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1دورية أكاديمية
المصدر: Asian Pacific Journal of Cancer Biology, Vol 6, Iss 3, Pp 213-217 (2021)
مصطلحات موضوعية: bangladesh, hepatocellular carcinoma, mdm2, polymorphism, Biology (General), QH301-705.5
الوصف: Background: Hepatocellular carcinoma (HCC) is one of the fatal cancer types worldwide, and a variety of genetic factors are considered to be associated with this incidence. MDM2 gene plays a pivotal role in various pathways, which are essential to combat tumor formation. The study aimed to find out the associations of MDM2 (T309G, rs2279744) gene polymorphism with the development of HCC in the Bangladeshi population. Methods: A case-control study on 100 HCC patients and 110 control subjects was conducted. The genotyping of the MDM2 (T309G) gene was done using PCR-RFLP methods. Results: The percentage of TT and GG genotypes were significantly different (p0.05). In addition, patients having either GG or TG genotypes showed higher risk for HCC compared to control group (OR = 2.20; 95% CI = 1.21–4.14; P < 0.05). Conclusion: Our study suggested that the MDM2 gene may have a strong association with the development of HCC, and the GG allele could serve as an essential determinant to identify the higher risk of HCC in the Bangladeshi population.
وصف الملف: electronic resource
العلاقة: http://www.waocp.com/journal/index.php/apjcb/article/view/720Test; https://doaj.org/toc/2538-4635Test
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2دورية أكاديمية
المؤلفون: Md Mostafijur Rahman, Tamima Nawfal, Fabliha Afiea Khabir, Md Bayejid Hosen, Mubasshir Washif, Yearul Kabir, M Zakir Hossain Howlader
المصدر: Biochemistry and Biophysics Reports, Vol 35, Iss , Pp 101526- (2023)
مصطلحات موضوعية: GC gene, Preeclampsia, Polymorphism, VDR gene, Vitamin D, Biology (General), QH301-705.5, Biochemistry, QD415-436
الوصف: Objective: Preeclampsia is a multifactorial disease characterized by high blood pressure and protein in the urine. In this study, we investigated the association of vitamin D binding protein (GC) and vitamin D receptor (VDR) gene polymorphism with the risk of developing preeclampsia. Methods: 25-hydroxyvitamin D was measured using High-performance Liquid Chromatography. Vitamin D binding protein and vitamin D receptor gene polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism. Results: The control subjects have significant higher level of 25-hydroxyvitamin D (33.5 ± 1.194 ng/mL) relative to patients (23.97 ± 1.604 ng/mL) (p < 0.05). Vitamin D receptor rs1544410 and rs2228570 dominant model (GA + AA; TC + CC) showed significant higher risk of developing Preeclampsia (OR = 4.11, 95% CI = 0.62–27.09, p < 0.01; OR = 3.58, 95%CI = 0.78–16.38, p < 0.001 respectively). Similarly, vitamin D binding protein rs7041 and rs4588, dominant model (TG + GG; CA + AA) showed higher risk of preeclampsia development compared to control people (OR = 1.69, 95%CI = 0.35–8.19, p < 0.05; OR = 1.06, 95%CI = 0.25–4.44, p < 0.05 respectively). AA genotype of rs4588 of GC gene was significantly associated with 25-hydroxyvitamin D level in serum relative to CC and CA (p < 0.05). Conclusion: From our study, we can conclude that a low level of 25-hydroxyvitamin D, GC (rs1544410 and rs2228570), and VDR (rs4588 and rs7041) gene polymorphism is linked with an increased risk of developing preeclampsia.
العلاقة: http://www.sciencedirect.com/science/article/pii/S2405580823001073Test; https://doaj.org/toc/2405-5808Test; https://doaj.org/article/e79cc6638a38479ab71259dbda790d13Test
الإتاحة: https://doi.org/10.1016/j.bbrep.2023.101526Test
https://doaj.org/article/e79cc6638a38479ab71259dbda790d13Test -
3دورية أكاديمية
مصطلحات موضوعية: The p53 Signaling Network in Cancer Research, Oncology, Medicine, Health Sciences, RNA Methylation and Modification in Gene Expression, Molecular Biology, Biochemistry, Genetics and Molecular Biology, Life Sciences, Epigenetic Modifications and Their Functional Implications, MDM2, Hepatocellular carcinoma, Genotyping, Genotype, Internal medicine, Allele, Mdm2, Population, Gastroenterology, Biology, Gene, Genetics, FOS Biological sciences, Environmental health
الوصف: Background: Hepatocellular carcinoma (HCC) is one of the fatal cancer types worldwide, and a variety of genetic factors are considered to be associated with this incidence. MDM2 gene plays a pivotal role in various pathways, which are essential to combat tumor formation. The study aimed to find out the associations of MDM2 (T309G, rs2279744) gene polymorphism with the development of HCC in the Bangladeshi population. Methods: A case-control study on 100 HCC patients and 110 control subjects was conducted. The genotyping of the MDM2 (T309G) gene was done using PCR-RFLP methods. Results: The percentage of TT and GG genotypes were significantly different (p<0.01) among the study subjects. There were four genotyping groups, while the subjects with TT genotypes were considered the reference group. Patients with GG genotypes were at high risk of developing HCC (OR, 3.6; 95 % CI, 1.64–7.80; p<0.01) compared to the control. On the other hand, the association of TG genotypes with HCC was not statistically ... : خلفية: سرطان الخلايا الكبدية (HCC) هو أحد أنواع السرطان المميتة في جميع أنحاء العالم، وتعتبر مجموعة متنوعة من العوامل الوراثية مرتبطة بهذا الحدوث. يلعب جين MDM2 دورًا محوريًا في المسارات المختلفة، والتي تعتبر ضرورية لمكافحة تكوين الورم. هدفت الدراسة إلى معرفة ارتباطات تعدد أشكال الجينات MDM2 (T309G، rs2279744) مع تطور HCC في السكان البنغلاديشيين. الطرق: أجريت دراسة حالة على 100 مريض في مركز رأس المال البشري و 110 من الأشخاص الخاضعين للمراقبة. تم إجراء التنميط الجيني لجين MDM2 (T309G) باستخدام طرق PCR - RFLP. النتائج: كانت النسبة المئوية للأنماط الجينية TT و GG مختلفة بشكل كبير (p<0.01) بين المشاركين في الدراسة. كانت هناك أربع مجموعات تنميط وراثي، في حين أن الأشخاص الذين لديهم أنماط وراثية من TT كانوا يعتبرون المجموعة المرجعية. كان المرضى الذين يعانون من الأنماط الجينية لـ GG معرضين لخطر كبير للإصابة بـ HCC (OR، 3.6 ؛ 95 ٪ CI، 1.64-7.80 ؛ p<0.01) مقارنة بالتحكم. من ناحية أخرى، لم يكن ارتباط الأنماط الجينية لـ TG مع HCC ذا دلالة إحصائية (OR، 1.8 ؛ 95 ٪ CI، 0.91–3.40، p>0.05). بالإضافة إلى ذلك، أظهر ...
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4
المؤلفون: Mubasshir Washif, Tasnim Ahmad, Md Bayejid Hosen, Md Ratul Rahman, Tomoya Taniguchi, Hiromori Okubo, Kouji Hirota, Ryotaro Kawasumi
المصدر: DNA Repair. 127:103503
مصطلحات موضوعية: Cell Biology, Molecular Biology, Biochemistry
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::63ab87673bbec255bc0f8551b83bb21dTest
https://doi.org/10.1016/j.dnarep.2023.103503Test -
5دورية أكاديمية
المصدر: Iranian Journal of Reproductive Medicine, Vol 13, Iss 9, Pp 525-532 (2015)
مصطلحات موضوعية: DNA damage, Lipid peroxidation, Male infertility, Oxidative stress, Sperm quality., Gynecology and obstetrics, RG1-991, Reproduction, QH471-489
الوصف: Background: Sperm DNA damage is an important factor in the etiology of male infertility. Objective: The aim of the study was to evaluate the association of oxidative stress induced sperm DNA damage with the pathogenesis of male infertility. Materials and Methods: The study comprised a total of 66 subjects, including fertile men (n=25) and infertile men (n=41) matched by age. Seminal malondialdehyde (MDA), phospholipid hydroperoxide (PHP), superoxide dismutase (SOD), total antioxidant status (TAS) and 8-hydroxy-2'-deoxy guanosine (8-OHdG) were estimated by spectrophotometric and ELISA based methods and the association with the sperm parameters was assessed. Results: The percentages of motile and morphologically normal cells were significantly lower (p < 0.001, p
وصف الملف: electronic resource
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6
المصدر: Asian Pacific Journal of Cancer Biology. 6:213-217
مصطلحات موضوعية: Oncology, education.field_of_study, medicine.medical_specialty, business.industry, Population, Cancer, Building and Construction, medicine.disease, Polymorphism (computer science), Hepatocellular carcinoma, Internal medicine, Genotype, medicine, Gene polymorphism, Electrical and Electronic Engineering, Allele, education, business, Genotyping
الوصف: Background: Hepatocellular carcinoma (HCC) is one of the fatal cancer types worldwide, and a variety of genetic factors are considered to be associated with this incidence. MDM2 gene plays a pivotal role in various pathways, which are essential to combat tumor formation. The study aimed to find out the associations of MDM2 (T309G, rs2279744) gene polymorphism with the development of HCC in the Bangladeshi population. Methods: A case-control study on 100 HCC patients and 110 control subjects was conducted. The genotyping of the MDM2 (T309G) gene was done using PCR-RFLP methods. Results: The percentage of TT and GG genotypes were significantly different (p0.05). In addition, patients having either GG or TG genotypes showed higher risk for HCC compared to control group (OR = 2.20; 95% CI = 1.21–4.14; P < 0.05). Conclusion: Our study suggested that the MDM2 gene may have a strong association with the development of HCC, and the GG allele could serve as an essential determinant to identify the higher risk of HCC in the Bangladeshi population.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::4bdd37c8d10b03c551c3f73690f22f3eTest
https://doi.org/10.31557/apjcb.2021.6.3.213-217Test -
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المؤلفون: Mohammad Fazle Alam Rabbi, Mala Khan, Mamudul Hasan Razu, Md. Imran Khan, Saam Hasan, Mauricio Chalita, Kazi Nadim Hasan, Abu Sufian, Md. Bayejid Hosen, Mohammed Nafiz Imtiaz Polol, Jannatun Naima, Kihyun Lee, Yeong Ouk Kim, Md. Mizanur Rahman, Jongsik Chun, Md. Abdul Khaleque, Zabed Bin Ahmed, Nur A. Hasan, Rita R. Colwell, Sharif Akhteruzzaman
المصدر: SSRN Electronic Journal.
مصطلحات موضوعية: History, Polymers and Plastics, Genetics, Business and International Management, Industrial and Manufacturing Engineering
الوصف: The goal of this study was to identify the genomic variants and determine molecular epidemiology of SARS-CoV-2 virus during the early pandemic stage in Bangladesh. Viral RNA was extracted, converted to cDNA, and amplified using Ion AmpliSeq™ SARS-CoV-2 Research Panel. 413 unique mutants from 151 viral isolates were identified. 80% of cases belongs to 8 mutants: 241C toT, 1163A toT, 3037C toT, 14408C toT, 23403A toG, 28881G toA, 28,882 G toA, and 28883G toC. Observed dominance of GR clade variants that have strong presence in Europe, suggesting European channel a possible entry route. Among 37 genomic mutants significantly associated with clinical symptoms, 3916CtoT (associated with sore-throat), 14408C to T (associated with cough-protection), 28881G to A, 28882G to A, and 28883G to C (associated with chest pain) were notable. These findings may inform future research platforms for disease management and epidemiological study.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f80386c2d01b23c3262fe772f6285d3Test
https://doi.org/10.2139/ssrn.4125380Test -
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المصدر: Current Nutrition & Food Science. 16:815-823
مصطلحات موضوعية: 0303 health sciences, Nutrition and Dietetics, Dietary exposure, Public Health, Environmental and Occupational Health, Heavy metals, 010501 environmental sciences, 01 natural sciences, Trace (semiology), 03 medical and health sciences, Environmental chemistry, Environmental science, 030304 developmental biology, 0105 earth and related environmental sciences, Food Science
الوصف: Background: Contamination of soil and agricultural products by heavy metals resulting from rapid industrial development has caused major concern. Dietary exposure to heavy metals has been associated with toxic and adverse health effects. The main threats to human health from heavy metals are associated with exposure to Pb, Cd and Hg. The aim of this study was to monitor the presence of heavy metals, minerals and trace elements in cereals consumed by Dhaka city residents. Methods: One hundred and sixty cereal samples were collected for eight (08) composited samples and analyzed for the determination of sixteen elements. Heavy metals were analyzed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), and other elements were determined by Atomic Absorption Spectrometry (AAS). Results: The average concentrations of elements in cereals fall within the safe limit except for Pb, Cr, Na and Mg, which exceeded the safe limits. The daily intake of Cd (23.0 μg), Hg (63.0 μg) and As (13.6 μg) through cereals was below the risk level except for Pb (634.0 μg) and Cr (263.1 μg). The dietary intake of Na (1860.0 mg), Mg (347.0 mg) and Mn (4600.0 μg) exceeded the toxic level while K (829 mg) was below the RDA. Conclusion: As the main meal of average Bangladeshi people is boiled rice served with some vegetables, our findings indicate that the residents of Dhaka city are at risk from Pb and Cr contamination and their dietary need for some important minerals and trace elements is not fulfilled by cereals.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::394d6e0c618952083fd8fddcdaea4624Test
https://doi.org/10.2174/1573401315666191113154200Test -
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المؤلفون: Jannatun Naima, Md. Bayejid Hosen, Mohammad Fazle Alam Rabbi, Jongsik Chun, Mohammed Nafiz Imtiaz Polol, Kazi Nadim Hasan, Md. Abdul Khaleque, Kihyun Lee, Saam Hasan, Md. Mizanur Rahman, Yeong Ouk Kim, Mamudul Hasan Razu, Md. Imran H. Khan, Nur A. Hasan, Mauricio Chalita, Sharif Akhteruzzaman, Mala Khan, Abu Sufian, Rita R. Colwell
مصطلحات موضوعية: Whole genome sequencing, Genetics, Massive parallel sequencing, Molecular epidemiology, Biology, Clade, Genome, Virus, Genetic association, Reference genome
الوصف: RationaleThe global public health is in serious crisis due to emergence of SARS-CoV-2 virus. Studies are ongoing to reveal the genomic variants of the virus circulating in various parts of the world. However, data generated from low- and middle-income countries are scarce due to resource limitation. This study was focused to perform whole genome sequencing of 151 SARS-CoV-2 isolates from COVID-19 positive Bangladeshi patients. The goal of this study was to identify the genomic variants among the SARS-CoV-2 virus isolates in Bangladesh, to determine the molecular epidemiology and to develop a relationship between host clinical trait with the virus genomic variants.MethodSuspected patients were tested for COVID-19 using one step commercial qPCR kit for SARS-CoV-2 Virus. Viral RNA was extracted from positive patients, converted to cDNA which was amplified using Ion AmpliSeq™ SARS-CoV-2 Research Panel. Massive parallel sequencing was carried out using Ion AmpliSeq™ Library Kit Plus. Assembly of raw data is done by aligning the reads to a pre-defined reference genome (NC_045512.2) while retaining the unique variations of the input raw data by creating a consensus genome. A random forest-based association analysis was carried out to correlate the viral genomic variants with the clinical traits present in the host.ResultAmong the 151 viral isolates, we observed the 413 unique variants. Among these 8 variants occurred in more than 80 % of cases which include 241C to T, 1163A to T, 3037C to T,14408C to T, 23403A to G, 28881G to A, 28882 G to A, and finally the 28883G to C. Phylogenetic analysis revealed a predominance of variants belonging to GR clade, which have a strong geographical presence in Europe, indicating possible introduction of the SARS-CoV-2 virus into Bangladesh through a European channel. However, other possibilities like a route of entry from China cannot be ruled out as viral isolate belonging to L clade with a close relationship to Wuhan reference genome was also detected. We observed a total of 37 genomic variants to be strongly associated with clinical symptoms such as fever, sore throat, overall symptomatic status, etc. (Fisher’s Exact Test p-valueConclusionTo our knowledge, this study is the first large scale phylogenomic studies of SARS-CoV-2 virus circulating in Bangladesh. The observed epidemiological and genomic features may inform future research platform for disease management, vaccine development and epidemiological study.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::3527a696c3076b52ecdde0e554a10032Test
https://doi.org/10.1101/2021.01.17.425424Test -
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المؤلفون: Md. Mehedi Hasan, Md. Omar Faruk, Md. Mostafijur Rahman, Md. Bayejid Hosen, Yearul Kabir, M Zakir Hossain Howlader
المصدر: Gene. 636:42-47
مصطلحات موضوعية: Adult, Male, 0301 basic medicine, Vitamin, medicine.medical_specialty, Genotype, Vitamin D-binding protein, 030209 endocrinology & metabolism, Single-nucleotide polymorphism, Type 2 diabetes, Biology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Blood serum, Internal medicine, Genetics, medicine, Vitamin D and neurology, Humans, Genetic Predisposition to Disease, Vitamin D, Bangladesh, Polymorphism, Genetic, Vitamin D-Binding Protein, Type 2 Diabetes Mellitus, General Medicine, medicine.disease, 030104 developmental biology, Endocrinology, Diabetes Mellitus, Type 2, Haplotypes, chemistry, Female, Gene polymorphism
الوصف: Polymorphism in vitamin D binding protein gene may have an impact on serum vitamin D transport and thus may have relation with type 2 diabetes mellitus. In our study, we investigated the association of serum vitamin D level and vitamin D-binding protein gene polymorphism with the onset of type 2 diabetes mellitus.Blood samples were collected from 104 type 2 diabetic patients and 107 healthy volunteers. Serum vitamin D was measured by high-performance liquid chromatography. Genetic analysis of vitamin D-binging protein gene was carried out by polymerase chain reaction - restriction fragment length polymorphism method.We found significantly (p0.001) lower level of vitamin D in type 2 diabetic patients compared to control subjects. A significantly negative correlation (r=-0.25, p0.05) between vitamin D level and fasting blood glucose level was found among type 2 diabetic subjects. The Glu/Glu at codon 416 (rs7041) (p0.05) and Lys/Lys at codon 420 (rs4588) (p0.01) variants of vitamin D binding protein gene was significantly higher in type 2 diabetic subjects than controls. The patients with Glu/Glu and Lys/Lys genotypes respectively at codon 416 (odds ratio=2.87; 95% confidence interval=1.19 to 6.95) and 420 (odds ratio=8.9; 95% confidence interval=1.89 to 41.99) were at high risk of developing type 2 diabetes.Our present study strongly suggests that there might have an association of vitamin D, and vitamin D-binding protein gene (codon 416420) polymorphisms with the occurrence of type 2 diabetes mellitus.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ecf639e807dbc83f818041ea9d127b4Test
https://doi.org/10.1016/j.gene.2017.09.008Test
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