المؤلفون: François-Xavier Briand, Eric Niqueux, Audrey Schmitz, Claire Martenot, Martine Cherbonnel, Pascale Massin, Florian Kerbrat, Marina Chatel, Carole Guillemoto, Cecile Guillou-Cloarec, Katell Ogor, Aurélie Le Prioux, Chantal Allée, Véronique Beven, Edouard Hirchaud, Yannick Blanchard, Axelle Scoizec, Sophie Le Bouquin, Nicolas Eterradossi, Béatrice Grasland

المصدر: Emerging Infectious Diseases, Vol 27, Iss 2, Pp 508-516 (2021)

مصطلحات موضوعية: epizootic, H5N8, evolution, highly pathogenic, influenza, avian influenza, Medicine, Infectious and parasitic diseases, RC109-216

الوصف: We detected 3 genotypes of highly pathogenic avian influenza A(H5N8) virus in France during winter 2016–17. Genotype A viruses caused dramatic economic losses in the domestic duck farm industry in southwestern France. Our phylogenetic analysis suggests that genotype A viruses formed 5 distinct geographic clusters in southwestern France. In some clusters, local secondary transmission might have been started by a single introduction. The intensity of the viral spread seems to correspond to the density of duck holdings in each production area. To avoid the introduction of disease into an unaffected area, it is crucial that authorities limit the movements of potentially infected birds.

وصف الملف: electronic resource

العلاقة: https://wwwnc.cdc.gov/eid/article/27/2/20-2920_articleTest; https://doaj.org/toc/1080-6040Test; https://doaj.org/toc/1080-6059Test

الوصول الحر: https://doaj.org/article/12fff99b7dbd491b83b445879a01b1fdTest

المؤلفون: Adeline Huneau-Salaün, Axelle Scoizec, Rodolphe Thomas, Claire Martenot, Audrey Schmitz, Isabelle Pierre, Chantal Allée, Rachel Busson, Pascale Massin, François-Xavier Briand, Carole Guillemoto, Katell Louboutin, Florent Souchaud, Martine Cherbonnel-Pansart, Eric Niqueux, Béatrice Grasland, Rozenn Souillard, Sophie Le Bouquin

المصدر: Poultry Science, Vol 101, Iss 1, Pp 101569- (2022)

مصطلحات موضوعية: avian influenza, transport, cleaning, disinfection, biosecurity, Animal culture, SF1-1100

الوصف: ABSTRACT: In 2021, France faced large avian influenza outbreaks, like in 2016 and 2017. Controlling these outbreaks required the preventive depopulation of a large number of duck farms. A previous study in 2017 showed that the quality of decontamination of trucks and transport crates used for depopulation was often insufficient. A new study was then set up to evaluate cleaning and disinfection (C&D) of trucks and crates used for duck depopulation and whether practices had changed since 2017. Three methods were used to assess decontamination: 1) detection of avian influenza virus (AIV) genome, 2) visual inspection of cleanliness, and 3) microbial counts, considering that 2 and 3 are commonly used in abattoirs. Another objective of the study was to evaluate the correlation between results obtained with the 3 methods. In 5 abattoirs, 8 trucks and their crates were sampled by swabbing to detect AIV genome by rRT-PCR before and after decontamination. Visual cleanliness scores and coliform counts were also determined on crates after C&D. Trucks and crates were decontaminated according to the abattoirs’ protocols. Before C&D, 3 quarters of crates (59/79) and 7 of 8 trucks were positive for AIV genome. C&D procedures were reinforced in 2021 compared to 2017; use of detergent solution and warm water were more common. Nevertheless, 28% of the crates were positive for AIV genome after C&D, despite the fact that cleaning scores and microbiological counts were satisfactory for 84% and 91% of the crates, respectively. No correlation was observed between results for AIV genome detection and results from visual control or from coliform counts. Abattoirs are encouraged to use environmental sampling coupled with AIV genome detection to monitor the quality of cleaning and disinfection of trucks and crates during AI outbreaks. Reinforcement of biosecurity measures at abattoirs is still needed to avoid residual contamination of the equipment and cross-contamination during the decontamination process.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S0032579121005903Test; https://doaj.org/toc/0032-5791Test

الوصول الحر: https://doaj.org/article/7637c286b64942efabe64fa29bea8d3dTest

المؤلفون: Cyril Le Nouën, Didier Toquin, Hermann Müller, Rüdiger Raue, Katherine M Kean, Patrick Langlois, Martine Cherbonnel, Nicolas Eterradossi

المصدر: PLoS ONE, Vol 7, Iss 1, p e28064 (2012)

مصطلحات موضوعية: Medicine, Science

الوصف: BACKGROUND: Infectious bursal disease virus (IBDV) is a pathogen of worldwide significance to the poultry industry. IBDV has a bi-segmented double-stranded RNA genome. Segments A and B encode the capsid, ribonucleoprotein and non-structural proteins, or the virus polymerase (RdRp), respectively. Since the late eighties, very virulent (vv) IBDV strains have emerged in Europe inducing up to 60% mortality. Although some progress has been made in understanding the molecular biology of IBDV, the molecular basis for the pathogenicity of vvIBDV is still not fully understood. METHODOLOGY, PRINCIPAL FINDINGS: Strain 88180 belongs to a lineage of pathogenic IBDV phylogenetically related to vvIBDV. By reverse genetics, we rescued a molecular clone (mc88180), as pathogenic as its parent strain. To study the molecular basis for 88180 pathogenicity, we constructed and characterized in vivo reassortant or mosaic recombinant viruses derived from the 88180 and the attenuated Cu-1 IBDV strains. The reassortant virus rescued from segments A of 88180 (A88) and B of Cu-1 (BCU1) was milder than mc88180 showing that segment B is involved in 88180 pathogenicity. Next, the exchange of different regions of BCU1 with their counterparts in B88 in association with A88 did not fully restore a virulence equivalent to mc88180. This demonstrated that several regions if not the whole B88 are essential for the in vivo pathogenicity of 88180. CONCLUSION, SIGNIFICANCE: The present results show that different domains of the RdRp, are essential for the in vivo pathogenicity of IBDV, independently of the replication efficiency of the mosaic viruses.

وصف الملف: electronic resource

العلاقة: http://europepmc.org/articles/PMC3258228?pdf=renderTest; https://doaj.org/toc/1932-6203Test

الوصول الحر: https://doaj.org/article/fe179aab92034c27a3604fe9a297b5f2Test

المؤلفون: François‐Xavier Briand, Eric Niqueux, Audrey Schmitz, Claire Martenot, Martine Cherbonnel, Pascale Massin, Rachel Busson, Carole Guillemoto, Isabelle Pierre, Katell Louboutin, Florent Souchaud, Chantal Allée, Helene Quenault, Pierrick Lucas, Anne Van de Wiele, Yannick Blanchard, Nicolas Eterradossi, Axelle Scoizec, Sophie Le Bouquin‐Leneveu, Severine Rautureau, Yves Lambert, Béatrice Grasland

المصدر: Transboundary and Emerging Diseases. 69:4028-4033

مصطلحات موضوعية: General Veterinary, General Immunology and Microbiology, General Medicine

الوصف: During winter 2020-2021, France and other European countries were severely affected by highly pathogenic avian influenza H5 viruses of the Gs/GD/96 lineage, clade 2.3.4.4b. In total, 519 cases occurred, mainly in domestic waterfowl farms in Southwestern France. Analysis of viral genomic sequences indicated that 3 subtypes of HPAI H5 viruses were detected (H5N1, H5N3, H5N8), but most French viruses belonged to the H5N8 subtype genotype A, as Europe. Phylogenetic analyses of HPAI H5N8 viruses revealed that the French sequences were distributed in 9 genogroups, suggesting 9 independent introductions of H5N8 from wild birds, in addition to the 2 introductions of H5N1 and H5N3.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01ac194e358c4dda61dc71d118a15dbfTest
https://doi.org/10.1111/tbed.14711Test

المؤلفون: Éric Niqueux, Marion Flodrops, Chantal Allée, Marie-Odile Lebras, Isabelle Pierre, Katell Louboutin, Carole Guillemoto, Aurélie Le Prioux, Sophie Le Bouquin-Leneveu, Alassane Keïta, Michel Amelot, Claire Martenot, Pascale Massin, Martine Cherbonnel-Pansart, François-Xavier Briand, Audrey Schmitz, Christophe Cazaban, Gwenaëlle Dauphin, Thomas Delquigny, Stéphane Lemière, Jean-Marie Watier, Mark Mogler, Ian Tarpey, Béatrice Grasland, Nicolas Eterradossi

المصدر: Vaccine.

مصطلحات موضوعية: Infectious Diseases, General Veterinary, General Immunology and Microbiology, Public Health, Environmental and Occupational Health, Molecular Medicine

الوصف: In France during winter 2016-2017, 487 outbreaks of clade 2.3.4.4b H5N8 subtype high pathogenicity (HP) avian influenza A virus (AIV) infections were detected in poultry and captive birds. During this epizootic, HPAIV A/decoy duck/France/161105a/2016 (H5N8) was isolated and characterized in an experimental infection transmission model in conventional mule ducks. To investigate options to possibly protect such ducks against this HPAIV, three vaccines were evaluated in controlled conditions. The first experimental vaccine was derived from the hemagglutinin gene of another clade 2.3.4.4b A(H5N8) HPAIV. It was injected at three weeks of age, either alone (Vac1) or after a primer injection at day-old (Vac1 + boost). The second vaccine (Vac2) was a commercial bivalent adjuvanted vaccine containing an expressed hemagglutinin modified from a clade 2.3.2 A(H5N1) HPAIV. Vac2 was administered as a single injection at two weeks of age. The third experimental vaccine (Vac3) also incorporated a homologous 2.3.4.4b H5 HA gene and was administered as a single injection at three weeks of age. Ducks were challenged with HPAIV A/decoy duck/France/161105a/2016 (H5N8) at six weeks of age. Post-challenge virus excretion was monitored in vaccinated and control birds every 2-3 days for two weeks using real-time reverse-transcription polymerase chain reaction and serological analyses (haemagglutination inhibition test against H5N8, H5 ELISA and AIV ELISA) were performed. Vac1 abolished oropharyngeal and cloacal shedding to almost undetectable levels, whereas Vac3 abolished cloacal shedding only (while partially reducing respiratory shedding) and Vac2 only partly reduced the respiratory and intestinal excretion of the challenge virus. These results provided relevant insights in the immunogenicity of recombinant H5 vaccines in mule ducks, a rarely investigated hybrid between Pekin and Muscovy duck species that has played a critical role in the recent H5 HPAI epizootics in France.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad62bf447db9fca6b40d9744e064b6c1Test
https://pubmed.ncbi.nlm.nih.gov/36411134Test

المؤلفون: François-Xavier Briand, Audrey Schmitz, Axelle Scoizec, Chantal Allée, Rachel Busson, Carole Guillemoto, Hélène Quenault, Pierrick Lucas, Isabelle Pierre, Katell Louboutin, Cécile Guillou-Cloarec, Claire Martenot, Martine Cherbonnel-Pansart, Rodolphe Thomas, Pascale Massin, Florent Souchaud, Yannick Blanchard, Mieke Steensels, Benedicte Lambrecht, Nicolas Eterradossi, Sophie Le Bouquin, Eric Niqueux, Béatrice Grasland

المصدر: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 104

مصطلحات موضوعية: Microbiology (medical), Infectious Diseases, Influenza A virus, Influenza in Birds, Genetics, Animals, Female, Molecular Biology, Microbiology, Chickens, Ecology, Evolution, Behavior and Systematics, Phylogeny

الوصف: An H3N1 avian influenza virus was detected in a laying hens farm in May 2019 which had experienced 25% mortality in Northern France. The complete sequencing of this virus showed that all segment sequences belonged to the Eurasian lineage and were phylogenetically very close to many of the Belgian H3N1 viruses detected in 2019. The French virus presented two genetic particularities with NA and NS deletions that could be related to virus adaptation from wild to domestic birds and could increase virulence, respectively. Molecular data of H3N1 viruses suggest that these two deletions occurred at two different times.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66c816dc9d3aa5e9c4dfddd77c363b5bTest
https://pubmed.ncbi.nlm.nih.gov/36038008Test

المؤلفون: Audrey Schmitz, François-Xavier Briand, Florian Kerbrat, Eric Niqueux, Yannick Blanchard, Marina Chatel, Béatrice Grasland, Chantal Allée, Véronique Béven, Katell Ogor, Axelle Scoizec, Carole Guillemoto, Sophie Le Bouquin, Martine Cherbonnel, Nicolas Eterradossi, Edouard Hirchaud, Pascale Massin, Cécile Guillou-Cloarec, Aurélie Le Prioux, Claire Martenot

المساهمون: Laboratoire de Ploufragan-Plouzané-Niort [ANSES], Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Fonds de la Recherche pour l’Influenza Aviaire 2019 from the French Ministry of Agriculture

المصدر: Emerging Infectious Diseases, Vol 27, Iss 2, Pp 508-516 (2021)
Emerging Infectious Diseases
Emerging Infectious Diseases, Centers for Disease Control and Prevention, 2021, 27 (2), pp.508-516. ⟨10.3201/eid2702.202920⟩

مصطلحات موضوعية: Epidemiology, Range (biology), viruses, highly pathogenic, lcsh:Medicine, medicine.disease_cause, epizootic, Disease Outbreaks, law.invention, 0302 clinical medicine, law, Genotype, Pathogenic Avian Influenza A(H5N8) Virus Spread by Short- and Long-Range Transmission, France, 2016–17, MESH: Influenza A Virus, H5N8 Subtype, Influenza A Virus, H5N8 Subtype, 030212 general & internal medicine, MESH: Phylogeny, Phylogeny, Phylogenetic tree, transmission, Infectious Diseases, Transmission (mechanics), MESH: Birds, France, influenza, Microbiology (medical), Highly pathogenic, 030231 tropical medicine, Zoology, Animals, Wild, Biology, Virus, lcsh:Infectious and parasitic diseases, Birds, 03 medical and health sciences, MESH: Influenza in Birds, H5N8, genotypes, evolution, medicine, Animals, MESH: Animals, Wild, lcsh:RC109-216, highly pathogenic avian influenza, Epizootic, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, viral zoonoses, Research, geoclusters, lcsh:R, medicine.disease, Influenza A virus subtype H5N1, zoonoses, MESH: France, Influenza in Birds, avian influenza

الوصف: International audience; We detected 3 genotypes of highly pathogenic avian influenza A(H5N8) virus in France during winter 2016–17. Genotype A viruses caused dramatic economic losses in the domestic duck farm industry in southwestern France. Our phylogenetic analysis suggests that genotype A viruses formed 5 distinct geographic clusters in southwestern France. In some clusters, local secondary transmission might have been started by a single introduction. The intensity of the viral spread seems to correspond to the density of duck holdings in each production area. To avoid the introduction of disease into an unaffected area, it is crucial that authorities limit the movements of potentially infected birds.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c54e5843d3062b84e399219a725ca23fTest
https://doi.org/10.3201/eid2702.202920Test

المؤلفون: Adeline Huneau-Salaün, Axelle Scoizec, Rodolphe Thomas, Claire Martenot, Audrey Schmitz, Isabelle Pierre, Chantal Allée, Rachel Busson, Pascale Massin, François-Xavier Briand, Carole Guillemoto, Katell Louboutin, Florent Souchaud, Martine Cherbonnel-Pansart, Eric Niqueux, Béatrice Grasland, Rozenn Souillard, Sophie Le Bouquin

المصدر: Poultry Science
Poultry Science, Vol 101, Iss 1, Pp 101569-(2022)

مصطلحات موضوعية: Disinfection, Biosecurity, Influenza in Birds, IMMUNOLOGY, HEALTH AND DISEASE, transport, cleaning, Animals, avian influenza, SF1-1100, Chickens, Animal culture, Disease Outbreaks

الوصف: In 2021, France faced large avian influenza outbreaks, like in 2016 and 2017. Controlling these outbreaks required the preventive depopulation of a large number of duck farms. A previous study in 2017 showed that the quality of decontamination of trucks and transport crates used for depopulation was often insufficient. A new study was then set up to evaluate cleaning and disinfection (C&D) of trucks and crates used for duck depopulation and whether practices had changed since 2017. Three methods were used to assess decontamination: 1) detection of avian influenza virus (AIV) genome, 2) visual inspection of cleanliness, and 3) microbial counts, considering that 2 and 3 are commonly used in abattoirs. Another objective of the study was to evaluate the correlation between results obtained with the 3 methods. In 5 abattoirs, 8 trucks and their crates were sampled by swabbing to detect AIV genome by rRT-PCR before and after decontamination. Visual cleanliness scores and coliform counts were also determined on crates after C&D. Trucks and crates were decontaminated according to the abattoirs’ protocols. Before C&D, 3 quarters of crates (59/79) and 7 of 8 trucks were positive for AIV genome. C&D procedures were reinforced in 2021 compared to 2017; use of detergent solution and warm water were more common. Nevertheless, 28% of the crates were positive for AIV genome after C&D, despite the fact that cleaning scores and microbiological counts were satisfactory for 84% and 91% of the crates, respectively. No correlation was observed between results for AIV genome detection and results from visual control or from coliform counts. Abattoirs are encouraged to use environmental sampling coupled with AIV genome detection to monitor the quality of cleaning and disinfection of trucks and crates during AI outbreaks. Reinforcement of biosecurity measures at abattoirs is still needed to avoid residual contamination of the equipment and cross-contamination during the decontamination process.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::6e8a396401ee9868e510efef11cecd0cTest
http://europepmc.org/articles/PMC8626697Test

المؤلفون: Aurélie Le Prioux, Evelyne Lemaitre, Edouard Hirchaud, Chantal Allée, Sylvie van der Werf, Martine Cherbonnel-Pansart, Fabrice Touzain, Hélène Quenault, Nicolas Eterradossi, Carole Guillemoto, Véronique Jestin, Alexandre Fediaevsky, Marie-Odile Le Bras, Hélène Gares, Pascale Massin, Yannick Blanchard, Eric Niqueux, Céline Courtillon, Katell Ogor, François-Xavier Briand, Audrey Schmitz, Patrick Daniel, Cécile Guillou-Cloarec

المساهمون: Laboratoire de Ploufragan-Plouzané-Niort [ANSES], Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université Bretagne Loire (UBL), Laboratoire départemental d'analyse et de recherche de Dordogne (LDAR 24), Conseil départemental de la Dordogne - Service de l'archéologie, Laboratoires des Pyrénées et des Landes, Direction Générale de l'Alimentation (DGAL), Ministère de l'agriculture, de l'agroalimentaire et de la forêt, Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses (GMV-ARN (UMR_3569 / U-Pasteur_2)), Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS)

المصدر: Eurosurveillance
Eurosurveillance, 2017, 22 (9), ⟨10.2807/1560-7917.ES.2017.22.9.30473⟩

مصطلحات موضوعية: 0301 basic medicine, Genes, Viral, Epidemiology, animal diseases, [SDV]Life Sciences [q-bio], highly pathogenic, Hemagglutinin Glycoproteins, Influenza Virus, Avian influenza, medicine.disease_cause, phylogeny, Polymerase Chain Reaction, Genome, Poultry, Disease Outbreaks, law.invention, 0403 veterinary science, law, Pandemic, Polymerase chain reaction, Genetics, Phylogenetic tree, virus diseases, 04 agricultural and veterinary sciences, Ducks, H5, Zoonotic Potential, RNA, Viral, France, Influenza A Virus, H5N2 Subtype, Research Article, 040301 veterinary sciences, Molecular Sequence Data, Virulence, Biology, Virus, Birds, 03 medical and health sciences, Phylogenetics, Virology, medicine, Animals, Humans, Poultry Diseases, Influenza A Virus, H5N1 Subtype, Public Health, Environmental and Occupational Health, Sequence Analysis, DNA, Influenza A virus subtype H5N1, 030104 developmental biology, Influenza in Birds, Chickens

الوصف: International audience; Several new highly pathogenic (HP) H5 avian influenza virus (AIV) have been detected in poultry farms from south-western France since November 2015, among which an HP H5N1. The zoonotic potential and origin of these AIVs immediately became matters of concern. One virus of each subtype H5N1 (150169a), H5N2 (150233) and H5N9 (150236) was characterised. All proved highly pathogenic for poultry as demonstrated molecularly by the presence of a polybasic cleavage site in their HA protein – with a sequence (HQRRKR/GLF) previously unknown among avian H5 HPAI viruses – or experimentally by the in vivo demonstration of an intravenous pathogenicity index of 2.9 for the H5N1 HP isolate. Phylogenetic analyses based on the full genomes obtained by NGS confirmed that the eight viral segments of the three isolates were all part of avian Eurasian phylogenetic lineage but differed from the Gs/Gd/1/96-like lineage. The study of the genetic characteristics at specific amino acid positions relevant for modulating the adaptation to and the virulence for mammals showed that presently, these viruses possess most molecular features characteristic of AIV and lack some major characteristics required for efficient respiratory transmission to or between humans. The three isolates are therefore predicted to have no significant pandemic potential.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8920a5ff1e7b52b63f34ef56e89d51fcTest
https://hal-pasteur.archives-ouvertes.fr/pasteur-04097764/documentTest

المؤلفون: Anne Prel, Ghislaine Le Gall-Reculé, Martine Cherbonnel, Béatrice Grasland, Michel Amelot, Véronique Jestin

المساهمون: Anne Prel, Ghislaine Le Gall-Reculé, Martine Cherbonnel, Béatrice Grasland, Michel Amelot, Véronique Jestin

المصدر: https://doi.org/10.1637/1933-5334Test(2007)2[e69:AOTPAB]2.0.CO;2.

جغرافية الموضوع: world

وصف الملف: text/HTML

الإتاحة: https://doi.org/10.1637/1933-5334Test(2007)2[e69:AOTPAB]2.0.CO;2