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1دورية أكاديمية
المؤلفون: Yi Hu, Jon G. Sanders, Piotr Łukasik, Catherine L. D’Amelio, John S. Millar, David R. Vann, Yemin Lan, Justin A. Newton, Mark Schotanus, Daniel J. C. Kronauer, Naomi E. Pierce, Corrie S. Moreau, John T. Wertz, Philipp Engel, Jacob A. Russell
المصدر: Nature Communications, Vol 9, Iss 1, Pp 1-14 (2018)
مصطلحات موضوعية: Science
الوصف: Gut bacteria are prevalent across insects including ants, but their precise roles are often unclear. Here, Hu et al. show that microbes aid ants by recycling nitrogen into bio-available amino acids. This function is conserved across the turtle ants, suggesting an ancient nutritional mutualism.
وصف الملف: electronic resource
العلاقة: https://doaj.org/toc/2041-1723Test
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2دورية أكاديمية
المؤلفون: Yi Hu, Jon G. Sanders, Piotr Łukasik, Catherine L. D’Amelio, John S. Millar, David R. Vann, Yemin Lan, Justin A. Newton, Mark Schotanus, Daniel J. C. Kronauer, Naomi E. Pierce, Corrie S. Moreau, John T. Wertz, Philipp Engel, Jacob A. Russell
المصدر: Nature Communications, Vol 9, Iss 1, Pp 1-1 (2018)
مصطلحات موضوعية: Science
الوصف: The originally published version of the Supplementary Information file associated with this Article contained an error in Supplementary Figure 3. Panel b was inadvertently replaced with a duplicate of panel a. The error has now been fixed and the corrected version of the Supplementary Information PDF is available to download from the HTML version of the Article.
وصف الملف: electronic resource
العلاقة: https://doaj.org/toc/2041-1723Test
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3دورية أكاديمية
المؤلفون: Heping Zhou, Kerri Bouwman, Mark Schotanus, Cornelius Verweij, Jorge A Marrero, Deborah Dillon, Jose Costa, Paul Lizardi, Brian B Haab
المساهمون: The Pennsylvania State University CiteSeerX Archives
المصدر: ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/89/cd/Genome_Biol_2004_Mar_30_5(4)_R28.tar.gz
الوصف: Two-color, rolling-circle amplification on antibody microarrays for sensitive, multiplexed serum-protein measurements
وصف الملف: application/zip
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المؤلفون: Brian B. Haab, John M. Trenkle, Kerri Bouwman, Mark Schotanus, Gilbert S. Omenn, Alan W. Partin, Heping Zhou, Robert C. Vogt, Leslie A. Mangold, Samir M. Hanash, Erik Erlandson, David E. Misek, Ji Qiu
المصدر: Proteomics. 3(11)
مصطلحات موضوعية: PCA3, Male, Antibodies, Neoplasm, Protein Array Analysis, Biochemistry, Prostate cancer, Antigen, Antigens, Neoplasm, LNCaP, medicine, Biomarkers, Tumor, Tumor Cells, Cultured, Humans, Molecular Biology, biology, Prostate, Cancer, Prostatic Neoplasms, medicine.disease, Tumor antigen, Data Interpretation, Statistical, Immunology, biology.protein, Cancer research, Protein microarray, Antibody, Chromatography, Liquid
الوصف: The broad characterization of the immune responses elicited by tumors has valuable applications in diagnostics and basic research. We present here the use of microarrays of tumor-derived proteins to profile the antibody repertoire in the sera of prostate cancer patients and controls. Two-dimensional liquid chromatography was used to separate proteins from the prostate cancer cell line LNCaP into 1760 fractions. These fractions were spotted in microarrays on coated microscope slides, and the microarrays were incubated individually with serum samples from 25 men with prostate cancer and 25 male controls. The amount of immunoglobulin bound to each fraction by each serum sample was quantified. Statistical analysis revealed that 38 of the fractions had significantly higher levels of immunoglobulin binding in the prostate cancer samples compared to the controls. Two fractions showed higher binding in the control samples. The significantly higher immunoglobulin reactivity from the prostate cancer samples may reflect a strong immune response to the tumors in the prostate cancer patients. We used multivariate analysis to classify the samples as either prostate cancer or control. In a cross-validation study, recursive partitioning classified the samples with 84% accuracy. A decision tree with two levels of partitioning classified the samples with 98% accuracy. Additional studies will allow further characterization of tumor antigens in prostate cancer and their significance for diagnosis. These results suggest that microarrays of fractionated proteins could be a powerful tool for tumor antigen discovery and cancer diagnosis.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7ea629470ce9c5148034996e3214f82Test
https://pubmed.ncbi.nlm.nih.gov/14595819Test -
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المؤلفون: Paul M. Lizardi, Cornelius Verweij, Brian B. Haab, Jose Costa, Heping Zhou, Jorge A. Marrero, Deborah A. Dillon, Kerri Bouwman, Mark Schotanus
المصدر: Genome Biology. 5:R28
مصطلحات موضوعية: medicine.diagnostic_test, Biology, Blood proteins, Molecular biology, chemistry.chemical_compound, Biotin, chemistry, Rolling circle replication, Immunoassay, Protein Array Analysis, medicine, Protein microarray, Digoxigenin, DNA microarray
الوصف: The ability to conveniently and rapidly profile a diverse set of proteins has valuable applications. In a step toward further enabling such a capability, we developed the use of rolling-circle amplification (RCA) to measure the relative levels of proteins from two serum samples, labeled with biotin and digoxigenin, respectively, that have been captured on antibody microarrays. Two-color RCA produced fluorescence up to 30-fold higher than direct-labeling and indirect-detection methods using antibody microarrays prepared on both polyacrylamide-based hydrogels and nitrocellulose. Replicate RCA measurements of multiple proteins from sets of 24 serum samples were highly reproducible and accurate. In addition, RCA enabled reproducible measurements of distinct expression profiles from lower-abundance proteins that were not measurable using the other detection methods. Two-color RCA on antibody microarrays should allow the convenient acquisition of expression profiles from a great diversity of proteins for a variety of applications.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::7661395c412c3a4f945a3d2a446c5f1aTest
https://doi.org/10.1186/gb-2004-5-4-r28Test -
6دورية أكاديمية
المؤلفون: Kerri Bouwman, Ji Qiu, Heping Zhou, Mark Schotanus, Leslie A. Mangold, Robert Vogt, Erik Erlandson, John Trenkle, Alan W. Partin, David Misek, Gilbert S. Omenn, Brian B. Haab, Samir Hanash
المصدر: Proteomics; Nov2003, Vol. 3 Issue 11, p2200-2207, 8p