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1دورية أكاديمية
المؤلفون: Hanssen, Hege Marie, Fjellstad, Maria Serafia, Skjevling, Linn, Johnsen, Peter Holger, Goll, Rasmus, Almå, Kristin Helen, Kulseng, Bård Eirik, Valle, Per Christian
مصطلحات موضوعية: VDP::Medisinske fag: 700::Klinisk medisinske fag: 750::Gasteroenterologi: 773, VDP::Midical sciences: 700::Clinical medical sciences: 750::Gastroenterology: 773, Mikrobiologi / Microbiology, Overvekt / Overweight
الوصف: Introduction - Obesity is one of the main threats to public health in western countries and increases the risk of several diseases, overall morbidity and mortality. Sustained weight loss will reduce risk factors and improve several obesity comorbidities. Options are conservative treatment such as lifestyle changes, bariatric surgery or medications. Conservative treatment has a low success rate, and bariatric surgery is typically not reversible, with the risk of complications and recurrences. Treatment of obesity with medications has in recent years shown great promise, but the side effects are many, and the long-term effect is unknown. There is also a need for an option for patients where surgery has contraindications and conservative follow-up does not succeed. The research on obesity and gut microbiota has yielded promising results regarding weight reduction and metabolic health, but more research is needed to better understand the relationship between gut microbiota and severe obesity. This study could show proof of concept that gut microbiota from a lean donor could, in addition to lifestyle intervention, contribute to weight reduction in people suffering from severe obesity. Method and analysis - This study aims to investigate if a fecal microbiota transplantation (FMT) from a lean donor leads to weight reduction in participants suffering from severe obesity. The study is a single-centre, double-blinded, placebo-controlled, parallel-group study with 60 participants. Participants will be randomised 1:1 for FMT from a lean donor or placebo. FMT or placebo will be delivered once by enema. We will include participants from the outpatient clinic for severe obesity, at the Medical Department, University Hospital of North Norway, Harstad, by invitation only. The study has a follow-up period of 12 months, with study visits of 3, 6 and 12 months post FMT. The primary endpoint is a weight reduction of ≥10%, 12 months after intervention. The results of the study will be published in open access journals. At the end of ...
العلاقة: BMJ Open; Helse Nord RHF: HNF1411-18; Hanssen, Fjellstad, Skjevling, Johnsen, Goll, Almå, Kulseng, Valle. Randomised, placebo-controlled, double-blinded trial of fecal microbiota transplantation in severe obesity: a study protocol. BMJ Open. 2023; FRIDAID 2220055; https://hdl.handle.net/10037/32488Test
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2دورية أكاديمية
المؤلفون: Dey, Hymonti, Simonovic, Danijela, Hagen, Ingrid Sofie Norberg-Schulz, Vasskog, Terje, Fredheim, Elizabeth G. Aarag, Blencke, Hans-Matti, Anderssen, Trude, Strøm, Morten B., Haug, Tor
مصطلحات موضوعية: VDP::Matematikk og naturvitenskap: 400::Kjemi: 440::Legemiddelkjemi: 448, VDP::Mathematics and natural scienses: 400::Chemistry: 440::Pharmaceutical chemistry: 448, Mikrobiologi / Microbiology
الوصف: We have synthesised short analogues of the marine antimicrobial peptide Turgencin A from the colonial Arctic ascidian Synoicum turgens. In this study, we focused on a central, cationic 12-residue Cys-Cys loop region within the sequence. Modified (tryptophan- and arginine-enriched) linear peptides were compared with Cys-Cys cyclic derivatives, and both linear and Cys-cyclic peptides were N-terminally acylated with octanoic acid (C 8 ), decanoic acid (C 10 ) or dodecanoic acid (C 12 ). The highest antimicrobial potency was achieved by introducing dodecanoic acid to a cyclic Turgencin A analogue with low intrinsic hydrophobicity, and by introducing octanoic acid to a cyclic analogue displaying a higher intrinsic hydrophobicity. Among all tested synthetic Turgencin A lipopeptide analogues, the most promising candidates regarding both antimicrobial and haemolytic activity were C 12 -cTurg-1 and C 8 -cTurg-2. These optimized cyclic lipopeptides displayed minimum inhibitory concentrations of 4 µg/mL against Staphylococcus aureus, Escherichia coli and the fungus Rhodothorula sp. Mode of action studies on bacteria showed a rapid membrane disruption and bactericidal effect of the cyclic lipopeptides. Haemolytic activity against human erythrocytes was low, indicating favorable selective targeting of bacterial cells.
العلاقة: Simonovic, D. (2023). Synthesis and structure-activity relationship studies of marine-derived antimicrobial peptides and small cyclic peptidomimetics. (Doctoral thesis). https://hdl.handle.net/10037/29375Test .; International Journal of Molecular Sciences; FRIDAID 2072594; https://hdl.handle.net/10037/27420Test
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3دورية أكاديمية
المؤلفون: Schneider, Yannik Karl-Heinz, Jenssen, Marte, Isaksson, Johan, Østnes Hansen, Kine, Andersen, Jeanette Hammer, Hansen, Espen
مصطلحات موضوعية: Antimicrobial / Antimicrobial, Bioaktive naturstoffer / Bioactive natural products, Biokjemi / Biochemistry, Cellegifter / cytostatika / Antineoplastic Agents, Mikrobiologi / Microbiology
الوصف: Siderophores are compounds with high affinity for ferric iron. Bacteria produce these compounds to acquire iron in iron-limiting conditions. Iron is one of the most abundant metals on earth, and its presence is necessary for many vital life processes. Bacteria from the genus Serratia contribute to the iron respiration in their environments, and previously several siderophores have been isolated from this genus. As part of our ongoing search for medicinally relevant compounds produced by marine microbes, a co-culture of a Shewanella sp. isolate and a Serratia sp. isolate, grown in iron-limited conditions, was investigated, and the rare siderophore serratiochelin A ( 1 ) was isolated with high yields. Compound 1 has previously been isolated exclusively from Serratia sp., and to our knowledge, there is no bioactivity data available for this siderophore to date. During the isolation process, we observed the degradation product serratiochelin C ( 2 ) after exposure to formic acid. Both 1 and 2 were verified by 1-D and 2-D NMR and high-resolution MS/MS. Here, we present the isolation of 1 from an iron-depleted co-culture of Shewanella sp. and Serratia sp., its proposed mechanism of degradation into 2 , and the chemical and biological characterization of both compounds. The effects of 1 and 2 on eukaryotic and prokaryotic cells were evaluated, as well as their effect on biofilm formation by Staphylococcus epidermidis. While 2 did not show bioactivity in the given assays, 1 inhibited the growth of the eukaryotic cells and Staphylococcus aureus .
العلاقة: Schneider, Y.K.-H. (2020). Bioactive secondary metabolites from bacteria. Natural products from marine and terrestrial bacteria, dereplication, isolation and investigation of bacterial secondary metabolites. (Doctoral thesis). https://hdl.handle.net/10037/19264Test; Jenssen, M. (2022). Bioprospecting of marine microorganisms for the discovery of antibacterial compounds - Isolation, structure elucidation and bioactivity assessment of marine microbial natural products. (Doctoral thesis). https://hdl.handle.net/10037/24722Test .; Microorganisms; info:eu-repo/grantAgreement/EC/H2020/721421/EU/Improving the flow in the pipeline of the next generation of marine biodiscovery scientists/MarPipe/; info:eu-repo/grantAgreement/RCN/BIOTEK2021/ 269425/Norway/DL: Digital discovery of antimicrobial molecules from marine Arctic resources with reduced risk of triggering resistance/DigiBiotics/; Schneider, Y., Jenssen, M., Isaksson, J., Hansen, K.Ø., Andersen, J.H. & Hansen, E.H. (2020). Bioactivity of Serratiochelin A, a Siderophore Isolated from a Co-Culture of Serratia sp. and Shewanella sp. Microorganisms, 8 (7), 1042.; FRIDAID 1820158; https://hdl.handle.net/10037/19078Test
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المؤلفون: Hymonti Dey, Danijela Simonovic, Ingrid Norberg-Schulz Hagen, Terje Vasskog, Elizabeth G. Aarag Fredheim, Hans-Matti Blencke, Trude Anderssen, Morten B. Strøm, Tor Haug
المصدر: International Journal of Molecular Sciences; Volume 23; Issue 22; Pages: 13844
مصطلحات موضوعية: VDP::Mathematics and natural scienses: 400::Chemistry: 440::Pharmaceutical chemistry: 448, Organic Chemistry, General Medicine, Peptides, Cyclic, Hemolysis, Catalysis, Computer Science Applications, Anti-Bacterial Agents, Inorganic Chemistry, Lipopeptides, AMPs, Cys-Cys cyclic peptides, lipopeptides, short antibacterial peptides, structure–activity relationship, mechanism of action, Anti-Infective Agents, Mikrobiologi / Microbiology, Cyclization, VDP::Matematikk og naturvitenskap: 400::Kjemi: 440::Legemiddelkjemi: 448, Escherichia coli, Humans, Physical and Theoretical Chemistry, Molecular Biology, Spectroscopy, Antimicrobial Peptides
الوصف: We have synthesised short analogues of the marine antimicrobial peptide Turgencin A from the colonial Arctic ascidian Synoicum turgens. In this study, we focused on a central, cationic 12-residue Cys-Cys loop region within the sequence. Modified (tryptophan- and arginine-enriched) linear peptides were compared with Cys-Cys cyclic derivatives, and both linear and Cys-cyclic peptides were N-terminally acylated with octanoic acid (C8), decanoic acid (C10) or dodecanoic acid (C12). The highest antimicrobial potency was achieved by introducing dodecanoic acid to a cyclic Turgencin A analogue with low intrinsic hydrophobicity, and by introducing octanoic acid to a cyclic analogue displaying a higher intrinsic hydrophobicity. Among all tested synthetic Turgencin A lipopeptide analogues, the most promising candidates regarding both antimicrobial and haemolytic activity were C12-cTurg-1 and C8-cTurg-2. These optimized cyclic lipopeptides displayed minimum inhibitory concentrations of 4 µg/mL against Staphylococcus aureus, Escherichia coli and the fungus Rhodothorula sp. Mode of action studies on bacteria showed a rapid membrane disruption and bactericidal effect of the cyclic lipopeptides. Haemolytic activity against human erythrocytes was low, indicating favorable selective targeting of bacterial cells.
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2e0f86fb7635e0ad6aaccbb6c576b18Test
https://pubmed.ncbi.nlm.nih.gov/36430320Test -
5دورية أكاديمية
المؤلفون: Dey, Hymonti, Simonovic, Danijela, Hagen, Ingrid Sofie Norberg-Schulz, Vasskog, Terje, Fredheim, Elizabeth G. Aarag, Blencke, Hans-Matti, Anderssen, Trude, Strøm, Morten B., Haug, Tor
المصدر: Dey H, Simonovic D, Hagen IN, Vasskog TV, Fredheim E, Blencke H, Anderssen T, Strøm mbs, Haug T. Synthesis and Antimicrobial Activity of Short Analogues of the Marine Antimicrobial Peptide Turgencin A: Effects of SAROptimizations, Cys-Cys Cyclization and Lipopeptide Modifications. International Jo
العلاقة: International Journal of Molecular Sciences
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6تقرير
مصطلحات موضوعية: Aquaculture, Marine fry, Production parameters, Microbiology, VDP::Matematikk og naturvitenskap: 400, VDP::Mathematics and natural scienses: 400, Akvakultur / Aquaculture, Marin yngel / Marine fry, Mikrobiologi / Microbiology, Produksjonsparameter / Production parameters
الوصف: Prosjektet «Overvåkingsprogram og kompetansehevingstiltak knyttet til vannkvalitet og fiskehelse i marine yngel- og settefiskanlegg (MarinVest)».har blitt utført i samarbeid med industrideltagerne Sterling White Halibut (SWH) med anleggene på Rørvik (Rørv) og Imsland (Imsl), Nordic Halibut (NH) med anleggene Norsk Kveite (NK) og Halibut (Hal), Atlantic Cod Farms (ACF) med anlegget Atlantic Cod Juveniles (ACJ), Marine Harvest Labrus (MHL), Fjord Gadus (FG), Sande Seafarm Productions (SSP) og Skretting, samt FoU-partnerne Norsk institutt for vannforskning og Universitetet i Bergen, Institutt for biologi ved avdeling for marin mikrobiologi. Vestlandsprogrammet for nye oppdrettsarter, Vestlandsrådet har finansiert prosjektet sammen med egeninnsats fra de deltagende bedriftene og økonomisk støtte fra NIVA. Hovedmålet for prosjektet har vært å øke forutsigbarheten og optimalisere driften av marine yngel- og settefiskanlegg gjennom å gi oppdretterne verktøy til å etablere et godt vannmiljø. Prosjektarbeidet har vært delt inn i fire deler: 1) Et overvåkingsprogram av inntaksvann, etter vannbehandling og i de ulike stadiene av produksjonen (klekkeri, plommesekkstadiet, startfòring (levendefôr), startfòring (tørrfôr) og settefisk). 2) Kurs i prøvetaking og vannanalyse. 3) Individuelle vurderinger av anleggenes drift basert på overvåkingsprogrammet og anonymiserte resultat. 4) Generelle retningslinjer for sikker drift med bakgrunn i overnevnte, utformet som en håndbok i marin yngelproduksjon. ; Fluctuating and unstable production of fry is a well-known problem for many marine fry producers. This limits the development of new marine species and strikes individual farmers hard. Suboptimal operation, fish diseases and poor growth can have serious consequences both in the juvenile stage and in the subsequent grow-out phase. There is generally little scientific literature on production of marine fry although some species has been farmed for a long time. It is important to obtain this kind of experience and knowledge through ...
وصف الملف: application/pdf
العلاقة: NIVA-rapport;6782; urn:isbn:978-82-577-6517-0; urn:issn:1894-7948; http://hdl.handle.net/1956/10630Test; cristin:1250807
الإتاحة: http://hdl.handle.net/1956/10630Test
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المؤلفون: Yannik Karl-Heinz Schneider, Kine Østnes Hansen, Marte Jenssen, Johan Isaksson, Espen Hansen, Jeanette Hammer Andersen
المصدر: Microorganisms, Vol 8, Iss 1042, p 1042 (2020)
Microorganisms
Volume 8
Issue 7مصطلحات موضوعية: Microbiology (medical), Siderophore, Serratiochelin A, siderophore, natural products, Formic acid, 010501 environmental sciences, Bioaktive naturstoffer / Bioactive natural products, 010402 general chemistry, medicine.disease_cause, Serratiochelin C, anticancer, 01 natural sciences, Microbiology, Serratia, Serratia sp, chemistry.chemical_compound, iron, Biokjemi / Biochemistry, Mikrobiologi / Microbiology, Staphylococcus epidermidis, Virology, medicine, lcsh:QH301-705.5, microbial biotechnology, degradation, 0105 earth and related environmental sciences, biology, Biofilm, S. aureus, biology.organism_classification, Isolation (microbiology), 0104 chemical sciences, antibacterial, chemistry, lcsh:Biology (General), Staphylococcus aureus, Cellegifter / cytostatika / Antineoplastic Agents, Antimicrobial / Antimicrobial, Bacteria
الوصف: Siderophores are compounds with high affinity for ferric iron. Bacteria produce these compounds to acquire iron in iron-limiting conditions. Iron is one of the most abundant metals on earth, and its presence is necessary for many vital life processes. Bacteria from the genus Serratia contribute to the iron respiration in their environments, and previously several siderophores have been isolated from this genus. As part of our ongoing search for medicinally relevant compounds produced by marine microbes, a co-culture of a Shewanella sp. isolate and a Serratia sp. isolate, grown in iron-limited conditions, was investigated, and the rare siderophore serratiochelin A (1) was isolated with high yields. Compound 1 has previously been isolated exclusively from Serratia sp., and to our knowledge, there is no bioactivity data available for this siderophore to date. During the isolation process, we observed the degradation product serratiochelin C (2) after exposure to formic acid. Both 1 and 2 were verified by 1-D and 2-D NMR and high-resolution MS/MS. Here, we present the isolation of 1 from an iron-depleted co-culture of Shewanella sp. and Serratia sp., its proposed mechanism of degradation into 2, and the chemical and biological characterization of both compounds. The effects of 1 and 2 on eukaryotic and prokaryotic cells were evaluated, as well as their effect on biofilm formation by Staphylococcus epidermidis. While 2 did not show bioactivity in the given assays, 1 inhibited the growth of the eukaryotic cells and Staphylococcus aureus.
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::445f113221252f711565d09a5a4dd27eTest
https://www.mdpi.com/2076-2607/8/7/1042Test -
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مصطلحات موضوعية: Akvakultur / Aquaculture, Marin yngel / Marine fry, Marine fry, Produksjonsparameter / Production parameters, Matematikk og naturvitenskap: 400 [VDP], Mathematics and natural scienses: 400 [VDP], Mikrobiologi / Microbiology, Aquaculture, Production parameters, Microbiology
الوصف: Prosjektet «Overvåkingsprogram og kompetansehevingstiltak knyttet til vannkvalitet og fiskehelse i marine yngel- og settefiskanlegg (MarinVest)».har blitt utført i samarbeid med industrideltagerne Sterling White Halibut (SWH) med anleggene på Rørvik (Rørv) og Imsland (Imsl), Nordic Halibut (NH) med anleggene Norsk Kveite (NK) og Halibut (Hal), Atlantic Cod Farms (ACF) med anlegget Atlantic Cod Juveniles (ACJ), Marine Harvest Labrus (MHL), Fjord Gadus (FG), Sande Seafarm Productions (SSP) og Skretting, samt FoU-partnerne Norsk institutt for vannforskning og Universitetet i Bergen, Institutt for biologi ved avdeling for marin mikrobiologi. Vestlandsprogrammet for nye oppdrettsarter, Vestlandsrådet har finansiert prosjektet sammen med egeninnsats fra de deltagende bedriftene og økonomisk støtte fra NIVA. Hovedmålet for prosjektet har vært å øke forutsigbarheten og optimalisere driften av marine yngel- og settefiskanlegg gjennom å gi oppdretterne verktøy til å etablere et godt vannmiljø. Prosjektarbeidet har vært delt inn i fire deler: 1) Et overvåkingsprogram av inntaksvann, etter vannbehandling og i de ulike stadiene av produksjonen (klekkeri, plommesekkstadiet, startfòring (levendefôr), startfòring (tørrfôr) og settefisk). 2) Kurs i prøvetaking og vannanalyse. 3) Individuelle vurderinger av anleggenes drift basert på overvåkingsprogrammet og anonymiserte resultat. 4) Generelle retningslinjer for sikker drift med bakgrunn i overnevnte, utformet som en håndbok i marin yngelproduksjon. Fluctuating and unstable production of fry is a well-known problem for many marine fry producers. This limits the development of new marine species and strikes individual farmers hard. Suboptimal operation, fish diseases and poor growth can have serious consequences both in the juvenile stage and in the subsequent grow-out phase. There is generally little scientific literature on production of marine fry although some species has been farmed for a long time. It is important to obtain this kind of experience and knowledge through systematic mapping and sound scientific research. Such documentation and experience must be transferred to the farmers in order to strengthen the development and commercialization of marine species. In this way it is possible to improve decision-making and raise the competence of the individual farmer. Adverse water quality is mentioned frequently as a limiting factor and a source of suboptimal operation. publishedVersion
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=od________24::ddcdfe9efa069625301e5c0b775b2fc1Test
https://hdl.handle.net/1956/10630Test -
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المساهمون: Hess-Erga, Ole-Kristian - Project manager
مصطلحات موضوعية: Akvakultur / Aquaculture, Marin yngel / Marine fry, Marine fry, Produksjonsparameter / Production parameters, Matematikk og naturvitenskap: 400 [VDP], Mathematics and natural scienses: 400 [VDP], Mikrobiologi / Microbiology, Aquaculture, Production parameters, Microbiology
الوصف: Prosjektet «Overvåkingsprogram og kompetansehevingstiltak knyttet til vannkvalitet og fiskehelse i marine yngel- og settefiskanlegg (MarinVest)».har blitt utført i samarbeid med industrideltagerne Sterling White Halibut (SWH) med anleggene på Rørvik (Rørv) og Imsland (Imsl), Nordic Halibut (NH) med anleggene Norsk Kveite (NK) og Halibut (Hal), Atlantic Cod Farms (ACF) med anlegget Atlantic Cod Juveniles (ACJ), Marine Harvest Labrus (MHL), Fjord Gadus (FG), Sande Seafarm Productions (SSP) og Skretting, samt FoU-partnerne Norsk institutt for vannforskning og Universitetet i Bergen, Institutt for biologi ved avdeling for marin mikrobiologi. Vestlandsprogrammet for nye oppdrettsarter, Vestlandsrådet har finansiert prosjektet sammen med egeninnsats fra de deltagende bedriftene og økonomisk støtte fra NIVA. Hovedmålet for prosjektet har vært å øke forutsigbarheten og optimalisere driften av marine yngel- og settefiskanlegg gjennom å gi oppdretterne verktøy til å etablere et godt vannmiljø. Prosjektarbeidet har vært delt inn i fire deler: 1) Et overvåkingsprogram av inntaksvann, etter vannbehandling og i de ulike stadiene av produksjonen (klekkeri, plommesekkstadiet, startfòring (levendefôr), startfòring (tørrfôr) og settefisk). 2) Kurs i prøvetaking og vannanalyse. 3) Individuelle vurderinger av anleggenes drift basert på overvåkingsprogrammet og anonymiserte resultat. 4) Generelle retningslinjer for sikker drift med bakgrunn i overnevnte, utformet som en håndbok i marin yngelproduksjon. Fluctuating and unstable production of fry is a well-known problem for many marine fry producers. This limits the development of new marine species and strikes individual farmers hard. Suboptimal operation, fish diseases and poor growth can have serious consequences both in the juvenile stage and in the subsequent grow-out phase. There is generally little scientific literature on production of marine fry although some species has been farmed for a long time. It is important to obtain this kind of experience and knowledge through systematic mapping and sound scientific research. Such documentation and experience must be transferred to the farmers in order to strengthen the development and commercialization of marine species. In this way it is possible to improve decision-making and raise the competence of the individual farmer. Adverse water quality is mentioned frequently as a limiting factor and a source of suboptimal operation. Vestlandsrådet, Vestlandsprogrammet for nye oppdrettsarter
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::ad418b823349c28b134d4c99ee6f66e6Test
http://hdl.handle.net/11250/285802Test -
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النص الكامل