المؤلفون: Castven, Darko, Becker, Diana, Czauderna, Carolin, Wilhelm, Diana, Andersen, Jesper B., Strand, Susanne, Hartmann, Monika, Heilmann‐Heimbach, Stefanie, Roth, Wilfried, Hartmann, Nils, Straub, Beate K., Mahn, Friederike L., Franck, Sophia, Pereira, Sharon, Haupts, Anna, Vogel, Arndt, Wörns, Marcus A., Weinmann, Arndt, Heinrich, Stefan, Lang, Hauke, Thorgeirsson, Snorri S., Galle, Peter R., Marquardt, Jens U.

المساهمون: Deutsche Forschungsgemeinschaft, Deutsche Krebshilfe, Volkswagen Foundation, Wilhelm Sander-Stiftung, German Research Foundation, German Cancer Aid

المصدر: International Journal of Cancer ; volume 144, issue 11, page 2782-2794 ; ISSN 0020-7136 1097-0215

الوصف: Primary liver cancer (PLC) ranks among the most lethal solid cancers worldwide due to lack of effective biomarkers for early detection and limited treatment options in advanced stages. Development of primary culture models that closely recapitulate phenotypic and molecular diversities of PLC is urgently needed to improve the patient outcome. Long‐term cultures of 7 primary liver cancer cell lines of hepatocellular and cholangiocellular origin were established using defined culture conditions. Morphological and histological characteristics of obtained cell lines and xenograft tumors were analyzed and compared to original tumors. Time course analyses of transcriptomic and genomic changes were performed using next‐generation sequencing (NGS). Key oncogenic alterations were identified by targeted NGS and cell lines carrying potentially actionable mutations were treated with corresponding specific inhibitors. PDCL fully resembled morphological features of the primary cancers in vitro and in vivo over extended period in culture. Genomic alterations as well as transcriptome profiles showed high similarity with primary tumors and remained stable during long‐term culturing. Targeted‐NGS confirmed that key oncogenic mutations such as TP53, KRAS, CTNNB1 as well as actionable mutations (e.g. MET, cKIT, KDR) were highly conserved in PDCL and amenable for individualized therapeutic approaches. Integrative genomic and transcriptomic approaches further demonstrated that PDCL more closely resemble molecular and prognostic features of PLC than established cell lines and are valuable tool for direct target evaluation. Our integrative analysis demonstrates that PDCL represents refined model for discovery of relevant molecular subgroups and exploration of precision medicine approaches for the treatment of this deadly disease.

الإتاحة: https://doi.org/10.1002/ijc.32026Test

المؤلفون: Czauderna, Carolin, Palestino-Dominguez, Mayrel, Castven, Darko, Becker, Diana, Zanon-Rodriguez, Luis, Hajduk, Jovana, Mahn, Friederike L., Herr, Monika, Strand, Dennis, Strand, Susanne, Heilmann-Heimbach, Stefanie, Gomez-Quiroz, Luis E., Wörns, Marcus A., Galle, Peter R., Marquardt, Jens U.

المساهمون: Pizzo, Salvatore V, Johannes Gutenberg-University of Mainz, Dr. Willmar Schwabe GmBH

المصدر: PLOS ONE ; volume 13, issue 12, page e0209067 ; ISSN 1932-6203

الإتاحة: https://doi.org/10.1371/journal.pone.0209067Test

المؤلفون: Szczepanowski, Monika, Lange, Jonas, Kohler, Christian W., Masque‐Soler, Neus, Zimmermann, Martin, Aukema, Sietse M., Altenbuchinger, Michael, Rehberg, Thorsten, Mahn, Friederike, Siebert, Reiner, Spang, Rainer, Burkhardt, Birgit, Klapper, Wolfram

المساهمون: KinderKrebsInitiative Buchholz/Holm-Seppensen, Medical Faculty of the University of Kiel to WK, Cells-in-Motion Cluster, German Ministry of Health, Foundation de Drie Lichten, JSM-Ubbo Emmius Foundation Talent Grant

المصدر: British Journal of Haematology ; volume 179, issue 1, page 116-119 ; ISSN 0007-1048 1365-2141

الوصف: Summary We present the largest series of diffuse large B‐cell lymphoma (DLBCL) in patients younger than 18 years analysed to date by gene expression profiling using Nanostring technology to identify molecular subtypes and fluorescent in situ hybridization for translocations of MYC . We show that the activated B cell‐like subtype of DLBCL is exceedingly rare in children and – in contrast to adults‐ not associated with outcome. Furthermore, we review the current literature and demonstrate that MYC translocations are not more frequent in paediatric compared to adult DLBCL. A prognostic role of MYC in the paediatric age groups seems unlikely.

الإتاحة: https://doi.org/10.1111/bjh.14812Test

المؤلفون: Mahn, Friederike Marie

الإتاحة: http://nbn-resolving.de/urn:nbn:de:gbv:8-diss-50191Test

المؤلفون: Mahn, Friederike Marie

الوصول الحر: https://www.europeana.eu/item/2048441/item_WKYDBF6KGGC6T7CUYKNQUORSG4WHX7NR?utm_source=api&utm_medium=api&utm_campaign=YuvuWBeCaTest

المؤلفون: Förster, Matti, Mahn, Friederike, Gockel, Ulrich, Brosz, Mathias, Freynhagen, Rainer, Tölle, Thomas R., Baron, Ralf

المساهمون: Eldabe, Sam

المصدر: PLoS ONE ; volume 8, issue 7, page e68273 ; ISSN 1932-6203

الإتاحة: https://doi.org/10.1371/journal.pone.0068273Test

المؤلفون: Mahn, Friederike, Hüllemann, Philipp, Gockel, Ulrich, Brosz, Mathias, Freynhagen, Rainer, Tölle, Thomas R., Baron, Ralf

المساهمون: Priller, Josef

المصدر: PLoS ONE ; volume 6, issue 5, page e18018 ; ISSN 1932-6203

الإتاحة: https://doi.org/10.1371/journal.pone.0018018Test

المؤلفون: Castven, Darko, Becker, Diana, Czauderna, Carolin, Wilhelm, Diana, Andersen, Jesper B, Strand, Susanne, Hartmann, Monika, Heilmann-Heimbach, Stefanie, Roth, Wilfried, Hartmann, Nils, Straub, Beate K, Mahn, Friederike L, Franck, Sophia, Pereira, Sharon, Haupts, Anna, Vogel, Arndt, Wörns, Marcus A, Weinmann, Arndt, Heinrich, Stefan, Lang, Hauke, Thorgeirsson, Snorri S, Galle, Peter R, Marquardt, Jens U

المصدر: Castven , D , Becker , D , Czauderna , C , Wilhelm , D , Andersen , J B , Strand , S , Hartmann , M , Heilmann-Heimbach , S , Roth , W , Hartmann , N , Straub , B K , Mahn , F L , Franck , S , Pereira , S , Haupts , A , Vogel , A , Wörns , M A , Weinmann , A , Heinrich , S , Lang , H , Thorgeirsson , S S , Galle , P R & Marquardt , J U 2019 ....

الوصف: Primary liver cancer (PLC) ranks among the most lethal solid cancers worldwide due to lack of effective biomarkers for early detection and limited treatment options in advanced stages. Development of primary culture models that closely recapitulate phenotypic and molecular diversities of PLC is urgently needed to improve the patient outcome. Long-term cultures of 7 primary liver cancer cell lines of hepatocellular and cholangiocellular origin were established using defined culture conditions. Morphological and histological characteristics of obtained cell lines and xenograft tumors were analyzed and compared to original tumors. Time course analyses of transcriptomic and genomic changes were performed using next-generation sequencing (NGS). Key oncogenic alterations were identified by targeted NGS and cell lines carrying potentially actionable mutations were treated with corresponding specific inhibitors. PDCL fully resembled morphological features of the primary cancers in vitro and in vivo over extended period in culture. Genomic alterations as well as transcriptome profiles showed high similarity with primary tumors and remained stable during long-term culturing. Targeted-NGS confirmed that key oncogenic mutations such as TP53, KRAS, CTNNB1 as well as actionable mutations (e.g. MET, cKIT, KDR) were highly conserved in PDCL and amenable for individualized therapeutic approaches. Integrative genomic and transcriptomic approaches further demonstrated that PDCL more closely resemble molecular and prognostic features of PLC than established cell lines and are valuable tool for direct target evaluation. Our integrative analysis demonstrates that PDCL represents refined model for discovery of relevant molecular subgroups and exploration of precision medicine approaches for the treatment of this deadly disease. This article is protected by copyright. All rights reserved.

الإتاحة: https://doi.org/10.1002/ijc.32026Test
https://curis.ku.dk/portal/da/publications/application-of-patientderived-liver-cancer-cells-for-phenotypic-characterization-and-therapeutic-target-identificationTest(1fb6fd57-eb9b-436b-bf53-c1ab0cf4a0db).html

المؤلفون: Lewinska, Monika, Bhatt, Deepak Kumar, Satriano, Letizia, Oliveira, Douglas VNP, Mahn, Friederike, Marquardt, Jens, Matter, Matthias, Perugorria, María Jesús, María Banales, Jesús, Andersen, Jesper

المصدر: Journal of Hepatology ; volume 70, issue 1, page e368 ; ISSN 0168-8278

مصطلحات موضوعية: Hepatology

الإتاحة: https://doi.org/10.1016/s0618-8278Test(19)30720-0
https://api.elsevier.com/content/article/PII:S0618827819307200?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0618827819307200?httpAccept=text/plainTest

المؤلفون: Regitz, Charlotte, Fitzenberger, Elena, Mahn, Friederike Luise, Dußling, Lisa Marie, Wenzel, Uwe

المصدر: European Journal of Nutrition ; volume 55, issue 2, page 741-747 ; ISSN 1436-6207 1436-6215

مصطلحات موضوعية: Nutrition and Dietetics, Medicine (miscellaneous)

الإتاحة: https://doi.org/10.1007/s00394-015-0894-1Test