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1دورية أكاديمية
المؤلفون: Xiaoli Zhang, Wei Chen, Ping Li, Raul Calvo, Noel Southall, Xin Hu, Melanie Bryant-Genevier, Xinghua Feng, Qi Geng, Chenlang Gao, Meimei Yang, Kaiyuan Tang, Marc Ferrer, Juan Jose Marugan, Haoxing Xu
المصدر: eLife, Vol 8 (2019)
مصطلحات موضوعية: lysosomal two-pore channels, tri-cyclic antidepressants, whole-endolysosome patch clamp, lysosomal Na+ channels, Medicine, Science, Biology (General), QH301-705.5
الوصف: Mammalian two-pore-channels (TPC1, 2; TPCN1, TPCN2) are ubiquitously- expressed, PI(3,5)P2-activated, Na+-selective channels in the endosomes and lysosomes that regulate luminal pH homeostasis, membrane trafficking, and Ebola viral infection. Whereas the channel activity of TPC1 is strongly dependent on membrane voltage, TPC2 lacks such voltage dependence despite the presence of the presumed ‘S4 voltage-sensing’ domains. By performing high-throughput screening followed by lysosomal electrophysiology, here we identified a class of tricyclic anti-depressants (TCAs) as small-molecule agonists of TPC channels. TCAs activate both TPC1 and TPC2 in a voltage-dependent manner, referred to as Lysosomal Na+ channel Voltage-dependent Activators (LyNa-VAs). We also identified another compound which, like PI(3,5)P2, activates TPC2 independent of voltage, suggesting the existence of agonist-specific gating mechanisms. Our identification of small-molecule TPC agonists should facilitate the studies of the cell biological roles of TPCs and can also readily explain the reported effects of TCAs in the modulation of autophagy and lysosomal functions.
وصف الملف: electronic resource
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المؤلفون: Vaughan Higgins, Daniël van der Velden, Noemie Bechtet, Melanie Bryant, Jane Battersby, Melissa Belle, Laurens Klerkx
المساهمون: University of Tasmania [Hobart, Australia] (UTAS), Research Institute for Agricultural, Fisheries and Food (ILVO), AGroécologie, Innovations, teRritoires (AGIR), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Auteur indépendant, Wageningen University and Research [Wageningen] (WUR), AgriFutures Australia PRJ-009181, European Project: 727577,H2020-RUR-2016-2,AgriLink(2017), European Project: 818194,DESIRA(2019)
المصدر: Journal of Rural Studies
Journal of Rural Studies, 2023, 100, pp.103023. ⟨10.1016/j.jrurstud.2023.103023⟩
Journal of Rural Studies 100 (2023)
Journal of Rural Studies, 100مصطلحات موضوعية: Assemblage thinking, Sociology and Political Science, Precision agriculture, [SDV]Life Sciences [q-bio], Geography, Planning and Development, WASS, Development, Tinkering, Agency, Technologie and Innovatie, Digital agriculture, Knowledge Technology and Innovation, Kennis, Kennis, Technologie and Innovatie, Support networks, Agriculture 4.0
الوصف: Assemblage thinking is an increasingly influential approach in critical studies of food and farming, and partic-ularly in research on new agri-food technology such as precision agriculture (PA). This research is important in highlighting the distributed forms of power and agency through which farming worlds are assembled, and what these engender for more sustainable and equitable farming futures. However, to date, there has been limited attention to assembling PA from the perspective of farmers, what Legun and Burch (2021) refer to as 'deliberative assembling'. This paper contributes to knowledge in this area by applying post actor network theoretical work to investigate across case studies in Australia, the Netherlands and France, the forms of tinkering by which farmers attempt to make PA workable, and what these engender for farmer agency. Through our analysis, we show that much of the tinkering by farmers is aimed at holding together their own priorities, routines, and experiences with practices inscribed in PA technology, such as dependence on commercial advice, data-driven knowledge, and commitment to a single technological platform/company. Integral to this tinkering work are support networks that include agronomists, advisors, machinery dealers and/or farmer discussion groups. We argue that whilst these support networks are critical to holding together different practices, and making PA workable, they also play a more diverse and nuanced role in PA implementation than what has previously been recognised. Our case studies provide insights into three key forms of tinkering used by farmers in navigating support networks to make PA workable - disconnection, experimentation and trial-and-error, and trade-offs and compromises - and the specific distributions of agency which these tinkering practices engender. In conclusion, we argue that a tinkering lens provides a valuable approach for enabling agri-food scholars to tease out in greater depth delib-erative assembling practices and how these variously open-up or foreclose options for farmers in making PA workable.
وصف الملف: text/html; application/octet-stream
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74ab8666fc17b87724d1b9ef3932d23fTest
https://hal.inrae.fr/hal-04166029Test -
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المؤلفون: Catherine Allan, Penelope Cooke, Vaughan Higgins, Peat Leith, Melanie Bryant, Geoff Cockfield
المصدر: Outlook on Agriculture. 51:375-383
مصطلحات موضوعية: Ecology, Animal Science and Zoology, Agronomy and Crop Science
الوصف: This review seeks to understand the implications of using “adoption” to evaluate agricultural soil management outreach in the twenty-first century. The act of changing from one practice to another practice is referred to as “adoption”. The concept of adoption is closely associated with the design and evaluation of agricultural extension programs. Although focusing on adoption is deeply entrenched in agricultural extension, some scholars question the usefulness of the concept in light of the complexity and uncertainty that characterises farming in the twenty-first century. We present a purposeful review of literature that considers adoption in relation to three general approaches to agricultural extension; top-down, bottom-up and co-constructionist, with an emphasis on land management in Australia. The conceptual fit of adoption as a measure of success for each extension approach is explored. We conclude that the usefulness of adoption of individual practices or tools as a measure of success needs to be considered in context. Failing to reflect on what adoption means in any particular program or activity risks ignoring or misunderstanding real change and impacts and /or shaping activities to fit a simple, linear adoption expectation. We suggest that adoption remains a useful concept, but could be best considered as a gateway to increased reflection and reflexivity when projects and activities are being developed.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::924f9cc8393a9b067be7f2f50158f810Test
https://doi.org/10.1177/00307270221126540Test -
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المؤلفون: Melanie Bryant, Vaughan Higgins, Marta Hernández‐Jover, Russell Warman
المصدر: Australian Journal of Public Administration.
مصطلحات موضوعية: Public Administration, Sociology and Political Science
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::7852a95791e944bb63fe12bef3c908b8Test
https://doi.org/10.1111/1467-8500.12572Test -
5مؤتمر
المؤلفون: Russell Warman, Vaughan Higgins, Melanie Bryant, Hernandez-Jover, M
مصطلحات موضوعية: Forestry management and environment, Biosecurity science and invasive species ecology, Policy and administration not elsewhere classified, biosecurity, forestry, Australia, shared responsibility
الوصف: This presentation draws on research for the project Protecting Australia’s Food Future: Shared Responsibility for Biosecurity .
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6مؤتمر
المؤلفون: Melanie Bryant, Tobias Newstead, Joseph Crawford, Albert Amankwaa
مصطلحات موضوعية: Leadership, virtues, virtues-based leadership, leadership development
الوصف: No description available
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المؤلفون: Vaughan Higgins, Melanie Bryant
المصدر: Journal of Rural Studies. 81:315-323
مصطلحات موضوعية: Knowledge management, Sociology and Political Science, business.industry, Face value, Technological change, 05 social sciences, Geography, Planning and Development, 0211 other engineering and technologies, 0507 social and economic geography, 021107 urban & regional planning, 02 engineering and technology, Development, Empirical research, Resource (project management), Agriculture, Ontological security, Agricultural productivity, business, 050703 geography, Qualitative research
الوصف: Smart farming technologies are primarily associated with the transformation of agricultural productivity. Despite this, empirical research focusing on farm-level application of smart farming reveals a more complex and nuanced picture characterised by considerable uncertainty over its implementation and use. In this paper we seek to extend farm-level research by investigating two questions: how do perceived uncertainties destabilise meso-scale actors' routines and practices that are critical for ‘supporting farmer learning about the nature of digital data and its interpretation’ (Eastwood et al., 2019: 8); and, in what ways do meso-scale actors seek to re-establish a sense of stability and, in doing so, manage the uncertainty associated with smart farming implementation, and technological change more broadly? To address these questions we investigate the findings from a qualitative study of 20 meso-scale actors involved in the planning and implementation of smart farming technology in the Australian rice industry through an ontological security lens. We refer to meso-scale actors as farm advisors and agronomists whom we argue play a critical role in the uptake of smart farming technology. In applying this lens we argue that the perceived uncertainties related to smart farming de-stabilise or de-securitise actors' day-to-day roles and routines, impacting on who they are and what they do. We then demonstrate that actors draw upon two specific cultural scripts as a way to re-securitise their uncertainty. The first script seeks to securitise resource uncertainty by drawing upon known discourses surrounding farmer adoption of technology, while the second reproduces the importance of technologies that are easy to adopt while downplaying the importance of smart farming technology. While at face value these scripts can appear to create barriers to smart farming adoption, we argue that they can be a catalyst for developing solutions to uncertainty in terms of making smart farming more workable at the farm-level.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::dc1307be33a04ad622d5376640741a03Test
https://doi.org/10.1016/j.jrurstud.2020.10.051Test -
8دورية أكاديمية
المؤلفون: Allan, C, Cooke, P, Vaughan Higgins, Peat Leith, Melanie Bryant, Cockfield, G
مصطلحات موضوعية: Rural sociology, agricultural extension, innovation, knowledge co-construction, agricultural land management, adoption
الوصف: This review seeks to understand the implications of using “adoption” to evaluate agricultural soil management outreach in the twenty-first century. The act of changing from one practice to another practice is referred to as “adoption”. The concept of adoption is closely associated with the design and evaluation of agricultural extension programs. Although focusing on adoption is deeply entrenched in agricultural extension, some scholars question the usefulness of the concept in light of the complexity and uncertainty that characterises farming in the twenty-first century. We present a purposeful review of literature that considers adoption in relation to three general approaches to agricultural extension; top-down, bottom-up and co-constructionist, with an emphasis on land management in Australia. The conceptual fit of adoption as a measure of success for each extension approach is explored. We conclude that the usefulness of adoption of individual practices or tools as a measure of success needs to be considered in context. Failing to reflect on what adoption means in any particular program or activity risks ignoring or misunderstanding real change and impacts and /or shaping activities to fit a simple, linear adoption expectation. We suggest that adoption remains a useful concept, but could be best considered as a gateway to increased reflection and reflexivity when projects and activities are being developed.
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المؤلفون: Melanie Bryant, Vaughan Higgins
المصدر: Sociologia Ruralis. 60:438-457
مصطلحات موضوعية: Digital governance, Sociology and Political Science, Technological change, business.industry, 05 social sciences, 0211 other engineering and technologies, 0507 social and economic geography, 021107 urban & regional planning, 02 engineering and technology, Integrated approach, Ambivalence, Framing (social sciences), Sovereignty, Agriculture, Business, Technology implementation, 050703 geography, Industrial organization
الوصف: Meso-scale actors – such as farm advisors and other extension agents – are increasingly recognised as playing a critical role in managing farmer uncertainty associated with smart farming implementation. However, there has been limited research to date on how these actors navigate smart farming implementation within specific industries or commodity sectors. This article applies theoretical insights from the technological frames literature to investigate how meso-scale actors in the Australian rice industry frame smart farming technology implementation, and the ways in which relationships between frames variously afford and/or constrain industry sovereignty over implementation of technological change. Through analysis of our data, we reveal a complex relationship between participants’ technological frames involving frame incongruence, frame ambivalence and frame switching. We argue that while development of a more integrated approach to enhancing the digital capacities of meso-scale actors advocated in the literature provides a way of addressing frame incongruence, more flexible strategies may be needed to address frame ambivalence and switching. Furthermore, we contend that those frame relationships supporting industry sovereignty – namely frame ambivalence and switching – may not always be conducive to the promotion of digital agency among meso-scale actors, and could in fact impose limits on smart farming implementation.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::4cbce2e5317497e3bfdab5b417fb7be2Test
https://doi.org/10.1111/soru.12297Test -
10
المؤلفون: Melanie Bryant, Higgins
المصدر: Australian Journal of Management. 44:534-550
مصطلحات موضوعية: business.industry, 05 social sciences, Biosecurity, 0507 social and economic geography, Context (language use), Environmental ethics, Cognitive reframing, General Business, Management and Accounting, Agriculture, Political science, 0502 economics and business, Food processing, business, Shared responsibility, 050703 geography, 050203 business & management, Ambidexterity, Grand Challenges
الوصف: This article argues that an institutional logics framework is critical for developing a shared responsibility approach to managing the grand challenge of biosecurity in Australian agriculture. We identify the dominant logics evident in the Australian biosecurity context. In doing so, we draw attention to how a shared responsibility approach is compromised by tensions created by multiple logics, such as varying interpretations of biosecurity roles and responsibilities that different actors hold. However, in reframing such tensions from an institutional ambidexterity framework, we argue that a shared responsibility approach is achievable and, through examples from the Australian context, highlight the sites and spaces through which it may be fostered. We argue that identifying these sites and spaces requires that scholars conceptualise logics as blended rather than as discrete modes of operation. JEL Classification: M19
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::a36f4eb15c6fc91604d92ba7e35d6652Test
https://doi.org/10.1177/0312896219867997Test
النص الكامل