: Junyi Zhang, Min Liao, Bo Xu, Hai He

المصدر: Mitochondrial DNA. Part B. Resources, Vol 9, Iss 1, Pp 100-103 (2024)

مصطلحات موضوعية: Gastrochilus sinensis, chloroplast genome, phylogeny, Genetics, QH426-470

الوصف: AbstractGastrochilus sinensis is a beautiful epiphytic orchid with high ornamental value. In this study, the first complete chloroplast genome sequence of G. sinensis was determined using next-generation sequencing (NGS). The de novo assembled chloroplast genome was 148,020 bp in length, including a pair of inverted repeat regions (IRs; 25,987 bp), a small single-copy region (SSC; 11,045 bp), and a large single-copy region (LSC; 85,001 bp). The chloroplast genome encodes 109 unique genes, including 75 protein-coding genes (PCGs), 30 tRNA genes, and four rRNA genes. The total GC content of the chloroplast genome was 36.8%. The phylogenetic analysis showed a close relationship between G. sinensis and G. formosanus species. The complete chloroplast genome provides fundamental information for genetic diversity and phylogenetic relationships in Gastrochilus.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2380-2359Test

الوصول الحر: https://doaj.org/article/e8313bdd7352432981fdb5f8aa608d93Test

المؤلفون: Na Li, Yunlong Zhu, Fangqun Cheng, Yongliang Chen, Xin Peng, Mingxin Wu, Haobo Huang, Lingling Zhang, Min Liao, Sha Xiao, Hui Zhang, Yuying Zhou, Sihao Chen, Zhican Liu, Liqing Yi, Yiqun Peng, Jie Fan, Jianping Zeng

المصدر: ESC Heart Failure, Vol 10, Iss 5, Pp 2882-2894 (2023)

مصطلحات موضوعية: Atrial fibrillation, Heart failure, HFmrEF, Impact, Outcome, Diseases of the circulatory (Cardiovascular) system, RC666-701

الوصف: Abstract Aims Atrial fibrillation (AF) and heart failure (HF) often co‐exist and are closely intertwined. The impact of AF on the outcome of patients with heart failure with mildly‐reduced ejection fraction (HFmrEF) is not fully clear. This study aimed to investigate the impact of AF on the outcomes of hospitalized HFmrEF patients. Methods and results The study included 1691 consecutive patients with HFmrEF (mean 68.2 years, 64.8% male) including 296 AF patients. Patients completed 1 year and mean of 33 month clinical follow‐up after discharge by telephone interview, clinical visit, or community visit. The primary endpoint was cerebro‐cardiovascular events (CCE, composite of HF rehospitalization, stroke, or cardiovascular death). After propensity score matching, 296 patients were included into the AF group (mean 71.5 years) and 592 patients into the non‐AF group (mean 70.6 years). After propensity score matching, CCE at 1 year (59.1% vs. 48.5%, P = 0.003) and at a mean of 33 month (77.0% vs. 70.6%, P = 0.043). AF was independently associated with increased CCE within 1 year (HR = 1.31, 95% CI 1.07 to 1.61, P = 0.010) and at 33 months (HR = 1.20, 95% CI 1.00 to 1.43, P = 0.050) post‐discharge after adjusted for other clinical confounders including discharge heart rate, NT‐proBNP, haemoglobin, and uric acid. Conclusions AF is independently associated with an increased risk of CCE in HFmrEF patients within 1 year and at a mean of 33 months after discharge.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2055-5822Test

الوصول الحر: https://doaj.org/article/4606327584fb41758e8afc6950074104Test

المؤلفون: Jinpeng Du, Wenwei Liao, Haichuan Wang, Guimin Hou, Min Liao, Lin Xu, Jiwei Huang, Kefei Yuan, Xiangzheng Chen, Yong Zeng

المصدر: Signal Transduction and Targeted Therapy, Vol 8, Iss 1, Pp 1-15 (2023)

مصطلحات موضوعية: Medicine, Biology (General), QH301-705.5

الوصف: Abstract The mineral dust-induced gene (MDIG) comprises a conserved JmjC domain and has the ability to demethylate histone H3 lysine 9 trimethylation (H3K9me3). Previous studies have indicated the significance of MDIG in promoting cell proliferation by modulating cell-cycle transition. However, its involvement in liver regeneration has not been extensively investigated. In this study, we generated mice with liver-specific knockout of MDIG and applied partial hepatectomy or carbon tetrachloride mouse models to investigate the biological contribution of MDIG in liver regeneration. The MDIG levels showed initial upregulation followed by downregulation as the recovery progressed. Genetic MDIG deficiency resulted in dramatically impaired liver regeneration and delayed cell cycle progression. However, the MDIG-deleted liver was eventually restored over a long latency. RNA-seq analysis revealed Myc as a crucial effector downstream of MDIG. However, ATAC-seq identified the reduced chromatin accessibility of OTX2 locus in MDIG-ablated regenerating liver, with unaltered chromatin accessibility of Myc locus. Mechanistically, MDIG altered chromatin accessibility to allow transcription by demethylating H3K9me3 at the OTX2 promoter region. As a consequence, the transcription factor OTX2 binding at the Myc promoter region was decreased in MDIG-deficient hepatocytes, which in turn repressed Myc expression. Reciprocally, Myc enhanced MDIG expression by regulating MDIG promoter activity, forming a positive feedback loop to sustain hepatocyte proliferation. Altogether, our results prove the essential role of MDIG in facilitating liver regeneration via regulating histone methylation to alter chromatin accessibility and provide valuable insights into the epi-transcriptomic regulation during liver regeneration.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2059-3635Test

الوصول الحر: https://doaj.org/article/23e9ee1dc2774635ae5a75999ca3fb9dTest

المؤلفون: Jie Yang, Jiayan Huang, Yonggang Zhang, Keyu Zeng, Min Liao, Zhenpeng Jiang, Wuyongga Bao, Qiang Lu, Lishao Guo

المصدر: Chinese Medical Journal, Vol 136, Iss 17, Pp 2028-2036 (2023)

مصطلحات موضوعية: Medicine

الوصف: Abstract. Background:. Patients with mass-forming pancreatitis (MFP) or pancreatic ductal adenocarcinoma (PDAC) presented similar clinical symptoms, but required different treatment approaches and had different survival outcomes. This meta-analysis aimed to compare the diagnostic performance of contrast-enhanced ultrasound (CEUS) and contrast-enhanced computed tomography (CECT) in differentiating MFP from PDAC. Methods:. A literature search was performed in the PubMed, EMBASE (Ovid), Cochrane Library (CENTRAL), China National Knowledge Infrastructure (CNKI), Weipu (VIP), and WanFang databases to identify original studies published from inception to August 20, 2021. Studies reporting the diagnostic performances of CEUS and CECT for differentiating MFP from PDAC were included. The meta-analysis was performed with Stata 15.0 software. The outcomes included the pooled sensitivity, specificity, positive likelihood ratio (+LR), negative likelihood ratio (−LR), diagnostic odds ratio (DOR), and summary receiver operating characteristic (SROC) curves of CEUS and CECT. Meta-regression was conducted to investigate heterogeneity. Bayesian network meta-analysis was conducted to indirectly compare the overall diagnostic performance. Results:. Twenty-six studies with 2115 pancreatic masses were included. The pooled sensitivity and specificity of CEUS for MFP were 82% (95% confidence interval [CI], 73%–88%; I2 = 0.00%) and 95% (95% CI, 90%–97%; I2 = 63.44%), respectively; the overall +LR, −LR, and DOR values were 15.12 (95% CI, 7.61–30.01), 0.19 (95% CI, 0.13–0.29), and 78.91 (95% CI, 30.94–201.27), respectively; and the area under the SROC curve (AUC) was 0.90 (95% CI, 0.87–92). However, the overall sensitivity and specificity of CECT were 81% (95% CI, 75–85%; I2 = 66.37%) and 94% (95% CI, 90–96%; I2 = 74.87%); the overall +LR, −LR, and DOR values were 12.91 (95% CI, 7.86–21.20), 0.21 (95% CI, 0.16–0.27), and 62.53 (95% CI, 34.45–113.51), respectively; and, the SROC AUC was 0.92 (95% CI, 0.90–0.94). The overall diagnostic accuracy of CEUS was comparable to that of CECT for the differential diagnosis of MFP and PDAC (relative DOR 1.26, 95% CI [0.42–3.83], P > 0.05). Conclusions:. CEUS and CECT have comparable diagnostic performance for differentiating MFP from PDAC, and should be considered as mutually complementary diagnostic tools for suspected focal pancreatic lesions.

وصف الملف: electronic resource

العلاقة: http://journals.lww.com/10.1097/CM9.0000000000002300Test; https://doaj.org/toc/0366-6999Test; https://doaj.org/toc/2542-5641Test

الوصول الحر: https://doaj.org/article/29c03474668f422588cb589b0ec47735Test

المؤلفون: Jun-Yi Zhang, Yue-Hong Cheng, Min Liao, Gui-Ying Liu, Pan-Yan Yang, Bo Xu, Hai He

المصدر: PhytoKeys, Vol 230, Iss , Pp 145-156 (2023)

مصطلحات موضوعية: Botany, QK1-989

الوصف: Thrixspermum taeniophyllum is described as a new orchid species from Wenchuan County, Sichuan Province of southwest China. It is morphologically similar to T. japonicum, but it differs from the latter in having branched stems, slightly fleshy strap-shaped leaves, longer inflorescences with 3–6 flowers and a capitate gynandrium with a lip-shaped mouth opening. Its species status is also supported by molecular phylogenetic analyses, based on nuclear ribosome internal transcribed spacer (nrITS) and three chloroplast DNA fragments (matK, psbA-trnH and trnL-F), which showed distinct systematic boundaries from the most morphologically similar T. japonicum and their morphological relatives T. saruwatarii and T. pygmaeum.

وصف الملف: electronic resource

العلاقة: https://phytokeys.pensoft.net/article/104273/download/pdfTest/; https://phytokeys.pensoft.net/article/104273/download/xmlTest/; https://phytokeys.pensoft.net/article/104273Test/; https://doaj.org/toc/1314-2003Test

الوصول الحر: https://doaj.org/article/ad5ab4b0aaf945919eda7b59fcfddfedTest