المؤلفون: Cardenas-Cadena, Sergio Andres, Castañeda-Lopez, Maria Eugenia, Mollinedo-Montaño, Fabiana Esther, Vazquez-Reyes, Sodel, Lara-Arias, Jorge, Marino-Martinez, Ivan Alberto, Rodriguez-Sanchez, Iram Pablo, Garza-Veloz, Idalia, Martinez-Fierro, Margarita L.

المساهمون: Molecular Medicine Laboratory, Universidad Autónoma de Zacatecas

المصدر: Acta Parasitologica ; volume 68, issue 3, page 705-710 ; ISSN 1230-2821 1896-1851

مصطلحات موضوعية: Parasitology

الوصف: Purpose This study aims to develop and evaluate a cost-effective, user-friendly multiplex quantitative real-time polymerase chain reaction (qPCR) method for detecting multiple tick-borne pathogens associated with human and veterinary diseases. Methods In silico PCR was performed to design and evaluate primer sequences reported for amplifying Rickettsia spp ., Borrelia spp. , and Ehrlichia spp. Single and multiplex qPCR assays were then standardized to detect individual pathogens and multiple pathogens in a single reaction. Positive controls were generated to determine the dynamic range of the methods. In the validation phase, a total of 800 samples were screened for the presence of tick-borne pathogens. Results Identification in a single qPCR reaction (multiplex) of Ehrlichia spp . , and Borrelia spp. with a limit of detection of 10 copies and Rickettsia spp . with 100 copies, a PCR efficiency (E) of 90–100% and a coefficient of correlation ( R 2 ) of 0.998–0.996 for all pathogens. Conclusion The ability to detect three significant pathogens (Ehrlichia spp ., Rickettsia spp ., and Borrelia spp .) in a single qPCR reaction offers a significant advantage in the field of molecular diagnostics for tick-borne diseases. This advancement has a profound impact on public health as it facilitates the selection of appropriate treatment protocols, thereby reducing complications associated with disease progression. The streamlined approach provided by this method simplifies the diagnostic process and enables timely intervention, ultimately improving patient outcomes and mitigating the potential risks associated with untreated or misdiagnosed tick-borne infections.

الإتاحة: https://doi.org/10.1007/s11686-023-00702-0Test

المؤلفون: Abellan-Borja, Andres, Rodriguez-Sanchez, Iram P., Carrera-Treviño, Rogelio, Villanueva-Segura, Olga Karina, Zapata-Morin, Patricio Adrian, Martinez-de-Villareal, Laura E., Barboza-Aranda, Luis Javier, Gomez-Govea, Mayra A., Martinez-Fierro, Margarita L., Delgado-Enciso, Ivan, Ruiz-Ayma, Gabriel, Gonzalez-Rojas, Jose Ignacio, Guzman-Velasco, Antonio

المساهمون: Giroud, Sylvain

المصدر: PLOS ONE ; volume 18, issue 2, page e0272979 ; ISSN 1932-6203

الوصف: Introduction Ursus americanus Pallas 1780 is the largest carnivore and the only ursid in Mexico. It is considered an endangered species in the country because its distribution and population have been reduced by up to 80% because of habitat loss or furtive hunting. These problems can lead to a diet change, which could result in metabolic disorders, such as fatty acid β-oxidation defects or organic acid metabolism disorders. In our study, a free amino acid and acylcarnitine profile was characterized. Methods Peripheral blood samples were drawn from nine free-ranging black bears in a period of five months, from June to October of 2019 in Northeastern Mexico, and 12 amino acids and 30 acylcarnitines were determined and quantified. Age differences were observed in the samples through ANOVA and post-hoc Tukey test. Results Only three metabolites showed a significant difference with age: alanine (Ala) [cubs vs juvenile], free-carnitine (C0) [juvenile vs cubs] and acetylcarnitine (C2) [cubs vs adults and juvenile vs cubs]. Conclusion Metabolites with variability due to age were identified, making them potential biomarkers to monitor metabolic status as early diagnosis in endangered species. This is the first study of black bear amino acid and acylcarnitine profiles, and the values found could be used as reference for free amino acid and acylcarnitine concentrations in further studies of the species.

الإتاحة: https://doi.org/10.1371/journal.pone.0272979Test

المؤلفون: Munoz-Torres, Jose R., Martínez-González, Sidney B., Lozano-Luján, Alan D., Martínez-Vázquez, María C., Velasco-Elizondo, Perla, Garza-Veloz, Idalia, Martinez-Fierro, Margarita L.

المصدر: Frontiers in Bioengineering and Biotechnology ; volume 10 ; ISSN 2296-4185

مصطلحات موضوعية: Biomedical Engineering, Histology, Bioengineering, Biotechnology

الوصف: The amniotic membrane (AM) is the inner part of the placenta. It has been used therapeutically for the last century. The biological proprieties of AM include immunomodulatory, anti-scarring, anti-microbial, pro or anti-angiogenic (surface dependent), and tissue growth promotion. Because of these, AM is a functional tissue for the treatment of different pathologies. The AM is today part of the treatment for various conditions such as wounds, ulcers, burns, adhesions, and skin injury, among others, with surgical resolution. This review focuses on the current surgical areas, including gynecology, plastic surgery, gastrointestinal, traumatology, neurosurgery, and ophthalmology, among others, that use AM as a therapeutic option to increase the success rate of surgical procedures. Currently there are articles describing the mechanisms of action of AM, some therapeutic implications and the use in surgeries of specific surgical areas, this prevents knowing the therapeutic response of AM when used in surgeries of different organs or tissues. Therefore, we described the use of AM in various surgical specialties along with the mechanisms of action, helping to improve the understanding of the therapeutic targets and achieving an adequate perspective of the surgical utility of AM with a particular emphasis on regenerative medicine.

الإتاحة: https://doi.org/10.3389/fbioe.2022.1067480Test

المؤلفون: Avila-Carrasco, Lorena, Díaz-Avila, Daisy Lorena, Reyes-López, Adrian, Monarrez-Espino, Joel, Garza-Veloz, Idalia, Velasco-Elizondo, Perla, Vázquez-Reyes, Sodel, Mauricio-González, Alejandro, Solís-Galván, Jorge Alfonso, Martinez-Fierro, Margarita L.

المصدر: Frontiers in Psychology ; volume 13 ; ISSN 1664-1078

مصطلحات موضوعية: General Psychology

الوصف: Background The social distancing policies implemented by the health authorities during the COVID-19 pandemic in Mexico and elsewhere led to major changes in teaching strategies for college undergraduates. So far, there is limited data regarding the impact of the lockdown on the academic stress and mental health of these students. Objective To assess the occurrence of academic difficulties, anxiety, depression, and academic stressors resulting in somatization with subsequent coping strategies linked to the pandemic. Materials and methods A cross-sectional study was conducted with 728 medical students (years 1–5). A purposely designed questionnaire to assess academic difficulties associated with the pandemic was administered electronically. The validated Goldberg anxiety and depression scale was also used, as well as the SISCO-II inventory on academic stress. Results Screening for anxiety and depression led to a prevalence of 67.9 and 81.3%, respectively. Most relevant stressors, reported always or nearly always, included professors’ evaluations (63.9%), and reading overload of academic papers (50.6%). Factorial analyses showed that women were more prone to stress than men ( p < 0.001). Somatization symptomatology included drowsiness or increased need of sleep, anxiety, anguish, desperation, chronic fatigue, and sleep disorders. Common coping strategies included practicing a hobby, done always or nearly always by 65% of students with high stress, and 34% of those with low stress ( p < 0.001). Conclusion There was a relevant impact of the mandatory lockdown during COVID-19 pandemic on the mental health of medical students reflected in the high prevalence rates of anxiety, depression, and stressors in the studied population pointing to the need for designing and implementing preventive strategies to deal with the effects of lockdowns.

الإتاحة: https://doi.org/10.3389/fpsyg.2022.1066673Test

المؤلفون: Hernández-Rangel, Alejandra E, Hernandez-Fuentes, Gustavo A, Montes-Galindo, Daniel A, Sanchez-Ramirez, Carmen A, Cabrera-Licona, Ariana, Martinez-Fierro, Margarita L, Rodriguez-Sanchez, Iram P, Garza-Veloz, Idalia, Diaz-Martinez, Janet, Casarez-Price, Juan C, Plata-Florenzano, Jorge E, Ochoa-Díaz-Lopez, Hector, Lugo-Trampe, Angel, Delgado-Enciso, Iván

المصدر: Biomedicines ; ISSN:2227-9059 ; Volume:12 ; Issue:6

مصطلحات موضوعية: HPV-16+, TC1 cell line, calcitriol, cervical cancer, neutrophil-to-lymphocyte ratio, vitamin D

الوصف: Vitamin D3 or calcitriol (VitD3) has been shown to have anticancer and anti-inflammatory activity in in vitro models and clinical studies. However, its effect on HPV-16-related cancer has been sparsely explored. In this study, we aimed to determine whether monotherapy or combination therapy with cisplatin (CP) reduces tumor growth and affects survival and systemic inflammation. Treatments were administered to C57BL/6 mice with HPV-16-related tumors (TC-1 cells) as follows: (1) placebo (100 µL vehicle, olive oil, orally administered daily); (2) VitD3 (3.75 µg/kg calcitriol orally administered daily); (3) CP (5 mg/kg intraperitoneally, every 7 days); and (4) VitD3+CP. Tumor growth was monitored for 25 days, survival for 60 days, and the neutrophil-to-lymphocyte ratio (NLR) was evaluated on days 1 (baseline), 7, and 14. VitD3+CP showed greater success in reducing tumor volume compared to CP monotherapy (p = 0.041), while no differences were observed between CP and VitD3 monotherapy (p = 0.671). Furthermore, VitD3+CP prolonged survival compared to CP (p = 0.036) and VitD3 (p = 0.007). Additionally, at day 14 the VitD3 and VitD3+CP groups showed significantly lower NLR values than the CP group (p < 0.05, for both comparisons). Vitamin D3 could be a promising adjuvant in the treatment of cervical cancer or solid tumors and deserves further investigation.

العلاقة: https://doi.org/10.3390/biomedicines12061357Test; https://pubmed.ncbi.nlm.nih.gov/38927564Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11201479Test/

الإتاحة: https://doi.org/10.3390/biomedicines12061357Test
https://pubmed.ncbi.nlm.nih.gov/38927564Test
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11201479Test/

المؤلفون: Vargas-Rodriguez, Jose R., Garza-Veloz, Idalia, Flores-Morales, Virginia, Badillo-Almaraz, Jose I., Rocha-Pizaña, Maria R., Valdés-Aguayo, José J., Martinez-Fierro, Margarita L.

المساهمون: Consejo Nacional de Ciencia y Tecnología

المصدر: Frontiers in Medicine ; volume 8 ; ISSN 2296-858X

الوصف: Since the appearance of the severe acute respiratory syndrome coronavirus (SARS-CoV) in 2003 in China, diabetes mellitus (DM) and hyperglycemia in patients infected with SARS-CoV, represent independent predictors of mortality. Therefore, metabolic control has played a major role in the prognosis of these patients. In the current pandemic of coronavirus disease 19 (COVID-19), multiple studies have shown that DM is one of the main comorbidities associated with COVID-19 and higher risk of complications and death. The incidence and prevalence of COVID-19 complications and death related with hyperglycemia in patients with or without DM are high. There are many hypotheses related with worse prognosis and death related to COVID-19 and/or hyperglycemia. However, the information about the interplay between hyperglycemia and angiotensin-converting enzyme 2 (ACE2), the critical receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in the context of SARS-CoV-2 infection, is almost null, but there is enough information to consider the possible participation of hyperglycemia in the glycation of this protein, unleashing a pool of reactions leading to acute respiratory distress syndrome and death in patients with COVID-19. In this document we investigated the current evidence related with ACE2 as a key element within the pathophysiological mechanism related with hyperglycemia extrapolating it to context of SARS-CoV-2 infection and its relationship with worse prognosis and death for COVID-19.

الإتاحة: https://doi.org/10.3389/fmed.2021.758414Test

المؤلفون: Gómez-Govea, Mayra A., Ramírez-Ahuja, María de Lourdes, Contreras-Perera, Yamili, Jiménez-Camacho, Armando J., Ruiz-Ayma, Gabriel, Villanueva-Segura, Olga Karina, Trujillo-Rodríguez, Gerardo de Jesús, Delgado-Enciso, Iván, Martínez-Fierro, Margarita L., Manrique-Saide, Pablo, Puerta-Guardo, Henry, Flores-Suárez, Adriana E., Ponce-García, Gustavo, Rodríguez-Sánchez, Iram P.

المساهمون: Consejo Nacional de Ciencia y Tecnología

المصدر: Frontiers in Microbiology ; volume 13 ; ISSN 1664-302X

مصطلحات موضوعية: Microbiology (medical), Microbiology

الوصف: Aedes aegypti is a mosquito that transmits viral diseases such as dengue, chikungunya, Zika, and yellow fever. The insect’s microbiota is recognized for regulating several biological processes, including digestion, metabolism, egg production, development, and immune response. However, the role of the bacteria involved in insecticide susceptibility has not been established. Therefore, the objective of this study was to characterize the resident microbiota in a field population of A. aegypti to evaluate its role associated with susceptibility to the insecticides permethrin and deltamethrin. Mosquitoes were fed 10% sucrose mixed with antibiotics and then exposed to insecticides using a diagnostic dose. DNA was extracted, and sequencing of bacterial 16S rRNA was carried out on Illumina ® MiSeq™. Proteobacteria (92.4%) and Bacteroidetes (7.6%) were the phyla, which are most abundant in mosquitoes fed with sucrose 10%. After exposure to permethrin, the most abundant bacterial species were Pantoea agglomerans (38.4%) and Pseudomonas azotoformans-fluorescens-synxantha (14.2%). Elizabethkingia meningoseptica (38.4%) and Ps. azotoformans-fluorescens-synxantha (26.1%) were the most abundant after exposure to deltamethrin. Our results showed a decrease in mosquitoes’ survival when exposed to permethrin, while no difference in survival when exposed to deltamethrin when the microbiota was modified. We found that the change in microbiota modifies the response of mosquitoes to permethrin. These results are essential for a better understanding of mosquito physiology in response to insecticides.

الإتاحة: https://doi.org/10.3389/fmicb.2022.761459Test

المؤلفون: Elizondo-Zertuche, Mariana, de J. Treviño-Rangel, Rogelio, Robledo-Leal, Efrén, Luna-Rodríguez, Carolina E., Martínez-Fierro, Margarita L., Rodríguez-Sánchez, Iram P., González, Gloria M.

المصدر: Mycologia, 2017 Nov 01. 109(6), 874-881.

الوصول الحر: https://www.jstor.org/stable/26592228Test

المؤلفون: Ávila-carrasco, Lorena, García-Mayorga, Elda A., Díaz-Ávila, Daisy L., Garza-Veloz, Idalia, Martínez-Fierro, Margarita L., González-Mateo, Guadalupe T.

مصطلحات موضوعية: Natural plant compounds, Kidney disease, Renoprotective effects, Bioactive compounds, Clinical studies

الوصف: Background: The blockade of the progression or onset of pathological events is essential for the homeostasis of an organism. Some common pathological mechanisms involving a wide range of diseases are the uncontrolled inflammatory reactions that promote fibrosis, oxidative reactions, and other alterations. Natural plant compounds (NPCs) are bioactive elements obtained from natural sources that can regulate physiological processes. Inflammation is recognized as an important factor in the development and evolution of chronic renal damage. Consequently, any compound able to modulate inflammation or inflammation-related processes can be thought of as a renal protective agent and/or a potential treatment tool for controlling renal damage. The objective of this research was to review the beneficial effects of bioactive natural compounds on kidney damage to reveal their efficacy as demonstrated in clinical studies. Methods: This systematic review is based on relevant studies focused on the impact of NPCs with therapeutic potential for kidney disease treatment in humans. Results: Clinical studies have evaluated NPCs as a different way to treat or prevent renal damage and appear to show some benefits in improving OS, inflammation, and antioxidant capacity, therefore making them promising therapeutic tools to reduce or prevent the onset and progression of KD pathogenesis. Conclusions: This review shows the promising clinical properties of NPC in KD therapy. However, more robust clinical trials are needed to establish their safety and therapeutic effects in the area of renal damage.

العلاقة: Publisher's version; http://dx.doi.org/10.3390/molecules26206096Test; Sí; Molecules 26 (2021); http://hdl.handle.net/10261/268092Test

الإتاحة: https://doi.org/10.3390/molecules26206096Test
http://hdl.handle.net/10261/268092Test

المؤلفون: Valdés-Aguayo, José J., Garza-Veloz, Idalia, Vargas-Rodríguez, José R., Martinez-Vazquez, María C., Avila-Carrasco, Lorena, Bernal-Silva, Sofia, González-Fuentes, Carolina, Comas-García, Andreu, Alvarado-Hernández, Diana E., Centeno-Ramirez, Alba S. H., Rodriguez-Sánchez, Iram P., Delgado-Enciso, Ivan, Martinez-Fierro, Margarita L.

المساهمون: Consejo Nacional de Ciencia y Tecnología

المصدر: Frontiers in Cellular and Infection Microbiology ; volume 11 ; ISSN 2235-2988

الوصف: Introduction During severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, the virus hijacks the mitochondria causing damage of its membrane and release of mt-DNA into the circulation which can trigger innate immunity and generate an inflammatory state. In this study, we explored the importance of peripheral blood mt-DNA as an early predictor of evolution in patients with COVID-19 and to evaluate the association between the concentration of mt-DNA and the severity of the disease and the patient’s outcome. Methods A total 102 patients (51 COVID-19 cases and 51 controls) were included in the study. mt-DNA obtained from peripheral blood was quantified by qRT-PCR using the NADH mitochondrial gene. Results There were differences in peripheral blood mt-DNA between patients with COVID-19 (4.25 ng/μl ± 0.30) and controls (3.3 ng/μl ± 0.16) ( p = 0.007). Lower mt-DNA concentrations were observed in patients with severe COVID-19 when compared with mild ( p = 0.005) and moderate ( p = 0.011) cases of COVID-19. In comparison with patients with severe COVID-19 who survived (3.74 ± 0.26 ng/μl) decreased levels of mt-DNA in patients with severe COVID-19 who died (2.4 ± 0.65 ng/μl) were also observed ( p = 0.037). Conclusion High levels of mt-DNA were associated with COVID-19 and its decrease could be used as a potential biomarker to establish a prognosis of severity and mortality of patients with COVID-19.

الإتاحة: https://doi.org/10.3389/fcimb.2021.754708Test